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The map label for this gene is rpoA

Identifier: 116327291

GI number: 116327291

Start: 526888

End: 527865

Strand: Direct

Name: rpoA

Synonym: LBL_0480

Alternate gene names: 116327291

Gene position: 526888-527865 (Clockwise)

Preceding gene: 116327290

Following gene: 116327292

Centisome position: 14.58

GC content: 44.27

Gene sequence:

>978_bases
TTGTCTCTCAAAAGCTTACTCAAAGGATTTAAACGTCCTAAGAAGATTGAATTCAACACGGAAGCGAGCACTCCCAATTA
TGGGAAGTTCGTTGCAGAGCCTTTTGAAAGGGGTTTTGCGACAACGATCGGTAACTCTCTTAGAAGGACTTTGATGTCCT
CGATTGAGGGAGCCGCGATTTCTGCGATTCGTATCGAAGGGGTAAACCACGAGTTTTCCTTTATCGAAGGAGTTGCGGAA
GACGTAACAAGAATTATTCTGAACCTGAAACAGGTTCGGATTAAATACGAGCCTGAAGAAAAGGATCAGAGTAAGATCAT
TCATCTGGAATTGAAAGGAGCCGGATATTTCAGAGCGGGAGATCTCGCCGTGGATTCTTCCATAGAAATCATGAATCCCG
ACCTTCACATTGCTACTCTGAACGAAGACGCGAATCTGGTGATGGATTTGGAAATTCAGAGAGGAAGAGGATACGTTCCT
GCGGAAGAAAAGAAAAAAGATATCGAAGTTCTCGGGACGATTCCTGTGGATTCGATCTTTTCTCCCGTTCAGAAAGTGGT
TTTTGAAGTATCCGAAACTCGCGTTGCTCAAAGATCGGATTACGAAAAACTGACTCTGGAAGTATGGACTGACGGTTCCG
TGTCTCCGGACGACGCCGTGGCTCAAGCTGCGAAAATCTTAAAAGAGCATCTTACGGTATTTATCAATTTCGAAGAGGAA
TTGGAGGAAGAAGACGACGAACTGGATGAAGCGGACGAAAAACTCAAGGCTTCCTTGTCGAAACACGTGGAAGAATTGGA
GCTTTCCGTTCGTTCTCTCAATGTGTTGAGAAGTCTGGAAATCGATTTCATCGGGGACTTGGTCAAAAGATCCGAAGAAG
AAATGTCGAAGTCCAAACACTACAGTGATCAGTGTCTCCAAGAGTTGAAGGGAAAACTTTCCACTCTGGGTCTCTCTTTT
GGAATGAGGGATTTTTAA

Upstream 100 bases:

>100_bases
GTTACGGCATTACCAGAGCGTCATCATATCGATCTACCGGTGAAAGAACAGGTAATCGTAGAGCTTTATTCGAAATAAAA
TCTGATAGAAGGGTCTCAAA

Downstream 100 bases:

>100_bases
AATGAATAAAAGAAATAAAGTAAAACATCTAAACCGCAATAAAGGTCATAGAGACGCGTTGATCAATAACATGATCACCT
CTCTTTTTAAATACGAGAGA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSLKSLLKGFKRPKKIEFNTEASTPNYGKFVAEPFERGFATTIGNSLRRTLMSSIEGAAISAIRIEGVNHEFSFIEGVAE
DVTRIILNLKQVRIKYEPEEKDQSKIIHLELKGAGYFRAGDLAVDSSIEIMNPDLHIATLNEDANLVMDLEIQRGRGYVP
AEEKKKDIEVLGTIPVDSIFSPVQKVVFEVSETRVAQRSDYEKLTLEVWTDGSVSPDDAVAQAAKILKEHLTVFINFEEE
LEEEDDELDEADEKLKASLSKHVEELELSVRSLNVLRSLEIDFIGDLVKRSEEEMSKSKHYSDQCLQELKGKLSTLGLSF
GMRDF

Sequences:

>Translated_325_residues
MSLKSLLKGFKRPKKIEFNTEASTPNYGKFVAEPFERGFATTIGNSLRRTLMSSIEGAAISAIRIEGVNHEFSFIEGVAE
DVTRIILNLKQVRIKYEPEEKDQSKIIHLELKGAGYFRAGDLAVDSSIEIMNPDLHIATLNEDANLVMDLEIQRGRGYVP
AEEKKKDIEVLGTIPVDSIFSPVQKVVFEVSETRVAQRSDYEKLTLEVWTDGSVSPDDAVAQAAKILKEHLTVFINFEEE
LEEEDDELDEADEKLKASLSKHVEELELSVRSLNVLRSLEIDFIGDLVKRSEEEMSKSKHYSDQCLQELKGKLSTLGLSF
GMRDF
>Mature_324_residues
SLKSLLKGFKRPKKIEFNTEASTPNYGKFVAEPFERGFATTIGNSLRRTLMSSIEGAAISAIRIEGVNHEFSFIEGVAED
VTRIILNLKQVRIKYEPEEKDQSKIIHLELKGAGYFRAGDLAVDSSIEIMNPDLHIATLNEDANLVMDLEIQRGRGYVPA
EEKKKDIEVLGTIPVDSIFSPVQKVVFEVSETRVAQRSDYEKLTLEVWTDGSVSPDDAVAQAAKILKEHLTVFINFEEEL
EEEDDELDEADEKLKASLSKHVEELELSVRSLNVLRSLEIDFIGDLVKRSEEEMSKSKHYSDQCLQELKGKLSTLGLSFG
MRDF

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=39.1304347826087, Blast_Score=224, Evalue=5e-60,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_LEPBJ (Q04PW5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801813.1
- ProteinModelPortal:   Q04PW5
- STRING:   Q04PW5
- GeneID:   4411353
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2632
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   LBOR355277:LBJ_2632-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36618; Mature: 36487

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKSLLKGFKRPKKIEFNTEASTPNYGKFVAEPFERGFATTIGNSLRRTLMSSIEGAAI
CCHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEE
SAIRIEGVNHEFSFIEGVAEDVTRIILNLKQVRIKYEPEEKDQSKIIHLELKGAGYFRAG
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCEEEEC
DLAVDSSIEIMNPDLHIATLNEDANLVMDLEIQRGRGYVPAEEKKKDIEVLGTIPVDSIF
CEEECCCCEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCHHCCCCEEEEECCCHHHHH
SPVQKVVFEVSETRVAQRSDYEKLTLEVWTDGSVSPDDAVAQAAKILKEHLTVFINFEEE
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECHHH
LEEEDDELDEADEKLKASLSKHVEELELSVRSLNVLRSLEIDFIGDLVKRSEEEMSKSKH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
YSDQCLQELKGKLSTLGLSFGMRDF
CHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLKSLLKGFKRPKKIEFNTEASTPNYGKFVAEPFERGFATTIGNSLRRTLMSSIEGAAI
CHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEE
SAIRIEGVNHEFSFIEGVAEDVTRIILNLKQVRIKYEPEEKDQSKIIHLELKGAGYFRAG
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCEEEEC
DLAVDSSIEIMNPDLHIATLNEDANLVMDLEIQRGRGYVPAEEKKKDIEVLGTIPVDSIF
CEEECCCCEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCHHCCCCEEEEECCCHHHHH
SPVQKVVFEVSETRVAQRSDYEKLTLEVWTDGSVSPDDAVAQAAKILKEHLTVFINFEEE
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECHHH
LEEEDDELDEADEKLKASLSKHVEELELSVRSLNVLRSLEIDFIGDLVKRSEEEMSKSKH
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
YSDQCLQELKGKLSTLGLSFGMRDF
CHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA