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The map label for this gene is mltB [H]

Identifier: 114799549

GI number: 114799549

Start: 3498804

End: 3500108

Strand: Reverse

Name: mltB [H]

Synonym: HNE_3349

Alternate gene names: 114799549

Gene position: 3500108-3498804 (Counterclockwise)

Preceding gene: 114797278

Following gene: 114799859

Centisome position: 94.47

GC content: 64.98

Gene sequence:

>1305_bases
ATGGGGATTGCGGTCGTTTGCGGCGCGCTGGCATCGTGTGCGGCGGGGCCTCAGACGCCTTCGCCGAAGCCGCCGGTTAC
CGGAACACCCTCTCCAGCGCCGGAAACAGGCCCGATTGTCTACAAATCTTCCGGCCATGCCGCGATGGATGCCTGGCGGG
ATGATTTCTCGGCCCGCGCCCTGGCGGCCGGCAACGACCGGGCGATCGTGAAATCACTTCTGGAGAATATCAGCCCGATC
ACAATGTGGCTGGGATCGCAGGAAGTGGAAGTGCAGACCCAGACTGCGCCAAGCGATCAGGCCGAATTTGCCAAGCCGAT
CTGGGATTATCTCTCCACACCGCTGGGCAATACGCGGATCACGCAGGGCCAGCAGAAACTGGCTACCACCAGCGCCACGC
TTGCGGGCATCGAAGCCGAATATGGCGTCGACAGGCAGGCGCTGCTCGCCATCTGGGGCATGGAGACCAATTTCGGCGGT
TTCATCGGACGGGATGACGCGGCCAACGCGCTGGCCAACATGGCGGTCGAGGGCCGGCGGCGCAGCCTGGGCGAATCCGA
GCTGTTCGCCCTGATGAAAATCCTCAAGGATGGCGATGCCCGCCGGGGCGATCTGATCGCTGGCTGGGCCGGGGCCATGG
GCCAGACGCAGTTCATGCCGACCACCTATGTGGCCCATGCTGTCGACTTCGACGGGGACGGCCGCAAGGATGTCTGGAAA
AGTGAAGCCGACGCGCTGGCGTCAGCGGCCAATTATCTTGCCCGGTCGGGTTATGTGATGGGCCAGCCCTGGGGCATCGA
AGTGCTCACCCCGCAGGGGTTTGACTTCAGCCTGGCGGATGGCACGGAGCGGCGCATGGCGGCCTGGACGGCCGCCGGTC
TTTCCCCGATCCGGGGCGGCGCCTTCGAGACGGGCGGGGCGGATGTGGCAGAGCTCTGGCTGCCAGCGGGCGCAAGCGGA
CCGAAATACCTGCTGTTCAAGAACTTCAATGTCTACAAAACCTACAACCGCGCCGATTCCTATGCGCTGGCGGTGGGGCT
GTCGGGCGACATGATCGCCGGGAAAGGCGGGCCGGTCGCGTCCTGGCCGACGCATCTGCCGCCGCTGACGGTGGCTGACA
TTCGCGACCTGCAGACCAGCCTCAATGCCCTTGGCTATGATGCGGGTACGCCGGACGGCATTGCGGGCCGCCGGACCAAG
ACAGCGCTCCAGAACTTCCAGAAGGCGCGCGGTTTCTTGGCCGATGGCTATCCCACGCAGGAAATGCTGGCGGCCGTGAA
GGCGGGCGGGCCGTCGATCAATTGA

Upstream 100 bases:

>100_bases
AATGAATCCGGATAGCCGAGTCAAGAACGGTAACTGCGGCGAGAAGGCTGCTGAAAGGGTAAAGGCCATGATTATTACTG
GTTTGGCCCGTTCAAAATGG

Downstream 100 bases:

>100_bases
GCGCGTATCCGGGGCGGCGCCTAATCGTCGTCCCGGATTTCGCCCAGCCCGTCCTTCATGCCGAAACGGGCATAGACGAA
CATGGCGATCAGCATGCCGG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MGIAVVCGALASCAAGPQTPSPKPPVTGTPSPAPETGPIVYKSSGHAAMDAWRDDFSARALAAGNDRAIVKSLLENISPI
TMWLGSQEVEVQTQTAPSDQAEFAKPIWDYLSTPLGNTRITQGQQKLATTSATLAGIEAEYGVDRQALLAIWGMETNFGG
FIGRDDAANALANMAVEGRRRSLGESELFALMKILKDGDARRGDLIAGWAGAMGQTQFMPTTYVAHAVDFDGDGRKDVWK
SEADALASAANYLARSGYVMGQPWGIEVLTPQGFDFSLADGTERRMAAWTAAGLSPIRGGAFETGGADVAELWLPAGASG
PKYLLFKNFNVYKTYNRADSYALAVGLSGDMIAGKGGPVASWPTHLPPLTVADIRDLQTSLNALGYDAGTPDGIAGRRTK
TALQNFQKARGFLADGYPTQEMLAAVKAGGPSIN

Sequences:

>Translated_434_residues
MGIAVVCGALASCAAGPQTPSPKPPVTGTPSPAPETGPIVYKSSGHAAMDAWRDDFSARALAAGNDRAIVKSLLENISPI
TMWLGSQEVEVQTQTAPSDQAEFAKPIWDYLSTPLGNTRITQGQQKLATTSATLAGIEAEYGVDRQALLAIWGMETNFGG
FIGRDDAANALANMAVEGRRRSLGESELFALMKILKDGDARRGDLIAGWAGAMGQTQFMPTTYVAHAVDFDGDGRKDVWK
SEADALASAANYLARSGYVMGQPWGIEVLTPQGFDFSLADGTERRMAAWTAAGLSPIRGGAFETGGADVAELWLPAGASG
PKYLLFKNFNVYKTYNRADSYALAVGLSGDMIAGKGGPVASWPTHLPPLTVADIRDLQTSLNALGYDAGTPDGIAGRRTK
TALQNFQKARGFLADGYPTQEMLAAVKAGGPSIN
>Mature_433_residues
GIAVVCGALASCAAGPQTPSPKPPVTGTPSPAPETGPIVYKSSGHAAMDAWRDDFSARALAAGNDRAIVKSLLENISPIT
MWLGSQEVEVQTQTAPSDQAEFAKPIWDYLSTPLGNTRITQGQQKLATTSATLAGIEAEYGVDRQALLAIWGMETNFGGF
IGRDDAANALANMAVEGRRRSLGESELFALMKILKDGDARRGDLIAGWAGAMGQTQFMPTTYVAHAVDFDGDGRKDVWKS
EADALASAANYLARSGYVMGQPWGIEVLTPQGFDFSLADGTERRMAAWTAAGLSPIRGGAFETGGADVAELWLPAGASGP
KYLLFKNFNVYKTYNRADSYALAVGLSGDMIAGKGGPVASWPTHLPPLTVADIRDLQTSLNALGYDAGTPDGIAGRRTKT
ALQNFQKARGFLADGYPTQEMLAAVKAGGPSIN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=224, Percent_Identity=33.0357142857143, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45657; Mature: 45526

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIAVVCGALASCAAGPQTPSPKPPVTGTPSPAPETGPIVYKSSGHAAMDAWRDDFSARA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEE
LAAGNDRAIVKSLLENISPITMWLGSQEVEVQTQTAPSDQAEFAKPIWDYLSTPLGNTRI
EECCCCHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEE
TQGQQKLATTSATLAGIEAEYGVDRQALLAIWGMETNFGGFIGRDDAANALANMAVEGRR
CCCHHHHHHHHHHHHCCCHHHCCCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
RSLGESELFALMKILKDGDARRGDLIAGWAGAMGQTQFMPTTYVAHAVDFDGDGRKDVWK
HCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHEEEEECCCCCCCHHHHH
SEADALASAANYLARSGYVMGQPWGIEVLTPQGFDFSLADGTERRMAAWTAAGLSPIRGG
HHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCEEECCCCCHHHHHHHHHCCCCCCCCC
AFETGGADVAELWLPAGASGPKYLLFKNFNVYKTYNRADSYALAVGLSGDMIAGKGGPVA
CCCCCCCCHHHHEECCCCCCCEEEEEECCCEEEECCCCCCEEEEEECCCCEEECCCCCCC
SWPTHLPPLTVADIRDLQTSLNALGYDAGTPDGIAGRRTKTALQNFQKARGFLADGYPTQ
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
EMLAAVKAGGPSIN
HHHHHHHCCCCCCC
>Mature Secondary Structure 
GIAVVCGALASCAAGPQTPSPKPPVTGTPSPAPETGPIVYKSSGHAAMDAWRDDFSARA
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEE
LAAGNDRAIVKSLLENISPITMWLGSQEVEVQTQTAPSDQAEFAKPIWDYLSTPLGNTRI
EECCCCHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEE
TQGQQKLATTSATLAGIEAEYGVDRQALLAIWGMETNFGGFIGRDDAANALANMAVEGRR
CCCHHHHHHHHHHHHCCCHHHCCCHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
RSLGESELFALMKILKDGDARRGDLIAGWAGAMGQTQFMPTTYVAHAVDFDGDGRKDVWK
HCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHEEEEECCCCCCCHHHHH
SEADALASAANYLARSGYVMGQPWGIEVLTPQGFDFSLADGTERRMAAWTAAGLSPIRGG
HHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCEEECCCCCHHHHHHHHHCCCCCCCCC
AFETGGADVAELWLPAGASGPKYLLFKNFNVYKTYNRADSYALAVGLSGDMIAGKGGPVA
CCCCCCCCHHHHEECCCCCCCEEEEEECCCEEEECCCCCCEEEEEECCCCEEECCCCCCC
SWPTHLPPLTVADIRDLQTSLNALGYDAGTPDGIAGRRTKTALQNFQKARGFLADGYPTQ
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
EMLAAVKAGGPSIN
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]