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The map label for this gene is yhxC [H]

Identifier: 116249152

GI number: 116249152

Start: 531112

End: 531915

Strand: Reverse

Name: yhxC [H]

Synonym: pRL120488

Alternate gene names: 116249152

Gene position: 531915-531112 (Counterclockwise)

Preceding gene: 116249153

Following gene: 116249151

Centisome position: 61.14

GC content: 66.79

Gene sequence:

>804_bases
TTGCGAAAGGTGACGTTCATGCTTTTTGGTAAAACGATCCTGGTGACCGGCGTCGCTTCCGGCATCGGCGCCCGCACGGC
GGAGCTGGCCGGCCAGATGGGTGCCGAGGTCATCGGCGTCGATGTGCGCGAACCGGTGGGTGGAAGCGCTGCCTTCATCA
AGGGCGATCTTTCGACCGCCGCTGGCGTTGCCGAGATCGTCGCGCAGCTTCCGGCGCGCCTCGACGCACTCGCCAATGTC
GCCGGCCTTTCCGGCAGCACCGGGGTCGTCTCGACACTCGCCGTCAATTTCTACGGGCTTCGCGCCCTGTCCGAAGCCGT
GGCGCCCCGCCTGCGCGAAGGTGGGGCCATCGTCAACGTCGCCTCGATCGCCGGCTACGGCTGGCGCGCCAATCTCGAGC
GCGCGAAGTCGCTCACCGCCATTGAAGGCTTTCCCGAGGTGGCGGCGGTCGTTACTGAGCACGGCCTCAAGGACGAGGAA
GGTTATCCGCTCTCCAAGGAGCTGCTGCTGCTCTGGACGATGCGGGCGGCCCATCAGCCGCTGTTCAAAAACCGCGGCAT
CCGGGTGAATGCGGTCAGCCCCGGGCCGGTGGAAACGCCGATTCTCAAGCAGTTCCGCGCCGTGCTCGGCGACGCCAGGG
TCGATAGCGACATTACCCGCGTCGGCCGCGCCGGCACCTCCGCCGATATTGCGCCCGCCGTGCTCTTCCTCTGCTCGGAT
GGCGCACGCTGGATCAACGGCGCCAATCTTGCCGCCGACGGCGGCCTCGAAGCTTCCATCAACGCCGAAGTCCTCGGCTT
CTAG

Upstream 100 bases:

>100_bases
CGCTTCGACTTTCAACTTCTGCCGGTCGGCTATCGGCTGAAATTGCCCGGCCCGCATAAAATCAAATGGGAAGAATGAGC
CGGCTTATTTCCGGACTCTC

Downstream 100 bases:

>100_bases
TTCTTGTCCCAAGGAGACCTCCGATGAATATTTCCCTGCTTATCAACGGCGCAGACCGCGCGGCCTCCGGCGGCCGGACC
TATGATCGCATCGATCCCTT

Product: 3-alpha-hydroxysteroid dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORFX [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRKVTFMLFGKTILVTGVASGIGARTAELAGQMGAEVIGVDVREPVGGSAAFIKGDLSTAAGVAEIVAQLPARLDALANV
AGLSGSTGVVSTLAVNFYGLRALSEAVAPRLREGGAIVNVASIAGYGWRANLERAKSLTAIEGFPEVAAVVTEHGLKDEE
GYPLSKELLLLWTMRAAHQPLFKNRGIRVNAVSPGPVETPILKQFRAVLGDARVDSDITRVGRAGTSADIAPAVLFLCSD
GARWINGANLAADGGLEASINAEVLGF

Sequences:

>Translated_267_residues
MRKVTFMLFGKTILVTGVASGIGARTAELAGQMGAEVIGVDVREPVGGSAAFIKGDLSTAAGVAEIVAQLPARLDALANV
AGLSGSTGVVSTLAVNFYGLRALSEAVAPRLREGGAIVNVASIAGYGWRANLERAKSLTAIEGFPEVAAVVTEHGLKDEE
GYPLSKELLLLWTMRAAHQPLFKNRGIRVNAVSPGPVETPILKQFRAVLGDARVDSDITRVGRAGTSADIAPAVLFLCSD
GARWINGANLAADGGLEASINAEVLGF
>Mature_267_residues
MRKVTFMLFGKTILVTGVASGIGARTAELAGQMGAEVIGVDVREPVGGSAAFIKGDLSTAAGVAEIVAQLPARLDALANV
AGLSGSTGVVSTLAVNFYGLRALSEAVAPRLREGGAIVNVASIAGYGWRANLERAKSLTAIEGFPEVAAVVTEHGLKDEE
GYPLSKELLLLWTMRAAHQPLFKNRGIRVNAVSPGPVETPILKQFRAVLGDARVDSDITRVGRAGTSADIAPAVLFLCSD
GARWINGANLAADGGLEASINAEVLGF

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Drosophila melanogaster, GI28571526, Length=279, Percent_Identity=27.9569892473118, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21357041, Length=279, Percent_Identity=28.3154121863799, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24639444, Length=279, Percent_Identity=28.3154121863799, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27562; Mature: 27562

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVTFMLFGKTILVTGVASGIGARTAELAGQMGAEVIGVDVREPVGGSAAFIKGDLSTA
CCEEEEEEECCHHEEHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHH
AGVAEIVAQLPARLDALANVAGLSGSTGVVSTLAVNFYGLRALSEAVAPRLREGGAIVNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ASIAGYGWRANLERAKSLTAIEGFPEVAAVVTEHGLKDEEGYPLSKELLLLWTMRAAHQP
HHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
LFKNRGIRVNAVSPGPVETPILKQFRAVLGDARVDSDITRVGRAGTSADIAPAVLFLCSD
HHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCC
GARWINGANLAADGGLEASINAEVLGF
CCCEECCCCEECCCCCCEECCCEEECC
>Mature Secondary Structure
MRKVTFMLFGKTILVTGVASGIGARTAELAGQMGAEVIGVDVREPVGGSAAFIKGDLSTA
CCEEEEEEECCHHEEHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHHH
AGVAEIVAQLPARLDALANVAGLSGSTGVVSTLAVNFYGLRALSEAVAPRLREGGAIVNV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
ASIAGYGWRANLERAKSLTAIEGFPEVAAVVTEHGLKDEEGYPLSKELLLLWTMRAAHQP
HHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
LFKNRGIRVNAVSPGPVETPILKQFRAVLGDARVDSDITRVGRAGTSADIAPAVLFLCSD
HHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHCCCCCCCHHHHHHHHHCCC
GARWINGANLAADGGLEASINAEVLGF
CCCEECCCCEECCCCCCEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8196543 [H]