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The map label for this gene is tpiA [H]

Identifier: 116248879

GI number: 116248879

Start: 219214

End: 219984

Strand: Direct

Name: tpiA [H]

Synonym: pRL120209

Alternate gene names: 116248879

Gene position: 219214-219984 (Clockwise)

Preceding gene: 116248878

Following gene: 116248880

Centisome position: 25.2

GC content: 62.13

Gene sequence:

>771_bases
ATGACCGAAAAGCCGCGCTTCTGGATTGGCACCAGCTGGAAGATGAACAAGACGCTTACTGAAGCCGAGCAATTCGCGCG
CGGTCTCGAAGCCGCCGATGGCGCGCGTGACCCGCGCATCCATCGCTTCGTGATTCCACCCTTCACGGCCGTGCGCGAGG
TCAAGGCGATGCTTGCCAAGACCTCGGTGAAGGTCGGCGCGCAGAACATGCATTGGGCCGACCAGGGCGCCTGGACCGGC
GAAGTCTCGCCGGTGATGCTGAAGGACTGCAATCTCGATCTCGTCGAGCTCGGCCATTCCGAACGGCGCGAACATTTTGG
AGAGACCGACGAGACAGTGGGTCTGAAGACCGAAGCAGCCGTTCGCCACGGCCTCATTCCGCTGATCTGCATTGGCGAGA
CGCTTTCCGACCGCGAAAGCGGAAAGGCCGCCGACATCCTCGCGACCCAGGTGCGCGGCGCGCTATCGAAACTTTCGGGC
GCCCAGAAAAGCGCCGAAATCCTGCTCGCCTACGAGCCCGTCTGGGCGATTGGCGAGAAGGGCATCCCGGCGACTGCGGA
TTATGCCGATGCCCGCCAGTCTGAAATCATCGCCGTTGCCGAAGCTGTGCTGGGCCGGAAGATCCCCTGCCTCTATGGCG
GGTCGGTCAACCCGCAGAACTGCGAAGAACTGATCTCAAGCCCGCATATCGACGGGCTCTTCATCGGCCGTTCGGCCTGG
AATGTCGAAGGCTACCTCGACATCCTGGCCAGATGCGCCGCCAAACTTTAA

Upstream 100 bases:

>100_bases
TCGACACCGTTATCACCGACAGCGCACCGGATGCTGCTGTTCTGGAGGAATTCGAGCAGGCGGGCATTACGCTCACCATT
GCATCAACGCAGGATCCGAC

Downstream 100 bases:

>100_bases
GGAGAAAGACAATGAAGCTTGCCATTGCAGGAGACAGCGCCGGCGAAGGCCTCGCCAAGGTTCTGGCCGACCATCTGAAA
GAGCGCTTCGACGTTTACGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2 [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTEKPRFWIGTSWKMNKTLTEAEQFARGLEAADGARDPRIHRFVIPPFTAVREVKAMLAKTSVKVGAQNMHWADQGAWTG
EVSPVMLKDCNLDLVELGHSERREHFGETDETVGLKTEAAVRHGLIPLICIGETLSDRESGKAADILATQVRGALSKLSG
AQKSAEILLAYEPVWAIGEKGIPATADYADARQSEIIAVAEAVLGRKIPCLYGGSVNPQNCEELISSPHIDGLFIGRSAW
NVEGYLDILARCAAKL

Sequences:

>Translated_256_residues
MTEKPRFWIGTSWKMNKTLTEAEQFARGLEAADGARDPRIHRFVIPPFTAVREVKAMLAKTSVKVGAQNMHWADQGAWTG
EVSPVMLKDCNLDLVELGHSERREHFGETDETVGLKTEAAVRHGLIPLICIGETLSDRESGKAADILATQVRGALSKLSG
AQKSAEILLAYEPVWAIGEKGIPATADYADARQSEIIAVAEAVLGRKIPCLYGGSVNPQNCEELISSPHIDGLFIGRSAW
NVEGYLDILARCAAKL
>Mature_255_residues
TEKPRFWIGTSWKMNKTLTEAEQFARGLEAADGARDPRIHRFVIPPFTAVREVKAMLAKTSVKVGAQNMHWADQGAWTGE
VSPVMLKDCNLDLVELGHSERREHFGETDETVGLKTEAAVRHGLIPLICIGETLSDRESGKAADILATQVRGALSKLSGA
QKSAEILLAYEPVWAIGEKGIPATADYADARQSEIIAVAEAVLGRKIPCLYGGSVNPQNCEELISSPHIDGLFIGRSAWN
VEGYLDILARCAAKL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=254, Percent_Identity=35.8267716535433, Blast_Score=138, Evalue=6e-33,
Organism=Homo sapiens, GI226529917, Length=254, Percent_Identity=35.8267716535433, Blast_Score=137, Evalue=6e-33,
Organism=Escherichia coli, GI1790353, Length=208, Percent_Identity=35.5769230769231, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17536593, Length=240, Percent_Identity=36.6666666666667, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=32.3886639676113, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI28572008, Length=248, Percent_Identity=35.4838709677419, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI28572006, Length=248, Percent_Identity=35.4838709677419, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI28572004, Length=248, Percent_Identity=35.4838709677419, Blast_Score=136, Evalue=1e-32,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27850; Mature: 27719

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKPRFWIGTSWKMNKTLTEAEQFARGLEAADGARDPRIHRFVIPPFTAVREVKAMLAK
CCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHH
TSVKVGAQNMHWADQGAWTGEVSPVMLKDCNLDLVELGHSERREHFGETDETVGLKTEAA
HHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHH
VRHGLIPLICIGETLSDRESGKAADILATQVRGALSKLSGAQKSAEILLAYEPVWAIGEK
HHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCC
GIPATADYADARQSEIIAVAEAVLGRKIPCLYGGSVNPQNCEELISSPHIDGLFIGRSAW
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCEEEECCCCC
NVEGYLDILARCAAKL
CHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEKPRFWIGTSWKMNKTLTEAEQFARGLEAADGARDPRIHRFVIPPFTAVREVKAMLAK
CCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHH
TSVKVGAQNMHWADQGAWTGEVSPVMLKDCNLDLVELGHSERREHFGETDETVGLKTEAA
HHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCHHHHHHCCCCCCCCCCCHHHH
VRHGLIPLICIGETLSDRESGKAADILATQVRGALSKLSGAQKSAEILLAYEPVWAIGEK
HHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCC
GIPATADYADARQSEIIAVAEAVLGRKIPCLYGGSVNPQNCEELISSPHIDGLFIGRSAW
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCEEEECCCCC
NVEGYLDILARCAAKL
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708387 [H]