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The map label for this gene is ycdT [H]

Identifier: 114799330

GI number: 114799330

Start: 2458920

End: 2460029

Strand: Direct

Name: ycdT [H]

Synonym: HNE_2358

Alternate gene names: 114799330

Gene position: 2458920-2460029 (Clockwise)

Preceding gene: 114797469

Following gene: 114797770

Centisome position: 66.37

GC content: 62.43

Gene sequence:

>1110_bases
ATGCTCACCTATCCGCCGCCGCGCTTTGAACAGCAAAAATCGGAAGTGCTTCAACGCTACCGGATTCTGAAAAGCGAAAC
GGTGCACGACGCAAGCCATATCGCGCGCACAGCGGCGCTGGCTCTGGGAACCCCCATCGTCATCGCCGCCCTGAACGAAC
GCTACCGCGCATGGTATCGCGCGGCTCATGGCGTCACAGACAATGACTTTCACAATCTCCAGGAATTCTGCGCTTACGCA
AACCTGTCCGACGATGCCTTCGCTGTAGCCGACGCTCGTCTGGAGCCCTATTTTGCCCGCGAGCCGGCAGTCATGGGCGC
CCCGAATGTCGTGTTCTTTGCCGGCGCGCCCCTCAGCGATCCCGATGGCAAGCGCTTCGGCACGCTCTGCCTGATAGGCA
ACCAGCCCCGCTCGCCGGCGCCCGGACAGATTGAACTCCTGAAAAGCATGGCGCATATCGTCAGCCAGGACATCTGCGTC
CACAGCGCCGGGCGCTACGCGGTCCAGGGCCTGATCAATGCCGAGGAAGAACGCTGCGCGCTTTACGATCTCGCCGTCAC
CGATCCTCTGACCAAAGCGCTCAACCACCGCGCCTTCTTCCGCTTTGCCGAGCGGGAAGTTCAGCGCGCCCAGCGCCACC
ACCACCCTGTCAGTGTGCTGATGTTTGACATCGACCATTTCAAGAAGGTGAATGATATTCACGGTCATGCGGCCGGCGAT
GAAGTCATAATCCGGCTCTCGCGTCTCGTCACCGAATGCGTGCGCGAGGCAGACCTGCTTGGCCGGCTGGGCGGCGAAGA
GTTTGGTCTGATCCTGCCTGAAACCAGTCTCGAGGCGGCTGTGACGCTGGCTAACCGGCTGCGCGAAGCCGTGAAGGATC
TCCGCTTCAAAAGCAGCGAAGGCACATTCGGCGTTACCATCAGCCTCGGCGTCAGCCAGCCCGGTGACACCGAGACCGAC
ATTGTCCCCGCCCTCGATCGGGCGGACAAGGCCCTCTACCGTGCCAAGCGCTTCGGCAGAGACCGGGCGGAGCTCATGCC
GCCGGCCATTGCCGAAAGCGCCTGCATCATTCAGCTGCCGCTAGCCCATCGGGCGAGCGACGCCGCCTGA

Upstream 100 bases:

>100_bases
GCCCGGATCGGTGAAATTTTATCGATCCGGGACAGTCCGGCTTCAGCACTCGTTATGCATAACAGCATGGTAACCAAGAT
GTCTGGGGGGACGTTTTTCC

Downstream 100 bases:

>100_bases
CAGCAGTGCGAGTTCGGCGCCGGATCAGCCGTCATCACCAGGCCGGCAGTCATCATCCCGCTTGCGGCGCAGCTCCTTGC
CCGAAACGGCATTGAACTTC

Product: GGDEF/GAF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MLTYPPPRFEQQKSEVLQRYRILKSETVHDASHIARTAALALGTPIVIAALNERYRAWYRAAHGVTDNDFHNLQEFCAYA
NLSDDAFAVADARLEPYFAREPAVMGAPNVVFFAGAPLSDPDGKRFGTLCLIGNQPRSPAPGQIELLKSMAHIVSQDICV
HSAGRYAVQGLINAEEERCALYDLAVTDPLTKALNHRAFFRFAEREVQRAQRHHHPVSVLMFDIDHFKKVNDIHGHAAGD
EVIIRLSRLVTECVREADLLGRLGGEEFGLILPETSLEAAVTLANRLREAVKDLRFKSSEGTFGVTISLGVSQPGDTETD
IVPALDRADKALYRAKRFGRDRAELMPPAIAESACIIQLPLAHRASDAA

Sequences:

>Translated_369_residues
MLTYPPPRFEQQKSEVLQRYRILKSETVHDASHIARTAALALGTPIVIAALNERYRAWYRAAHGVTDNDFHNLQEFCAYA
NLSDDAFAVADARLEPYFAREPAVMGAPNVVFFAGAPLSDPDGKRFGTLCLIGNQPRSPAPGQIELLKSMAHIVSQDICV
HSAGRYAVQGLINAEEERCALYDLAVTDPLTKALNHRAFFRFAEREVQRAQRHHHPVSVLMFDIDHFKKVNDIHGHAAGD
EVIIRLSRLVTECVREADLLGRLGGEEFGLILPETSLEAAVTLANRLREAVKDLRFKSSEGTFGVTISLGVSQPGDTETD
IVPALDRADKALYRAKRFGRDRAELMPPAIAESACIIQLPLAHRASDAA
>Mature_369_residues
MLTYPPPRFEQQKSEVLQRYRILKSETVHDASHIARTAALALGTPIVIAALNERYRAWYRAAHGVTDNDFHNLQEFCAYA
NLSDDAFAVADARLEPYFAREPAVMGAPNVVFFAGAPLSDPDGKRFGTLCLIGNQPRSPAPGQIELLKSMAHIVSQDICV
HSAGRYAVQGLINAEEERCALYDLAVTDPLTKALNHRAFFRFAEREVQRAQRHHHPVSVLMFDIDHFKKVNDIHGHAAGD
EVIIRLSRLVTECVREADLLGRLGGEEFGLILPETSLEAAVTLANRLREAVKDLRFKSSEGTFGVTISLGVSQPGDTETD
IVPALDRADKALYRAKRFGRDRAELMPPAIAESACIIQLPLAHRASDAA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=41.8181818181818, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1786584, Length=182, Percent_Identity=33.5164835164835, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081881, Length=156, Percent_Identity=39.7435897435897, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=40.8536585365854, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=36.5853658536585, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788381, Length=243, Percent_Identity=29.2181069958848, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=28.7292817679558, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081977, Length=241, Percent_Identity=26.1410788381743, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788085, Length=193, Percent_Identity=34.7150259067358, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787816, Length=126, Percent_Identity=35.7142857142857, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787541, Length=180, Percent_Identity=30, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=27.710843373494, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=26.5822784810127, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40741; Mature: 40741

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTYPPPRFEQQKSEVLQRYRILKSETVHDASHIARTAALALGTPIVIAALNERYRAWYR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
AAHGVTDNDFHNLQEFCAYANLSDDAFAVADARLEPYFAREPAVMGAPNVVFFAGAPLSD
HHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PDGKRFGTLCLIGNQPRSPAPGQIELLKSMAHIVSQDICVHSAGRYAVQGLINAEEERCA
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHE
LYDLAVTDPLTKALNHRAFFRFAEREVQRAQRHHHPVSVLMFDIDHFKKVNDIHGHAAGD
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCCCCCCH
EVIIRLSRLVTECVREADLLGRLGGEEFGLILPETSLEAAVTLANRLREAVKDLRFKSSE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
GTFGVTISLGVSQPGDTETDIVPALDRADKALYRAKRFGRDRAELMPPAIAESACIIQLP
CCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCEEEEEC
LAHRASDAA
CCCCCCCCC
>Mature Secondary Structure
MLTYPPPRFEQQKSEVLQRYRILKSETVHDASHIARTAALALGTPIVIAALNERYRAWYR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
AAHGVTDNDFHNLQEFCAYANLSDDAFAVADARLEPYFAREPAVMGAPNVVFFAGAPLSD
HHCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PDGKRFGTLCLIGNQPRSPAPGQIELLKSMAHIVSQDICVHSAGRYAVQGLINAEEERCA
CCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHE
LYDLAVTDPLTKALNHRAFFRFAEREVQRAQRHHHPVSVLMFDIDHFKKVNDIHGHAAGD
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHCCCCCCH
EVIIRLSRLVTECVREADLLGRLGGEEFGLILPETSLEAAVTLANRLREAVKDLRFKSSE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCC
GTFGVTISLGVSQPGDTETDIVPALDRADKALYRAKRFGRDRAELMPPAIAESACIIQLP
CCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCEEEEEC
LAHRASDAA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]