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The map label for this gene is sspA [C]

Identifier: 114799214

GI number: 114799214

Start: 3280133

End: 3280810

Strand: Reverse

Name: sspA [C]

Synonym: HNE_3104

Alternate gene names: 114799214

Gene position: 3280810-3280133 (Counterclockwise)

Preceding gene: 114799217

Following gene: 114799749

Centisome position: 88.55

GC content: 66.52

Gene sequence:

>678_bases
ATGCTGCGACTGTATCACTGGCCCCTCGATCCTGCTGGCCGCATGGTCCGCCTCGTCCTTGCCGAAAAAGGCGAGCCCTT
CGAGGCCGTGTCCTCCCGTCCCTGGGCGCCGGAACTGGAGATCGCCTCGATCGCGCCCGGCGCGGTGGCCCCGGCCTTGG
TGTCTACACACGGCACCACGCCGCGTTTTGCCGCCAGCGGCACGCGCGCCATCTGTGAGCATCTCGAAGAAATCCGCCCG
GTCCCGTCGCTGCTCCCGGATGACCCAACCGAACGCGCCGAGGCCCGCCGCCTCTGGTCATGGGTCGAGGCGGGCATGGA
GGAAGTCACCGACACCCTCCTGTCGGAACGTGTCATCCAGTGGACGCACCGTGGCCGTCAGCCTGATTCGGAGAAGCTGC
GCCGCGGGGCCCATGCTCTGCGTGGCCGGCTCACCTTCCTCAATGCCCTCGCCGAGCAGCGCGGCAACCTCGCCGGCCGC
CACCTTTCGCTGGCAGACTTCGCCGCCGCCGCCCATCTCTCCGCCTACGACTATTTCGGCGATGTGCAGTGGGAAGCCGT
GCCAGACCTCAAAGCCTGGTATATGCGGATAAAGTCGCGTCCGAGTTTCCGGCCGATCCTTGCTGACCGCCTGGAGGCTG
CCCGTCCCGTATCCTATTATGCAGAGCTCGATTTCTGA

Upstream 100 bases:

>100_bases
ACCGACGCCGTGCGACAATTCATGGGTCGTTAGCAAAAAAGCCGGAGGAAACCGCACGGGGTCCACAGAACGTTTACGCA
TGTTATCCACAGTATAATTC

Downstream 100 bases:

>100_bases
CCCCGCAGCCCGGCTAAAAGAACGGGCCCTCGCCCTCGGCTTTGACGCCGCCGGCATCGCGCGCGCCGATGAGGCATGGC
CTGCCGCCGAGCGCCTGAAA

Product: glutathione S-transferase

Products: HX; R-S-glutathione

Alternate protein names: Glutathione S-Transferase Domain-Containing Protein; Glutathione S-Transferase Family Protein; Glutathione S-Transferase Domain Protein; Glutathione S-Transferase-Like; Glutathione S-Transferase-Like Protein; Glutathione S-Transferase Protein; Unkown Function; Glutathione S-Transferase C-Terminal Domain Protein; Glutathione S-Transferase Domain; Glutathione S- Transferase N-Terminal

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MLRLYHWPLDPAGRMVRLVLAEKGEPFEAVSSRPWAPELEIASIAPGAVAPALVSTHGTTPRFAASGTRAICEHLEEIRP
VPSLLPDDPTERAEARRLWSWVEAGMEEVTDTLLSERVIQWTHRGRQPDSEKLRRGAHALRGRLTFLNALAEQRGNLAGR
HLSLADFAAAAHLSAYDYFGDVQWEAVPDLKAWYMRIKSRPSFRPILADRLEAARPVSYYAELDF

Sequences:

>Translated_225_residues
MLRLYHWPLDPAGRMVRLVLAEKGEPFEAVSSRPWAPELEIASIAPGAVAPALVSTHGTTPRFAASGTRAICEHLEEIRP
VPSLLPDDPTERAEARRLWSWVEAGMEEVTDTLLSERVIQWTHRGRQPDSEKLRRGAHALRGRLTFLNALAEQRGNLAGR
HLSLADFAAAAHLSAYDYFGDVQWEAVPDLKAWYMRIKSRPSFRPILADRLEAARPVSYYAELDF
>Mature_225_residues
MLRLYHWPLDPAGRMVRLVLAEKGEPFEAVSSRPWAPELEIASIAPGAVAPALVSTHGTTPRFAASGTRAICEHLEEIRP
VPSLLPDDPTERAEARRLWSWVEAGMEEVTDTLLSERVIQWTHRGRQPDSEKLRRGAHALRGRLTFLNALAEQRGNLAGR
HLSLADFAAAAHLSAYDYFGDVQWEAVPDLKAWYMRIKSRPSFRPILADRLEAARPVSYYAELDF

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.5.1.18

Molecular weight: Translated: 25303; Mature: 25303

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLYHWPLDPAGRMVRLVLAEKGEPFEAVSSRPWAPELEIASIAPGAVAPALVSTHGTT
CCEEEECCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHCCCCCHHHHHHHHCCCCC
PRFAASGTRAICEHLEEIRPVPSLLPDDPTERAEARRLWSWVEAGMEEVTDTLLSERVIQ
CCHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTHRGRQPDSEKLRRGAHALRGRLTFLNALAEQRGNLAGRHLSLADFAAAAHLSAYDYFG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
DVQWEAVPDLKAWYMRIKSRPSFRPILADRLEAARPVSYYAELDF
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHEECCC
>Mature Secondary Structure
MLRLYHWPLDPAGRMVRLVLAEKGEPFEAVSSRPWAPELEIASIAPGAVAPALVSTHGTT
CCEEEECCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHCCCCCHHHHHHHHCCCCC
PRFAASGTRAICEHLEEIRPVPSLLPDDPTERAEARRLWSWVEAGMEEVTDTLLSERVIQ
CCHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WTHRGRQPDSEKLRRGAHALRGRLTFLNALAEQRGNLAGRHLSLADFAAAAHLSAYDYFG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
DVQWEAVPDLKAWYMRIKSRPSFRPILADRLEAARPVSYYAELDF
CCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA