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The map label for this gene is dagA [H]

Identifier: 114798937

GI number: 114798937

Start: 2437466

End: 2438887

Strand: Direct

Name: dagA [H]

Synonym: HNE_2334

Alternate gene names: 114798937

Gene position: 2437466-2438887 (Clockwise)

Preceding gene: 114799705

Following gene: 114797486

Centisome position: 65.79

GC content: 65.4

Gene sequence:

>1422_bases
ATGGACGCTCTGAGAAACGCCCTGACCGCTTTCGGTGACTGGCTCATCGGCATGGTTTTCACCGCCGTCCCCATCTTCGG
GGTTGAGGTAAACCTGATCGTCCTCTGGCTCTTCGCCGGCATGGTGTTCTTCACGCTGCGTCTTGGCTTCCTCAATCTGC
GAGGCTTCGGCACCGCCGTCTCCATCCTCCGGGGACGGAATTATGACCCCACCGCGCCCGGCGAAATGACACCCTTCCAG
GCTCTTTCCACCGCGCTCTCGGGCACAGTCGGCCTCGGTAACATCGCCGGCGTCGCCATCGCCATCGCCATGGGCGGCCC
CGGCGCCGCCTTCTGGATGGTCGTCGTCGGCTTCTTCGCCATGACGCTGAAATTCGCCGAAGTCGTCCTCGCCGTCCGCT
ATCGCACGATAGACGCAGACGGCCGCGTCTCCGGTGGCCCGATGTATTATCTCTCCCGCGGCCTCGCCGAGAAAGGCCTT
CCGCGCTTGGGTAAAGTCCTCGGTACGCTCTACGCCGTCTTCGCCCTCGTCGCCTTCATCCAGATCGTCCAGGTCAACCA
GTCCTATTCCCAGGTCAGCACCGTTCTGGGCCTTGAGCAGAACACCGGCGCGGCGCTGGGCTATGGCATCCTCATCGCCG
GCCTCGCCGCCCTCGTCCTCCTCGGCGGCGCCGCCTCCCTCGCCCGCATCACGTCAAAGCTCACGCCCCTGATGTGCGGC
CTCTATCTTACCGGCGCGATCATTGTCCTCGGCGTCAACGTCACCGCCATTCCCGGCGCGCTGGCCACCATCGCCGTCCA
GGCCTTCACGCCAGACGCCGCCGTCGGCGGCCTCGTCGGCGCCTTCGTGGCGGGCATGCGCCGCGCCGTCTTCTCCAATG
AAGCGGGCATCGGCGCCGCCTCCATCGCCCACGCCGCCGCCAAAACGCGCTATCCGGCGTCCGAAGGCTTCGTCGCCCTG
CTCGAGCCCTTTATCGACACCGTCATCGTCTGCTCGGCCACCGCCCTCGTCATCGTCACCACCGGCGTCTGGAATGATGG
CCACACCGACATCGCCATGACGTCAGCCGCCTTCGGCACGGTCTCCTCCTGGTTCCCCGGAATCCTGGCCGTCGCCGTCT
GCCTCTTCGCCTTCTCCACCATCCTGGCGGTCGGCTATTACGGCCTCCAGATCTGCGCCTACCTCTTCGGCCCCCACCCC
CTCATCCGGCGCATCTACCTTACCCTCTTCTGCCTCCTCCTGCCGCTCGGCACGATTATGGATGTCACCACCGTCCTCAA
CATCATCGACAGCCTCTTCTTCCTGCTATCGGTCCCAAACCTGATCGGCCTCTACATCCTCTCCGGCGTCGTCGCCGCAG
AGCTACGCACCTTCCGCGAAGCAGAGCGCCAAAGAGTGTCCGCAGAGCGCCGCGCCGGGTAA

Upstream 100 bases:

>100_bases
CCGGCAGCCCGTTCAACCTCCTGCGCTGGTCCTCCCCGCGCACGGTGAAGGAAACTTTCGTGCCGCCCGTCTCCCACCTC
TATCCCCATATGGCAGGCTG

Downstream 100 bases:

>100_bases
TTTCTGTCCGGCTTTGTCCAGATTTGTCCAACTCTGCCCCCCCTGCCGTTGGCGTTTTGTTGAAGCGTGGTTGGAGTTTT
CCGCCAAACACGTCAACGGC

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MDALRNALTAFGDWLIGMVFTAVPIFGVEVNLIVLWLFAGMVFFTLRLGFLNLRGFGTAVSILRGRNYDPTAPGEMTPFQ
ALSTALSGTVGLGNIAGVAIAIAMGGPGAAFWMVVVGFFAMTLKFAEVVLAVRYRTIDADGRVSGGPMYYLSRGLAEKGL
PRLGKVLGTLYAVFALVAFIQIVQVNQSYSQVSTVLGLEQNTGAALGYGILIAGLAALVLLGGAASLARITSKLTPLMCG
LYLTGAIIVLGVNVTAIPGALATIAVQAFTPDAAVGGLVGAFVAGMRRAVFSNEAGIGAASIAHAAAKTRYPASEGFVAL
LEPFIDTVIVCSATALVIVTTGVWNDGHTDIAMTSAAFGTVSSWFPGILAVAVCLFAFSTILAVGYYGLQICAYLFGPHP
LIRRIYLTLFCLLLPLGTIMDVTTVLNIIDSLFFLLSVPNLIGLYILSGVVAAELRTFREAERQRVSAERRAG

Sequences:

>Translated_473_residues
MDALRNALTAFGDWLIGMVFTAVPIFGVEVNLIVLWLFAGMVFFTLRLGFLNLRGFGTAVSILRGRNYDPTAPGEMTPFQ
ALSTALSGTVGLGNIAGVAIAIAMGGPGAAFWMVVVGFFAMTLKFAEVVLAVRYRTIDADGRVSGGPMYYLSRGLAEKGL
PRLGKVLGTLYAVFALVAFIQIVQVNQSYSQVSTVLGLEQNTGAALGYGILIAGLAALVLLGGAASLARITSKLTPLMCG
LYLTGAIIVLGVNVTAIPGALATIAVQAFTPDAAVGGLVGAFVAGMRRAVFSNEAGIGAASIAHAAAKTRYPASEGFVAL
LEPFIDTVIVCSATALVIVTTGVWNDGHTDIAMTSAAFGTVSSWFPGILAVAVCLFAFSTILAVGYYGLQICAYLFGPHP
LIRRIYLTLFCLLLPLGTIMDVTTVLNIIDSLFFLLSVPNLIGLYILSGVVAAELRTFREAERQRVSAERRAG
>Mature_473_residues
MDALRNALTAFGDWLIGMVFTAVPIFGVEVNLIVLWLFAGMVFFTLRLGFLNLRGFGTAVSILRGRNYDPTAPGEMTPFQ
ALSTALSGTVGLGNIAGVAIAIAMGGPGAAFWMVVVGFFAMTLKFAEVVLAVRYRTIDADGRVSGGPMYYLSRGLAEKGL
PRLGKVLGTLYAVFALVAFIQIVQVNQSYSQVSTVLGLEQNTGAALGYGILIAGLAALVLLGGAASLARITSKLTPLMCG
LYLTGAIIVLGVNVTAIPGALATIAVQAFTPDAAVGGLVGAFVAGMRRAVFSNEAGIGAASIAHAAAKTRYPASEGFVAL
LEPFIDTVIVCSATALVIVTTGVWNDGHTDIAMTSAAFGTVSSWFPGILAVAVCLFAFSTILAVGYYGLQICAYLFGPHP
LIRRIYLTLFCLLLPLGTIMDVTTVLNIIDSLFFLLSVPNLIGLYILSGVVAAELRTFREAERQRVSAERRAG

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=436, Percent_Identity=32.1100917431193, Blast_Score=170, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 49545; Mature: 49545

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALRNALTAFGDWLIGMVFTAVPIFGVEVNLIVLWLFAGMVFFTLRLGFLNLRGFGTAV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
SILRGRNYDPTAPGEMTPFQALSTALSGTVGLGNIAGVAIAIAMGGPGAAFWMVVVGFFA
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHEEEECCCHHHHHHHHHHHHH
MTLKFAEVVLAVRYRTIDADGRVSGGPMYYLSRGLAEKGLPRLGKVLGTLYAVFALVAFI
HHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QIVQVNQSYSQVSTVLGLEQNTGAALGYGILIAGLAALVLLGGAASLARITSKLTPLMCG
HHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LYLTGAIIVLGVNVTAIPGALATIAVQAFTPDAAVGGLVGAFVAGMRRAVFSNEAGIGAA
HHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SIAHAAAKTRYPASEGFVALLEPFIDTVIVCSATALVIVTTGVWNDGHTDIAMTSAAFGT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHH
VSSWFPGILAVAVCLFAFSTILAVGYYGLQICAYLFGPHPLIRRIYLTLFCLLLPLGTIM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
DVTTVLNIIDSLFFLLSVPNLIGLYILSGVVAAELRTFREAERQRVSAERRAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]