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The map label for this gene is gbsB [H]

Identifier: 116249267

GI number: 116249267

Start: 653032

End: 654261

Strand: Direct

Name: gbsB [H]

Synonym: pRL120602

Alternate gene names: 116249267

Gene position: 653032-654261 (Clockwise)

Preceding gene: 116249266

Following gene: 116249268

Centisome position: 75.06

GC content: 66.67

Gene sequence:

>1230_bases
ATGAGCCTCTTTGCCGCCCTGAGGCTGCCGCAGGAAATCCTGTTTGGCGTCGGCCAGCGCCATGCGCTCGCGAGCGTCGC
CAGCAGAACCGGCCGGCGCGCACTCGTCTGCACGGATGCGCGTTTTGCCGCAACACCGGTCTTCGCGGAGATGATCGCCG
CGCTCGAAGCAGCCGGCATTGTCGTGCTGGTGCATGACCAGACGCTGCCGGACGTGCCGCAAGATACGGTCGCGGTTTGC
GTTGAGGCCGCACGCGGTTTTGAGCCCGATATGGTGATCGGCATCGGCGGCGGCAGCTGTCTCGACATGGCGAAATGCGC
TTCGCTCCTGCTCGCTCACGGCGGCGCGCTCGCCGATTATTACGGTGAGTTCAAGGTGCCGGCGCCCGTTCTGCCGGTCA
TCGCCGTGCCGACGACGGCAGGCACGGGCTCCGAGGTGACGCCGGTCGCGGTTGTTTCCGATCCTGACCGTATCCTGAAG
GTTGGCATTTCCAGCCCATATCTCATCGCCGCAGCGGCGATCTGCGACCCGGAACTGACCTTGTCCTGCCCGCCTGGGCT
TACGGCGATTGCCGGTGCGGATGCGTTGACGCATGCCATCGAAGCCTTCACGGCCATGCGCCGCCCCGGCGATCCTGAAC
TCGCGCAGCAGCATGTCTTCGTCGGCAAGAGCGATCTCTCCGACCAGTTCGCGCTGCATGCCATCCGGCTCCTCAGCCGG
AGCTTGGAAAAGGCCTTCGTCGACGGCTCCGACATCGATGCGCGCGCCGATGTGATGATGGGCGCGCTCGCAGCCGGCTG
CGCCTTCGGAACGGCGGGCACCGCTGCGGCCCATGCCGTGCAATATCCGGTCGGTGCCTTGACCCATACGCCGCACGGCC
TCGGGGTTGCGACCATGCTGCCCTATGTGATGACATATAATCGCCGCATCGCGAGCGCGGACATCGCCATTGTCGGTAGG
GCGCTCGGCCTCGATCCGACCGGAGTGGAAGACGACGCAACGGTTGCCGATGCCGCGATCGGCGAGGTGAGCCGCCTCTT
CGCCGCGATCGGCATCGCGCCGACACTTGCCGGGCTCGGCCTGCCGGAAGACAGGATCGACTGGGCCGCGGAGCAGGCGC
TCGGCATCGGCCGGCTGATCAAGAACAATCCCCGCTCCTTCGATGCGGCCGCCATGCGCGGCTTGGTACGTGCCGCCTAT
GACGGCGACCTGGCCGCTTCCGCCCTCTGA

Upstream 100 bases:

>100_bases
CGGCGGCGAGCCGCAACGGGTTCGAGATCGACCAGCGCCTCTGGGAAGACCTCAACGCGCTTTCGCGCCCTCGCATTCTG
GCCTTCGAGGGACAAGGATC

Downstream 100 bases:

>100_bases
CCTGGGAAACCATCATGAACCTCAACCAGCATTCTCCGCATCCAGACCACGATTCCTTCGAGCTCAGCGCGTTCTCCCGC
GGCCTCTATATTGGCGGCGC

Product: putative alcohol dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSLFAALRLPQEILFGVGQRHALASVASRTGRRALVCTDARFAATPVFAEMIAALEAAGIVVLVHDQTLPDVPQDTVAVC
VEAARGFEPDMVIGIGGGSCLDMAKCASLLLAHGGALADYYGEFKVPAPVLPVIAVPTTAGTGSEVTPVAVVSDPDRILK
VGISSPYLIAAAAICDPELTLSCPPGLTAIAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALHAIRLLSR
SLEKAFVDGSDIDARADVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATMLPYVMTYNRRIASADIAIVGR
ALGLDPTGVEDDATVADAAIGEVSRLFAAIGIAPTLAGLGLPEDRIDWAAEQALGIGRLIKNNPRSFDAAAMRGLVRAAY
DGDLAASAL

Sequences:

>Translated_409_residues
MSLFAALRLPQEILFGVGQRHALASVASRTGRRALVCTDARFAATPVFAEMIAALEAAGIVVLVHDQTLPDVPQDTVAVC
VEAARGFEPDMVIGIGGGSCLDMAKCASLLLAHGGALADYYGEFKVPAPVLPVIAVPTTAGTGSEVTPVAVVSDPDRILK
VGISSPYLIAAAAICDPELTLSCPPGLTAIAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALHAIRLLSR
SLEKAFVDGSDIDARADVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATMLPYVMTYNRRIASADIAIVGR
ALGLDPTGVEDDATVADAAIGEVSRLFAAIGIAPTLAGLGLPEDRIDWAAEQALGIGRLIKNNPRSFDAAAMRGLVRAAY
DGDLAASAL
>Mature_408_residues
SLFAALRLPQEILFGVGQRHALASVASRTGRRALVCTDARFAATPVFAEMIAALEAAGIVVLVHDQTLPDVPQDTVAVCV
EAARGFEPDMVIGIGGGSCLDMAKCASLLLAHGGALADYYGEFKVPAPVLPVIAVPTTAGTGSEVTPVAVVSDPDRILKV
GISSPYLIAAAAICDPELTLSCPPGLTAIAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALHAIRLLSRS
LEKAFVDGSDIDARADVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATMLPYVMTYNRRIASADIAIVGRA
LGLDPTGVEDDATVADAAIGEVSRLFAAIGIAPTLAGLGLPEDRIDWAAEQALGIGRLIKNNPRSFDAAAMRGLVRAAYD
GDLAASAL

Specific function: Essential for the utilization of choline as a precursor [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=375, Percent_Identity=28.5333333333333, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI48994951, Length=342, Percent_Identity=32.1637426900585, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082107, Length=323, Percent_Identity=36.2229102167183, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1789163, Length=373, Percent_Identity=30.2949061662198, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787493, Length=314, Percent_Identity=34.3949044585987, Blast_Score=132, Evalue=6e-32,
Organism=Escherichia coli, GI1789386, Length=364, Percent_Identity=24.4505494505494, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17537053, Length=400, Percent_Identity=26.75, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321181, Length=402, Percent_Identity=29.3532338308458, Blast_Score=166, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24657991, Length=389, Percent_Identity=31.3624678663239, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.1 [H]

Molecular weight: Translated: 41965; Mature: 41833

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFAALRLPQEILFGVGQRHALASVASRTGRRALVCTDARFAATPVFAEMIAALEAAGI
CCHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCE
VVLVHDQTLPDVPQDTVAVCVEAARGFEPDMVIGIGGGSCLDMAKCASLLLAHGGALADY
EEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHH
YGEFKVPAPVLPVIAVPTTAGTGSEVTPVAVVSDPDRILKVGISSPYLIAAAAICDPELT
CCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCHHHEEECCCCCHHHHHHHHCCCCCE
LSCPPGLTAIAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALHAIRLLSR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHH
SLEKAFVDGSDIDARADVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATML
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHCCCCCCCCHHHHH
PYVMTYNRRIASADIAIVGRALGLDPTGVEDDATVADAAIGEVSRLFAAIGIAPTLAGLG
HHHHHHCCHHCCCHHHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPEDRIDWAAEQALGIGRLIKNNPRSFDAAAMRGLVRAAYDGDLAASAL
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SLFAALRLPQEILFGVGQRHALASVASRTGRRALVCTDARFAATPVFAEMIAALEAAGI
CHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCE
VVLVHDQTLPDVPQDTVAVCVEAARGFEPDMVIGIGGGSCLDMAKCASLLLAHGGALADY
EEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHH
YGEFKVPAPVLPVIAVPTTAGTGSEVTPVAVVSDPDRILKVGISSPYLIAAAAICDPELT
CCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCCHHHEEECCCCCHHHHHHHHCCCCCE
LSCPPGLTAIAGADALTHAIEAFTAMRRPGDPELAQQHVFVGKSDLSDQFALHAIRLLSR
EECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHH
SLEKAFVDGSDIDARADVMMGALAAGCAFGTAGTAAAHAVQYPVGALTHTPHGLGVATML
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHCCCCCCCCHHHHH
PYVMTYNRRIASADIAIVGRALGLDPTGVEDDATVADAAIGEVSRLFAAIGIAPTLAGLG
HHHHHHCCHHCCCHHHEEHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPEDRIDWAAEQALGIGRLIKNNPRSFDAAAMRGLVRAAYDGDLAASAL
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8752328; 9384377 [H]