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The map label for this gene is dnaB [H]

Identifier: 114797768

GI number: 114797768

Start: 2249981

End: 2251471

Strand: Direct

Name: dnaB [H]

Synonym: HNE_2164

Alternate gene names: 114797768

Gene position: 2249981-2251471 (Clockwise)

Preceding gene: 114798663

Following gene: 114797205

Centisome position: 60.73

GC content: 61.03

Gene sequence:

>1491_bases
ATGTCTGAGCAAAAGCCGGGAAGCGGTTCCATCTCGCCGCCGAACAACCTTGCCGCCGAAGCCGCCGTGCTGGGCGCAAT
CTTGTTCGACAATAACGTCTACCAGTTCATTGCGGACATCCTCACGCCGCCTGACTTCTACGCGCCCGCGCACCGCGAAG
TGTATGCGGTTGCGTCCAACATGATTCAGACCGGACGGGTCGCAGACGGCGTGACGCTGCGCGAATATTTCGAGCAGAAG
GAAAAGATCGCCGAAATCGGCGGCGCCAAATATCTGGTGGACCTGCTCGACGCCGCAGCCTTCGGGCCGGAGGTGAAGGA
TTATGCGCGGATCATCCGCGACCTTGCCATCCGGCGCGAGCTGATCACGGTCGGCGGCACCATTCAGTCCCAGGCGATCA
TGCCGGAACAGGACGACACCGGCTCAAGTCTCATCAACAAGGCCGAGCGCGCACTGTTTGATCTGGCTGAACGCGGCAGC
GCAGAACGGGGCTTTTCTAGCTTTGCCGAGGCGCTCGCCGAATCGCTGATCACGGCTGAAGCCGCGTTCAAGCGCGGCGG
CAAAATTGCCGGCATCCCGACCGGCCTGCGCGATCTCGACAACCAGCTCGGCGGCTTTCACCGGTCCGACCTTCTCATCC
TCGCCGGCCGCCCCTCGATGGGGAAAACGTCGCTGGCGACCAACATCGCCTACAATGCCGCCGCCGCGTACAAATACGAA
ACTCAGGAAGACGGCTCCAAAAAGACGATGGACGGCGCGGTCGTCGCCTTCTTCTCGCTCGAGATGTCGAACGAGCAGCT
TGCCACGCGTATTCTGGCCGAACGTACCGGCATCAACGCCCACCGCATCCGCCAGGGCGATCTGACCAAGGCGGACTTTG
AACAGCTCGTCGAAGCGACCTCAGAGCTGCAGAACCTGCCGCTGTATATCGACGATACCGGCGGTATTTCTATCAGTGAG
CTGACAGCCCGCGCGCGGCGCCTTCAGCGCTCGGTTGGCCTCGACATGATCGTGATCGACTATCTCCAGCTCATCACCGT
TTCGAGTTCCGGCGGGCAGGCAAACCGCGTTCAGGAGGTGACCCAGATCACCACTGCGCTCAAAGCATTGGCGAAGGATC
TCAACGTCCCCATCGTCGCCCTGTCGCAGCTCTCGCGCGCCGTTGAGCAGCGCGACGACAAGCGCCCGCAACTCTCAGAC
CTTCGCGAATCGGGCTCGATCGAGCAGGACGCCGACATCGTGATGTTCGTGTTCCGGGAGGAATACTACCTCGCTCGGAC
AGAGCCCGCCGCCGATCCGAATGATCCGACATCAAACGACAAATGGAAAGCCTGGCGCTCGCGTATGGACCAGGTTTATG
GAACGGCGGAAGTGATTGTGTCCAAGCAGCGTCACGGGCCGATCGGCACCGTGAAGCTCGCCTTCGATGCGAACGTCACA
CGGTTCGGTGACCTCGATAAATCGCGGATGACGGAAGATATTCTGGAGTAG

Upstream 100 bases:

>100_bases
CTGCGCGCGGCGACTCACAGAAATTGCCCTGATCCTGACTCCATGAGTCGCAAATCAAACTCCGCTTGCTATAGTAAACG
TTTGCGCCACGGGGGTGCCA

Downstream 100 bases:

>100_bases
GCTTAGTCCAGCCCTGCCCTCAGCAGGTCCAGCGCCTCTGCTGCAAACAGGCGCATGTTCGCCTCCCGGTCAGAAATGCC
GGTCTCCAGCGTGCGCGAGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSEQKPGSGSISPPNNLAAEAAVLGAILFDNNVYQFIADILTPPDFYAPAHREVYAVASNMIQTGRVADGVTLREYFEQK
EKIAEIGGAKYLVDLLDAAAFGPEVKDYARIIRDLAIRRELITVGGTIQSQAIMPEQDDTGSSLINKAERALFDLAERGS
AERGFSSFAEALAESLITAEAAFKRGGKIAGIPTGLRDLDNQLGGFHRSDLLILAGRPSMGKTSLATNIAYNAAAAYKYE
TQEDGSKKTMDGAVVAFFSLEMSNEQLATRILAERTGINAHRIRQGDLTKADFEQLVEATSELQNLPLYIDDTGGISISE
LTARARRLQRSVGLDMIVIDYLQLITVSSSGGQANRVQEVTQITTALKALAKDLNVPIVALSQLSRAVEQRDDKRPQLSD
LRESGSIEQDADIVMFVFREEYYLARTEPAADPNDPTSNDKWKAWRSRMDQVYGTAEVIVSKQRHGPIGTVKLAFDANVT
RFGDLDKSRMTEDILE

Sequences:

>Translated_496_residues
MSEQKPGSGSISPPNNLAAEAAVLGAILFDNNVYQFIADILTPPDFYAPAHREVYAVASNMIQTGRVADGVTLREYFEQK
EKIAEIGGAKYLVDLLDAAAFGPEVKDYARIIRDLAIRRELITVGGTIQSQAIMPEQDDTGSSLINKAERALFDLAERGS
AERGFSSFAEALAESLITAEAAFKRGGKIAGIPTGLRDLDNQLGGFHRSDLLILAGRPSMGKTSLATNIAYNAAAAYKYE
TQEDGSKKTMDGAVVAFFSLEMSNEQLATRILAERTGINAHRIRQGDLTKADFEQLVEATSELQNLPLYIDDTGGISISE
LTARARRLQRSVGLDMIVIDYLQLITVSSSGGQANRVQEVTQITTALKALAKDLNVPIVALSQLSRAVEQRDDKRPQLSD
LRESGSIEQDADIVMFVFREEYYLARTEPAADPNDPTSNDKWKAWRSRMDQVYGTAEVIVSKQRHGPIGTVKLAFDANVT
RFGDLDKSRMTEDILE
>Mature_495_residues
SEQKPGSGSISPPNNLAAEAAVLGAILFDNNVYQFIADILTPPDFYAPAHREVYAVASNMIQTGRVADGVTLREYFEQKE
KIAEIGGAKYLVDLLDAAAFGPEVKDYARIIRDLAIRRELITVGGTIQSQAIMPEQDDTGSSLINKAERALFDLAERGSA
ERGFSSFAEALAESLITAEAAFKRGGKIAGIPTGLRDLDNQLGGFHRSDLLILAGRPSMGKTSLATNIAYNAAAAYKYET
QEDGSKKTMDGAVVAFFSLEMSNEQLATRILAERTGINAHRIRQGDLTKADFEQLVEATSELQNLPLYIDDTGGISISEL
TARARRLQRSVGLDMIVIDYLQLITVSSSGGQANRVQEVTQITTALKALAKDLNVPIVALSQLSRAVEQRDDKRPQLSDL
RESGSIEQDADIVMFVFREEYYLARTEPAADPNDPTSNDKWKAWRSRMDQVYGTAEVIVSKQRHGPIGTVKLAFDANVTR
FGDLDKSRMTEDILE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=474, Percent_Identity=36.9198312236287, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54264; Mature: 54133

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQKPGSGSISPPNNLAAEAAVLGAILFDNNVYQFIADILTPPDFYAPAHREVYAVASN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MIQTGRVADGVTLREYFEQKEKIAEIGGAKYLVDLLDAAAFGPEVKDYARIIRDLAIRRE
HHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LITVGGTIQSQAIMPEQDDTGSSLINKAERALFDLAERGSAERGFSSFAEALAESLITAE
HHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AAFKRGGKIAGIPTGLRDLDNQLGGFHRSDLLILAGRPSMGKTSLATNIAYNAAAAYKYE
HHHHCCCCEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEEC
TQEDGSKKTMDGAVVAFFSLEMSNEQLATRILAERTGINAHRIRQGDLTKADFEQLVEAT
CCCCCCCHHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHH
SELQNLPLYIDDTGGISISELTARARRLQRSVGLDMIVIDYLQLITVSSSGGQANRVQEV
HHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCHHHHHHH
TQITTALKALAKDLNVPIVALSQLSRAVEQRDDKRPQLSDLRESGSIEQDADIVMFVFRE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEEEEC
EYYLARTEPAADPNDPTSNDKWKAWRSRMDQVYGTAEVIVSKQRHGPIGTVKLAFDANVT
CHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCCH
RFGDLDKSRMTEDILE
HCCCCCHHHHHHHHHC
>Mature Secondary Structure 
SEQKPGSGSISPPNNLAAEAAVLGAILFDNNVYQFIADILTPPDFYAPAHREVYAVASN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHH
MIQTGRVADGVTLREYFEQKEKIAEIGGAKYLVDLLDAAAFGPEVKDYARIIRDLAIRRE
HHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LITVGGTIQSQAIMPEQDDTGSSLINKAERALFDLAERGSAERGFSSFAEALAESLITAE
HHHHCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AAFKRGGKIAGIPTGLRDLDNQLGGFHRSDLLILAGRPSMGKTSLATNIAYNAAAAYKYE
HHHHCCCCEECCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHEEEEC
TQEDGSKKTMDGAVVAFFSLEMSNEQLATRILAERTGINAHRIRQGDLTKADFEQLVEAT
CCCCCCCHHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHH
SELQNLPLYIDDTGGISISELTARARRLQRSVGLDMIVIDYLQLITVSSSGGQANRVQEV
HHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCHHHHHHH
TQITTALKALAKDLNVPIVALSQLSRAVEQRDDKRPQLSDLRESGSIEQDADIVMFVFRE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEEEEC
EYYLARTEPAADPNDPTSNDKWKAWRSRMDQVYGTAEVIVSKQRHGPIGTVKLAFDANVT
CHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCCH
RFGDLDKSRMTEDILE
HCCCCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA