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The map label for this gene is mdh

Identifier: 114799989

GI number: 114799989

Start: 3387921

End: 3388883

Strand: Reverse

Name: mdh

Synonym: HNE_3218

Alternate gene names: 114799989

Gene position: 3388883-3387921 (Counterclockwise)

Preceding gene: 114797078

Following gene: 114798959

Centisome position: 91.47

GC content: 62.2

Gene sequence:

>963_bases
ATGGCCCGCAATAAAATCGCGCTGATCGGCTCCGGCATGATCGGCGGTACTCTCGCCCACGTTGCTGCCCGCGAAGAACT
TGGGGATGTCATCCTGTTCGACATCGCCGAGGGCCTGCCTCAGGGCAAAGGTCTCGACATTGCCGAGTCCACGCCTGTCT
ATGGCGCGTCCGTCAATCTCAAGGGCGTGAACGACTATGCCGGCATCGCGGGCGCCGATGTCTGCATCGTCACGGCCGGC
GTGCCGCGCAAGCCGGGCATGAGCCGCGACGATCTTCTCGGCATCAACCTGAAAGTCATGAAAGCCGTCGGCGAAGGCAT
CAAGGCCCACGCTCCGAACGCTTTCGTTATCTGCATCACCAACCCGCTCGACGCGATGGTCTGGGCGCTGCAGAAATTCT
CCGGCCTGCCGACTGAAAAAGTCGTGGGTATGGCAGGCGTGCTCGACAGCTCGCGTTTCGCCTACTTCCTCTCCGAGAAA
ACCGGTGTCTCCGTAGCAGACATCCATGCCTGGACGCTGGGCGGCCACGGCGACGACATGGTGCCGATGGTGCGCCACTC
CACCGTGGGCGGCCTGCCGCTGACGCAGCTCGTCGCGCAGGGCTGGATGAGCCAGGCGGAGCTGGATGCGATTGTCGAGC
GTACCCGCAAAGGCGGCGGTGAGATCGTCAACCTCCTGAAAACCGGCTCGGCCTATTACGCGCCTGCCGAGTCCGCCATT
GCGATGGCCAAGTCCTACCTCGCCGACCAGAAGCGCGTGCTTCCGGTGGCTTCGTATTGCTCGGGCCAGTACGGTCTGAA
GGACATGTATGTCGGCGTGCCAACGCTGATCGGCGCCGGCGGCGCCGAGAAGATCGTCGAGTTCGACTTCAACGCAGACG
AAAAAGCCATGTTCGACAAGTCCGTTGCTTCGGTGAACGGTCTGATCGAAGCCTGTAAGGGCATTGATCCTTCGCTGGCC
TAA

Upstream 100 bases:

>100_bases
TCGCGGGGCGTTTCACATTCTCGCCGCATTGCAACACACGCCTAGCTCGGCTAGGGCTCCGCCAGCTTGTTTCCACGACC
ATTTTTCTCAGGAGAAACAC

Downstream 100 bases:

>100_bases
GCCAGGCGATGATCATCGTTGAAGGCTATGTCAAATTTGCGGATGCGGGCGATTTTGCCCGCGTCCGCGACGCTGCCTTC
GCCCAGATGAGCGCAAGCCG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MARNKIALIGSGMIGGTLAHVAAREELGDVILFDIAEGLPQGKGLDIAESTPVYGASVNLKGVNDYAGIAGADVCIVTAG
VPRKPGMSRDDLLGINLKVMKAVGEGIKAHAPNAFVICITNPLDAMVWALQKFSGLPTEKVVGMAGVLDSSRFAYFLSEK
TGVSVADIHAWTLGGHGDDMVPMVRHSTVGGLPLTQLVAQGWMSQAELDAIVERTRKGGGEIVNLLKTGSAYYAPAESAI
AMAKSYLADQKRVLPVASYCSGQYGLKDMYVGVPTLIGAGGAEKIVEFDFNADEKAMFDKSVASVNGLIEACKGIDPSLA

Sequences:

>Translated_320_residues
MARNKIALIGSGMIGGTLAHVAAREELGDVILFDIAEGLPQGKGLDIAESTPVYGASVNLKGVNDYAGIAGADVCIVTAG
VPRKPGMSRDDLLGINLKVMKAVGEGIKAHAPNAFVICITNPLDAMVWALQKFSGLPTEKVVGMAGVLDSSRFAYFLSEK
TGVSVADIHAWTLGGHGDDMVPMVRHSTVGGLPLTQLVAQGWMSQAELDAIVERTRKGGGEIVNLLKTGSAYYAPAESAI
AMAKSYLADQKRVLPVASYCSGQYGLKDMYVGVPTLIGAGGAEKIVEFDFNADEKAMFDKSVASVNGLIEACKGIDPSLA
>Mature_319_residues
ARNKIALIGSGMIGGTLAHVAAREELGDVILFDIAEGLPQGKGLDIAESTPVYGASVNLKGVNDYAGIAGADVCIVTAGV
PRKPGMSRDDLLGINLKVMKAVGEGIKAHAPNAFVICITNPLDAMVWALQKFSGLPTEKVVGMAGVLDSSRFAYFLSEKT
GVSVADIHAWTLGGHGDDMVPMVRHSTVGGLPLTQLVAQGWMSQAELDAIVERTRKGGGEIVNLLKTGSAYYAPAESAIA
MAKSYLADQKRVLPVASYCSGQYGLKDMYVGVPTLIGAGGAEKIVEFDFNADEKAMFDKSVASVNGLIEACKGIDPSLA

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=30.1948051948052, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=30.1948051948052, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI47059044, Length=309, Percent_Identity=27.831715210356, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI221136809, Length=309, Percent_Identity=27.831715210356, Blast_Score=148, Evalue=8e-36,
Organism=Homo sapiens, GI5031857, Length=310, Percent_Identity=27.741935483871, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI260099723, Length=310, Percent_Identity=27.741935483871, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI291575128, Length=309, Percent_Identity=28.1553398058252, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4557032, Length=309, Percent_Identity=28.1553398058252, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=24.7557003257329, Blast_Score=131, Evalue=8e-31,
Organism=Homo sapiens, GI260099727, Length=196, Percent_Identity=30.6122448979592, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI260099725, Length=196, Percent_Identity=30.6122448979592, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI207028494, Length=193, Percent_Identity=28.4974093264249, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI21735621, Length=292, Percent_Identity=26.3698630136986, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI103472011, Length=314, Percent_Identity=21.0191082802548, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789632, Length=314, Percent_Identity=27.3885350318471, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17535107, Length=292, Percent_Identity=25.3424657534247, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554310, Length=264, Percent_Identity=29.1666666666667, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322765, Length=234, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320125, Length=166, Percent_Identity=28.9156626506024, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136226, Length=302, Percent_Identity=26.8211920529801, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=24.9201277955272, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647881, Length=258, Percent_Identity=27.906976744186, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24663595, Length=163, Percent_Identity=29.4478527607362, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24663599, Length=227, Percent_Identity=26.431718061674, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_HYPNA (Q0BXA0)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761893.1
- ProteinModelPortal:   Q0BXA0
- SMR:   Q0BXA0
- STRING:   Q0BXA0
- GeneID:   4290204
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3218
- NMPDR:   fig|228405.5.peg.3082
- TIGR:   HNE_3218
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- PhylomeDB:   Q0BXA0
- ProtClustDB:   PRK06223
- BioCyc:   HNEP81032:HNE_3218-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33388; Mature: 33257

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARNKIALIGSGMIGGTLAHVAAREELGDVILFDIAEGLPQGKGLDIAESTPVYGASVNL
CCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCCCCCCEECEEEE
KGVNDYAGIAGADVCIVTAGVPRKPGMSRDDLLGINLKVMKAVGEGIKAHAPNAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEEEC
NPLDAMVWALQKFSGLPTEKVVGMAGVLDSSRFAYFLSEKTGVSVADIHAWTLGGHGDDM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCCCCCC
VPMVRHSTVGGLPLTQLVAQGWMSQAELDAIVERTRKGGGEIVNLLKTGSAYYAPAESAI
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEECCHHHHH
AMAKSYLADQKRVLPVASYCSGQYGLKDMYVGVPTLIGAGGAEKIVEFDFNADEKAMFDK
HHHHHHHHCCHHCCHHHHHCCCCCCCHHHHCCCHHHHCCCCCCEEEEECCCCCHHHHHHH
SVASVNGLIEACKGIDPSLA
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARNKIALIGSGMIGGTLAHVAAREELGDVILFDIAEGLPQGKGLDIAESTPVYGASVNL
CCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEHHHCCCCCCCCCCCCCCCCEECEEEE
KGVNDYAGIAGADVCIVTAGVPRKPGMSRDDLLGINLKVMKAVGEGIKAHAPNAFVICIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCCCCEEEEEEC
NPLDAMVWALQKFSGLPTEKVVGMAGVLDSSRFAYFLSEKTGVSVADIHAWTLGGHGDDM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEECCCCCCC
VPMVRHSTVGGLPLTQLVAQGWMSQAELDAIVERTRKGGGEIVNLLKTGSAYYAPAESAI
CHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEECCHHHHH
AMAKSYLADQKRVLPVASYCSGQYGLKDMYVGVPTLIGAGGAEKIVEFDFNADEKAMFDK
HHHHHHHHCCHHCCHHHHHCCCCCCCHHHHCCCHHHHCCCCCCEEEEECCCCCHHHHHHH
SVASVNGLIEACKGIDPSLA
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA