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The map label for this gene is pheS

Identifier: 114800189

GI number: 114800189

Start: 429669

End: 430745

Strand: Direct

Name: pheS

Synonym: HNE_0433

Alternate gene names: 114800189

Gene position: 429669-430745 (Clockwise)

Preceding gene: 114798827

Following gene: 114797877

Centisome position: 11.6

GC content: 63.51

Gene sequence:

>1077_bases
GTGTCCGACATAACCGCGATCGAACAAGAAGCGCTTGCCGCTATCGCTGCTGCCGCCGATCTTGCCGCGCTCGACGCCGT
GCGCGTTGCCGAGCTTGGCAAGAAGGGGCGCGTGTCCGCCCTGATGGGCGCGCTCGGCAAGATGAGCCCGGACGAGCGCA
AGGCGCAGGGGCCTCAGCTCAACGCCCTGAAGACCCGTGTGGACGATGCGATCCGCCTGCGTAAAGACGTGCTGGAGAAT
GCGGCGCTCGAGGCGCGCCTCGTGACCGAGCGCGTGGACCTGACGCTGCCGCCCGCTGCCGGCCCCGGCGAAGGCGCGCT
CCACCCGGTCATGCAGGTGTTCGAGGAAATCGCCGCCATCTTCGGCGACATGGGGTTCACCGTGGCCGAAGGGCCGGACA
TCGAAGACGACTGGCACAATTTCACGGCGCTGAACTTCCCCATCGGTCACCCGGCGCGGGAAACCCACGACACCTTCTTC
ATGAAAGCGCTGGAAGGCGACACGCCCAAAGTGCTCCGCACCCATACCAGCCCGGTGCAGGTGCGCACGATGATGGAAGA
AAAGCCGCCGATCCGCATTCTCGTGCCCGGCCGCGTCTACCGGAATGATTGGGACGCGACCCATACGCCGATGTTCCATC
AGGTCGAAGGGCTCGTCATCGAGAAGGGCATCCATATGGGCCACCTGAAAGGCTGCCTCATTGATTTCGTGAAAGCGTTC
TTCGAGGTGGACAATGTGGAATCCCGCTTCCGGCCGCATTTCTTTCCGTTCACGGAACCTTCGGCCGAGATGGATGTGAA
ATATTCCCGCAATGGCGACGCCATCGAGATCGGCGCGGGCGACCGCTGGATGGAAATCCTCGGCTGCGGCATGGTGCATC
CCAATGTGCTGCGCAATTGCGGCATCGATCCGGCCGTGCATCAGGGCTTTGCCTTCGGCATGGGGGTCGATCGCCTCGCC
ATGCTGAAATACGGCATGCCGGACCTGCGCCCCTATTTCGAGGCAGACCCGCAATGGTCGCGCCATTACGGCTTCTCACC
CTGGCTCACGCCGTCCGTTAGCGGAGGGTTGTCGTGA

Upstream 100 bases:

>100_bases
ACGCCCGTGGGTTCCGGCCTGCGGGCGTTTTGCTGATTGCCTCGCCCTTGCCCGAGCGACTAGAAAGCCGCGAAGGTCTG
ACCTGAAAGGGAAATTCCAA

Downstream 100 bases:

>100_bases
CCCTGTCTCAGGAATGGGCAGATCTGAAGCAGTTTCGCTTCGGTGATAGTCCTGAATTGGCAGACCGTCTGGCCTCTCTT
GTTGTGGCCGGCGTCAAGAA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSDITAIEQEALAAIAAAADLAALDAVRVAELGKKGRVSALMGALGKMSPDERKAQGPQLNALKTRVDDAIRLRKDVLEN
AALEARLVTERVDLTLPPAAGPGEGALHPVMQVFEEIAAIFGDMGFTVAEGPDIEDDWHNFTALNFPIGHPARETHDTFF
MKALEGDTPKVLRTHTSPVQVRTMMEEKPPIRILVPGRVYRNDWDATHTPMFHQVEGLVIEKGIHMGHLKGCLIDFVKAF
FEVDNVESRFRPHFFPFTEPSAEMDVKYSRNGDAIEIGAGDRWMEILGCGMVHPNVLRNCGIDPAVHQGFAFGMGVDRLA
MLKYGMPDLRPYFEADPQWSRHYGFSPWLTPSVSGGLS

Sequences:

>Translated_358_residues
MSDITAIEQEALAAIAAAADLAALDAVRVAELGKKGRVSALMGALGKMSPDERKAQGPQLNALKTRVDDAIRLRKDVLEN
AALEARLVTERVDLTLPPAAGPGEGALHPVMQVFEEIAAIFGDMGFTVAEGPDIEDDWHNFTALNFPIGHPARETHDTFF
MKALEGDTPKVLRTHTSPVQVRTMMEEKPPIRILVPGRVYRNDWDATHTPMFHQVEGLVIEKGIHMGHLKGCLIDFVKAF
FEVDNVESRFRPHFFPFTEPSAEMDVKYSRNGDAIEIGAGDRWMEILGCGMVHPNVLRNCGIDPAVHQGFAFGMGVDRLA
MLKYGMPDLRPYFEADPQWSRHYGFSPWLTPSVSGGLS
>Mature_357_residues
SDITAIEQEALAAIAAAADLAALDAVRVAELGKKGRVSALMGALGKMSPDERKAQGPQLNALKTRVDDAIRLRKDVLENA
ALEARLVTERVDLTLPPAAGPGEGALHPVMQVFEEIAAIFGDMGFTVAEGPDIEDDWHNFTALNFPIGHPARETHDTFFM
KALEGDTPKVLRTHTSPVQVRTMMEEKPPIRILVPGRVYRNDWDATHTPMFHQVEGLVIEKGIHMGHLKGCLIDFVKAFF
EVDNVESRFRPHFFPFTEPSAEMDVKYSRNGDAIEIGAGDRWMEILGCGMVHPNVLRNCGIDPAVHQGFAFGMGVDRLAM
LKYGMPDLRPYFEADPQWSRHYGFSPWLTPSVSGGLS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=272, Percent_Identity=31.6176470588235, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI5729820, Length=270, Percent_Identity=27.037037037037, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1788007, Length=343, Percent_Identity=53.0612244897959, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17508957, Length=272, Percent_Identity=32.7205882352941, Blast_Score=131, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=272, Percent_Identity=32.7205882352941, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=30.4182509505703, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=377, Percent_Identity=28.9124668435013, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325304, Length=284, Percent_Identity=25, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI18858079, Length=273, Percent_Identity=30.03663003663, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI17137424, Length=247, Percent_Identity=25.9109311740891, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_HYPNA (Q0C530)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759163.1
- ProteinModelPortal:   Q0C530
- SMR:   Q0C530
- STRING:   Q0C530
- GeneID:   4290033
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0433
- NMPDR:   fig|228405.5.peg.426
- TIGR:   HNE_0433
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q0C530
- ProtClustDB:   PRK00488
- BioCyc:   HNEP81032:HNE_0433-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39484; Mature: 39352

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDITAIEQEALAAIAAAADLAALDAVRVAELGKKGRVSALMGALGKMSPDERKAQGPQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCCH
NALKTRVDDAIRLRKDVLENAALEARLVTERVDLTLPPAAGPGEGALHPVMQVFEEIAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHH
FGDMGFTVAEGPDIEDDWHNFTALNFPIGHPARETHDTFFMKALEGDTPKVLRTHTSPVQ
HCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHEECCCCCHH
VRTMMEEKPPIRILVPGRVYRNDWDATHTPMFHQVEGLVIEKGIHMGHLKGCLIDFVKAF
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCEECCCCHHHHHHHHHHHHHHH
FEVDNVESRFRPHFFPFTEPSAEMDVKYSRNGDAIEIGAGDRWMEILGCGMVHPNVLRNC
HHHCCHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCCCCCHHHHHHC
GIDPAVHQGFAFGMGVDRLAMLKYGMPDLRPYFEADPQWSRHYGFSPWLTPSVSGGLS
CCCHHHHCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDITAIEQEALAAIAAAADLAALDAVRVAELGKKGRVSALMGALGKMSPDERKAQGPQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCCH
NALKTRVDDAIRLRKDVLENAALEARLVTERVDLTLPPAAGPGEGALHPVMQVFEEIAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHH
FGDMGFTVAEGPDIEDDWHNFTALNFPIGHPARETHDTFFMKALEGDTPKVLRTHTSPVQ
HCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHEECCCCCHH
VRTMMEEKPPIRILVPGRVYRNDWDATHTPMFHQVEGLVIEKGIHMGHLKGCLIDFVKAF
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCCCEECCCCHHHHHHHHHHHHHHH
FEVDNVESRFRPHFFPFTEPSAEMDVKYSRNGDAIEIGAGDRWMEILGCGMVHPNVLRNC
HHHCCHHHHCCCCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHCCCCCHHHHHHC
GIDPAVHQGFAFGMGVDRLAMLKYGMPDLRPYFEADPQWSRHYGFSPWLTPSVSGGLS
CCCHHHHCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA