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The map label for this gene is cpaF [C]

Identifier: 114800387

GI number: 114800387

Start: 3180047

End: 3181534

Strand: Reverse

Name: cpaF [C]

Synonym: HNE_3011

Alternate gene names: 114800387

Gene position: 3181534-3180047 (Counterclockwise)

Preceding gene: 114797644

Following gene: 114798049

Centisome position: 85.87

GC content: 60.55

Gene sequence:

>1488_bases
ATGGCTTTCGGCAAACGCTCGGGTACATCTTCCGCAACGCTGCCACCACCGGCGCCTGCGGCGGCGCCGAAGCCAGCGGC
TGCGCCATCGCCAGCTTCGGGGGCTCCGGCGGCGGCCAAGCCGCGCGCGTCTGAGCCTGCCGCGCCGCATGTCAGCAAAT
TGAAGACCCCGGAAGTGAAGCCGGAGCCGGTCCGCAAGGTCAACCAGCATTCCGATCAGTATTACGCAACGAAGACGACG
ATCTTCAATGCGCTGATCGATACGATTGATCTCTCGCAGCTCGCCCAGCTCGACACCGAAAGTGCCCGCGAAGAAATTCG
CGACATTGTGGTTGAAATTATCAGCATCAAGAATGTCGTGATGTCGATCTCCGAGCAGGAGTATCTGCTGGACGACATCT
GTAACGATGTTCTCGGCTATGGCCCGCTGGAGCCACTGCTCGCACGCGACGACATTGCCGACATCATGGTCAATGGCGCC
GACACCACCTATATCGAGGTCAACGGCAAGATCGAGCGGACCAACATCCGCTTCCGCGACAATGCGCAGCTCATGAACAT
CTGTCAGCGGATCGTGAGCCAGGTAGGCCGCCGCGTCGATGAAAGCTCCCCCATCTGTGACGCGCGCCTTCTGGATGGCT
CGCGTGTGAACGTCATTGCCCCACCGCTGGCGATTGATGGGCCGACGCTCACCATTCGTAAGTTCAAGAAAGACAAGCTG
AAACTGAACGACCTTGTACGCTTCGGCTCGATCAGCGAATGCGGCGCCGAAATCCTGCGCATCATCGGCCACAGCCGCTG
CAACGTCCTGATCTCCGGCGGTACGGGCTCGGGCAAGACGACGCTACTCAACTGTCTCACCGGCTTCATCGAGCCCGGCG
AGCGCGTCATCACCTGCGAAGACTCCGCCGAACTTCAGCTTCAGCAGCCCCATGTGGTGCGCCTCGAAACCCGCCCGCCG
AATATCGAGGGCTCGGGCGAGATCACGATGCGCGAGCTGGTCAAGAACTGTCTGCGTATGCGTCCGGAGCGGATTATCGT
GGGCGAGGTCCGCGGTCCTGAGGCGTTTGACCTTCTCCAGGCCATGAACACGGGCCATGACGGCTCGATGGGCACGCTCC
ACGCCAACAGCCCGCGCGAAGCGCTGAGCCGGGTTGAATCCATGATTACGATGGGCGGCTTCTCCCTTCCGGCAAAAACC
ATCCGTGAAATGATTGTCGGATCGATCGACATCGTCGTCCAGGCCTCGCGTCTGCGGGATGGCTCCCGCCGCATCATGTG
CATCACCGAAGTGCTCGGTCTTGAGGGCGATACGATCGTCCTTCAGGACATCATGAAATACGAGATCGATGGGGAAGATG
AAGACGGCAAGATCATCGGGCGCCATCGCGGGCTCGGTATCGGCCGGCCACGCTTCTGGGAGCGCGCGTCCTATTACAAT
CTTGAGCGCGAACTTGCTGCGGCGCTTGATGAACTGAACGGTGCCTGA

Upstream 100 bases:

>100_bases
CTGGCAGCGTCCTTAAGTGGGCGAACTCTGCAGAGGGTTGAGAAATCATTCCTCAAGAAACTGCTCGGTAAGTAGGACGA
TAAAAACGACGGGATAGAAC

Downstream 100 bases:

>100_bases
TGTCTGGTGCGATGATCCCCTATATCGTGGCTGCGCTGGCGTTTCTCGCGATTGCCGGGCTTGGTCTCGTCCTGACCGGC
GGCAATGATGATGCGGCACG

Product: pilus assembly protein CpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MAFGKRSGTSSATLPPPAPAAAPKPAAAPSPASGAPAAAKPRASEPAAPHVSKLKTPEVKPEPVRKVNQHSDQYYATKTT
IFNALIDTIDLSQLAQLDTESAREEIRDIVVEIISIKNVVMSISEQEYLLDDICNDVLGYGPLEPLLARDDIADIMVNGA
DTTYIEVNGKIERTNIRFRDNAQLMNICQRIVSQVGRRVDESSPICDARLLDGSRVNVIAPPLAIDGPTLTIRKFKKDKL
KLNDLVRFGSISECGAEILRIIGHSRCNVLISGGTGSGKTTLLNCLTGFIEPGERVITCEDSAELQLQQPHVVRLETRPP
NIEGSGEITMRELVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREALSRVESMITMGGFSLPAKT
IREMIVGSIDIVVQASRLRDGSRRIMCITEVLGLEGDTIVLQDIMKYEIDGEDEDGKIIGRHRGLGIGRPRFWERASYYN
LERELAAALDELNGA

Sequences:

>Translated_495_residues
MAFGKRSGTSSATLPPPAPAAAPKPAAAPSPASGAPAAAKPRASEPAAPHVSKLKTPEVKPEPVRKVNQHSDQYYATKTT
IFNALIDTIDLSQLAQLDTESAREEIRDIVVEIISIKNVVMSISEQEYLLDDICNDVLGYGPLEPLLARDDIADIMVNGA
DTTYIEVNGKIERTNIRFRDNAQLMNICQRIVSQVGRRVDESSPICDARLLDGSRVNVIAPPLAIDGPTLTIRKFKKDKL
KLNDLVRFGSISECGAEILRIIGHSRCNVLISGGTGSGKTTLLNCLTGFIEPGERVITCEDSAELQLQQPHVVRLETRPP
NIEGSGEITMRELVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREALSRVESMITMGGFSLPAKT
IREMIVGSIDIVVQASRLRDGSRRIMCITEVLGLEGDTIVLQDIMKYEIDGEDEDGKIIGRHRGLGIGRPRFWERASYYN
LERELAAALDELNGA
>Mature_494_residues
AFGKRSGTSSATLPPPAPAAAPKPAAAPSPASGAPAAAKPRASEPAAPHVSKLKTPEVKPEPVRKVNQHSDQYYATKTTI
FNALIDTIDLSQLAQLDTESAREEIRDIVVEIISIKNVVMSISEQEYLLDDICNDVLGYGPLEPLLARDDIADIMVNGAD
TTYIEVNGKIERTNIRFRDNAQLMNICQRIVSQVGRRVDESSPICDARLLDGSRVNVIAPPLAIDGPTLTIRKFKKDKLK
LNDLVRFGSISECGAEILRIIGHSRCNVLISGGTGSGKTTLLNCLTGFIEPGERVITCEDSAELQLQQPHVVRLETRPPN
IEGSGEITMRELVKNCLRMRPERIIVGEVRGPEAFDLLQAMNTGHDGSMGTLHANSPREALSRVESMITMGGFSLPAKTI
REMIVGSIDIVVQASRLRDGSRRIMCITEVLGLEGDTIVLQDIMKYEIDGEDEDGKIIGRHRGLGIGRPRFWERASYYNL
ERELAAALDELNGA

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=167, Percent_Identity=29.940119760479, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54005; Mature: 53873

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00227 TUBULIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFGKRSGTSSATLPPPAPAAAPKPAAAPSPASGAPAAAKPRASEPAAPHVSKLKTPEVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
PEPVRKVNQHSDQYYATKTTIFNALIDTIDLSQLAQLDTESAREEIRDIVVEIISIKNVV
CHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
MSISEQEYLLDDICNDVLGYGPLEPLLARDDIADIMVNGADTTYIEVNGKIERTNIRFRD
HHHCHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHEECCCCEEEEEECCEEEEEEEEECC
NAQLMNICQRIVSQVGRRVDESSPICDARLLDGSRVNVIAPPLAIDGPTLTIRKFKKDKL
CHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEECCEEECCCEEEHHHHHHHHH
KLNDLVRFGSISECGAEILRIIGHSRCNVLISGGTGSGKTTLLNCLTGFIEPGERVITCE
HHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEC
DSAELQLQQPHVVRLETRPPNIEGSGEITMRELVKNCLRMRPERIIVGEVRGPEAFDLLQ
CCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AMNTGHDGSMGTLHANSPREALSRVESMITMGGFSLPAKTIREMIVGSIDIVVQASRLRD
HHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHEEEHHHCCC
GSRRIMCITEVLGLEGDTIVLQDIMKYEIDGEDEDGKIIGRHRGLGIGRPRFWERASYYN
CCCEEEEEHHHHCCCCCEEHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHH
LERELAAALDELNGA
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
AFGKRSGTSSATLPPPAPAAAPKPAAAPSPASGAPAAAKPRASEPAAPHVSKLKTPEVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
PEPVRKVNQHSDQYYATKTTIFNALIDTIDLSQLAQLDTESAREEIRDIVVEIISIKNVV
CHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
MSISEQEYLLDDICNDVLGYGPLEPLLARDDIADIMVNGADTTYIEVNGKIERTNIRFRD
HHHCHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHEECCCCEEEEEECCEEEEEEEEECC
NAQLMNICQRIVSQVGRRVDESSPICDARLLDGSRVNVIAPPLAIDGPTLTIRKFKKDKL
CHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEECCEEECCCEEEHHHHHHHHH
KLNDLVRFGSISECGAEILRIIGHSRCNVLISGGTGSGKTTLLNCLTGFIEPGERVITCE
HHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEEEC
DSAELQLQQPHVVRLETRPPNIEGSGEITMRELVKNCLRMRPERIIVGEVRGPEAFDLLQ
CCCCEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
AMNTGHDGSMGTLHANSPREALSRVESMITMGGFSLPAKTIREMIVGSIDIVVQASRLRD
HHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHEEEHHHCCC
GSRRIMCITEVLGLEGDTIVLQDIMKYEIDGEDEDGKIIGRHRGLGIGRPRFWERASYYN
CCCEEEEEHHHHCCCCCEEHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHH
LERELAAALDELNGA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]