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The map label for this gene is yneJ [H]

Identifier: 116249303

GI number: 116249303

Start: 693285

End: 694190

Strand: Direct

Name: yneJ [H]

Synonym: pRL120638

Alternate gene names: 116249303

Gene position: 693285-694190 (Clockwise)

Preceding gene: 116249299

Following gene: 116249304

Centisome position: 79.69

GC content: 62.91

Gene sequence:

>906_bases
TTGGACCTCTCATCGATCGAGATATTCCTCGCCGTTGCCAGCGACCGTAGTGTCACCAAGGCTGCCAAGGCTGTCGGGCG
GGTTCCGTCGAATGTGACGACGCGCGTCCAGCAATTGGAGGAGGATCTCGGCGTTTCCCTCTTCAGCCGTGACGGTAAGA
AGATGACGTTGACACGCGAGGGTGAGACGTTTCTCGGCTATGCCAACCGGCTTATGGCGCTTGCGCTCGAAGCGCGCCAG
GCCGTGCGGCCTCTCGCCCCATCGGGGACGTTGCGGGTCGGCACCATGGAGAGCACGGCGGCAAGCAGGCTGCCGGCGGC
GCTGACGCAATTCAATCAGATGTGGCCTGATGTGTCGCTTCATCTGACGATGGGAGCGTCCCGCGATCTGACCCGCGACG
TCCTGGCTGATGTCCTGGATTGCGCGCTGATTGCCCGCCCGCCGAAGACGATGCGGGAGGAAGATTCAGGTTTCGATGCC
GAACTCAAGGCGCTCGAGATCGAGCCGGTCTTCGTCGAGGACCTGTTGATCGTCTTGCCGTCCGGGCATCCCTCTATCAA
ATCCGCCGCCGACCTGCGTGTCGGGTCGCTCGCCGCTCTAGAGCCCGGCTGCACCTATCGCCGGATCGCCGAAAACTGGG
CGCGCAAATCGAGCGCGCTGCCGACGAGCGAACTCGGCTCCTACCACGCGATCCTGGCGAGCGTTGCGACCGGGAATACG
GCGGGTGTCATGCCGCGATCCGTCCTTGACCTCATGCATTGGCCGACGCCTGTCCAGACCCATCAGCTAGGCCCTGTCGA
GACGTTGCTCGTCTACCGCAAGCATGATCGCCCCAGCGCGTTCAACGCATTCCACGAAGTTCTCAGCGCGACAAAGGGCA
GAGATGTCAGGCTGGCGACAAACTAG

Upstream 100 bases:

>100_bases
GGCGTTTTTTCGTCCGATGCCGAAACCGGCTTGAGCTGATGCAATGATTTTTATATAGAGAATTATCTATCGTCTTAATT
CCGGAAACGAGATCTTTTCA

Downstream 100 bases:

>100_bases
CCTGCACGCCTCTCTCCTTCGCCGCCAGATAATCCTCGCTCAAATTGGTCTTGTCATGCAGTATCCCGTTTCCCCGAAGG
TGGGACCGAGCCGCTTCCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRVQQLEEDLGVSLFSRDGKKMTLTREGETFLGYANRLMALALEARQ
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPDVSLHLTMGASRDLTRDVLADVLDCALIARPPKTMREEDSGFDA
ELKALEIEPVFVEDLLIVLPSGHPSIKSAADLRVGSLAALEPGCTYRRIAENWARKSSALPTSELGSYHAILASVATGNT
AGVMPRSVLDLMHWPTPVQTHQLGPVETLLVYRKHDRPSAFNAFHEVLSATKGRDVRLATN

Sequences:

>Translated_301_residues
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRVQQLEEDLGVSLFSRDGKKMTLTREGETFLGYANRLMALALEARQ
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPDVSLHLTMGASRDLTRDVLADVLDCALIARPPKTMREEDSGFDA
ELKALEIEPVFVEDLLIVLPSGHPSIKSAADLRVGSLAALEPGCTYRRIAENWARKSSALPTSELGSYHAILASVATGNT
AGVMPRSVLDLMHWPTPVQTHQLGPVETLLVYRKHDRPSAFNAFHEVLSATKGRDVRLATN
>Mature_301_residues
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRVQQLEEDLGVSLFSRDGKKMTLTREGETFLGYANRLMALALEARQ
AVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPDVSLHLTMGASRDLTRDVLADVLDCALIARPPKTMREEDSGFDA
ELKALEIEPVFVEDLLIVLPSGHPSIKSAADLRVGSLAALEPGCTYRRIAENWARKSSALPTSELGSYHAILASVATGNT
AGVMPRSVLDLMHWPTPVQTHQLGPVETLLVYRKHDRPSAFNAFHEVLSATKGRDVRLATN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=297, Percent_Identity=36.3636363636364, Blast_Score=179, Evalue=1e-46,
Organism=Escherichia coli, GI1790208, Length=272, Percent_Identity=27.5735294117647, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082132, Length=254, Percent_Identity=28.740157480315, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI157672245, Length=181, Percent_Identity=28.1767955801105, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693105, Length=144, Percent_Identity=27.0833333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=185, Percent_Identity=28.1081081081081, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788481, Length=147, Percent_Identity=27.891156462585, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788626, Length=120, Percent_Identity=32.5, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790399, Length=225, Percent_Identity=23.1111111111111, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32655; Mature: 32655

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRVQQLEEDLGVSLFSRDGKKMTLTRE
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCEEEEEEC
GETFLGYANRLMALALEARQAVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPDVSL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEE
HLTMGASRDLTRDVLADVLDCALIARPPKTMREEDSGFDAELKALEIEPVFVEDLLIVLP
EEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCEECCCEEEEEC
SGHPSIKSAADLRVGSLAALEPGCTYRRIAENWARKSSALPTSELGSYHAILASVATGNT
CCCCCCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
AGVMPRSVLDLMHWPTPVQTHQLGPVETLLVYRKHDRPSAFNAFHEVLSATKGRDVRLAT
CCCCHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEC
N
C
>Mature Secondary Structure
MDLSSIEIFLAVASDRSVTKAAKAVGRVPSNVTTRVQQLEEDLGVSLFSRDGKKMTLTRE
CCCCCEEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECCCCCEEEEEEC
GETFLGYANRLMALALEARQAVRPLAPSGTLRVGTMESTAASRLPAALTQFNQMWPDVSL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCEE
HLTMGASRDLTRDVLADVLDCALIARPPKTMREEDSGFDAELKALEIEPVFVEDLLIVLP
EEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCEECCCEEEEEC
SGHPSIKSAADLRVGSLAALEPGCTYRRIAENWARKSSALPTSELGSYHAILASVATGNT
CCCCCCCHHHHCCCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
AGVMPRSVLDLMHWPTPVQTHQLGPVETLLVYRKHDRPSAFNAFHEVLSATKGRDVRLAT
CCCCHHHHHHHHHCCCCCCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]