BacMap

Definition	Rhizobium leguminosarum bv. viciae 3841 plasmid pRL12, complete sequence.
Accession	NC_008378
Length	870,021

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The map label for this gene is livG [H]

Identifier: 116249156

GI number: 116249156

Start: 534602

End: 535357

Strand: Reverse

Name: livG [H]

Synonym: pRL120492

Alternate gene names: 116249156

Gene position: 535357-534602 (Counterclockwise)

Preceding gene: 116249157

Following gene: 116249155

Centisome position: 61.53

GC content: 65.61

Gene sequence:

>756_bases
GTGCTGCAGCGGGAAGCTCAAAGACGGCCGGGGGGAGCCTTTCTCTCGGCCAAGGGGATCCACAAGCGGTTCGGCGCGCT
GGTGGTGCTGGAGAATCTTGATTTTTCCATGGGCGATGGCGAAGCGATCGGCATTGTCGGGCCGAACGGCGCGGGCAAGA
CGACCCTGCTCAGCGTGCTCGCCGGCGCCTTCCCGCCGAATGAAGGAAGCATCACCTTCGACGGCGTCGACGTCACGCGC
CGGACGGCGGCGGAACGCTGCCGCTCCGGGCTCGTCCGGACCCATCAGATTCCCAAGCCCTTCGGCGGCATGACGACCTT
CGAAAATGTCTTCGTCGCCGCGTCGCACGGCAATGCCGCAAGCCGCGACGAGGCCTATGAGCGCGTGGTCGATTCCCTTT
CGCTCTGCGGCATGCTCGCCGTCGCCAACCGCCCCGCCGACACGCTCGGCCTGCTGGACCGCAAACGCCTGGAGCTTGCC
CGTGCGCTTGCCACGCAGCCGAGGCTGATGCTGCTCGACGAGATCGGCGGCGGCCTGACCGATGGCGAGGCGAGCGAACT
GGTGGAAACCATCCTGGAACTGCGCCGCCGCGGCATCGGCATCGTCTGGATCGAACATATCGTTCATATCCTCCTGCAGG
TGGCGGAGCGGCTGATCTGCATGGACGCCGGCAGGATCATCGCCGATGGCGAACCGAAAATGGTCATGAGCAATGCGGAG
GTGGTCAAGGCCTATCTCGGAGGAACGCCCGCATGA

Upstream 100 bases:

>100_bases
GGGCTCGAAATTCGCGCTCGACTACGTCGTCACCGAAAACGCCACCGACCCCAACGTCCCGGTCGGCGCCAAGCTCACCG
CCTATAACGGGTAACGCACA

Downstream 100 bases:

>100_bases
GCCTTCTCTCCATCCGCGACCTCGACGTCCGCCACGGCCTGCTCCAGGCGGTGCGCGGGGTCAGTTTCGATCTTGCCAGG
GGCGAGGTGCTGGCGCTGGT

Product: putative ATP-binding component of ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein G [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLQREAQRRPGGAFLSAKGIHKRFGALVVLENLDFSMGDGEAIGIVGPNGAGKTTLLSVLAGAFPPNEGSITFDGVDVTR
RTAAERCRSGLVRTHQIPKPFGGMTTFENVFVAASHGNAASRDEAYERVVDSLSLCGMLAVANRPADTLGLLDRKRLELA
RALATQPRLMLLDEIGGGLTDGEASELVETILELRRRGIGIVWIEHIVHILLQVAERLICMDAGRIIADGEPKMVMSNAE
VVKAYLGGTPA

Sequences:

>Translated_251_residues
MLQREAQRRPGGAFLSAKGIHKRFGALVVLENLDFSMGDGEAIGIVGPNGAGKTTLLSVLAGAFPPNEGSITFDGVDVTR
RTAAERCRSGLVRTHQIPKPFGGMTTFENVFVAASHGNAASRDEAYERVVDSLSLCGMLAVANRPADTLGLLDRKRLELA
RALATQPRLMLLDEIGGGLTDGEASELVETILELRRRGIGIVWIEHIVHILLQVAERLICMDAGRIIADGEPKMVMSNAE
VVKAYLGGTPA
>Mature_251_residues
MLQREAQRRPGGAFLSAKGIHKRFGALVVLENLDFSMGDGEAIGIVGPNGAGKTTLLSVLAGAFPPNEGSITFDGVDVTR
RTAAERCRSGLVRTHQIPKPFGGMTTFENVFVAASHGNAASRDEAYERVVDSLSLCGMLAVANRPADTLGLLDRKRLELA
RALATQPRLMLLDEIGGGLTDGEASELVETILELRRRGIGIVWIEHIVHILLQVAERLICMDAGRIIADGEPKMVMSNAE
VVKAYLGGTPA

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI171184400, Length=235, Percent_Identity=26.3829787234043, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27881501, Length=162, Percent_Identity=28.3950617283951, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI30795238, Length=162, Percent_Identity=28.3950617283951, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI116734710, Length=199, Percent_Identity=26.6331658291457, Blast_Score=66, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=249, Percent_Identity=33.7349397590361, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1787029, Length=224, Percent_Identity=31.25, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787792, Length=238, Percent_Identity=28.5714285714286, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1789593, Length=235, Percent_Identity=28.936170212766, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787370, Length=219, Percent_Identity=31.5068493150685, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082268, Length=236, Percent_Identity=30.9322033898305, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI87081709, Length=221, Percent_Identity=28.5067873303167, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789991, Length=197, Percent_Identity=30.9644670050761, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI48995001, Length=236, Percent_Identity=24.5762711864407, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789672, Length=242, Percent_Identity=24.7933884297521, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788761, Length=222, Percent_Identity=31.0810810810811, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788472, Length=227, Percent_Identity=26.431718061674, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87081791, Length=232, Percent_Identity=27.5862068965517, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788225, Length=233, Percent_Identity=28.3261802575107, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1786872, Length=222, Percent_Identity=30.1801801801802, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786803, Length=229, Percent_Identity=28.82096069869, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790190, Length=222, Percent_Identity=26.5765765765766, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790525, Length=240, Percent_Identity=25.4166666666667, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788210, Length=236, Percent_Identity=25, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786345, Length=227, Percent_Identity=28.6343612334802, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081782, Length=225, Percent_Identity=26.2222222222222, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081834, Length=208, Percent_Identity=31.7307692307692, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786698, Length=179, Percent_Identity=28.4916201117318, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789032, Length=244, Percent_Identity=25.8196721311475, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786398, Length=227, Percent_Identity=26.431718061674, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788165, Length=237, Percent_Identity=25.7383966244726, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787089, Length=239, Percent_Identity=25.9414225941423, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788897, Length=222, Percent_Identity=25.2252252252252, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786253, Length=218, Percent_Identity=28.8990825688073, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789873, Length=208, Percent_Identity=27.4038461538462, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790544, Length=227, Percent_Identity=26.8722466960352, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI48994943, Length=244, Percent_Identity=27.0491803278689, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788644, Length=251, Percent_Identity=27.0916334661355, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786563, Length=214, Percent_Identity=27.1028037383178, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786319, Length=207, Percent_Identity=27.0531400966184, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790467, Length=226, Percent_Identity=23.8938053097345, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787712, Length=221, Percent_Identity=25.3393665158371, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790739, Length=230, Percent_Identity=26.5217391304348, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI48994883, Length=239, Percent_Identity=27.6150627615063, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788450, Length=232, Percent_Identity=24.5689655172414, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786654, Length=221, Percent_Identity=23.9819004524887, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1789586, Length=229, Percent_Identity=27.5109170305677, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1789891, Length=206, Percent_Identity=25.7281553398058, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI87082267, Length=245, Percent_Identity=26.9387755102041, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI193202349, Length=238, Percent_Identity=26.0504201680672, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17565938, Length=220, Percent_Identity=26.3636363636364, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=203, Percent_Identity=29.064039408867, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115534520, Length=210, Percent_Identity=26.1904761904762, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17567265, Length=220, Percent_Identity=28.1818181818182, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI212646699, Length=220, Percent_Identity=24.5454545454545, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=215, Percent_Identity=26.5116279069767, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17567269, Length=219, Percent_Identity=26.9406392694064, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17569081, Length=240, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115535101, Length=224, Percent_Identity=27.2321428571429, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=224, Percent_Identity=25, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=213, Percent_Identity=27.6995305164319, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=212, Percent_Identity=26.4150943396226, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322980, Length=234, Percent_Identity=25.2136752136752, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24659289, Length=218, Percent_Identity=26.1467889908257, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24666092, Length=236, Percent_Identity=27.9661016949153, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24643674, Length=218, Percent_Identity=26.1467889908257, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648314, Length=216, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI85726357, Length=221, Percent_Identity=26.6968325791855, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574533, Length=214, Percent_Identity=28.5046728971963, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26825; Mature: 26825

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQREAQRRPGGAFLSAKGIHKRFGALVVLENLDFSMGDGEAIGIVGPNGAGKTTLLSVL
CCCCCHHCCCCCCEEEHHHHHHHHCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHH
AGAFPPNEGSITFDGVDVTRRTAAERCRSGLVRTHQIPKPFGGMTTFENVFVAASHGNAA
HHCCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHEEEEECCCCCC
SRDEAYERVVDSLSLCGMLAVANRPADTLGLLDRKRLELARALATQPRLMLLDEIGGGLT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCC
DGEASELVETILELRRRGIGIVWIEHIVHILLQVAERLICMDAGRIIADGEPKMVMSNAE
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECHHH
VVKAYLGGTPA
HHHHHHCCCCC
>Mature Secondary Structure
MLQREAQRRPGGAFLSAKGIHKRFGALVVLENLDFSMGDGEAIGIVGPNGAGKTTLLSVL
CCCCCHHCCCCCCEEEHHHHHHHHCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHH
AGAFPPNEGSITFDGVDVTRRTAAERCRSGLVRTHQIPKPFGGMTTFENVFVAASHGNAA
HHCCCCCCCCEEECCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCHHHHHEEEEECCCCCC
SRDEAYERVVDSLSLCGMLAVANRPADTLGLLDRKRLELARALATQPRLMLLDEIGGGLT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCC
DGEASELVETILELRRRGIGIVWIEHIVHILLQVAERLICMDAGRIIADGEPKMVMSNAE
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECHHH
VVKAYLGGTPA
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]