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The map label for this gene is purA

Identifier: 114798213

GI number: 114798213

Start: 3303596

End: 3304888

Strand: Reverse

Name: purA

Synonym: HNE_3124

Alternate gene names: 114798213

Gene position: 3304888-3303596 (Counterclockwise)

Preceding gene: 114800229

Following gene: 114799699

Centisome position: 89.2

GC content: 65.12

Gene sequence:

>1293_bases
ATGGCAGGTGTAGTGGTCGTCGGGGCTCAATGGGGCGACGAGGGCAAGGGCAAGATCGTTGACTGGCTCTCCAGCCGGGC
CGATGTCGTCGTGCGCTTCCAGGGCGGGCACAATGCCGGCCACACCCTCGTGATCGACGGGAAAGTATTCAAGCTGGCGC
TGCTGCCTTCAGGGCTCGTGCGCGGCGGCAAGCTCTCGGTCATCGGCAATGGCGTTGTGGTCGACCCGTGGCACATGCTG
ACCGAGATCGAAGGCATTCAGGCGCAAGGCATTGAAGTTTCTCCGGAAAGTCTGGTGCTGGCCGACAATGCCTCGCTGAT
CCTGCCCTGGCACAAGGACATCGACGCGGCCCGCGAAGGCGCACTCGGCGCCGCCCAGATCGGCACCACCAAGCGTGGCA
TTGGCCCGGCTTATGAAGACCGCGTGGGCCGCCGCGCCATCCGCGTCGCTGACCTTGCAGACCCCGCCGCCCTCGACCTC
AAGATCGAACGCTTGCTGGCCCATCACCGCCCGCTGCGCGCTGGCCTGGACTTGCCCGAACCCGATGGCGCCGCCCTGAA
AGCCGCGCTTCTGGAGATCGCGCCGCAGGTTCTGGCCTATGCCCAGCCTGTCTGGAAACTGCTGGACGAGAAGGTCCGCC
GGGGCAGCCGCATCCTGTTTGAGGGCGCCCAGGGCGTGATGCTGGACGTTGACCATGGCACCTACCCGTTTGTGACCTCT
TCGAGTGTTGTGGCAGGCAATGCCTCGGCCGGATCGGGCGTCGGGCCTGGGGCGATTTCCTATGTTCTGGGCCTTGCCAA
AGCCTATACGACGCGCGTCGGCGCCGGCCCGTTCCCGACCGAGCAGGACAATGACATCGGCAAGCGCCTCGGCACGGTGG
GCCATGAATTTGGCACCAATACCGGCCGCGCGCGCCGCTGCGGCTGGTTTGACTCGGTCATGGTGCGTCAGGCGTGCGCC
ACTTCGGGCGTCACGGGCCTCGCGCTGACCAAGCTCGATGTCCTTGATGGGTTTGAAGAAATCAAGATTTGCACGGCCTA
TAAGCTGAACGGCAAGACCATCGATTACTTCCCGGCCGGGCTCACCGATCAGGCCGCTGTCGAGCCGGTTTACGAAACGC
TCAAAGGCTGGAGCGGCTCAACCCGCGGGGCGCGCAGCTGGACGGATCTGCCGCCCGAAGCGGTCGTCTATGTGCGCCGC
CTGGAGGAACTTGTCGGCAAACCCTGCGCGCTGGTCTCGACCTCGCCGGAGCGGGAAGACGTGATCCTGATGCGCGACCC
GTTTGAGGGATAA

Upstream 100 bases:

>100_bases
CTTCGGCGAACAATGACTGTGTGAGGGGTGGCAAAAGGCCCCTTGCAATTGACCGCCGAATGCTGCCCTTGTCGCCGCGG
CTGAACAACGGAGCAGGTTC

Downstream 100 bases:

>100_bases
GCGGCGCTTATTTGTGTGGATAAGGGGCCAAAAAACTCCAAAAAAACTCCAACGGTCACCCCGAAAACTCCAAGAAAACT
CCAACGCCGTTGGAGTTTTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAGVVVVGAQWGDEGKGKIVDWLSSRADVVVRFQGGHNAGHTLVIDGKVFKLALLPSGLVRGGKLSVIGNGVVVDPWHML
TEIEGIQAQGIEVSPESLVLADNASLILPWHKDIDAAREGALGAAQIGTTKRGIGPAYEDRVGRRAIRVADLADPAALDL
KIERLLAHHRPLRAGLDLPEPDGAALKAALLEIAPQVLAYAQPVWKLLDEKVRRGSRILFEGAQGVMLDVDHGTYPFVTS
SSVVAGNASAGSGVGPGAISYVLGLAKAYTTRVGAGPFPTEQDNDIGKRLGTVGHEFGTNTGRARRCGWFDSVMVRQACA
TSGVTGLALTKLDVLDGFEEIKICTAYKLNGKTIDYFPAGLTDQAAVEPVYETLKGWSGSTRGARSWTDLPPEAVVYVRR
LEELVGKPCALVSTSPEREDVILMRDPFEG

Sequences:

>Translated_430_residues
MAGVVVVGAQWGDEGKGKIVDWLSSRADVVVRFQGGHNAGHTLVIDGKVFKLALLPSGLVRGGKLSVIGNGVVVDPWHML
TEIEGIQAQGIEVSPESLVLADNASLILPWHKDIDAAREGALGAAQIGTTKRGIGPAYEDRVGRRAIRVADLADPAALDL
KIERLLAHHRPLRAGLDLPEPDGAALKAALLEIAPQVLAYAQPVWKLLDEKVRRGSRILFEGAQGVMLDVDHGTYPFVTS
SSVVAGNASAGSGVGPGAISYVLGLAKAYTTRVGAGPFPTEQDNDIGKRLGTVGHEFGTNTGRARRCGWFDSVMVRQACA
TSGVTGLALTKLDVLDGFEEIKICTAYKLNGKTIDYFPAGLTDQAAVEPVYETLKGWSGSTRGARSWTDLPPEAVVYVRR
LEELVGKPCALVSTSPEREDVILMRDPFEG
>Mature_429_residues
AGVVVVGAQWGDEGKGKIVDWLSSRADVVVRFQGGHNAGHTLVIDGKVFKLALLPSGLVRGGKLSVIGNGVVVDPWHMLT
EIEGIQAQGIEVSPESLVLADNASLILPWHKDIDAAREGALGAAQIGTTKRGIGPAYEDRVGRRAIRVADLADPAALDLK
IERLLAHHRPLRAGLDLPEPDGAALKAALLEIAPQVLAYAQPVWKLLDEKVRRGSRILFEGAQGVMLDVDHGTYPFVTSS
SVVAGNASAGSGVGPGAISYVLGLAKAYTTRVGAGPFPTEQDNDIGKRLGTVGHEFGTNTGRARRCGWFDSVMVRQACAT
SGVTGLALTKLDVLDGFEEIKICTAYKLNGKTIDYFPAGLTDQAAVEPVYETLKGWSGSTRGARSWTDLPPEAVVYVRRL
EELVGKPCALVSTSPEREDVILMRDPFEG

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.131455399061, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI34577063, Length=427, Percent_Identity=41.4519906323185, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=42.4242424242424, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=48.4848484848485, Blast_Score=411, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=40.3263403263403, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=40.3263403263403, Blast_Score=312, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=39.9532710280374, Blast_Score=313, Evalue=3e-86,
Organism=Drosophila melanogaster, GI21356033, Length=420, Percent_Identity=43.3333333333333, Blast_Score=333, Evalue=2e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_HYPNA (Q0BXJ3)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761800.1
- ProteinModelPortal:   Q0BXJ3
- SMR:   Q0BXJ3
- STRING:   Q0BXJ3
- GeneID:   4287837
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3124
- NMPDR:   fig|228405.5.peg.2992
- TIGR:   HNE_3124
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   ERDDTIV
- PhylomeDB:   Q0BXJ3
- ProtClustDB:   CLSK2606020
- BioCyc:   HNEP81032:HNE_3124-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 45683; Mature: 45552

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 224-224 BINDING 239-239 BINDING 303-303 BINDING 305-305

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVVVVGAQWGDEGKGKIVDWLSSRADVVVRFQGGHNAGHTLVIDGKVFKLALLPSGLV
CCCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEEECCHHHC
RGGKLSVIGNGVVVDPWHMLTEIEGIQAQGIEVSPESLVLADNASLILPWHKDIDAAREG
CCCEEEEEECCEEECCHHHHHHHCCCCCCCEEECCCCEEEECCCCEEECCCCCHHHHHCC
ALGAAQIGTTKRGIGPAYEDRVGRRAIRVADLADPAALDLKIERLLAHHRPLRAGLDLPE
CCCHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PDGAALKAALLEIAPQVLAYAQPVWKLLDEKVRRGSRILFEGAQGVMLDVDHGTYPFVTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCEEEC
SSVVAGNASAGSGVGPGAISYVLGLAKAYTTRVGAGPFPTEQDNDIGKRLGTVGHEFGTN
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
TGRARRCGWFDSVMVRQACATSGVTGLALTKLDVLDGFEEIKICTAYKLNGKTIDYFPAG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHEEHHHHHCCHHHEEEEEEEEECCCEEEECCCC
LTDQAAVEPVYETLKGWSGSTRGARSWTDLPPEAVVYVRRLEELVGKPCALVSTSPERED
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCC
VILMRDPFEG
EEEECCCCCC
>Mature Secondary Structure 
AGVVVVGAQWGDEGKGKIVDWLSSRADVVVRFQGGHNAGHTLVIDGKVFKLALLPSGLV
CCEEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCEEEECCCEEEEEECCHHHC
RGGKLSVIGNGVVVDPWHMLTEIEGIQAQGIEVSPESLVLADNASLILPWHKDIDAAREG
CCCEEEEEECCEEECCHHHHHHHCCCCCCCEEECCCCEEEECCCCEEECCCCCHHHHHCC
ALGAAQIGTTKRGIGPAYEDRVGRRAIRVADLADPAALDLKIERLLAHHRPLRAGLDLPE
CCCHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
PDGAALKAALLEIAPQVLAYAQPVWKLLDEKVRRGSRILFEGAQGVMLDVDHGTYPFVTS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCEEEC
SSVVAGNASAGSGVGPGAISYVLGLAKAYTTRVGAGPFPTEQDNDIGKRLGTVGHEFGTN
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCC
TGRARRCGWFDSVMVRQACATSGVTGLALTKLDVLDGFEEIKICTAYKLNGKTIDYFPAG
CCCCCCCCHHHHHHHHHHHHHCCCCHHHEEHHHHHCCHHHEEEEEEEEECCCEEEECCCC
LTDQAAVEPVYETLKGWSGSTRGARSWTDLPPEAVVYVRRLEELVGKPCALVSTSPERED
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCC
VILMRDPFEG
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA