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The map label for this gene is cysH [H]

Identifier: 114798004

GI number: 114798004

Start: 322398

End: 323132

Strand: Direct

Name: cysH [H]

Synonym: HNE_0327

Alternate gene names: 114798004

Gene position: 322398-323132 (Clockwise)

Preceding gene: 114799901

Following gene: 114798280

Centisome position: 8.7

GC content: 63.54

Gene sequence:

>735_bases
ATGGCCTTTGAAACCGACACATTCGAGGAGCCCAAAGACCGGCTCGCGCGCCTTAACGGAGAACTCCGGAATGCGTCCGC
GCAGGAAATCCTCCGCGCCGCCATTTCCCGCGAATGGCCCGGCGGGCTCACCTATGTGTCCAGTTTCGGCGCCGAAAGCG
TCGTCATGCTCGCCCTCATCGCTGAGGTCGATCCCTCCCTGCCAATCGTCTTCATCGACACCGGCATGCATTTCCCTCAG
ACGCTGGACTATAAAGACGAGGTCATCGAACGCCTCGGCCTCACCGGCGTGCGCAATATCACGCCCAGCGAGACTGAGCG
CAAAGTGCTCGACCCCAAGAACATGCTCTGGAAAACCGATGCCGACGCCTGCTGCGCCCTGCGCAAGGTGCGCCCGCTGG
AGCCGGCGCTGGAGGGCTTCGGCGCCTGGGTCACGGGCCGTAAACGCTTCCATGGCGGCGTCCGGATGAGCCTGCCGGTG
TTTGAATTTGCCGATGGCCGCTACAAGGTGAACCCGCTCGCCAACTGGACGCAAGGCGACGTGGACGCCTATCTCGAAGA
GAAAAACCTGCCCCGCCACCCGCTGGTCTCGCAAGGCTATCCTTCGATCGGCTGCTGGCCCTGCACCCAGCCTGCCGCAG
ACCCCAACGACCCCCGTTCGGGCCGCTGGGTCGGCCAGGAAAAGTCCGAATGCGGACTGCACGTCGAACGCAATGAACGG
CCACGGGTTTTCTGA

Upstream 100 bases:

>100_bases
TACTGGAAGCATTGAGCGAATATAGCGTGGCCTATCAGCCCGCTGCCGATAGTTCTCTCCCCGCCTTCCGCAGACGTAGC
TGACCCCAGGAAGACGGCCC

Downstream 100 bases:

>100_bases
TTATTGCTCGCGGCTGTTGCTGAGCCCTTCGGGCTCAGCGCCGCTGCGCCTTGCTTGTGGGGCGCTCCGCGTTTGAGCTC
ATCCTGAAGGATAGAGCAGG

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MAFETDTFEEPKDRLARLNGELRNASAQEILRAAISREWPGGLTYVSSFGAESVVMLALIAEVDPSLPIVFIDTGMHFPQ
TLDYKDEVIERLGLTGVRNITPSETERKVLDPKNMLWKTDADACCALRKVRPLEPALEGFGAWVTGRKRFHGGVRMSLPV
FEFADGRYKVNPLANWTQGDVDAYLEEKNLPRHPLVSQGYPSIGCWPCTQPAADPNDPRSGRWVGQEKSECGLHVERNER
PRVF

Sequences:

>Translated_244_residues
MAFETDTFEEPKDRLARLNGELRNASAQEILRAAISREWPGGLTYVSSFGAESVVMLALIAEVDPSLPIVFIDTGMHFPQ
TLDYKDEVIERLGLTGVRNITPSETERKVLDPKNMLWKTDADACCALRKVRPLEPALEGFGAWVTGRKRFHGGVRMSLPV
FEFADGRYKVNPLANWTQGDVDAYLEEKNLPRHPLVSQGYPSIGCWPCTQPAADPNDPRSGRWVGQEKSECGLHVERNER
PRVF
>Mature_243_residues
AFETDTFEEPKDRLARLNGELRNASAQEILRAAISREWPGGLTYVSSFGAESVVMLALIAEVDPSLPIVFIDTGMHFPQT
LDYKDEVIERLGLTGVRNITPSETERKVLDPKNMLWKTDADACCALRKVRPLEPALEGFGAWVTGRKRFHGGVRMSLPVF
EFADGRYKVNPLANWTQGDVDAYLEEKNLPRHPLVSQGYPSIGCWPCTQPAADPNDPRSGRWVGQEKSECGLHVERNERP
RVF

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=226, Percent_Identity=31.858407079646, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325425, Length=205, Percent_Identity=33.1707317073171, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 27384; Mature: 27253

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFETDTFEEPKDRLARLNGELRNASAQEILRAAISREWPGGLTYVSSFGAESVVMLALI
CCCCCCCCCCHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
AEVDPSLPIVFIDTGMHFPQTLDYKDEVIERLGLTGVRNITPSETERKVLDPKNMLWKTD
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHEEECC
ADACCALRKVRPLEPALEGFGAWVTGRKRFHGGVRMSLPVFEFADGRYKVNPLANWTQGD
HHHHHHHHHCCCCCHHHHHHCHHHCCCHHHCCCEEEECCCEEECCCCEEECCCCCCCCCH
VDAYLEEKNLPRHPLVSQGYPSIGCWPCTQPAADPNDPRSGRWVGQEKSECGLHVERNER
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
PRVF
CCCC
>Mature Secondary Structure 
AFETDTFEEPKDRLARLNGELRNASAQEILRAAISREWPGGLTYVSSFGAESVVMLALI
CCCCCCCCCHHHHHHHHCCHHCCCCHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHH
AEVDPSLPIVFIDTGMHFPQTLDYKDEVIERLGLTGVRNITPSETERKVLDPKNMLWKTD
HHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHEEECC
ADACCALRKVRPLEPALEGFGAWVTGRKRFHGGVRMSLPVFEFADGRYKVNPLANWTQGD
HHHHHHHHHCCCCCHHHHHHCHHHCCCHHHCCCEEEECCCEEECCCCEEECCCCCCCCCH
VDAYLEEKNLPRHPLVSQGYPSIGCWPCTQPAADPNDPRSGRWVGQEKSECGLHVERNER
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCC
PRVF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA