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The map label for this gene is ilvD

Identifier: 116327733

GI number: 116327733

Start: 1151102

End: 1152784

Strand: Direct

Name: ilvD

Synonym: LBL_0987

Alternate gene names: 116327733

Gene position: 1151102-1152784 (Clockwise)

Preceding gene: 116327730

Following gene: 116327736

Centisome position: 31.85

GC content: 47.77

Gene sequence:

>1683_bases
ATGAGCGATATATTAAAAAAAAGAAGTTCGATGACTACGGACGGAGATAACCGCGCGCCCAATCGAGCGATGCTTCGCGC
GGTCGGCTTTACGGACGAAGATTTCCACAAACCTATGATCGGAATCGCTTCCACATGGAGCGAAGTGACGCCTTGTAATA
TTCATATCAATAAGTTGGCCGAAAAAGTCAAAGAGGGCGTTCGTACGGCGGGAGGAGTTCCTCAAATTTACGGAACCATC
ACGGTTTCCGACGGAATTACGATGGGTCACGAAGGGATGCATTTTTCACTTCCCTCCAGAGAAGTGATTGCGGATTCAAT
CGAAATCGTTTCCAACGCGATGAGACACGACGGAGTGATCGCGATCGGAGGTTGTGATAAGAACATGCCCGGATGTCTCA
TGGCTCTTTGCAGAATCGATGCTCCTTCTATTTTCGTTTACGGGGGGACCATTCTTCCGGGGCATTGCGACGGTCAAGAC
GTGGATATCGTTTCCATCTTCGAAGCTGTTGGTAAATTTAATGCAGGTAAAATTTCAAGAGAAGAATTCATTCGAATCGA
GCAGAACGCGTGTCCCGGCGCAGGAAGTTGCGGGGGGATGTATACCGCAAATACGATGTCCTCCGCGATAGAAGCTCTTG
GGATGAGTCTGCCAGGTTCGGCTTCCATGCCCGCGGTGAGTTCTAGAAAATCGGAGGATTGTTACGAGGCGGGAAAAGCT
CTCATCAATCTCATTCAAAAGGGAGTCACTCCGAAGCGAATTCTTACCAAAAAAGCTTTTGAGAACGCGATCACCGTAGT
GCTTGTGTTAGGCGGTTCAACCAATGCGGTCCTTCATTTGATTGCGATCGCGAAAGAGATTGGTGTGGATCTTACTCTCG
AAGACTTTGATCGAATCAGTAAAAAAACTCCTCACCTTGCGGATTTGAAACCGGGTGGTAGATACGCCATGACCGATCTC
GACAAAGTGGGCGGGGTTCATGGTGTGATGAAGTATCTTTTGAAAGAAGGAATGCTTCATGGTGATTGTCTTACGGTTAC
GGGAAAAACAATTGCAGAAAATCTAATGGATATGCCCGATCTCGTTCCAAACCAGACGATCGTCCGTAAAAAATCGGAGG
CGCTCCATCCTTCCGGTCCTCTTGTGATTTTGAAAGGAAACCTTGCCCCGGAAGGTGCGGTTGCTAAAATTTCCGGTTTG
AAGAAAATTTCCATCACGGGCCCGGCGAAAGTGTTCGAATCCGAAGACGATTGTTTCAATGCGATCATGACGGATCAAAT
TAAACCGGGCGACGTGATTATCATTCGTTACGAAGGTCCCAAAGGTGGTCCCGGGATGAGAGAGATGCTCGCGGTAACTT
CGGCGCTTGTCGGAAAAGGTTTGGGAGAAGACGTGGGTCTGATGACAGACGGTCGATTCAGTGGAGGAACGCACGGTCTC
GTAGTCGGTCATATTTCTCCCGAAGCATTTGATGGAGGTCCGATTGCGATCGTTCAAAATGGGGATGCGGTTACTATCGA
TTCCGGTAAAAATTTGCTCCAAGTTGAAATTTCTCAGGAAGAAATTCAGAAACGGCTAAAAAATTGGAAACCGATTGAAC
CGAGATATAAATCCGGGGTTCTTGCGAAATACGCAAAACTGGTTCAATCGGCGACTAACGGAGCGATTACGAATCTGCTT
TGA

Upstream 100 bases:

>100_bases
TGAGCTTTTCCATTTGACCGGAAGGGAGAGTCCGCAAAACTCTACTTCTATAACTCGTCCAAAACGAACGTTTTAGGAAC
GACTAAGTTAGAGGCTACCT

Downstream 100 bases:

>100_bases
AAACGGTCCATTGTGAACCCATCGTAGACGACAATAACTCCCTTTGAGATCGGTTACGTTTTTTCCCTTGATAAAAGAAT
TTACGGTTCTACGAAGACGA

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSDILKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEVTPCNIHINKLAEKVKEGVRTAGGVPQIYGTI
TVSDGITMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVIAIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGHCDGQD
VDIVSIFEAVGKFNAGKISREEFIRIEQNACPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKSEDCYEAGKA
LINLIQKGVTPKRILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVDLTLEDFDRISKKTPHLADLKPGGRYAMTDL
DKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLMDMPDLVPNQTIVRKKSEALHPSGPLVILKGNLAPEGAVAKISGL
KKISITGPAKVFESEDDCFNAIMTDQIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
VVGHISPEAFDGGPIAIVQNGDAVTIDSGKNLLQVEISQEEIQKRLKNWKPIEPRYKSGVLAKYAKLVQSATNGAITNLL

Sequences:

>Translated_560_residues
MSDILKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEVTPCNIHINKLAEKVKEGVRTAGGVPQIYGTI
TVSDGITMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVIAIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGHCDGQD
VDIVSIFEAVGKFNAGKISREEFIRIEQNACPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKSEDCYEAGKA
LINLIQKGVTPKRILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVDLTLEDFDRISKKTPHLADLKPGGRYAMTDL
DKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLMDMPDLVPNQTIVRKKSEALHPSGPLVILKGNLAPEGAVAKISGL
KKISITGPAKVFESEDDCFNAIMTDQIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
VVGHISPEAFDGGPIAIVQNGDAVTIDSGKNLLQVEISQEEIQKRLKNWKPIEPRYKSGVLAKYAKLVQSATNGAITNLL
>Mature_559_residues
SDILKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEVTPCNIHINKLAEKVKEGVRTAGGVPQIYGTIT
VSDGITMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVIAIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGHCDGQDV
DIVSIFEAVGKFNAGKISREEFIRIEQNACPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKSEDCYEAGKAL
INLIQKGVTPKRILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVDLTLEDFDRISKKTPHLADLKPGGRYAMTDLD
KVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLMDMPDLVPNQTIVRKKSEALHPSGPLVILKGNLAPEGAVAKISGLK
KISITGPAKVFESEDDCFNAIMTDQIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGLV
VGHISPEAFDGGPIAIVQNGDAVTIDSGKNLLQVEISQEEIQKRLKNWKPIEPRYKSGVLAKYAKLVQSATNGAITNLL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=39.3092105263158, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI2367371, Length=457, Percent_Identity=35.6673960612691, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1786464, Length=481, Percent_Identity=33.4719334719335, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1788157, Length=509, Percent_Identity=28.4872298624754, Blast_Score=192, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=48.75, Blast_Score=543, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_LEPBJ (Q04RA5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801323.1
- ProteinModelPortal:   Q04RA5
- SMR:   Q04RA5
- STRING:   Q04RA5
- GeneID:   4410841
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2063
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- PhylomeDB:   Q04RA5
- ProtClustDB:   PRK00911
- BioCyc:   LBOR355277:LBJ_2063-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59446; Mature: 59314

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDILKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEVTPCNIHINKLA
CCHHHHHHHCCCCCCCCCCCCHHHHEEECCCCHHHHCCHHHHHHCCCCCCCCEEEHHHHH
EKVKEGVRTAGGVPQIYGTITVSDGITMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVI
HHHHHHHHHCCCCCEEEEEEEEECCEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCEE
AIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGHCDGQDVDIVSIFEAVGKFNAGKISR
EECCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCH
EEFIRIEQNACPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKSEDCYEAGKA
HHHEEEECCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LINLIQKGVTPKRILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVDLTLEDFDRIS
HHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEHHHHHHHH
KKTPHLADLKPGGRYAMTDLDKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLMDMPD
HCCCCCCCCCCCCCEEECCHHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCC
LVPNQTIVRKKSEALHPSGPLVILKGNLAPEGAVAKISGLKKISITGPAKVFESEDDCFN
CCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCCHHHHCCCEEEEECCCHHHHCCCCHHHH
AIMTDQIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCE
VVGHISPEAFDGGPIAIVQNGDAVTIDSGKNLLQVEISQEEIQKRLKNWKPIEPRYKSGV
EEEECCCCCCCCCCEEEEECCCEEEECCCCCEEEEEECHHHHHHHHHCCCCCCCHHHCCH
LAKYAKLVQSATNGAITNLL
HHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
SDILKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEVTPCNIHINKLA
CHHHHHHHCCCCCCCCCCCCHHHHEEECCCCHHHHCCHHHHHHCCCCCCCCEEEHHHHH
EKVKEGVRTAGGVPQIYGTITVSDGITMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVI
HHHHHHHHHCCCCCEEEEEEEEECCEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCEE
AIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGHCDGQDVDIVSIFEAVGKFNAGKISR
EECCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCH
EEFIRIEQNACPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKSEDCYEAGKA
HHHEEEECCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LINLIQKGVTPKRILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVDLTLEDFDRIS
HHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCEEHHHHHHHH
KKTPHLADLKPGGRYAMTDLDKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLMDMPD
HCCCCCCCCCCCCCEEECCHHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHCCCC
LVPNQTIVRKKSEALHPSGPLVILKGNLAPEGAVAKISGLKKISITGPAKVFESEDDCFN
CCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCCHHHHCCCEEEEECCCHHHHCCCCHHHH
AIMTDQIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCE
VVGHISPEAFDGGPIAIVQNGDAVTIDSGKNLLQVEISQEEIQKRLKNWKPIEPRYKSGV
EEEECCCCCCCCCCEEEEECCCEEEECCCCCEEEEEECHHHHHHHHHCCCCCCCHHHCCH
LAKYAKLVQSATNGAITNLL
HHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA