Click here to switch to the map view.

The map label for this gene is rnhA

Identifier: 114799775

GI number: 114799775

Start: 960110

End: 960562

Strand: Reverse

Name: rnhA

Synonym: HNE_0945

Alternate gene names: 114799775

Gene position: 960562-960110 (Counterclockwise)

Preceding gene: 114800157

Following gene: 114797525

Centisome position: 25.93

GC content: 60.49

Gene sequence:

>453_bases
ATGACCGACAAAGACATGATTGAAATCTGGACTGATGGCGCGTGTAGCGGAAATCCCGGCCCCGGCGGCTGGGGCGCGCT
GATCCGCTGGAATGGCCACGAAAAGGAACTCTATGGCGGCGATCCGGCCACCACCAACAACCGGATGGAAATGACCGCCG
TGATCGAGGCGCTGAACGCGCTCAACCGCCCCTCAAAGATCACGCTGAACGTGGATTCCACCTATGTGAAGGACGGCCTG
ACCAAATGGATCAAGGGCTGGAAGCGCAATGGCTGGAAAACCGCCGACAAGAAGCCTGTGAAAAACCAGGAGCTGTGGAT
GGCGATGGAAGAAGCCTGCAAACGGCACGAGATCACCTGGGTCTGGGTGAAGGGCCATGCGGGCGATGAGGGTAACGAGC
GGGCCGACGGCCTCGCCCGCAAGGGCACGGATGAAGTCCGCGGGCGGAAATAG

Upstream 100 bases:

>100_bases
CACGCCATCGTACAGCCGGCCCGCCAGCTTACACAGATGGATGTGAGTGTCTTCTGAAGGGCGCTTGACGCGGGCGCCAA
AGCCCCGCACCCCGCTGATC

Downstream 100 bases:

>100_bases
GCGCGCTTCAAATCCGCAATGAAAAACGCCCGGAGAGTCTGGCTCTCCGGGCGTAAATTTTTGACGGGTCTCAGAAAGCT
CACTCTCAGATAATTCAGGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MTDKDMIEIWTDGACSGNPGPGGWGALIRWNGHEKELYGGDPATTNNRMEMTAVIEALNALNRPSKITLNVDSTYVKDGL
TKWIKGWKRNGWKTADKKPVKNQELWMAMEEACKRHEITWVWVKGHAGDEGNERADGLARKGTDEVRGRK

Sequences:

>Translated_150_residues
MTDKDMIEIWTDGACSGNPGPGGWGALIRWNGHEKELYGGDPATTNNRMEMTAVIEALNALNRPSKITLNVDSTYVKDGL
TKWIKGWKRNGWKTADKKPVKNQELWMAMEEACKRHEITWVWVKGHAGDEGNERADGLARKGTDEVRGRK
>Mature_149_residues
TDKDMIEIWTDGACSGNPGPGGWGALIRWNGHEKELYGGDPATTNNRMEMTAVIEALNALNRPSKITLNVDSTYVKDGLT
KWIKGWKRNGWKTADKKPVKNQELWMAMEEACKRHEITWVWVKGHAGDEGNERADGLARKGTDEVRGRK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=151, Percent_Identity=37.0860927152318, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1786408, Length=134, Percent_Identity=56.7164179104478, Blast_Score=164, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI115532542, Length=139, Percent_Identity=38.1294964028777, Blast_Score=92, Evalue=9e-20,
Organism=Drosophila melanogaster, GI45552509, Length=144, Percent_Identity=34.0277777777778, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17137476, Length=144, Percent_Identity=34.0277777777778, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_HYPNA (Q0C3M1)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759672.1
- ProteinModelPortal:   Q0C3M1
- SMR:   Q0C3M1
- STRING:   Q0C3M1
- GeneID:   4289792
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0945
- NMPDR:   fig|228405.5.peg.924
- TIGR:   HNE_0945
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q0C3M1
- BioCyc:   HNEP81032:HNE_0945-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16853; Mature: 16722

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKDMIEIWTDGACSGNPGPGGWGALIRWNGHEKELYGGDPATTNNRMEMTAVIEALNA
CCCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHH
LNRPSKITLNVDSTYVKDGLTKWIKGWKRNGWKTADKKPVKNQELWMAMEEACKRHEITW
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEE
VWVKGHAGDEGNERADGLARKGTDEVRGRK
EEEECCCCCCCCCHHHHHHHCCCHHHCCCC
>Mature Secondary Structure 
TDKDMIEIWTDGACSGNPGPGGWGALIRWNGHEKELYGGDPATTNNRMEMTAVIEALNA
CCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHHHH
LNRPSKITLNVDSTYVKDGLTKWIKGWKRNGWKTADKKPVKNQELWMAMEEACKRHEITW
CCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEE
VWVKGHAGDEGNERADGLARKGTDEVRGRK
EEEECCCCCCCCCHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA