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The map label for this gene is cbf5

Identifier: 11497854

GI number: 11497854

Start: 213248

End: 214225

Strand: Reverse

Name: cbf5

Synonym: AF0238

Alternate gene names: 11497854

Gene position: 214225-213248 (Counterclockwise)

Preceding gene: 11497857

Following gene: 11497851

Centisome position: 9.83

GC content: 50.72

Gene sequence:

>978_bases
GTGAAACTGGAAAACTTCTACGTCAAGGATGACGCAAGCACGGATGAGAGCTACGGATGCTACCCAACCAAGAGGCCGAT
GGAGGAGTACATCAGGAAGGGGTTAGTGTGCATCGACAAGCCCATGGGGCCAAGCAGCCACGAGGTGGTTGTTTGGGTGA
GGAGGATTCTCAATGTCAGCAAAACGGGCCACGCAGGCACTCTCGACCCAAGAGTTACAGGCGTTCTGCCAATTTTCATT
GAAAATGCAACAAAGATGGTCAAGTTCCTTCAGGAGTCGAGCAAGGAGTACGTCTGCCTCATGAGGCTTCACGGTGATGC
GAAGAGGGAGGATGTTGAGAAGGTGATGAAGCTCTTCGTCGGCAGAATCTACCAGCGCCCCCCGCTTAAGTCCGCGGTTA
AAAAAGTGCTGAGAATCAGGGAGATTTACGAAATGGAGCTTCTGGAGATGGAGGGCAGGGACGTGCTTTTCAGAGTTGTT
ACTGAATCTGGAACATACATCAGAAAGCTCTGCAGGGACATCGGAGAGGTGCTTGGCACTGGGGCGCACATGCAGGAGCT
AAGAAGAACTCGGACGGGGAAATTTGGAGAGGACATGTGCTACACACTGCAGGACCTGCTTGATGCTTACGTTTTCTGGA
AGGAGGAGGGAGAAGAGAAGTATTTAAGGGAGATAATCAAGCCGATGGAGGTTGCTGCTGCAGAGCTGCCCAAAATCGTC
ATCAAAGATTCCGCTGTTGATGCCATCTGCCATGGGGCGAATCTTTCAGTGAGGGGAGTGGCTTACGTGGAGAAGAATGT
TAAGAAGGACAGCACTGTGGCGATCTTCACGCTGAAAAACGAGCTGGTGGCAATTGGCAGAGCTTTAATGGATGCAGAAG
ACATCTACAGGCTTAAAAAAGGAATTGCTGCGGACATTCAGAGAGTGATGATGGAGAGGGGAGTGTATCCCAAAGTCTGG
AAGTCCTCTTCTGATTAG

Upstream 100 bases:

>100_bases
ACTTCACGCTGGCTTTACAAAAAAAGCGTTTAAAATGTTTTCGATGCTGACACCTCAAAGCTGGCTAAAAAGTTAAAAAG
TTCGAGGGATTAGGGAGGAT

Downstream 100 bases:

>100_bases
GGCTGTTTTTCGCTGGATGATAGGGCGAGGAACACGGCAAAGGAGATTATGAAGGTCCAGACCGAAGCTGGAATGCTGAG
GGGCGAGAGCTTCTGGAAGC

Product: H/ACA RNA-protein complex component Cbf5p

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKLENFYVKDDASTDESYGCYPTKRPMEEYIRKGLVCIDKPMGPSSHEVVVWVRRILNVSKTGHAGTLDPRVTGVLPIFI
ENATKMVKFLQESSKEYVCLMRLHGDAKREDVEKVMKLFVGRIYQRPPLKSAVKKVLRIREIYEMELLEMEGRDVLFRVV
TESGTYIRKLCRDIGEVLGTGAHMQELRRTRTGKFGEDMCYTLQDLLDAYVFWKEEGEEKYLREIIKPMEVAAAELPKIV
IKDSAVDAICHGANLSVRGVAYVEKNVKKDSTVAIFTLKNELVAIGRALMDAEDIYRLKKGIAADIQRVMMERGVYPKVW
KSSSD

Sequences:

>Translated_325_residues
MKLENFYVKDDASTDESYGCYPTKRPMEEYIRKGLVCIDKPMGPSSHEVVVWVRRILNVSKTGHAGTLDPRVTGVLPIFI
ENATKMVKFLQESSKEYVCLMRLHGDAKREDVEKVMKLFVGRIYQRPPLKSAVKKVLRIREIYEMELLEMEGRDVLFRVV
TESGTYIRKLCRDIGEVLGTGAHMQELRRTRTGKFGEDMCYTLQDLLDAYVFWKEEGEEKYLREIIKPMEVAAAELPKIV
IKDSAVDAICHGANLSVRGVAYVEKNVKKDSTVAIFTLKNELVAIGRALMDAEDIYRLKKGIAADIQRVMMERGVYPKVW
KSSSD
>Mature_325_residues
MKLENFYVKDDASTDESYGCYPTKRPMEEYIRKGLVCIDKPMGPSSHEVVVWVRRILNVSKTGHAGTLDPRVTGVLPIFI
ENATKMVKFLQESSKEYVCLMRLHGDAKREDVEKVMKLFVGRIYQRPPLKSAVKKVLRIREIYEMELLEMEGRDVLFRVV
TESGTYIRKLCRDIGEVLGTGAHMQELRRTRTGKFGEDMCYTLQDLLDAYVFWKEEGEEKYLREIIKPMEVAAAELPKIV
IKDSAVDAICHGANLSVRGVAYVEKNVKKDSTVAIFTLKNELVAIGRALMDAEDIYRLKKGIAADIQRVMMERGVYPKVW
KSSSD

Specific function: Could be responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI215599015, Length=325, Percent_Identity=43.6923076923077, Blast_Score=254, Evalue=9e-68,
Organism=Homo sapiens, GI4503337, Length=325, Percent_Identity=43.6923076923077, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI21040257, Length=214, Percent_Identity=28.5046728971963, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI2367200, Length=247, Percent_Identity=30.3643724696356, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17553978, Length=321, Percent_Identity=38.006230529595, Blast_Score=250, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6323204, Length=325, Percent_Identity=43.3846153846154, Blast_Score=274, Evalue=1e-74,
Organism=Drosophila melanogaster, GI281364189, Length=327, Percent_Identity=41.8960244648318, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI281364187, Length=327, Percent_Identity=41.8960244648318, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI281364185, Length=327, Percent_Identity=41.8960244648318, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI281364183, Length=327, Percent_Identity=41.8960244648318, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI62471759, Length=327, Percent_Identity=41.8960244648318, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17975520, Length=327, Percent_Identity=41.8960244648318, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_ARCFU (O30001)

Other databases:

- EMBL:   AE000782
- PIR:   F69279
- RefSeq:   NP_069076.1
- ProteinModelPortal:   O30001
- SMR:   O30001
- GeneID:   1483449
- GenomeReviews:   AE000782_GR
- KEGG:   afu:AF0238
- NMPDR:   fig|224325.1.peg.235
- TIGR:   AF_0238
- HOGENOM:   HBG397258
- OMA:   SGPTSHE
- ProtClustDB:   PRK04270
- BioCyc:   AFUL224325:AF_0238-MONOMER
- HAMAP:   MF_01081
- InterPro:   IPR012960
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR004802
- InterPro:   IPR002478
- InterPro:   IPR015947
- SMART:   SM00359
- TIGRFAMs:   TIGR00425

Pfam domain/function: PF08068 DKCLD; PF01472 PUA; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 37159; Mature: 37159

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50890 PUA

Important sites: ACT_SITE 69-69

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLENFYVKDDASTDESYGCYPTKRPMEEYIRKGLVCIDKPMGPSSHEVVVWVRRILNVS
CCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCC
KTGHAGTLDPRVTGVLPIFIENATKMVKFLQESSKEYVCLMRLHGDAKREDVEKVMKLFV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
GRIYQRPPLKSAVKKVLRIREIYEMELLEMEGRDVLFRVVTESGTYIRKLCRDIGEVLGT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHHHCC
GAHMQELRRTRTGKFGEDMCYTLQDLLDAYVFWKEEGEEKYLREIIKPMEVAAAELPKIV
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHE
IKDSAVDAICHGANLSVRGVAYVEKNVKKDSTVAIFTLKNELVAIGRALMDAEDIYRLKK
ECCHHHHHHHCCCCCCEEEHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHCHHHHHHHHH
GIAADIQRVMMERGVYPKVWKSSSD
HHHHHHHHHHHHCCCCCHHCCCCCC
>Mature Secondary Structure
MKLENFYVKDDASTDESYGCYPTKRPMEEYIRKGLVCIDKPMGPSSHEVVVWVRRILNVS
CCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCC
KTGHAGTLDPRVTGVLPIFIENATKMVKFLQESSKEYVCLMRLHGDAKREDVEKVMKLFV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHH
GRIYQRPPLKSAVKKVLRIREIYEMELLEMEGRDVLFRVVTESGTYIRKLCRDIGEVLGT
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHCCCHHHHHHHHHHHHHHCC
GAHMQELRRTRTGKFGEDMCYTLQDLLDAYVFWKEEGEEKYLREIIKPMEVAAAELPKIV
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHE
IKDSAVDAICHGANLSVRGVAYVEKNVKKDSTVAIFTLKNELVAIGRALMDAEDIYRLKK
ECCHHHHHHHCCCCCCEEEHHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHCHHHHHHHHH
GIAADIQRVMMERGVYPKVWKSSSD
HHHHHHHHHHHHCCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475