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The map label for this gene is fliF [H]

Identifier: 114800445

GI number: 114800445

Start: 232128

End: 233714

Strand: Direct

Name: fliF [H]

Synonym: HNE_0242

Alternate gene names: 114800445

Gene position: 232128-233714 (Clockwise)

Preceding gene: 114797779

Following gene: 114797896

Centisome position: 6.27

GC content: 62.19

Gene sequence:

>1587_bases
ATGAAATTTCTTGATACTCTCAAAGCCCTGCCTGTTTCGCGGCAGCTGATGCTGGCCGCCGCGGTGGCGGGCATGATTGC
CGCAATGAGCTTCCTGGTTCAGGGGGCGACGCGTGATCCCATGACGTTGCTCTATTCCGGCCTTGAACCGGCCCATGCCG
GCGACATCATCAAGGAGCTGGAACAGTCTGGAACTCCCTATGAAATCAAGGGCGAAGCGATCTTTGTGCCGCAGGGAAAG
CGGGACGCGATCCGCTTCACACTGGCCCAGCAGGGGCTGCCGCGCCCGTCGGTGCAGGGCTATGAACTGCTGGACGAGGT
GAACGGGTTTTCGGTTACGTCGGAAATGTATAACGCGGCCTATTGGCGGGCCAAGGAAGGCGAGCTGACGCGGACGATCC
TGGCTATTCCCGGCGTGTCTTCGGCGCGGGTGCATATCGGCGCGAATCTGCGGTCTGGTTTCTCGCGGTCGCAGCCCAAT
CAGACCGCTTCGGTAACCCTGACGACCGCGCGTGACATGACGGCGGGCCAGGCAGAAGCCATCCAGTATATGGTGGCGCT
GGCCGTTGCGGGCCTGCGCGCCGAGGATGTGGCCGTGATCGACCCGGTGAAGGGCATTCTGGCGGGGCCGAAAGCCAACC
GGACCGAAGAACCCGCAGTGGCCGCCGCGACGCAGGCGAGCCTGCTGGAACAGAAAATCCTCAGCCTGCTGGAGCCGCGG
GTTGGCGTGGGCAATGCGCGGGTTTCGGCGACCGTGGATGTCAGCCGGCAGCGGCAGGTCACCTCGGCAGTGACATTTGA
CCCCAATTCAAGGGTCATCCGTAACCGGACGACCAATGATTCCACCGGTGCAAGCGCCGGGGCAGGCGGGGGCATGACCG
TCGCCAGCAACCTGCCACAAGGCGGCGAAGGCGGGGGCGGAAACACCTCCACCAACAAGAACTCGACCGAAACGGTCTCC
TATGAAATCAATGAAACGCGCACCGAAGTCGAAACGCTTCCCGGTGAGGTTCAGCGGATCACCATTGCGGTGTTGCTGAA
TGAGCAGGCGCTGGGAATTGATCCGGCGGCGGCCGATGTGGCCACGCTGACCCAGCAGATGGCGGCGGATTTCGAACAGC
TGATTGCTTCGGCGGCAGGGATTGATACCGCTCGGGGCGATACGATTTCGATTGAACTGATGCCGTTCCAGGCGGTGCCT
GTCGATGATCTCATCGAAGTGCCGGGCATGGTTGAGCAGCTGATGGAGCGCTATTTCTGGTCCGGATTGCAGGCTTTGCT
GTTGGGGCTGGTGGTGATCGTGCTGGGGGTCGGCGTTGTACGCCCGATGCTTGTGCAGAAATCGAAGGATGCTCTGTCTC
CGGAGGATCTTGGGGCAGGGGCGGGCGCAGAGGGTGCCGGGCGGGCCGTTGCCAGCGGCGCCGATGTGGAAAGCGACCCG
TTCGCCTATCTCAAGGATTATGCCACCGACCGGCAGGAAGAGACAGCCGCGCTGCTCCAGCAATGGCTTACGGAAGATCA
GCAGTCGGGGCAGACCGATCAGATCGCCACGGCTTTTGACAATCGCAAGGTCGCCGTGAATGAGTGA

Upstream 100 bases:

>100_bases
GTTCCTAATGACTGATCCGGGGTAAAGATAACCTTAACGATTTCGCGCCAAATATTAATCATTACAGCTGACTCGTATGC
AGCCTGGGGCGACCGCATTT

Downstream 100 bases:

>100_bases
CGCCGTATTCTCCCACCTGCCACGGTTTGATGCGGTGACCGGGCCGCGGCCCGCTGATTGGCTTCGCGCGATGACGCGGG
ATGATCTGCAGGCGCCTTCG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MKFLDTLKALPVSRQLMLAAAVAGMIAAMSFLVQGATRDPMTLLYSGLEPAHAGDIIKELEQSGTPYEIKGEAIFVPQGK
RDAIRFTLAQQGLPRPSVQGYELLDEVNGFSVTSEMYNAAYWRAKEGELTRTILAIPGVSSARVHIGANLRSGFSRSQPN
QTASVTLTTARDMTAGQAEAIQYMVALAVAGLRAEDVAVIDPVKGILAGPKANRTEEPAVAAATQASLLEQKILSLLEPR
VGVGNARVSATVDVSRQRQVTSAVTFDPNSRVIRNRTTNDSTGASAGAGGGMTVASNLPQGGEGGGGNTSTNKNSTETVS
YEINETRTEVETLPGEVQRITIAVLLNEQALGIDPAAADVATLTQQMAADFEQLIASAAGIDTARGDTISIELMPFQAVP
VDDLIEVPGMVEQLMERYFWSGLQALLLGLVVIVLGVGVVRPMLVQKSKDALSPEDLGAGAGAEGAGRAVASGADVESDP
FAYLKDYATDRQEETAALLQQWLTEDQQSGQTDQIATAFDNRKVAVNE

Sequences:

>Translated_528_residues
MKFLDTLKALPVSRQLMLAAAVAGMIAAMSFLVQGATRDPMTLLYSGLEPAHAGDIIKELEQSGTPYEIKGEAIFVPQGK
RDAIRFTLAQQGLPRPSVQGYELLDEVNGFSVTSEMYNAAYWRAKEGELTRTILAIPGVSSARVHIGANLRSGFSRSQPN
QTASVTLTTARDMTAGQAEAIQYMVALAVAGLRAEDVAVIDPVKGILAGPKANRTEEPAVAAATQASLLEQKILSLLEPR
VGVGNARVSATVDVSRQRQVTSAVTFDPNSRVIRNRTTNDSTGASAGAGGGMTVASNLPQGGEGGGGNTSTNKNSTETVS
YEINETRTEVETLPGEVQRITIAVLLNEQALGIDPAAADVATLTQQMAADFEQLIASAAGIDTARGDTISIELMPFQAVP
VDDLIEVPGMVEQLMERYFWSGLQALLLGLVVIVLGVGVVRPMLVQKSKDALSPEDLGAGAGAEGAGRAVASGADVESDP
FAYLKDYATDRQEETAALLQQWLTEDQQSGQTDQIATAFDNRKVAVNE
>Mature_528_residues
MKFLDTLKALPVSRQLMLAAAVAGMIAAMSFLVQGATRDPMTLLYSGLEPAHAGDIIKELEQSGTPYEIKGEAIFVPQGK
RDAIRFTLAQQGLPRPSVQGYELLDEVNGFSVTSEMYNAAYWRAKEGELTRTILAIPGVSSARVHIGANLRSGFSRSQPN
QTASVTLTTARDMTAGQAEAIQYMVALAVAGLRAEDVAVIDPVKGILAGPKANRTEEPAVAAATQASLLEQKILSLLEPR
VGVGNARVSATVDVSRQRQVTSAVTFDPNSRVIRNRTTNDSTGASAGAGGGMTVASNLPQGGEGGGGNTSTNKNSTETVS
YEINETRTEVETLPGEVQRITIAVLLNEQALGIDPAAADVATLTQQMAADFEQLIASAAGIDTARGDTISIELMPFQAVP
VDDLIEVPGMVEQLMERYFWSGLQALLLGLVVIVLGVGVVRPMLVQKSKDALSPEDLGAGAGAEGAGRAVASGADVESDP
FAYLKDYATDRQEETAALLQQWLTEDQQSGQTDQIATAFDNRKVAVNE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=434, Percent_Identity=32.0276497695853, Blast_Score=146, Evalue=4e-36,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 55734; Mature: 55734

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLDTLKALPVSRQLMLAAAVAGMIAAMSFLVQGATRDPMTLLYSGLEPAHAGDIIKEL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHH
EQSGTPYEIKGEAIFVPQGKRDAIRFTLAQQGLPRPSVQGYELLDEVNGFSVTSEMYNAA
HHCCCCEEECCCEEEECCCCCCHHEEEHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
YWRAKEGELTRTILAIPGVSSARVHIGANLRSGFSRSQPNQTASVTLTTARDMTAGQAEA
HEECCCCCCEEEEEECCCCCCCEEEECCCHHCCCCCCCCCCCEEEEEEECCCCCCCHHHH
IQYMVALAVAGLRAEDVAVIDPVKGILAGPKANRTEEPAVAAATQASLLEQKILSLLEPR
HHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VGVGNARVSATVDVSRQRQVTSAVTFDPNSRVIRNRTTNDSTGASAGAGGGMTVASNLPQ
CCCCCCEEEEEEECHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEECCCCC
GGEGGGGNTSTNKNSTETVSYEINETRTEVETLPGEVQRITIAVLLNEQALGIDPAAADV
CCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHH
ATLTQQMAADFEQLIASAAGIDTARGDTISIELMPFQAVPVDDLIEVPGMVEQLMERYFW
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHH
SGLQALLLGLVVIVLGVGVVRPMLVQKSKDALSPEDLGAGAGAEGAGRAVASGADVESDP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCCCC
FAYLKDYATDRQEETAALLQQWLTEDQQSGQTDQIATAFDNRKVAVNE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECC
>Mature Secondary Structure
MKFLDTLKALPVSRQLMLAAAVAGMIAAMSFLVQGATRDPMTLLYSGLEPAHAGDIIKEL
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHH
EQSGTPYEIKGEAIFVPQGKRDAIRFTLAQQGLPRPSVQGYELLDEVNGFSVTSEMYNAA
HHCCCCEEECCCEEEECCCCCCHHEEEHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHHHH
YWRAKEGELTRTILAIPGVSSARVHIGANLRSGFSRSQPNQTASVTLTTARDMTAGQAEA
HEECCCCCCEEEEEECCCCCCCEEEECCCHHCCCCCCCCCCCEEEEEEECCCCCCCHHHH
IQYMVALAVAGLRAEDVAVIDPVKGILAGPKANRTEEPAVAAATQASLLEQKILSLLEPR
HHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VGVGNARVSATVDVSRQRQVTSAVTFDPNSRVIRNRTTNDSTGASAGAGGGMTVASNLPQ
CCCCCCEEEEEEECHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCCCCEEEECCCCC
GGEGGGGNTSTNKNSTETVSYEINETRTEVETLPGEVQRITIAVLLNEQALGIDPAAADV
CCCCCCCCCCCCCCCCEEEEEEECHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCHHHHH
ATLTQQMAADFEQLIASAAGIDTARGDTISIELMPFQAVPVDDLIEVPGMVEQLMERYFW
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCHHHHHHCCHHHHHHHHHHHH
SGLQALLLGLVVIVLGVGVVRPMLVQKSKDALSPEDLGAGAGAEGAGRAVASGADVESDP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCHHCCCCCCCCCC
FAYLKDYATDRQEETAALLQQWLTEDQQSGQTDQIATAFDNRKVAVNE
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9858749; 11481430 [H]