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The map label for this gene is truA

Identifier: 114798579

GI number: 114798579

Start: 507919

End: 508662

Strand: Direct

Name: truA

Synonym: HNE_0515

Alternate gene names: 114798579

Gene position: 507919-508662 (Clockwise)

Preceding gene: 114798503

Following gene: 114800028

Centisome position: 13.71

GC content: 65.46

Gene sequence:

>744_bases
ATGCCCCGTTACAGACTCCTCATCGAATATAATGGCGGGCCCTATCAGGGCTGGATGCGCTTGCCCGGCCTGCAGACCGT
GCAGGGCGCGCTGGAGGCCGCCGCGGCCCAGCTCGATGGCGGGCCGGTGGAGGTCACCGGGGCCGGGCGCACCGATGCCG
GCGTGCATGCCACGGGGCAGGTGGCGCATATGGATTTGCGCGTCGACCGGCCCAACAAGGTGGCTGACGCCATGAACTAT
CATCTGCGCCCCCATCCCATCGCTGTGTTGAAAGCCGACAGGGTGGAGGAGGATTTCCATGCCCGCTTTTCGGCCATCGC
GCGTCACTACCGCTACATCGTCATCAATCGCCGGGCTCATCTGACGCATGATCAGGGCCTGGCCTGGCGCGTGCCCTCAA
GGCTGGACGCAGACAAGATGCACGAGGCCGCGCAGTCTTTCGTGGGCACGCATGATTTCACGACCTTCCGGGACAGCGAG
TGCCAGGCCCTCAGCCCGGTCAAGACGCTCACGCGGTTGGATGTAGCCCGTTATAACGACCGGATCGAGTTCACCTGCTC
GGCGCCGAGTTTCATTCACCGTCAGGTCCGCTCCCTGGTCGGCAGCCTGGTGGAGGTCGGCCGTGGGCGCCATCCGGTGA
AATGGGCGCGGGAAATTCTTGAGGCGGCAGACCGCACCCGCTGCGGGCCTGTGGCGCCGTCCGACGGATTGTTCCTGGAA
CGGGTAGATTACCCCGCGGGCTGA

Upstream 100 bases:

>100_bases
GGAAGGGCGGCCCGGAAACCGGCACACTCACCTATCCCAAAGCATTCGGCACCTGAAAGCCCGGTTGACCCGGCGCCCCG
GCTTTGGCAGCGGACATCGC

Downstream 100 bases:

>100_bases
CGCGACGACAAACTGTGCAGGCCCGGATTTCGCGGTTAGCCTCTCATCATAAAGAATGAGGAGGAGCGCCCGATGGCCAT
TGATTTCACGATTCCGGAAG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MPRYRLLIEYNGGPYQGWMRLPGLQTVQGALEAAAAQLDGGPVEVTGAGRTDAGVHATGQVAHMDLRVDRPNKVADAMNY
HLRPHPIAVLKADRVEEDFHARFSAIARHYRYIVINRRAHLTHDQGLAWRVPSRLDADKMHEAAQSFVGTHDFTTFRDSE
CQALSPVKTLTRLDVARYNDRIEFTCSAPSFIHRQVRSLVGSLVEVGRGRHPVKWAREILEAADRTRCGPVAPSDGLFLE
RVDYPAG

Sequences:

>Translated_247_residues
MPRYRLLIEYNGGPYQGWMRLPGLQTVQGALEAAAAQLDGGPVEVTGAGRTDAGVHATGQVAHMDLRVDRPNKVADAMNY
HLRPHPIAVLKADRVEEDFHARFSAIARHYRYIVINRRAHLTHDQGLAWRVPSRLDADKMHEAAQSFVGTHDFTTFRDSE
CQALSPVKTLTRLDVARYNDRIEFTCSAPSFIHRQVRSLVGSLVEVGRGRHPVKWAREILEAADRTRCGPVAPSDGLFLE
RVDYPAG
>Mature_246_residues
PRYRLLIEYNGGPYQGWMRLPGLQTVQGALEAAAAQLDGGPVEVTGAGRTDAGVHATGQVAHMDLRVDRPNKVADAMNYH
LRPHPIAVLKADRVEEDFHARFSAIARHYRYIVINRRAHLTHDQGLAWRVPSRLDADKMHEAAQSFVGTHDFTTFRDSEC
QALSPVKTLTRLDVARYNDRIEFTCSAPSFIHRQVRSLVGSLVEVGRGRHPVKWAREILEAADRTRCGPVAPSDGLFLER
VDYPAG

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=38.1818181818182, Blast_Score=134, Evalue=7e-32,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=42.1052631578947, Blast_Score=182, Evalue=2e-47,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=27.0627062706271, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_HYPNA (Q0C4U8)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759245.1
- ProteinModelPortal:   Q0C4U8
- SMR:   Q0C4U8
- STRING:   Q0C4U8
- GeneID:   4289509
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0515
- NMPDR:   fig|228405.5.peg.507
- TIGR:   HNE_0515
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- PhylomeDB:   Q0C4U8
- BioCyc:   HNEP81032:HNE_0515-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27598; Mature: 27466

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRYRLLIEYNGGPYQGWMRLPGLQTVQGALEAAAAQLDGGPVEVTGAGRTDAGVHATGQ
CCCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
VAHMDLRVDRPNKVADAMNYHLRPHPIAVLKADRVEEDFHARFSAIARHYRYIVINRRAH
EEEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCEE
LTHDQGLAWRVPSRLDADKMHEAAQSFVGTHDFTTFRDSECQALSPVKTLTRLDVARYND
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
RIEFTCSAPSFIHRQVRSLVGSLVEVGRGRHPVKWAREILEAADRTRCGPVAPSDGLFLE
CEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
RVDYPAG
ECCCCCC
>Mature Secondary Structure 
PRYRLLIEYNGGPYQGWMRLPGLQTVQGALEAAAAQLDGGPVEVTGAGRTDAGVHATGQ
CCEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
VAHMDLRVDRPNKVADAMNYHLRPHPIAVLKADRVEEDFHARFSAIARHYRYIVINRRAH
EEEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHEEEEEEECCEE
LTHDQGLAWRVPSRLDADKMHEAAQSFVGTHDFTTFRDSECQALSPVKTLTRLDVARYND
CCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
RIEFTCSAPSFIHRQVRSLVGSLVEVGRGRHPVKWAREILEAADRTRCGPVAPSDGLFLE
CEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEE
RVDYPAG
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA