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The map label for this gene is rffA [C]

Identifier: 116327887

GI number: 116327887

Start: 1384530

End: 1385693

Strand: Direct

Name: rffA [C]

Synonym: LBL_1172

Alternate gene names: 116327887

Gene position: 1384530-1385693 (Clockwise)

Preceding gene: 116327886

Following gene: 116327888

Centisome position: 38.31

GC content: 40.21

Gene sequence:

>1164_bases
ATGCCCGGATTTGAACTAATAGGAAATGAAGAGTTTGGAGAATTGGAAGATTTATTTCAAAGGAGTAAAATACTTTTTCG
GCATGGATTTGACGGGATTCGGAATGGGGTTTTTAAGGTAAGAGAATTTGAACTTGCTTTTGCGAAACATTTCGGGTTTC
AGGATGCCCTAGCGGTTACTTCCGGAACTGCAGCTTTGAAAGTAGCGATGAAAGCTGCTAATATTAAACCTGGGGATGAG
GTGATCACCCAGTCTTTTACATTCTTAGCAACTGTGGAAGCGATTGTAGAGGCAGGTGCTACTCCAATCATTTGTGAGAT
AGATGAATCTCTCAATATGGATCCAATTGCTCTTGAAAAATTGATCACTCAAAAGACTAGGATGATTGTTCCAGTGCATA
TGTTAGGTGTCCCCGCAAGGATGGGTCAAATTCTCGCGATTGCCCGAAAGTATAATCTTTTAGTTATGGAGGATACTGCT
TGGGGATGTGGCGGAAAATTTCAGGGCTCATACCTCGGAACTTTAGGAGATATTGGTAGTTTTAGTTTTGATTTTGCAAA
GATCATAACTACTGGTGAAGGTGGAATGATACTTGCAAAGGATCAGAGTTTATTGGAAAGAGCTAAAGCATATCATGATC
ATGGGCACGAAAATAACCCAAAGTTACCTCGCTGGGAAGACTCTAGAAGTAATTCGGGTTTTAACTATCGAATGACAGAA
ATGCAGGGAGCCGTTGGTCTTGCTCAACTTAAAAAGTTGGGTGAAATCGTGAAGTTGCAAAGAGCTAATTTAGAGAAAAT
TAAATCGGTTGTATCTCGATATGAGGGTATCCGTTTTAGAGATGTTCCCGAGTCCGGTGAAGAAACTGCTGACGCTTTGG
TCTTTTTTACAAAAACTCCAAAATTGGCGCGTGCTTTTCGGCTAGCGATGCTTGAAGAAGGTCTTGGTACTAAAATACTC
CCTGAAGCTATTTCTTGGCATTTTGCTGGAACTTGGAATCATATTCCCGAACTTGTTCGTCAGTATCCTAATTTGTTCGA
AGCTTTTCCACGCTCTTTAGAATTATTAGATAGAAGCGTAGCCATCCCAATTCAAGTAAAGATGCAAGATCAAGTCTTGG
ATAAAGTGGATTCTGCCATGATTAAGGTGTTTAAAGGTAATTAA

Upstream 100 bases:

>100_bases
TCTTCCAAATTGGATTATCTCTAAAAAGGAATTTTAAAGATCAGTTCTAATTGATTTCGTCGCGGTCAAAAATCTCGATT
GAGCAAATAGGAAAATAAGT

Downstream 100 bases:

>100_bases
GAATGAAGATATTAGGTATTAATGCATTCGGACATGATACGTCAGCTGCTCTCGTTATCGATGGGAAATTGATTGCGGCA
GTAGAAGAGGAACGGTTGAA

Product: pyridoxal phosphate-dependent aminotransferase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPGFELIGNEEFGELEDLFQRSKILFRHGFDGIRNGVFKVREFELAFAKHFGFQDALAVTSGTAALKVAMKAANIKPGDE
VITQSFTFLATVEAIVEAGATPIICEIDESLNMDPIALEKLITQKTRMIVPVHMLGVPARMGQILAIARKYNLLVMEDTA
WGCGGKFQGSYLGTLGDIGSFSFDFAKIITTGEGGMILAKDQSLLERAKAYHDHGHENNPKLPRWEDSRSNSGFNYRMTE
MQGAVGLAQLKKLGEIVKLQRANLEKIKSVVSRYEGIRFRDVPESGEETADALVFFTKTPKLARAFRLAMLEEGLGTKIL
PEAISWHFAGTWNHIPELVRQYPNLFEAFPRSLELLDRSVAIPIQVKMQDQVLDKVDSAMIKVFKGN

Sequences:

>Translated_387_residues
MPGFELIGNEEFGELEDLFQRSKILFRHGFDGIRNGVFKVREFELAFAKHFGFQDALAVTSGTAALKVAMKAANIKPGDE
VITQSFTFLATVEAIVEAGATPIICEIDESLNMDPIALEKLITQKTRMIVPVHMLGVPARMGQILAIARKYNLLVMEDTA
WGCGGKFQGSYLGTLGDIGSFSFDFAKIITTGEGGMILAKDQSLLERAKAYHDHGHENNPKLPRWEDSRSNSGFNYRMTE
MQGAVGLAQLKKLGEIVKLQRANLEKIKSVVSRYEGIRFRDVPESGEETADALVFFTKTPKLARAFRLAMLEEGLGTKIL
PEAISWHFAGTWNHIPELVRQYPNLFEAFPRSLELLDRSVAIPIQVKMQDQVLDKVDSAMIKVFKGN
>Mature_386_residues
PGFELIGNEEFGELEDLFQRSKILFRHGFDGIRNGVFKVREFELAFAKHFGFQDALAVTSGTAALKVAMKAANIKPGDEV
ITQSFTFLATVEAIVEAGATPIICEIDESLNMDPIALEKLITQKTRMIVPVHMLGVPARMGQILAIARKYNLLVMEDTAW
GCGGKFQGSYLGTLGDIGSFSFDFAKIITTGEGGMILAKDQSLLERAKAYHDHGHENNPKLPRWEDSRSNSGFNYRMTEM
QGAVGLAQLKKLGEIVKLQRANLEKIKSVVSRYEGIRFRDVPESGEETADALVFFTKTPKLARAFRLAMLEEGLGTKILP
EAISWHFAGTWNHIPELVRQYPNLFEAFPRSLELLDRSVAIPIQVKMQDQVLDKVDSAMIKVFKGN

Specific function: Involved In Eca Elongation. [C]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=226, Percent_Identity=38.4955752212389, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI145693159, Length=231, Percent_Identity=34.6320346320346, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 43088; Mature: 42957

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGFELIGNEEFGELEDLFQRSKILFRHGFDGIRNGVFKVREFELAFAKHFGFQDALAVT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCHHHHHHC
SGTAALKVAMKAANIKPGDEVITQSFTFLATVEAIVEAGATPIICEIDESLNMDPIALEK
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LITQKTRMIVPVHMLGVPARMGQILAIARKYNLLVMEDTAWGCGGKFQGSYLGTLGDIGS
HHHHHHHEEEEHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHCCCC
FSFDFAKIITTGEGGMILAKDQSLLERAKAYHDHGHENNPKLPRWEDSRSNSGFNYRMTE
CCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHH
MQGAVGLAQLKKLGEIVKLQRANLEKIKSVVSRYEGIRFRDVPESGEETADALVFFTKTP
HHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCH
KLARAFRLAMLEEGLGTKILPEAISWHFAGTWNHIPELVRQYPNLFEAFPRSLELLDRSV
HHHHHHHHHHHHCCCCCCCCHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
AIPIQVKMQDQVLDKVDSAMIKVFKGN
ECEEEEEEHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
PGFELIGNEEFGELEDLFQRSKILFRHGFDGIRNGVFKVREFELAFAKHFGFQDALAVT
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHCCCHHHHHHC
SGTAALKVAMKAANIKPGDEVITQSFTFLATVEAIVEAGATPIICEIDESLNMDPIALEK
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHH
LITQKTRMIVPVHMLGVPARMGQILAIARKYNLLVMEDTAWGCGGKFQGSYLGTLGDIGS
HHHHHHHEEEEHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHCCCC
FSFDFAKIITTGEGGMILAKDQSLLERAKAYHDHGHENNPKLPRWEDSRSNSGFNYRMTE
CCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEHH
MQGAVGLAQLKKLGEIVKLQRANLEKIKSVVSRYEGIRFRDVPESGEETADALVFFTKTP
HHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCH
KLARAFRLAMLEEGLGTKILPEAISWHFAGTWNHIPELVRQYPNLFEAFPRSLELLDRSV
HHHHHHHHHHHHCCCCCCCCHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
AIPIQVKMQDQVLDKVDSAMIKVFKGN
ECEEEEEEHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]