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The map label for this gene is ybiC [C]

Identifier: 116249177

GI number: 116249177

Start: 557851

End: 558855

Strand: Reverse

Name: ybiC [C]

Synonym: pRL120513

Alternate gene names: 116249177

Gene position: 558855-557851 (Counterclockwise)

Preceding gene: 116249178

Following gene: 116249176

Centisome position: 64.23

GC content: 63.38

Gene sequence:

>1005_bases
ATGGCCGGGCAGAGGATAAGAATGCCGATCGGCGAGCTCCGCCACCTGGCGCTCGAAGCCTGCCTCGCCTGCGGCGGCAG
CCCGGCCATGGCGAAGGCGCTCGTCGATGCGACGCTGTCGGCCGCCTGCTTCGGGCGCACTGAGCTCGGTTTTCCGCATT
TCGTCGATTATCTCACCAGTCTGCGCGACGGGCGGATCAATGGAGACGCCAAGCCCCGCTTCAACCATGTGCTGCCGGCC
CTGATCCAGGCCGATGCGGACGGCGGCATAGCGCAGCTCGGGTTTGACCTCATTTACGACGACTTCACGACGCGCGTGAA
AACCTTCGGCATTTCAGTCTTCACGCAAAGAAACAGCTATACCGCCGGGGAATTGGGATATTACGTCCGGCGTCTCGCCC
AGGACGGGTTGATATCCATCGCCGCCGCCAACGGACCGGCGTTGATGGCGGCGGCCGAGGGCGGGGAGCGCGTCTATTGC
ACCAATCCGCTCGCTTTCGGCCTGCCTCTGCCCGAACCCTTGCCGCCCCTCGTCATCGACCAGGCGACGAGTGCCACGGC
CTTCGTAACTCTTGCCGAGGCGGCAAAGGAACAGTCGCCGATCGCTCACGGCATGGCCATCGACGAGACCGGCGCAATCA
CCACCGATCCGGTCAAGGCGATGCTCGGCGCTCTCCTGCCCTTCGGCGGCTATAAGGGCGCCAATATCGCCCTTATCGTC
GAGATGCTTTCTGCGGGTCTTTCCGGTGCCGCGTGGTCGCTCGATGCCGGCCACTTTCTTTCGGGCGAACACCCGGTGAA
TGCGGGAATGACCGTGATCGCGCTATTCCCCATGGCCGCGGATTCGGATTTCCCGGAGCGCGCCAAGGCCCAATTGGAAC
GGCTGCGTTCCAAGGGAGTCCGCATCCCCGGAGACAGAGCGATGCCCGCGAGCGTCACGGAAACCGATAGCCTTGAAATC
GATATAGGCGTGTTGGAAACGATCCGTCGCTTGCGCCTTCAGTAA

Upstream 100 bases:

>100_bases
GGAAGTCGGCCGCCTGACGCCTGGGAATCACGATGTCAAAACTCTTCTCGCGTCTGTTTTCGCTGATCGGCCTGGGCGGA
CGAGCCGCATTGGAGCTGTG

Downstream 100 bases:

>100_bases
GAAAATCATTACTCAACTCAAAGAACCGAATGCATCGTGCCCGGCGGCATCATGCCGAAGTTCCCATCGTTGGTGCGCAT
TGGGAAATTGAAGATCTCTA

Product: putative malate/lactate dehydrogenase family protein

Products: oxaloacetate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAGQRIRMPIGELRHLALEACLACGGSPAMAKALVDATLSAACFGRTELGFPHFVDYLTSLRDGRINGDAKPRFNHVLPA
LIQADADGGIAQLGFDLIYDDFTTRVKTFGISVFTQRNSYTAGELGYYVRRLAQDGLISIAAANGPALMAAAEGGERVYC
TNPLAFGLPLPEPLPPLVIDQATSATAFVTLAEAAKEQSPIAHGMAIDETGAITTDPVKAMLGALLPFGGYKGANIALIV
EMLSAGLSGAAWSLDAGHFLSGEHPVNAGMTVIALFPMAADSDFPERAKAQLERLRSKGVRIPGDRAMPASVTETDSLEI
DIGVLETIRRLRLQ

Sequences:

>Translated_334_residues
MAGQRIRMPIGELRHLALEACLACGGSPAMAKALVDATLSAACFGRTELGFPHFVDYLTSLRDGRINGDAKPRFNHVLPA
LIQADADGGIAQLGFDLIYDDFTTRVKTFGISVFTQRNSYTAGELGYYVRRLAQDGLISIAAANGPALMAAAEGGERVYC
TNPLAFGLPLPEPLPPLVIDQATSATAFVTLAEAAKEQSPIAHGMAIDETGAITTDPVKAMLGALLPFGGYKGANIALIV
EMLSAGLSGAAWSLDAGHFLSGEHPVNAGMTVIALFPMAADSDFPERAKAQLERLRSKGVRIPGDRAMPASVTETDSLEI
DIGVLETIRRLRLQ
>Mature_333_residues
AGQRIRMPIGELRHLALEACLACGGSPAMAKALVDATLSAACFGRTELGFPHFVDYLTSLRDGRINGDAKPRFNHVLPAL
IQADADGGIAQLGFDLIYDDFTTRVKTFGISVFTQRNSYTAGELGYYVRRLAQDGLISIAAANGPALMAAAEGGERVYCT
NPLAFGLPLPEPLPPLVIDQATSATAFVTLAEAAKEQSPIAHGMAIDETGAITTDPVKAMLGALLPFGGYKGANIALIVE
MLSAGLSGAAWSLDAGHFLSGEHPVNAGMTVIALFPMAADSDFPERAKAQLERLRSKGVRIPGDRAMPASVTETDSLEID
IGVLETIRRLRLQ

Specific function: Unknown

COG id: COG2055

COG function: function code C; Malate/L-lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH2/MDH2 oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787020, Length=230, Percent_Identity=27.3913043478261, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790000, Length=293, Percent_Identity=25.5972696245734, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786727, Length=193, Percent_Identity=27.4611398963731, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17536633, Length=293, Percent_Identity=21.160409556314, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24667908, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24667904, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45553211, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24667912, Length=313, Percent_Identity=26.1980830670927, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24658010, Length=305, Percent_Identity=24.5901639344262, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003767 [H]

Pfam domain/function: PF02615 Ldh_2 [H]

EC number: 1.1.1.37

Molecular weight: Translated: 35280; Mature: 35149

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQRIRMPIGELRHLALEACLACGGSPAMAKALVDATLSAACFGRTELGFPHFVDYLTS
CCCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LRDGRINGDAKPRFNHVLPALIQADADGGIAQLGFDLIYDDFTTRVKTFGISVFTQRNSY
HCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCC
TAGELGYYVRRLAQDGLISIAAANGPALMAAAEGGERVYCTNPLAFGLPLPEPLPPLVID
CHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCEEEECCCEEECCCCCCCCCCCEEE
QATSATAFVTLAEAAKEQSPIAHGMAIDETGAITTDPVKAMLGALLPFGGYKGANIALIV
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHH
EMLSAGLSGAAWSLDAGHFLSGEHPVNAGMTVIALFPMAADSDFPERAKAQLERLRSKGV
HHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
RIPGDRAMPASVTETDSLEIDIGVLETIRRLRLQ
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AGQRIRMPIGELRHLALEACLACGGSPAMAKALVDATLSAACFGRTELGFPHFVDYLTS
CCCEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
LRDGRINGDAKPRFNHVLPALIQADADGGIAQLGFDLIYDDFTTRVKTFGISVFTQRNSY
HCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCC
TAGELGYYVRRLAQDGLISIAAANGPALMAAAEGGERVYCTNPLAFGLPLPEPLPPLVID
CHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCCCCEEEECCCEEECCCCCCCCCCCEEE
QATSATAFVTLAEAAKEQSPIAHGMAIDETGAITTDPVKAMLGALLPFGGYKGANIALIV
CCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHH
EMLSAGLSGAAWSLDAGHFLSGEHPVNAGMTVIALFPMAADSDFPERAKAQLERLRSKGV
HHHHCCCCCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCC
RIPGDRAMPASVTETDSLEIDIGVLETIRRLRLQ
CCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-malate; NAD+

Specific reaction: (S)-malate + NAD+ = oxaloacetate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]