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The map label for this gene is sucB [H]

Identifier: 114799329

GI number: 114799329

Start: 309362

End: 310912

Strand: Direct

Name: sucB [H]

Synonym: HNE_0313

Alternate gene names: 114799329

Gene position: 309362-310912 (Clockwise)

Preceding gene: 114800323

Following gene: 114798543

Centisome position: 8.35

GC content: 62.86

Gene sequence:

>1551_bases
ATGACAGACATTGTTGTTCCCACACTCGGCGAAAGCGTTACCGAAGCCACCGTCGGCCAATGGCTGAAATCGGCCGGCGA
CGCGGTGAAGAAGGACGAAGTCCTCGTCGAACTTGAAACCGACAAGGTTTCCGTCGAGGTTTCGGCCTCTGAAGACGGCG
TCCTGTCGGAAATCGTGGCCAAGGAAGGCGACACTGTGGACATCGGCGCGCTGCTCGGCCGCCTGAACGCCAATGGCGCG
GCCAAGCCTGCACCCGCCAAGGAAGCCCCGAAGGCTGAAGCCAAGGCAGAGCCGAAAGCGGAACCCAAAGCGGCCGAAGC
CAAGCCGGCGGCCTCGTCCGGCGGCGGTGCGTCCACGGACGTAAAAGTCCCCGTGATGGGCGAAAGCGTTGCCGAAGGCA
CGATCGCCAACTTCGCCAAGAAGGTCGGCGAGAGCGTCAAGAAAGACGAGACCATCGCCGAGATCGAAACCGACAAGGTG
GCGCTGGAAGTTCCAGCCCCCGCAGACGGCGTGATCCTCGAATGGCTGGTCAAGGAAGGCGATTCCGTGACCCCCGGTTC
CGTCATTGCACGGATTGGCGCCTCGGGCGCGGCCCCTGCAAAAGCTGCCGAAGCGCCCGCGAAAGCCGAAGCGCCCAAAG
CCGCCGCCCAGCCAGCAGCCACCGGCGATCGCCCCGTTTCCCCATCGGTCCGCCGCATTTCCACCGAAGCCGGCGTCAGC
GCGTCTGATATTCCCGGCACCGGCCGCGATGGCCGCGCCACCAAGGCGGATGCCTTGGCCTACGTCAACCAACCGAAAGC
AAGCGCCTCGACCATGCCCGACACCGCCGCAAAACCACCGCGCGAAACCGGCCCCCGTGAAGAACGTGTACGGATGACGC
GTCTGCGTCAGACCATCGCCCGCCGCCTGAAGGAAGCTCAGGACACGGCCGCGATGCTGACCACGTTCAACGATGTCGAC
ATGTCGGCGATCATGGATCTGCGCAAGCAGCACCAGGACGCCTTCGTCGCCAAGCACGGCATCAAGCTCGGCTTCATGAG
CTTCTTCGTGAAGGCGTGCGTCAACGCGCTGAAGGAAGTTCCTGCCGTCAACGCCGAGATCGATGGCCAGGACGTCATCT
ACAAGAACTATTATGACATCGGCGTGGCCGTCGGCACCGAGAAGGGCCTTGTTGTGCCCGTCGTGCGCGACGCAGACGAT
CTCTCGCTGGCCGGCATCGAGAAAGCCATCGCGGCGCTGGGCAAGAAAGCCCGTGACGGCGATCTCACCATCGGCGACAT
GCAGGGCGGCACCTTCACCATCTCGAACGGCGGCATCTATGGCTCGCTGATGTCGACCCCGATCCTCAATCCGCCGCAGT
CCGGCGTGCTCGGTATGCACCGTATAGAGGACAAGCCGATCGTCCGTAACGGCCAGATCGTCATCAAGCCGATGATGTAT
CTTGCGCTGAGCTATGACCACCGCATCGTTGACGGCAAGGAAGCGGTTACCTTCCTCGTTCGTGTCAAGGAAGCGCTGGA
AGATCCTGAGCGGATGCTGCTCGAAGTCTAA

Upstream 100 bases:

>100_bases
CCGAACAGGCCAACCTTCTCAAGACAGCCCTCTCGCCCGACCCGGTCGATGACCGGATCGTTTCTCCCTGATAATCCATA
AGAAGAGACGGACACGACAC

Downstream 100 bases:

>100_bases
CTCTGCTTCCGGGTGAACCATCCCCGGATCATCAGAGACTGAAAGAACGGAACGGGCTGATCGAAGCGGGCTGCTTTGAT
CAGCTCTTCCACTTTCTGGG

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTDIVVPTLGESVTEATVGQWLKSAGDAVKKDEVLVELETDKVSVEVSASEDGVLSEIVAKEGDTVDIGALLGRLNANGA
AKPAPAKEAPKAEAKAEPKAEPKAAEAKPAASSGGGASTDVKVPVMGESVAEGTIANFAKKVGESVKKDETIAEIETDKV
ALEVPAPADGVILEWLVKEGDSVTPGSVIARIGASGAAPAKAAEAPAKAEAPKAAAQPAATGDRPVSPSVRRISTEAGVS
ASDIPGTGRDGRATKADALAYVNQPKASASTMPDTAAKPPRETGPREERVRMTRLRQTIARRLKEAQDTAAMLTTFNDVD
MSAIMDLRKQHQDAFVAKHGIKLGFMSFFVKACVNALKEVPAVNAEIDGQDVIYKNYYDIGVAVGTEKGLVVPVVRDADD
LSLAGIEKAIAALGKKARDGDLTIGDMQGGTFTISNGGIYGSLMSTPILNPPQSGVLGMHRIEDKPIVRNGQIVIKPMMY
LALSYDHRIVDGKEAVTFLVRVKEALEDPERMLLEV

Sequences:

>Translated_516_residues
MTDIVVPTLGESVTEATVGQWLKSAGDAVKKDEVLVELETDKVSVEVSASEDGVLSEIVAKEGDTVDIGALLGRLNANGA
AKPAPAKEAPKAEAKAEPKAEPKAAEAKPAASSGGGASTDVKVPVMGESVAEGTIANFAKKVGESVKKDETIAEIETDKV
ALEVPAPADGVILEWLVKEGDSVTPGSVIARIGASGAAPAKAAEAPAKAEAPKAAAQPAATGDRPVSPSVRRISTEAGVS
ASDIPGTGRDGRATKADALAYVNQPKASASTMPDTAAKPPRETGPREERVRMTRLRQTIARRLKEAQDTAAMLTTFNDVD
MSAIMDLRKQHQDAFVAKHGIKLGFMSFFVKACVNALKEVPAVNAEIDGQDVIYKNYYDIGVAVGTEKGLVVPVVRDADD
LSLAGIEKAIAALGKKARDGDLTIGDMQGGTFTISNGGIYGSLMSTPILNPPQSGVLGMHRIEDKPIVRNGQIVIKPMMY
LALSYDHRIVDGKEAVTFLVRVKEALEDPERMLLEV
>Mature_515_residues
TDIVVPTLGESVTEATVGQWLKSAGDAVKKDEVLVELETDKVSVEVSASEDGVLSEIVAKEGDTVDIGALLGRLNANGAA
KPAPAKEAPKAEAKAEPKAEPKAAEAKPAASSGGGASTDVKVPVMGESVAEGTIANFAKKVGESVKKDETIAEIETDKVA
LEVPAPADGVILEWLVKEGDSVTPGSVIARIGASGAAPAKAAEAPAKAEAPKAAAQPAATGDRPVSPSVRRISTEAGVSA
SDIPGTGRDGRATKADALAYVNQPKASASTMPDTAAKPPRETGPREERVRMTRLRQTIARRLKEAQDTAAMLTTFNDVDM
SAIMDLRKQHQDAFVAKHGIKLGFMSFFVKACVNALKEVPAVNAEIDGQDVIYKNYYDIGVAVGTEKGLVVPVVRDADDL
SLAGIEKAIAALGKKARDGDLTIGDMQGGTFTISNGGIYGSLMSTPILNPPQSGVLGMHRIEDKPIVRNGQIVIKPMMYL
ALSYDHRIVDGKEAVTFLVRVKEALEDPERMLLEV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=57.7586206896552, Blast_Score=297, Evalue=2e-80,
Organism=Homo sapiens, GI31711992, Length=563, Percent_Identity=27.708703374778, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI110671329, Length=424, Percent_Identity=27.8301886792453, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI203098816, Length=475, Percent_Identity=30.1052631578947, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=29.6703296703297, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=36.0248447204969, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1786946, Length=404, Percent_Identity=51.2376237623762, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1786305, Length=549, Percent_Identity=30.9653916211293, Blast_Score=204, Evalue=8e-54,
Organism=Caenorhabditis elegans, GI25146366, Length=405, Percent_Identity=45.679012345679, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17560088, Length=438, Percent_Identity=29.4520547945205, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=425, Percent_Identity=28.4705882352941, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=319, Percent_Identity=28.8401253918495, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320352, Length=409, Percent_Identity=46.6992665036675, Blast_Score=357, Evalue=3e-99,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=30.5309734513274, Blast_Score=140, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=60.0896860986547, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI18859875, Length=421, Percent_Identity=27.790973871734, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129315, Length=239, Percent_Identity=30.9623430962343, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24582497, Length=239, Percent_Identity=30.9623430962343, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 54001; Mature: 53870

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIVVPTLGESVTEATVGQWLKSAGDAVKKDEVLVELETDKVSVEVSASEDGVLSEIVA
CCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHH
KEGDTVDIGALLGRLNANGAAKPAPAKEAPKAEAKAEPKAEPKAAEAKPAASSGGGASTD
CCCCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VKVPVMGESVAEGTIANFAKKVGESVKKDETIAEIETDKVALEVPAPADGVILEWLVKEG
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHCC
DSVTPGSVIARIGASGAAPAKAAEAPAKAEAPKAAAQPAATGDRPVSPSVRRISTEAGVS
CCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHCCCC
ASDIPGTGRDGRATKADALAYVNQPKASASTMPDTAAKPPRETGPREERVRMTRLRQTIA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RRLKEAQDTAAMLTTFNDVDMSAIMDLRKQHQDAFVAKHGIKLGFMSFFVKACVNALKEV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
PAVNAEIDGQDVIYKNYYDIGVAVGTEKGLVVPVVRDADDLSLAGIEKAIAALGKKARDG
CCCCCCCCCCCEEEECEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
DLTIGDMQGGTFTISNGGIYGSLMSTPILNPPQSGVLGMHRIEDKPIVRNGQIVIKPMMY
CEEEEECCCCEEEEECCCEEEHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCEEEEEHHH
LALSYDHRIVDGKEAVTFLVRVKEALEDPERMLLEV
HHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
TDIVVPTLGESVTEATVGQWLKSAGDAVKKDEVLVELETDKVSVEVSASEDGVLSEIVA
CCEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHH
KEGDTVDIGALLGRLNANGAAKPAPAKEAPKAEAKAEPKAEPKAAEAKPAASSGGGASTD
CCCCEEEHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCE
VKVPVMGESVAEGTIANFAKKVGESVKKDETIAEIETDKVALEVPAPADGVILEWLVKEG
EEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHCC
DSVTPGSVIARIGASGAAPAKAAEAPAKAEAPKAAAQPAATGDRPVSPSVRRISTEAGVS
CCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHCCCC
ASDIPGTGRDGRATKADALAYVNQPKASASTMPDTAAKPPRETGPREERVRMTRLRQTIA
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
RRLKEAQDTAAMLTTFNDVDMSAIMDLRKQHQDAFVAKHGIKLGFMSFFVKACVNALKEV
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
PAVNAEIDGQDVIYKNYYDIGVAVGTEKGLVVPVVRDADDLSLAGIEKAIAALGKKARDG
CCCCCCCCCCCEEEECEEEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCC
DLTIGDMQGGTFTISNGGIYGSLMSTPILNPPQSGVLGMHRIEDKPIVRNGQIVIKPMMY
CEEEEECCCCEEEEECCCEEEHHHCCCCCCCCCCCCCCHHHCCCCCEEECCCEEEEEHHH
LALSYDHRIVDGKEAVTFLVRVKEALEDPERMLLEV
HHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]