Click here to switch to the map view.

The map label for this gene is 116249285

Identifier: 116249285

GI number: 116249285

Start: 674118

End: 674786

Strand: Direct

Name: 116249285

Synonym: pRL120620

Alternate gene names: NA

Gene position: 674118-674786 (Clockwise)

Preceding gene: 116249284

Following gene: 116249286

Centisome position: 77.48

GC content: 58.59

Gene sequence:

>669_bases
ATGACGACGTTCCGTCCGAAATACATCACCTTCGACTGCTATGGCACGCTGACCAACTTTCAGATGGCGGAAGCGGCGCG
CGACCTCTACGGCGAAGAACTCGACGAGCCACGCATGGCGGAGTTCATCAAGAATTTCGCCGCCTATCGGCTCGACGAGA
TCTTGGGCGACTGGAAACCCTATGCCGAGGTGGTGCACAATTCGCTCGAGCGGACATGCAAGCGCAACGGCGTGACCTTT
CGCGATGAAGCCGCCCGGATGATCTATGAGCGGGTTCCGAGCTGGGGGCCGCATTCGGATGTTCCAGCCGGTCTCGCCAA
GGTCGCCAAGGAAATCCCTTTGGTCATCCTCTCCAACGCCATGAATTCGCAGATCATGTCGAACGTCGAAAAACTGGGCG
CGCCCTTCCATGCGGTCTATACCGCCGAGCAGGCGCAGTCCTATAAGCCACGCTTCAAGGGCTTTGAATATATGCTCGAT
ATGCTGGGCTGCGGGCCGGAAGACGTGCTGCATTGCTCGTCCTCCTTCCGCTACGACCTGATGTCGGCCCACGATCTCGG
CATCAAGAACAAGGTTTGGGTCAATCGTGGCCACGAGCCGGCCAATCCCTACTACGGTTATGTCGAAATCCCTGACATCT
CGGGCCTGCCTGGCGTCGTCGGGCTCTGA

Upstream 100 bases:

>100_bases
CGATACGATAGATTCTGCTTCCGATGCCGCCAATTCAGGCGAGTTGCGGGCGAAGCGATGCCTATGATTGTGATAGTTCC
GATCGTTCAAGGATACGCAG

Downstream 100 bases:

>100_bases
CGGAGACACGCGGATGCAGTTCGAATCCTACTGGCACGACACGGCACCAGCCTTTGCCGGCGGCGCTCAAGGCCCCGTCG
AAGGACATTACGACGTCGCC

Product: putative haloacid dehydrogenase

Products: (R)-2-hydroxyacid; halide

Alternate protein names: HAD Family Hydrolase; Hydrolase; Haloalkanoic Acid Dehalogenase; Dehalogenase; 2-Haloacid Dehalogenase; Haloacid Dehalogenase-Like Hydrolase; Haloacid Dehalogenase; Haloacid Dehalogenase I; 2-Haloalkanoic Acid Dehalogenase; Haloacid Dehydrogenase; 2-Haloacid Halidohydrolase Type II; HAD-Superfamily Hydrolase; Haloacid Dehalogenase Type II Truncated; 2-Haloacid Halidohydrolase IVa; 2-Haloacid Halidohydrolase IVA; Dehalogenase Protein; HAD Superfamily Haloalkanoic Acid Dehalogenase

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MTTFRPKYITFDCYGTLTNFQMAEAARDLYGEELDEPRMAEFIKNFAAYRLDEILGDWKPYAEVVHNSLERTCKRNGVTF
RDEAARMIYERVPSWGPHSDVPAGLAKVAKEIPLVILSNAMNSQIMSNVEKLGAPFHAVYTAEQAQSYKPRFKGFEYMLD
MLGCGPEDVLHCSSSFRYDLMSAHDLGIKNKVWVNRGHEPANPYYGYVEIPDISGLPGVVGL

Sequences:

>Translated_222_residues
MTTFRPKYITFDCYGTLTNFQMAEAARDLYGEELDEPRMAEFIKNFAAYRLDEILGDWKPYAEVVHNSLERTCKRNGVTF
RDEAARMIYERVPSWGPHSDVPAGLAKVAKEIPLVILSNAMNSQIMSNVEKLGAPFHAVYTAEQAQSYKPRFKGFEYMLD
MLGCGPEDVLHCSSSFRYDLMSAHDLGIKNKVWVNRGHEPANPYYGYVEIPDISGLPGVVGL
>Mature_221_residues
TTFRPKYITFDCYGTLTNFQMAEAARDLYGEELDEPRMAEFIKNFAAYRLDEILGDWKPYAEVVHNSLERTCKRNGVTFR
DEAARMIYERVPSWGPHSDVPAGLAKVAKEIPLVILSNAMNSQIMSNVEKLGAPFHAVYTAEQAQSYKPRFKGFEYMLDM
LGCGPEDVLHCSSSFRYDLMSAHDLGIKNKVWVNRGHEPANPYYGYVEIPDISGLPGVVGL

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.8.1.2

Molecular weight: Translated: 25119; Mature: 24988

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFRPKYITFDCYGTLTNFQMAEAARDLYGEELDEPRMAEFIKNFAAYRLDEILGDWKP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCH
YAEVVHNSLERTCKRNGVTFRDEAARMIYERVPSWGPHSDVPAGLAKVAKEIPLVILSNA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
MNSQIMSNVEKLGAPFHAVYTAEQAQSYKPRFKGFEYMLDMLGCGPEDVLHCSSSFRYDL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHH
MSAHDLGIKNKVWVNRGHEPANPYYGYVEIPDISGLPGVVGL
HHHHCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TTFRPKYITFDCYGTLTNFQMAEAARDLYGEELDEPRMAEFIKNFAAYRLDEILGDWKP
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCH
YAEVVHNSLERTCKRNGVTFRDEAARMIYERVPSWGPHSDVPAGLAKVAKEIPLVILSNA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
MNSQIMSNVEKLGAPFHAVYTAEQAQSYKPRFKGFEYMLDMLGCGPEDVLHCSSSFRYDL
HHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHH
MSAHDLGIKNKVWVNRGHEPANPYYGYVEIPDISGLPGVVGL
HHHHCCCCCCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-haloacid; H2O

Specific reaction: (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA