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The map label for this gene is mraW

Identifier: 114799717

GI number: 114799717

Start: 3202287

End: 3203315

Strand: Reverse

Name: mraW

Synonym: HNE_3032

Alternate gene names: 114799717

Gene position: 3203315-3202287 (Counterclockwise)

Preceding gene: 114800447

Following gene: 114797112

Centisome position: 86.46

GC content: 65.31

Gene sequence:

>1029_bases
ATGTCTTTGCGAACCACGCCCCGTAGCCCCGCCGAGCCGGGCCATCTGCCGGTCATGCTGGCCGAAGTGCTGACCGCGCT
CGCGCCGGCTGACGGCGAGGTGATCGTCGATGGCACATTCGGCGGTGGCGGCTACACAACGGCCATTCTCAAGGCCGCCA
ATTGCACCGTACTCGGCATTGACCGCGACCTCGACGCGATCGTCCGGGCAGAGAAGATGGCGCAGGCCAATCCGCGCCTT
GTGCCGTTGCTGGGCTGTTTTGGCGATCTCGATACATTGGCCGAGCAGGCCGGGCATGCGTCCGTAGACGGTGTCGTGCT
GGATATTGGCGTGTCCAGCTTCCAGATCGACCAGGCCGATCGCGGCTTCTCGTTCATGAAGGATGGCCCGCTCGACATGC
GTATGGGCGGCAGTGGTCCGTCGGCTGCCGATGTGGTCAACACGATGAGTGAGAAGGGCCTGGCGGACGTGATCTTCCGC
CTCGGCGAAGAGAAAAACTCCCGGCGGATTGCCCGCGTGCTGGTTCAGCGCCGGGTACAGGCTCCGTTTGAAACCACTGC
AGATCTTGCGGCGGTTGTCGAGGAAGCTGTGGGCGGACGGCGCGGGGCGCGGTCGCACCCGGCGACGCTGACCTTTCAGG
CGATCCGCATGTATGTGAACGACGAACTGGGAGAGCTGGCCCGCGGCCTGCGCGCAGCAGAGCGCATATTGAAGCCCGGC
GGGCGGCTGGTGGTGGTGACGTTCCACTCGCTGGAGGACCGGCTCGTCAAGCAATGGTTGCGCGAGCGGGCAGGCGCGGT
GCCGGGCGGTTCGCGCCACATGCCGCTGATGTCCAAAGGCCCGGCGCCCGCCTTTGAATTGCAGGAAAACAAGGCGGTGC
AGCCGTCTGAAAAGGAAGTGGAAACCAATCCACGCGCACGATCTGCCAAGTTGCGGGCGGCGATCCGCACCAACGCGGAC
GCGGCCCTGGATGAGGCGAGCGACGGTATGAACTTGCCGCCGCTCGCGGAGCTGGAGAAGTCGCGATGA

Upstream 100 bases:

>100_bases
CCATCGTTACCGCGCCCGAAACGCTCGATGCCTTTGCCTCCGCGTATAACCGGGTGCGTCAGCGCCGGATGGCGGCTGGC
CGGGGCGGGGAGGGCAGCTG

Downstream 100 bases:

>100_bases
GCCGCAAGGTATTTTTCTTTGGTCTTCTGGTCGCCGGGTTGCTGATTTTCAGCCTCTACCGGGCAAAATACGGCGCCAAG
GACACGGCCGCCGAGCTGAT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSLRTTPRSPAEPGHLPVMLAEVLTALAPADGEVIVDGTFGGGGYTTAILKAANCTVLGIDRDLDAIVRAEKMAQANPRL
VPLLGCFGDLDTLAEQAGHASVDGVVLDIGVSSFQIDQADRGFSFMKDGPLDMRMGGSGPSAADVVNTMSEKGLADVIFR
LGEEKNSRRIARVLVQRRVQAPFETTADLAAVVEEAVGGRRGARSHPATLTFQAIRMYVNDELGELARGLRAAERILKPG
GRLVVVTFHSLEDRLVKQWLRERAGAVPGGSRHMPLMSKGPAPAFELQENKAVQPSEKEVETNPRARSAKLRAAIRTNAD
AALDEASDGMNLPPLAELEKSR

Sequences:

>Translated_342_residues
MSLRTTPRSPAEPGHLPVMLAEVLTALAPADGEVIVDGTFGGGGYTTAILKAANCTVLGIDRDLDAIVRAEKMAQANPRL
VPLLGCFGDLDTLAEQAGHASVDGVVLDIGVSSFQIDQADRGFSFMKDGPLDMRMGGSGPSAADVVNTMSEKGLADVIFR
LGEEKNSRRIARVLVQRRVQAPFETTADLAAVVEEAVGGRRGARSHPATLTFQAIRMYVNDELGELARGLRAAERILKPG
GRLVVVTFHSLEDRLVKQWLRERAGAVPGGSRHMPLMSKGPAPAFELQENKAVQPSEKEVETNPRARSAKLRAAIRTNAD
AALDEASDGMNLPPLAELEKSR
>Mature_341_residues
SLRTTPRSPAEPGHLPVMLAEVLTALAPADGEVIVDGTFGGGGYTTAILKAANCTVLGIDRDLDAIVRAEKMAQANPRLV
PLLGCFGDLDTLAEQAGHASVDGVVLDIGVSSFQIDQADRGFSFMKDGPLDMRMGGSGPSAADVVNTMSEKGLADVIFRL
GEEKNSRRIARVLVQRRVQAPFETTADLAAVVEEAVGGRRGARSHPATLTFQAIRMYVNDELGELARGLRAAERILKPGG
RLVVVTFHSLEDRLVKQWLRERAGAVPGGSRHMPLMSKGPAPAFELQENKAVQPSEKEVETNPRARSAKLRAAIRTNADA
ALDEASDGMNLPPLAELEKSR

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=41.0714285714286, Blast_Score=236, Evalue=3e-62,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=42.3280423280423, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=42.4920127795527, Blast_Score=213, Evalue=2e-56,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=40.0602409638554, Blast_Score=204, Evalue=6e-53,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=40.0602409638554, Blast_Score=204, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_HYPNA (Q0BXT4)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761709.1
- ProteinModelPortal:   Q0BXT4
- SMR:   Q0BXT4
- STRING:   Q0BXT4
- GeneID:   4289135
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3032
- NMPDR:   fig|228405.5.peg.2907
- TIGR:   HNE_3032
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   HNEP81032:HNE_3032-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 36518; Mature: 36387

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: BINDING 61-61 BINDING 87-87 BINDING 108-108 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRTTPRSPAEPGHLPVMLAEVLTALAPADGEVIVDGTFGGGGYTTAILKAANCTVLGI
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEC
DRDLDAIVRAEKMAQANPRLVPLLGCFGDLDTLAEQAGHASVDGVVLDIGVSSFQIDQAD
CCCHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCCCCEEEEECCCEEEECCCC
RGFSFMKDGPLDMRMGGSGPSAADVVNTMSEKGLADVIFRLGEEKNSRRIARVLVQRRVQ
CCCCHHCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
APFETTADLAAVVEEAVGGRRGARSHPATLTFQAIRMYVNDELGELARGLRAAERILKPG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRLVVVTFHSLEDRLVKQWLRERAGAVPGGSRHMPLMSKGPAPAFELQENKAVQPSEKEV
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHH
ETNPRARSAKLRAAIRTNADAALDEASDGMNLPPLAELEKSR
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SLRTTPRSPAEPGHLPVMLAEVLTALAPADGEVIVDGTFGGGGYTTAILKAANCTVLGI
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEC
DRDLDAIVRAEKMAQANPRLVPLLGCFGDLDTLAEQAGHASVDGVVLDIGVSSFQIDQAD
CCCHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCCCCEEEEECCCEEEECCCC
RGFSFMKDGPLDMRMGGSGPSAADVVNTMSEKGLADVIFRLGEEKNSRRIARVLVQRRVQ
CCCCHHCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHC
APFETTADLAAVVEEAVGGRRGARSHPATLTFQAIRMYVNDELGELARGLRAAERILKPG
CCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRLVVVTFHSLEDRLVKQWLRERAGAVPGGSRHMPLMSKGPAPAFELQENKAVQPSEKEV
CCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHH
ETNPRARSAKLRAAIRTNADAALDEASDGMNLPPLAELEKSR
CCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA