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The map label for this gene is folD

Identifier: 114799083

GI number: 114799083

Start: 3419300

End: 3420187

Strand: Reverse

Name: folD

Synonym: HNE_3253

Alternate gene names: 114799083

Gene position: 3420187-3419300 (Counterclockwise)

Preceding gene: 114798070

Following gene: 114798634

Centisome position: 92.31

GC content: 64.53

Gene sequence:

>888_bases
ATGACAGCGACAAGACTTGATGGCAATGCAGTGGCGGCGGATGTGCGATCTGCTGTGGCCGCTGCAGTTTCGGCCCTTCC
GGGGCAGCCGTGCCTGGCCGTTGTGCTGGTCGGCGAAGACCCTGCCAGCGAGGTCTATGTCCGCAACAAGGTGAAGATGA
CCGAGGCCGCCGGCATGGTCTCCATCCACCATCGTTTGCCCGCAGATACGGGCCAGGCCGAGGTTGAGCGCCTCATCGAA
AGCCTCAACGCTGACCCGGAAGTCGACGGCATCCTTCTCCAGCTGCCCCTGCCAAAGGGCCTCGACGCCGACGCGGCAAT
TGAAAAAATCAATCCGGACAAGGATGTAGACGGTCTGACGGAAGTGTCCGCCGGCCGTCTCAGCCTCGGCAAGCCGGGCC
TGCGCCCGTGCACCCCCGTTGGCTCCGTCATCCTGGCCAAACGCGCCTTAGGCCCTGATCTTTCGGGGAAAAATGTCGTC
GTCATCGGCCGCTCGATCCTGGTCGGCAAGCCCGCCGCCCTGCTGTTCCTTGCCGAAAACTGCACCGTCACCATCGCCCA
TAGCAAGACGGCAGACCTGGCCAACGTGTGCCGGTCTGCAGACATCCTGGTGCCAGCGGTTGGCCGGCCGGAGATGGTGC
GGGGCGACTGGGTGAAGCCCGGTGCTGCCGTGATCGATGTGGGCATCAACCGCATTCCGGCGCCTGAAAAAGGTGAAGGA
AAAACAAGGCTTGTGGGCGACGCGCATTATGAAAGCTGCGCGGAAGTGGCCGGGTTCATCACGCCGGTGCCGGGCGGGGT
GGGGCCGATGACGATTGCCTGTCTCTTGCGCAATACGGTGCTGGCGGCCTGCGCGCGGCGCGGCTGGACCGTGCCGGAGG
GGCTGTGA

Upstream 100 bases:

>100_bases
TCTGCCGTCAGGGTGGTGACGGGCGGCAAGAACCGGCTAAAAAGCCTCGAAATCGAGGGGCCGCCCGACCTCGCGGCCCG
TATAAGCGGATTGGCGGGCG

Downstream 100 bases:

>100_bases
GCCAGAGCCATACGCCGCCAGAATCAGGACAATTCCGGACACTTTGGGACAAACAGGGCGGTCTCTGCGCCCTTTGCGGA
CAACCGATGCCACGCCACCG

Product: putative FolD bifunctional protein

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTATRLDGNAVAADVRSAVAAAVSALPGQPCLAVVLVGEDPASEVYVRNKVKMTEAAGMVSIHHRLPADTGQAEVERLIE
SLNADPEVDGILLQLPLPKGLDADAAIEKINPDKDVDGLTEVSAGRLSLGKPGLRPCTPVGSVILAKRALGPDLSGKNVV
VIGRSILVGKPAALLFLAENCTVTIAHSKTADLANVCRSADILVPAVGRPEMVRGDWVKPGAAVIDVGINRIPAPEKGEG
KTRLVGDAHYESCAEVAGFITPVPGGVGPMTIACLLRNTVLAACARRGWTVPEGL

Sequences:

>Translated_295_residues
MTATRLDGNAVAADVRSAVAAAVSALPGQPCLAVVLVGEDPASEVYVRNKVKMTEAAGMVSIHHRLPADTGQAEVERLIE
SLNADPEVDGILLQLPLPKGLDADAAIEKINPDKDVDGLTEVSAGRLSLGKPGLRPCTPVGSVILAKRALGPDLSGKNVV
VIGRSILVGKPAALLFLAENCTVTIAHSKTADLANVCRSADILVPAVGRPEMVRGDWVKPGAAVIDVGINRIPAPEKGEG
KTRLVGDAHYESCAEVAGFITPVPGGVGPMTIACLLRNTVLAACARRGWTVPEGL
>Mature_294_residues
TATRLDGNAVAADVRSAVAAAVSALPGQPCLAVVLVGEDPASEVYVRNKVKMTEAAGMVSIHHRLPADTGQAEVERLIES
LNADPEVDGILLQLPLPKGLDADAAIEKINPDKDVDGLTEVSAGRLSLGKPGLRPCTPVGSVILAKRALGPDLSGKNVVV
IGRSILVGKPAALLFLAENCTVTIAHSKTADLANVCRSADILVPAVGRPEMVRGDWVKPGAAVIDVGINRIPAPEKGEGK
TRLVGDAHYESCAEVAGFITPVPGGVGPMTIACLLRNTVLAACARRGWTVPEGL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=287, Percent_Identity=44.5993031358885, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI222418558, Length=283, Percent_Identity=44.8763250883392, Blast_Score=207, Evalue=7e-54,
Organism=Homo sapiens, GI94721354, Length=285, Percent_Identity=43.1578947368421, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI36796743, Length=199, Percent_Identity=29.1457286432161, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1786741, Length=288, Percent_Identity=49.3055555555556, Blast_Score=248, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17568735, Length=284, Percent_Identity=46.830985915493, Blast_Score=227, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=297, Percent_Identity=41.4141414141414, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.2818791946309, Blast_Score=217, Evalue=2e-57,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=45.5479452054795, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=45.5479452054795, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=45.1048951048951, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=45.1048951048951, Blast_Score=223, Evalue=8e-59,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=45.1048951048951, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=45.1048951048951, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_HYPNA (Q0BX67)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761926.1
- ProteinModelPortal:   Q0BX67
- SMR:   Q0BX67
- STRING:   Q0BX67
- GeneID:   4287737
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3253
- NMPDR:   fig|228405.5.peg.3114
- TIGR:   HNE_3253
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CTPLGCI
- ProtClustDB:   CLSK777530
- BioCyc:   HNEP81032:HNE_3253-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30437; Mature: 30305

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATRLDGNAVAADVRSAVAAAVSALPGQPCLAVVLVGEDPASEVYVRNKVKMTEAAGMV
CCCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCEEEHHHCCEE
SIHHRLPADTGQAEVERLIESLNADPEVDGILLQLPLPKGLDADAAIEKINPDKDVDGLT
EEHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCHH
EVSAGRLSLGKPGLRPCTPVGSVILAKRALGPDLSGKNVVVIGRSILVGKPAALLFLAEN
HHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECEEEECCCEEEEEEECC
CTVTIAHSKTADLANVCRSADILVPAVGRPEMVRGDWVKPGAAVIDVGINRIPAPEKGEG
CEEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
KTRLVGDAHYESCAEVAGFITPVPGGVGPMTIACLLRNTVLAACARRGWTVPEGL
CEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TATRLDGNAVAADVRSAVAAAVSALPGQPCLAVVLVGEDPASEVYVRNKVKMTEAAGMV
CCEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCEEEHHHCCEE
SIHHRLPADTGQAEVERLIESLNADPEVDGILLQLPLPKGLDADAAIEKINPDKDVDGLT
EEHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCHH
EVSAGRLSLGKPGLRPCTPVGSVILAKRALGPDLSGKNVVVIGRSILVGKPAALLFLAEN
HHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECEEEECCCEEEEEEECC
CTVTIAHSKTADLANVCRSADILVPAVGRPEMVRGDWVKPGAAVIDVGINRIPAPEKGEG
CEEEEEECCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCC
KTRLVGDAHYESCAEVAGFITPVPGGVGPMTIACLLRNTVLAACARRGWTVPEGL
CEEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA