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The map label for this gene is dacB [H]

Identifier: 114798405

GI number: 114798405

Start: 400364

End: 401806

Strand: Direct

Name: dacB [H]

Synonym: HNE_0402

Alternate gene names: 114798405

Gene position: 400364-401806 (Clockwise)

Preceding gene: 114797192

Following gene: 114800039

Centisome position: 10.81

GC content: 66.67

Gene sequence:

>1443_bases
ATGATCAGGCCCCGCTTTCTCTCTGGCGCGCTCGCCGCTGTCGTCTTTGCGGTGTCGGCCTGTACCACCACACCTGCCGA
GCCGCCCCCGCCAGCGCCCTTCGAGGCGATGCAGGTGGAGGGCGTGCGCTGGGGCGTTTCGGTCATGACGCTGGACGGGC
GGGAGGTGTTTTCGCGCAGCGCTGAGGAGCGGTTCATTCCGGCCTCCAACACCAAGGTGTTCACATCGGCCGCCGCGTTT
CACTATCTCACTGGCCTGGATACGCCAGACCCGGCCGGGGGCACGTCGCTCTGGCTGGAGCCGGGCGGGGAGGGCGAGGC
GCCTGATCTTGTGCTGATGGGCGCTGGCGATCCGGGCCTCATGGATAGTGCCGATTGCGTGGATAATTGCCTGCATCAGC
TCGCGGACGCTGTGGTCGCTGCCGGCATCACGCGCGTGGGAGATGTGATCGGGGACGACACCCTGCTGCCCGCCGCGCTC
TGGGGGCAGGGGTGGAGCTGGAACAATTTCGTCTGGTATTATACTGCGCCCATCTCAGCTTTGACGGTGAATGAGAATAC
GCTCGGATTGACCGTCTCTCCGGGCGCAGCGCCGGGCGAGCCAGCGGTGCTCAGCTGGGCGCCGGGAGATGATCTCCTCC
TGGTCGATAATAAAGTTATCACGGGACCGCCGGGCAGCTTGCAGACGCTGCGCATGGTGCGGGTGCCCGGCCTCGAGATG
CTTCGGCTCACCGAATATATTCCGGCTGATGCCGCGCCGCGCATGTATGCGGTGGCGATGCACGATCCGGCGGAGACAGC
GGCGCTGCGCTTTGTCCGTCTCCTGATAGAGCGCGGGGTAACCGTTGAGGGCCTGATGCGCGCGCGCCATGAGGCGGATG
ATAGTCCGGCCGAGCAGGTGCTGATCGCTCGTCTCACGCCGGCGCCGATCAAGGCAAGCATCGGCCGCATCCTCGTCAAC
AGCCAGAACCTGCATGCCGAGCTTTTGTTGCGCAAGATTGCGCTCGCGCGGGGGCAGCTTTCGCCTGAGGGCGGCCAGGA
AGTGCTCACCGCGCTGGTGGCTGAAGCGGGGCTGACGCCGGTGGAAGCGGAGCTCTTCGATGGCTCGGGCCTCTCCACCT
ATAACCGGGTCACGCCGCGCGGTATGGCGCAGTTCCTGCGCTGGGCTGCCGGCCAGCCTTGGGGAGACGCCTGGCGCGCC
ATGCTGCCCGTGGGCGGGGCAGACGGCACGCTGGCGCGCCGCTTCAAGGGCACGTCTCTGGAGGGCAAGGTGTTCGCCAA
GACCGGTTCGCTGCACGGCGTCAACGCGCTCTCCGGCTACATGACCGCGCGCAGCGGCCAGACGCTGGTGTTTGCCGTCT
ACGCCAATGACCGCCCGGCAGACGCCCCCTCGATCATCGCCGAGATGGACGCCAATCTGGTGCGCATCGCCGAAGCGAAT
TAG

Upstream 100 bases:

>100_bases
GCGCTATCCAAAGCATGACTGGCCGGACGACCCGGCAAATGCCCGCGCGCATGAGGGGCGCACCAAGAAACGCCTCTAGG
CAGGAAAAGGACGGTGACGG

Downstream 100 bases:

>100_bases
TCCGTAGGGCGATCAGTTCGGGCGGCGGGTCGTCTCGTCGTTCCAGTCCATGCCCATCGATTTCTTCATCCAGTCGGAGA
AGGTGCCCGGCTTTTTCAGG

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein; PBP [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MIRPRFLSGALAAVVFAVSACTTTPAEPPPPAPFEAMQVEGVRWGVSVMTLDGREVFSRSAEERFIPASNTKVFTSAAAF
HYLTGLDTPDPAGGTSLWLEPGGEGEAPDLVLMGAGDPGLMDSADCVDNCLHQLADAVVAAGITRVGDVIGDDTLLPAAL
WGQGWSWNNFVWYYTAPISALTVNENTLGLTVSPGAAPGEPAVLSWAPGDDLLLVDNKVITGPPGSLQTLRMVRVPGLEM
LRLTEYIPADAAPRMYAVAMHDPAETAALRFVRLLIERGVTVEGLMRARHEADDSPAEQVLIARLTPAPIKASIGRILVN
SQNLHAELLLRKIALARGQLSPEGGQEVLTALVAEAGLTPVEAELFDGSGLSTYNRVTPRGMAQFLRWAAGQPWGDAWRA
MLPVGGADGTLARRFKGTSLEGKVFAKTGSLHGVNALSGYMTARSGQTLVFAVYANDRPADAPSIIAEMDANLVRIAEAN

Sequences:

>Translated_480_residues
MIRPRFLSGALAAVVFAVSACTTTPAEPPPPAPFEAMQVEGVRWGVSVMTLDGREVFSRSAEERFIPASNTKVFTSAAAF
HYLTGLDTPDPAGGTSLWLEPGGEGEAPDLVLMGAGDPGLMDSADCVDNCLHQLADAVVAAGITRVGDVIGDDTLLPAAL
WGQGWSWNNFVWYYTAPISALTVNENTLGLTVSPGAAPGEPAVLSWAPGDDLLLVDNKVITGPPGSLQTLRMVRVPGLEM
LRLTEYIPADAAPRMYAVAMHDPAETAALRFVRLLIERGVTVEGLMRARHEADDSPAEQVLIARLTPAPIKASIGRILVN
SQNLHAELLLRKIALARGQLSPEGGQEVLTALVAEAGLTPVEAELFDGSGLSTYNRVTPRGMAQFLRWAAGQPWGDAWRA
MLPVGGADGTLARRFKGTSLEGKVFAKTGSLHGVNALSGYMTARSGQTLVFAVYANDRPADAPSIIAEMDANLVRIAEAN
>Mature_480_residues
MIRPRFLSGALAAVVFAVSACTTTPAEPPPPAPFEAMQVEGVRWGVSVMTLDGREVFSRSAEERFIPASNTKVFTSAAAF
HYLTGLDTPDPAGGTSLWLEPGGEGEAPDLVLMGAGDPGLMDSADCVDNCLHQLADAVVAAGITRVGDVIGDDTLLPAAL
WGQGWSWNNFVWYYTAPISALTVNENTLGLTVSPGAAPGEPAVLSWAPGDDLLLVDNKVITGPPGSLQTLRMVRVPGLEM
LRLTEYIPADAAPRMYAVAMHDPAETAALRFVRLLIERGVTVEGLMRARHEADDSPAEQVLIARLTPAPIKASIGRILVN
SQNLHAELLLRKIALARGQLSPEGGQEVLTALVAEAGLTPVEAELFDGSGLSTYNRVTPRGMAQFLRWAAGQPWGDAWRA
MLPVGGADGTLARRFKGTSLEGKVFAKTGSLHGVNALSGYMTARSGQTLVFAVYANDRPADAPSIIAEMDANLVRIAEAN

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=467, Percent_Identity=28.9079229122056, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 50753; Mature: 50753

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPRFLSGALAAVVFAVSACTTTPAEPPPPAPFEAMQVEGVRWGVSVMTLDGREVFSRS
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEEECEEEEEECCHHHHHCC
AEERFIPASNTKVFTSAAAFHYLTGLDTPDPAGGTSLWLEPGGEGEAPDLVLMGAGDPGL
HHHCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCC
MDSADCVDNCLHQLADAVVAAGITRVGDVIGDDTLLPAALWGQGWSWNNFVWYYTAPISA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEECCEEE
LTVNENTLGLTVSPGAAPGEPAVLSWAPGDDLLLVDNKVITGPPGSLQTLRMVRVPGLEM
EEECCCEEEEEECCCCCCCCCCEEEECCCCCEEEECCEEEECCCCCHHEEEEEECCCHHH
LRLTEYIPADAAPRMYAVAMHDPAETAALRFVRLLIERGVTVEGLMRARHEADDSPAEQV
HHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHE
LIARLTPAPIKASIGRILVNSQNLHAELLLRKIALARGQLSPEGGQEVLTALVAEAGLTP
EEEECCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
VEAELFDGSGLSTYNRVTPRGMAQFLRWAAGQPWGDAWRAMLPVGGADGTLARRFKGTSL
CCCEEECCCCCCHHCCCCHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHCCCCCC
EGKVFAKTGSLHGVNALSGYMTARSGQTLVFAVYANDRPADAPSIIAEMDANLVRIAEAN
CCEEEEECCCCCCCHHHHHHHEECCCCEEEEEEEECCCCCCCHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MIRPRFLSGALAAVVFAVSACTTTPAEPPPPAPFEAMQVEGVRWGVSVMTLDGREVFSRS
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCEEECEEEEEECCHHHHHCC
AEERFIPASNTKVFTSAAAFHYLTGLDTPDPAGGTSLWLEPGGEGEAPDLVLMGAGDPGL
HHHCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCC
MDSADCVDNCLHQLADAVVAAGITRVGDVIGDDTLLPAALWGQGWSWNNFVWYYTAPISA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEECCEEE
LTVNENTLGLTVSPGAAPGEPAVLSWAPGDDLLLVDNKVITGPPGSLQTLRMVRVPGLEM
EEECCCEEEEEECCCCCCCCCCEEEECCCCCEEEECCEEEECCCCCHHEEEEEECCCHHH
LRLTEYIPADAAPRMYAVAMHDPAETAALRFVRLLIERGVTVEGLMRARHEADDSPAEQV
HHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHE
LIARLTPAPIKASIGRILVNSQNLHAELLLRKIALARGQLSPEGGQEVLTALVAEAGLTP
EEEECCCCCHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
VEAELFDGSGLSTYNRVTPRGMAQFLRWAAGQPWGDAWRAMLPVGGADGTLARRFKGTSL
CCCEEECCCCCCHHCCCCHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCHHHHHCCCCCC
EGKVFAKTGSLHGVNALSGYMTARSGQTLVFAVYANDRPADAPSIIAEMDANLVRIAEAN
CCEEEEECCCCCCCHHHHHHHEECCCCEEEEEEEECCCCCCCHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1554361; 7305936 [H]