Click here to switch to the map view.

The map label for this gene is thrS

Identifier: 114799933

GI number: 114799933

Start: 3370437

End: 3372410

Strand: Reverse

Name: thrS

Synonym: HNE_3196

Alternate gene names: 114799933

Gene position: 3372410-3370437 (Counterclockwise)

Preceding gene: 114798636

Following gene: 114797140

Centisome position: 91.02

GC content: 62.11

Gene sequence:

>1974_bases
ATGATAAACGTCACGCTGCCCGACGGTTCCAAACGCGAATATGCTGAAGGTGCCTCGCCTCTCGATGTTGCCGAGTCGAT
TTCCAAATCCCTCGCCAAAAAGGCGCTCGCCGCCAAGGTCGATGGCCAGATGTGGGATCTTGTCCGCCCGCTGGAAGGCG
ATGCCAGCGTCGCCATCATCACCGACCGCGACCCTGAAGGCCTGGAGCTGATCCGCCACGACGCGGCCCACGTCCTCGCC
CAGGCGGTGCAGGAACTTTATCCTGACACGCAGGTGACCATCGGCCCGGTCATTGATGACGGCTTTTACTATGACTTTGC
CCGGAAAGAGCCGTTCTCGACGGACGACTTCGACAAGATCGAAGCCCGGATGCGCGAGATCGTCGACGCCGATTATCCGA
TCGTGCGCGAGGTTCTGGGCAAGGACGAGGCAATCGCAACGTTCAAGAGCCTCGGCGAAGAGTACAAGGCCCAGATCATC
GACGACATCATCCCGCCGGGCGAACAGATCACGGTCTATCGTCAGGGCAAATGGTTTGACCTTTGCCGCGGGCCACACCT
GCCGTCCACGGGCAAGCTGCCCAAGGCGTTCAAGCTGATGAAGCTCGCCGGGGCCTATTGGCGCGGCGACCATCGCAACG
AAATGCTCCAGCGGATGTACGGCACGGCCTGGGCCAATGAGAAAGACCTCAAGGACCACCTCACGCGCCTGGAAGAGGCC
GAGAAACGCGATCACCGGAAGCTCGGCGCTCAGCTCGACCTGTTCCATTTCCAGCCAGAAGCGCAAGGCTCGGTGTTCTG
GCATCCCAAAGGCTTCATGATCTGGCGCGAACTGGAGGCCTACATCCGCCGCCAGCAGGACGCGGACGGCTATGTCGAGG
TCAAGACGCCGCAACTCCTGAACTCCGTCTTCTGGGAGAAGTCCGGCCACTGGTCGAAGTTCCGCGAGAACATGTTCGTC
GTTCCCGACGAGATCCCCTCGACCGAGAGCGACGCCCCGATCCTGTCAGGGGAGGGCGAACTGATGGCCCTGAAGCCGAT
GAACTGCCCCGCGCATGTCCAGATCTTCAAGAATGCCCAGCGCTCCTATCGCGACCTGCCGATCCGCATGGCGGAGTTTG
GATGCTGCCACCGCAACGAAGCCCATGGCGCGCTGCATGGCCTGATGCGGGTGCGTCAGATGACCCAGGATGATGCGCAC
ATCTTCTGCCGGGAAGACCAGATCGCTGACGAGACCCTGCGGTTCCTGAAACTGTTTGAAACCGTTTATGGTGATTTCGG
CCTGTCGGAGATTTCCTACAAGCTGGCGACCCGGCCGGAAATGCGGGCGGGTACGGACGAAACCTGGGACCGCGCAGAGA
AAGCGCTCGCCGACGCCCTGACGGCGGCCGGGATGACCTTCTCCATCGCGGAAGGGGAGGGCGCGTTCTACGGACCGAAA
CTCGAATTCCACCTGACCGACGCCATCGGCCGCACCTGGCAGTGCGGTACGTTTCAGCTCGACTATGTCCTGCCCGAACG
TCTTGATGCCGAATATACCGGCTCTGACGGCGCCAAGCACCGGCCGGTGATGCTTCACCGCGCGGTGTTCGGCACGTTCG
AGCGCTTCATGGGCATTCTGATCGAGAACTATGCCGGCAGCTTCCCCCTCTGGATGTCGCCCGTTCAGGTCGTCGTCGCC
GCCATCACCGATGCGGCCGGTGACTATGCCGAGGAAGCCGCCGCTGCCCTGCGCAAGGCCGGGCTCCGCGTCGAAGTCGA
CAAGCGCAACGAGACGATCAACTACAAGGTCCGTGAGCATTCGGTTCAGAAGGTTCCCGTGATTGCCGTTGTGGGCGCGC
GGGAAGCTGAAGATCGCAAACTGGCGCTGCGGCGGCTGGGTTCGAATGGTCAGCAGATCATCTCTCTGGACGAGGCGCTG
GTCGCCCTTGCAGACGAGGCGCGCGCGCCGGATCTCAAGCGGGCCCGCAGTTAA

Upstream 100 bases:

>100_bases
TCACTGCGGCGCCAGAAGCCTTGAAGTAGCCCTTGAAGCCGGTCCCTTTCTATTCCACCGCGGGAGACCCCTGCGGCCCG
CCTGAGAAACTGAAAAGATC

Downstream 100 bases:

>100_bases
GGGCCGATGACAAGCCCGTCATCAGTGGTCAGAGTGTCTGGCATGGCTTACGGACCCGTCTTCGCTGGCCTGTTGCTGCT
CTGGCCGCTTCTGGCGGTGC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 657; Mature: 657

Protein sequence:

>657_residues
MINVTLPDGSKREYAEGASPLDVAESISKSLAKKALAAKVDGQMWDLVRPLEGDASVAIITDRDPEGLELIRHDAAHVLA
QAVQELYPDTQVTIGPVIDDGFYYDFARKEPFSTDDFDKIEARMREIVDADYPIVREVLGKDEAIATFKSLGEEYKAQII
DDIIPPGEQITVYRQGKWFDLCRGPHLPSTGKLPKAFKLMKLAGAYWRGDHRNEMLQRMYGTAWANEKDLKDHLTRLEEA
EKRDHRKLGAQLDLFHFQPEAQGSVFWHPKGFMIWRELEAYIRRQQDADGYVEVKTPQLLNSVFWEKSGHWSKFRENMFV
VPDEIPSTESDAPILSGEGELMALKPMNCPAHVQIFKNAQRSYRDLPIRMAEFGCCHRNEAHGALHGLMRVRQMTQDDAH
IFCREDQIADETLRFLKLFETVYGDFGLSEISYKLATRPEMRAGTDETWDRAEKALADALTAAGMTFSIAEGEGAFYGPK
LEFHLTDAIGRTWQCGTFQLDYVLPERLDAEYTGSDGAKHRPVMLHRAVFGTFERFMGILIENYAGSFPLWMSPVQVVVA
AITDAAGDYAEEAAAALRKAGLRVEVDKRNETINYKVREHSVQKVPVIAVVGAREAEDRKLALRRLGSNGQQIISLDEAL
VALADEARAPDLKRARS

Sequences:

>Translated_657_residues
MINVTLPDGSKREYAEGASPLDVAESISKSLAKKALAAKVDGQMWDLVRPLEGDASVAIITDRDPEGLELIRHDAAHVLA
QAVQELYPDTQVTIGPVIDDGFYYDFARKEPFSTDDFDKIEARMREIVDADYPIVREVLGKDEAIATFKSLGEEYKAQII
DDIIPPGEQITVYRQGKWFDLCRGPHLPSTGKLPKAFKLMKLAGAYWRGDHRNEMLQRMYGTAWANEKDLKDHLTRLEEA
EKRDHRKLGAQLDLFHFQPEAQGSVFWHPKGFMIWRELEAYIRRQQDADGYVEVKTPQLLNSVFWEKSGHWSKFRENMFV
VPDEIPSTESDAPILSGEGELMALKPMNCPAHVQIFKNAQRSYRDLPIRMAEFGCCHRNEAHGALHGLMRVRQMTQDDAH
IFCREDQIADETLRFLKLFETVYGDFGLSEISYKLATRPEMRAGTDETWDRAEKALADALTAAGMTFSIAEGEGAFYGPK
LEFHLTDAIGRTWQCGTFQLDYVLPERLDAEYTGSDGAKHRPVMLHRAVFGTFERFMGILIENYAGSFPLWMSPVQVVVA
AITDAAGDYAEEAAAALRKAGLRVEVDKRNETINYKVREHSVQKVPVIAVVGAREAEDRKLALRRLGSNGQQIISLDEAL
VALADEARAPDLKRARS
>Mature_657_residues
MINVTLPDGSKREYAEGASPLDVAESISKSLAKKALAAKVDGQMWDLVRPLEGDASVAIITDRDPEGLELIRHDAAHVLA
QAVQELYPDTQVTIGPVIDDGFYYDFARKEPFSTDDFDKIEARMREIVDADYPIVREVLGKDEAIATFKSLGEEYKAQII
DDIIPPGEQITVYRQGKWFDLCRGPHLPSTGKLPKAFKLMKLAGAYWRGDHRNEMLQRMYGTAWANEKDLKDHLTRLEEA
EKRDHRKLGAQLDLFHFQPEAQGSVFWHPKGFMIWRELEAYIRRQQDADGYVEVKTPQLLNSVFWEKSGHWSKFRENMFV
VPDEIPSTESDAPILSGEGELMALKPMNCPAHVQIFKNAQRSYRDLPIRMAEFGCCHRNEAHGALHGLMRVRQMTQDDAH
IFCREDQIADETLRFLKLFETVYGDFGLSEISYKLATRPEMRAGTDETWDRAEKALADALTAAGMTFSIAEGEGAFYGPK
LEFHLTDAIGRTWQCGTFQLDYVLPERLDAEYTGSDGAKHRPVMLHRAVFGTFERFMGILIENYAGSFPLWMSPVQVVVA
AITDAAGDYAEEAAAALRKAGLRVEVDKRNETINYKVREHSVQKVPVIAVVGAREAEDRKLALRRLGSNGQQIISLDEAL
VALADEARAPDLKRARS

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=641, Percent_Identity=39.9375975039002, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI38570111, Length=628, Percent_Identity=39.171974522293, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI20070344, Length=663, Percent_Identity=38.3107088989442, Blast_Score=422, Evalue=1e-118,
Organism=Escherichia coli, GI1788013, Length=651, Percent_Identity=48.6943164362519, Blast_Score=666, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=643, Percent_Identity=40.5909797822706, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI71984192, Length=643, Percent_Identity=40.5909797822706, Blast_Score=483, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322112, Length=677, Percent_Identity=38.9955686853767, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322654, Length=399, Percent_Identity=35.3383458646617, Blast_Score=270, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24583839, Length=645, Percent_Identity=39.5348837209302, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24583842, Length=645, Percent_Identity=39.5348837209302, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574141, Length=645, Percent_Identity=39.5348837209302, Blast_Score=473, Evalue=1e-133,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_HYPNA (Q0BXC2)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761871.1
- ProteinModelPortal:   Q0BXC2
- SMR:   Q0BXC2
- STRING:   Q0BXC2
- GeneID:   4290087
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3196
- NMPDR:   fig|228405.5.peg.3061
- TIGR:   HNE_3196
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- PhylomeDB:   Q0BXC2
- ProtClustDB:   PRK00413
- BioCyc:   HNEP81032:HNE_3196-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 74099; Mature: 74099

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVTLPDGSKREYAEGASPLDVAESISKSLAKKALAAKVDGQMWDLVRPLEGDASVAII
CEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEE
TDRDPEGLELIRHDAAHVLAQAVQELYPDTQVTIGPVIDDGFYYDFARKEPFSTDDFDKI
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCEEEECCCCCCCCCCCHHHH
EARMREIVDADYPIVREVLGKDEAIATFKSLGEEYKAQIIDDIIPPGEQITVYRQGKWFD
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
LCRGPHLPSTGKLPKAFKLMKLAGAYWRGDHRNEMLQRMYGTAWANEKDLKDHLTRLEEA
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EKRDHRKLGAQLDLFHFQPEAQGSVFWHPKGFMIWRELEAYIRRQQDADGYVEVKTPQLL
HHHHHHHHCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHH
NSVFWEKSGHWSKFRENMFVVPDEIPSTESDAPILSGEGELMALKPMNCPAHVQIFKNAQ
HHHHHCCCCCHHHHHCCEEEECCCCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHH
RSYRDLPIRMAEFGCCHRNEAHGALHGLMRVRQMTQDDAHIFCREDQIADETLRFLKLFE
HHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
TVYGDFGLSEISYKLATRPEMRAGTDETWDRAEKALADALTAAGMTFSIAEGEGAFYGPK
HHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCE
LEFHLTDAIGRTWQCGTFQLDYVLPERLDAEYTGSDGAKHRPVMLHRAVFGTFERFMGIL
EEEEEEHHCCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IENYAGSFPLWMSPVQVVVAAITDAAGDYAEEAAAALRKAGLRVEVDKRNETINYKVREH
HHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCC
SVQKVPVIAVVGAREAEDRKLALRRLGSNGQQIISLDEALVALADEARAPDLKRARS
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MINVTLPDGSKREYAEGASPLDVAESISKSLAKKALAAKVDGQMWDLVRPLEGDASVAII
CEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEE
TDRDPEGLELIRHDAAHVLAQAVQELYPDTQVTIGPVIDDGFYYDFARKEPFSTDDFDKI
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEECCCEEEECCCCCCCCCCCHHHH
EARMREIVDADYPIVREVLGKDEAIATFKSLGEEYKAQIIDDIIPPGEQITVYRQGKWFD
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
LCRGPHLPSTGKLPKAFKLMKLAGAYWRGDHRNEMLQRMYGTAWANEKDLKDHLTRLEEA
HHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EKRDHRKLGAQLDLFHFQPEAQGSVFWHPKGFMIWRELEAYIRRQQDADGYVEVKTPQLL
HHHHHHHHCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHH
NSVFWEKSGHWSKFRENMFVVPDEIPSTESDAPILSGEGELMALKPMNCPAHVQIFKNAQ
HHHHHCCCCCHHHHHCCEEEECCCCCCCCCCCCEECCCCCEEEEECCCCCHHHHHHHHHH
RSYRDLPIRMAEFGCCHRNEAHGALHGLMRVRQMTQDDAHIFCREDQIADETLRFLKLFE
HHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHH
TVYGDFGLSEISYKLATRPEMRAGTDETWDRAEKALADALTAAGMTFSIAEGEGAFYGPK
HHHCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCE
LEFHLTDAIGRTWQCGTFQLDYVLPERLDAEYTGSDGAKHRPVMLHRAVFGTFERFMGIL
EEEEEEHHCCCCCCCCCEEEEEECHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
IENYAGSFPLWMSPVQVVVAAITDAAGDYAEEAAAALRKAGLRVEVDKRNETINYKVREH
HHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCC
SVQKVPVIAVVGAREAEDRKLALRRLGSNGQQIISLDEALVALADEARAPDLKRARS
CCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA