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The map label for this gene is pcaQ [H]

Identifier: 116248944

GI number: 116248944

Start: 289957

End: 291207

Strand: Direct

Name: pcaQ [H]

Synonym: pRL120275

Alternate gene names: 116248944

Gene position: 289957-291207 (Clockwise)

Preceding gene: 116248938

Following gene: 116248945

Centisome position: 33.33

GC content: 64.91

Gene sequence:

>1251_bases
ATGATTGCACACCGCGAACTCAACCTTCGCCACCTCGAGGCCGTCGCTGCCGCCGCGCGGCTGGGCAGCATCAGCAGCGC
GTCTCACACCATGAATTTGTCGCAGCCTGCTCTGACCCAGGCCGTTGCCAAGGTCGAGGCGCAGCTTGGCCATGTGCTGT
TCGATCGCCAGCCGAGCGGCGTTACCAGCACCGAGGCGGGGCGCTTGATCACGGCCCGCATCGAACGCGCACTCGCCTAT
GTGGCGCGCGGCGGCCAAAGCGTGCGGCGGGGCGCTCGCTTGCCGCCGCTGCCCCATATCGAGCGGCGCATCACTTTTGG
ACAATTGCGTGCGCTAATCGCGGTGGATCAAGCAGGCAGCTTTGCGCTGGCATCGAAGCGCATTGGCCTGTCTGAACCGG
CATTGCATCGGGCGAGCCGCGATCTGGAGCAGTTGCTGGGTGTGCCGCTTCTCGTCCGGCAGGGGCGTACCGTTCAACCG
ACGGCCGCCGCCGCCGTGTTGCTGCGGTTCGCCCGTCTGGCGCAATCGGAGCTAGAGGCTGGGTTCGACGAATTGGAGGC
ACTGCGGTCGGAGGGCGCCGGACGTGTCACTGTCGGCACCATGCCGCTGGCGCGCGCAATCCTGTTGCCGCAGGCATTGG
CTCGTTTCGCTCGCGCCTATCCAATGGCGTCGGTGAACGTGGTGGAGGGGCCATATGTGGAGCTGTTGGCCAGGCTCCGC
GAAGGCGAGATGGATCTGCTGATCGGGGCAATGCGCGACCCCCCGCCGGTCAAGGACATCGCTCAGGAACCGCTGTTCAT
CGATGATCCGGTGATCGTCGGTCGTGCCGGACACCCCCTGCTGTCCAAGCCGGGATTTGCCTTCTCGCGGCTATTGGATT
TCCCATGGGTTATCGCCGCGACGGGCGCGCCGGTGCGCCATCGGTGGGAGGAGATGTTCACAGAGCACGGGCTTGAGCCG
CCACGGCTACGCATCGAATGCGGTTCGGTTCTGGTGGTCCGCGGGCTGATGCTGGAGGACGATTGGTTGACGCTGATGTC
GCGCGACCAGTTCCTGTTCGAGCGGCGTGCTGGCCTGCTTGGCGAAATCGCCGGCGCTGGGCTGTCCTTGCGTCGTCAGA
TCGGCCTCACGGTGCGCGATGACTGGCGTCCGACACAGCTGCAAACGGCATTCACGGATACCTTTCGCACGGTTTGCGCG
CAGTGGACTTCAGGAAAAGCAATGGAGCGTGAACCCTTCCGATATGCCTGA

Upstream 100 bases:

>100_bases
GATCCTCCCTCCTGTCCTGGCTGGGATTTAAGGAGGGAATATTGAGCCGCAAAGCAAACTTGATTTGCGCACCGGTCATA
AGCTGTGCTGAAGTGTGACG

Downstream 100 bases:

>100_bases
TCGTCGTCCGGTTCGTTAGAACGGGGCATGAGCAAACACCAAACGATCGCATTCACCGGTTTTGGAAAGCCTTCGCAGGC
TTTCTGTCGGGGCTGGCGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pca operon transcriptional activator [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MIAHRELNLRHLEAVAAAARLGSISSASHTMNLSQPALTQAVAKVEAQLGHVLFDRQPSGVTSTEAGRLITARIERALAY
VARGGQSVRRGARLPPLPHIERRITFGQLRALIAVDQAGSFALASKRIGLSEPALHRASRDLEQLLGVPLLVRQGRTVQP
TAAAAVLLRFARLAQSELEAGFDELEALRSEGAGRVTVGTMPLARAILLPQALARFARAYPMASVNVVEGPYVELLARLR
EGEMDLLIGAMRDPPPVKDIAQEPLFIDDPVIVGRAGHPLLSKPGFAFSRLLDFPWVIAATGAPVRHRWEEMFTEHGLEP
PRLRIECGSVLVVRGLMLEDDWLTLMSRDQFLFERRAGLLGEIAGAGLSLRRQIGLTVRDDWRPTQLQTAFTDTFRTVCA
QWTSGKAMEREPFRYA

Sequences:

>Translated_416_residues
MIAHRELNLRHLEAVAAAARLGSISSASHTMNLSQPALTQAVAKVEAQLGHVLFDRQPSGVTSTEAGRLITARIERALAY
VARGGQSVRRGARLPPLPHIERRITFGQLRALIAVDQAGSFALASKRIGLSEPALHRASRDLEQLLGVPLLVRQGRTVQP
TAAAAVLLRFARLAQSELEAGFDELEALRSEGAGRVTVGTMPLARAILLPQALARFARAYPMASVNVVEGPYVELLARLR
EGEMDLLIGAMRDPPPVKDIAQEPLFIDDPVIVGRAGHPLLSKPGFAFSRLLDFPWVIAATGAPVRHRWEEMFTEHGLEP
PRLRIECGSVLVVRGLMLEDDWLTLMSRDQFLFERRAGLLGEIAGAGLSLRRQIGLTVRDDWRPTQLQTAFTDTFRTVCA
QWTSGKAMEREPFRYA
>Mature_416_residues
MIAHRELNLRHLEAVAAAARLGSISSASHTMNLSQPALTQAVAKVEAQLGHVLFDRQPSGVTSTEAGRLITARIERALAY
VARGGQSVRRGARLPPLPHIERRITFGQLRALIAVDQAGSFALASKRIGLSEPALHRASRDLEQLLGVPLLVRQGRTVQP
TAAAAVLLRFARLAQSELEAGFDELEALRSEGAGRVTVGTMPLARAILLPQALARFARAYPMASVNVVEGPYVELLARLR
EGEMDLLIGAMRDPPPVKDIAQEPLFIDDPVIVGRAGHPLLSKPGFAFSRLLDFPWVIAATGAPVRHRWEEMFTEHGLEP
PRLRIECGSVLVVRGLMLEDDWLTLMSRDQFLFERRAGLLGEIAGAGLSLRRQIGLTVRDDWRPTQLQTAFTDTFRTVCA
QWTSGKAMEREPFRYA

Specific function: Activates transcription of the pcaDCHGB operon for the catabolism of the phenolic compound protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787601, Length=302, Percent_Identity=23.841059602649, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081904, Length=244, Percent_Identity=29.9180327868852, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789506, Length=253, Percent_Identity=24.5059288537549, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788887, Length=270, Percent_Identity=28.1481481481481, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI157672245, Length=239, Percent_Identity=27.6150627615063, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=168, Percent_Identity=30.952380952381, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=204, Percent_Identity=29.9019607843137, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=24.8299319727891, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR012787
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 45691; Mature: 45691

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAHRELNLRHLEAVAAAARLGSISSASHTMNLSQPALTQAVAKVEAQLGHVLFDRQPSG
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
VTSTEAGRLITARIERALAYVARGGQSVRRGARLPPLPHIERRITFGQLRALIAVDQAGS
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
FALASKRIGLSEPALHRASRDLEQLLGVPLLVRQGRTVQPTAAAAVLLRFARLAQSELEA
CHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GFDELEALRSEGAGRVTVGTMPLARAILLPQALARFARAYPMASVNVVEGPYVELLARLR
CHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
EGEMDLLIGAMRDPPPVKDIAQEPLFIDDPVIVGRAGHPLLSKPGFAFSRLLDFPWVIAA
CCCHHEEEECCCCCCCHHHHHCCCCEECCCEEECCCCCCCCCCCCHHHHHHHCCCEEEEE
TGAPVRHRWEEMFTEHGLEPPRLRIECGSVLVVRGLMLEDDWLTLMSRDQFLFERRAGLL
CCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GEIAGAGLSLRRQIGLTVRDDWRPTQLQTAFTDTFRTVCAQWTSGKAMEREPFRYA
HHHHCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIAHRELNLRHLEAVAAAARLGSISSASHTMNLSQPALTQAVAKVEAQLGHVLFDRQPSG
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
VTSTEAGRLITARIERALAYVARGGQSVRRGARLPPLPHIERRITFGQLRALIAVDQAGS
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
FALASKRIGLSEPALHRASRDLEQLLGVPLLVRQGRTVQPTAAAAVLLRFARLAQSELEA
CHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GFDELEALRSEGAGRVTVGTMPLARAILLPQALARFARAYPMASVNVVEGPYVELLARLR
CHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHH
EGEMDLLIGAMRDPPPVKDIAQEPLFIDDPVIVGRAGHPLLSKPGFAFSRLLDFPWVIAA
CCCHHEEEECCCCCCCHHHHHCCCCEECCCEEECCCCCCCCCCCCHHHHHHHCCCEEEEE
TGAPVRHRWEEMFTEHGLEPPRLRIECGSVLVVRGLMLEDDWLTLMSRDQFLFERRAGLL
CCCHHHHHHHHHHHHCCCCCCEEEEECCCEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHH
GEIAGAGLSLRRQIGLTVRDDWRPTQLQTAFTDTFRTVCAQWTSGKAMEREPFRYA
HHHHCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]