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The map label for this gene is speE [H]

Identifier: 116327324

GI number: 116327324

Start: 568782

End: 570278

Strand: Direct

Name: speE [H]

Synonym: LBL_0517

Alternate gene names: 116327324

Gene position: 568782-570278 (Clockwise)

Preceding gene: 116327323

Following gene: 116327325

Centisome position: 15.74

GC content: 41.95

Gene sequence:

>1497_bases
TTGCAAACAGCGCTTTATATTTCGGTTCTTATCATATCTTCTTGCGGTCTCATTTACGAACTGTTAGCTGGGACGGTTGC
GTCGTATCTGCTCGGGGAAACGGTCACACAATTCTCTCTCATTATAGGAACGTATTTGTTTTCTATGGGGGTCGGTTCCT
GGTTATCCAAGTATATGGAAAAGGATCTGATTCCTAAATTTTTGGAAATCGAACTTGCAATCGGACTTGTGGGCGGTTTC
GGTTCGGCGATTCTTTATTTGAGTTTCGGGCAAATTCGGTACTTTCAGATTCCGTTGTTCTTGCTTGTGATTTTAATCGG
AGTTTTGGTAGGACTTGAAATACCGGTTTTGTTAAGAATCTTGAAAAAAGAACTGCAATTCAAGGAACTCGTCTCGAGGG
TTTTGAGTTTGGATTACGTAGGTGCGTTGCTCGCATCGATTTTATTTCCGATTTTCTTCGCGCCTAAGTTAGGTTTGATA
AGAACCGGATTTCTATTCGGAATTTTGAATGCAGGAGTGGCGGTTTGGGGAACTTGGGTGCTTCCTCTTAAACATTCCAG
AATGATTTTGCTGCGAGCGCAATCCGTTGTCGTATTAACGCTTTTGATCCTAGGATTTTCCTTTTCCGATTTGATCACAC
ATTACAGCGAGGAATCCTTATACACGGATGAGATCATACTTTCGAAACAATCTCGGTTTCAGAGGATCATCGTGACATGC
TGGAAAAACGAAATTCGACTTTTTTTAAATGGACATCTTCAATTTTCATCCAGGGATGAATATCGTTATCACGAAACGCT
GGTTCATCCAGCATTACTTGCACACCCGGCTCCTAAAAGAGTATTGGTTTTGGGAGGAGGGGACGGGCTTGCCGTGAGGG
AGATTCTAAAACACAGAAATGTAGAATCGATTACGTTAGTCGACTTGGATCCGACGGTTACCGACTTGTTTGCCGAACAC
GAGTTTTTAAAGGAGCTTAACGAGGAAAGTTTAAGGGATCCTAAAGTGAAAGTGATCAATACGGACGCATTCATTTGGTT
GGAGGAAAACGAACAAATCTTCGATGTGGTCATCATTGATTTTCCGGACCCGAGTAATTTTTCCTTGGGAAAACTTTATA
CCACGGCGTTTTTTCATACGTTAAAAAGAAGAATGAATGAAACGTCCGTTCTCGAAATACAATCCACATCACCTCTTTTT
GCCCGTTCTTCGTATTGGTGCATCGAAAGAACAATCGCTTTTTTAGGTTTTCATACACTTCCTCTGCACGTTTACGTTCC
CTCTTTCGGAGAATGGGGGTTTGTTCTGGCGGGTCAAAAGCCGATTCGGTTCCGAAAAGAGTTTCCGGAACATCTTAGAT
TTTTGAATATTCAAGAACTTGAATCTATTCAGGTTTTTCCTCAGGACATGTCTCGAATTCCGGTGGATATCAATCGACTG
AATAATCAAGCGCTTGTTCGATACTATGACCGGGAATGGAATCGAATCCTGGATTGA

Upstream 100 bases:

>100_bases
GAGGTGGAAAATGTTCCTAAGACCGTGCGGGAGAATCCGGGAGTATATCGCTCCCACTATACGAATTTTATGAGATATTC
CGGGGGAAAGTAGGGCCCGT

Downstream 100 bases:

>100_bases
AAAGGAAGGAATCCTGGCGGAGGGTCCTGCGCGAATCCATATGAAATCAAAATTCACCTAAAACGGCAGAATCGTGCGCC
CAGTCTTCGATCAAAAAAAT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MQTALYISVLIISSCGLIYELLAGTVASYLLGETVTQFSLIIGTYLFSMGVGSWLSKYMEKDLIPKFLEIELAIGLVGGF
GSAILYLSFGQIRYFQIPLFLLVILIGVLVGLEIPVLLRILKKELQFKELVSRVLSLDYVGALLASILFPIFFAPKLGLI
RTGFLFGILNAGVAVWGTWVLPLKHSRMILLRAQSVVVLTLLILGFSFSDLITHYSEESLYTDEIILSKQSRFQRIIVTC
WKNEIRLFLNGHLQFSSRDEYRYHETLVHPALLAHPAPKRVLVLGGGDGLAVREILKHRNVESITLVDLDPTVTDLFAEH
EFLKELNEESLRDPKVKVINTDAFIWLEENEQIFDVVIIDFPDPSNFSLGKLYTTAFFHTLKRRMNETSVLEIQSTSPLF
ARSSYWCIERTIAFLGFHTLPLHVYVPSFGEWGFVLAGQKPIRFRKEFPEHLRFLNIQELESIQVFPQDMSRIPVDINRL
NNQALVRYYDREWNRILD

Sequences:

>Translated_498_residues
MQTALYISVLIISSCGLIYELLAGTVASYLLGETVTQFSLIIGTYLFSMGVGSWLSKYMEKDLIPKFLEIELAIGLVGGF
GSAILYLSFGQIRYFQIPLFLLVILIGVLVGLEIPVLLRILKKELQFKELVSRVLSLDYVGALLASILFPIFFAPKLGLI
RTGFLFGILNAGVAVWGTWVLPLKHSRMILLRAQSVVVLTLLILGFSFSDLITHYSEESLYTDEIILSKQSRFQRIIVTC
WKNEIRLFLNGHLQFSSRDEYRYHETLVHPALLAHPAPKRVLVLGGGDGLAVREILKHRNVESITLVDLDPTVTDLFAEH
EFLKELNEESLRDPKVKVINTDAFIWLEENEQIFDVVIIDFPDPSNFSLGKLYTTAFFHTLKRRMNETSVLEIQSTSPLF
ARSSYWCIERTIAFLGFHTLPLHVYVPSFGEWGFVLAGQKPIRFRKEFPEHLRFLNIQELESIQVFPQDMSRIPVDINRL
NNQALVRYYDREWNRILD
>Mature_498_residues
MQTALYISVLIISSCGLIYELLAGTVASYLLGETVTQFSLIIGTYLFSMGVGSWLSKYMEKDLIPKFLEIELAIGLVGGF
GSAILYLSFGQIRYFQIPLFLLVILIGVLVGLEIPVLLRILKKELQFKELVSRVLSLDYVGALLASILFPIFFAPKLGLI
RTGFLFGILNAGVAVWGTWVLPLKHSRMILLRAQSVVVLTLLILGFSFSDLITHYSEESLYTDEIILSKQSRFQRIIVTC
WKNEIRLFLNGHLQFSSRDEYRYHETLVHPALLAHPAPKRVLVLGGGDGLAVREILKHRNVESITLVDLDPTVTDLFAEH
EFLKELNEESLRDPKVKVINTDAFIWLEENEQIFDVVIIDFPDPSNFSLGKLYTTAFFHTLKRRMNETSVLEIQSTSPLF
ARSSYWCIERTIAFLGFHTLPLHVYVPSFGEWGFVLAGQKPIRFRKEFPEHLRFLNIQELESIQVFPQDMSRIPVDINRL
NNQALVRYYDREWNRILD

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=254, Percent_Identity=28.3464566929134, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1786312, Length=182, Percent_Identity=35.7142857142857, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645616, Length=183, Percent_Identity=33.879781420765, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25154106, Length=182, Percent_Identity=34.0659340659341, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6325326, Length=241, Percent_Identity=32.3651452282158, Blast_Score=107, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323175, Length=233, Percent_Identity=30.0429184549356, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645443, Length=229, Percent_Identity=30.1310043668122, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355941, Length=175, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 56986; Mature: 56986

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTALYISVLIISSCGLIYELLAGTVASYLLGETVTQFSLIIGTYLFSMGVGSWLSKYME
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
KDLIPKFLEIELAIGLVGGFGSAILYLSFGQIRYFQIPLFLLVILIGVLVGLEIPVLLRI
HHCCCHHHHHEEEEEECCCHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHCCHHHHHHH
LKKELQFKELVSRVLSLDYVGALLASILFPIFFAPKLGLIRTGFLFGILNAGVAVWGTWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHEEEE
LPLKHSRMILLRAQSVVVLTLLILGFSFSDLITHYSEESLYTDEIILSKQSRFQRIIVTC
EEECCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
WKNEIRLFLNGHLQFSSRDEYRYHETLVHPALLAHPAPKRVLVLGGGDGLAVREILKHRN
HCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCC
VESITLVDLDPTVTDLFAEHEFLKELNEESLRDPKVKVINTDAFIWLEENEQIFDVVIID
CCEEEEEECCCHHHHHHHHHHHHHHHCHHHCCCCCEEEEECCEEEEECCCCEEEEEEEEE
FPDPSNFSLGKLYTTAFFHTLKRRMNETSVLEIQSTSPLFARSSYWCIERTIAFLGFHTL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHEEE
PLHVYVPSFGEWGFVLAGQKPIRFRKEFPEHLRFLNIQELESIQVFPQDMSRIPVDINRL
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCCCCCHHHC
NNQALVRYYDREWNRILD
CCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTALYISVLIISSCGLIYELLAGTVASYLLGETVTQFSLIIGTYLFSMGVGSWLSKYME
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
KDLIPKFLEIELAIGLVGGFGSAILYLSFGQIRYFQIPLFLLVILIGVLVGLEIPVLLRI
HHCCCHHHHHEEEEEECCCHHHHHHHEEECCEEEEHHHHHHHHHHHHHHHHCCHHHHHHH
LKKELQFKELVSRVLSLDYVGALLASILFPIFFAPKLGLIRTGFLFGILNAGVAVWGTWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHEEEE
LPLKHSRMILLRAQSVVVLTLLILGFSFSDLITHYSEESLYTDEIILSKQSRFQRIIVTC
EEECCCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
WKNEIRLFLNGHLQFSSRDEYRYHETLVHPALLAHPAPKRVLVLGGGDGLAVREILKHRN
HCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCC
VESITLVDLDPTVTDLFAEHEFLKELNEESLRDPKVKVINTDAFIWLEENEQIFDVVIID
CCEEEEEECCCHHHHHHHHHHHHHHHCHHHCCCCCEEEEECCEEEEECCCCEEEEEEEEE
FPDPSNFSLGKLYTTAFFHTLKRRMNETSVLEIQSTSPLFARSSYWCIERTIAFLGFHTL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHEEE
PLHVYVPSFGEWGFVLAGQKPIRFRKEFPEHLRFLNIQELESIQVFPQDMSRIPVDINRL
EEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCCCCCHHHC
NNQALVRYYDREWNRILD
CCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]