Click here to switch to the map view.

The map label for this gene is rlmH

Identifier: 114797356

GI number: 114797356

Start: 297992

End: 298456

Strand: Direct

Name: rlmH

Synonym: HNE_0304

Alternate gene names: 114797356

Gene position: 297992-298456 (Clockwise)

Preceding gene: 114798826

Following gene: 114798052

Centisome position: 8.04

GC content: 67.1

Gene sequence:

>465_bases
GTGCGCCTCCAGATCCTTGCTGTCGGCCGTCTCAAGGACGGGCCGGAACGCGACCTCACCGATGATTATATCCGCCGCGC
GTCCGCAATGGCGCGCGGTCTTGGTTTCAAGGGCCCTGAAGAAGCCGAGATTGCCAGTGGCGGCGGCATGGACGCCGAAG
GCGCCCGGCTTCTCGCCCGCATTCCTGAAGGCGCCCGCATCATCCGCCTGGATGAAGGCGGGGAAAACCTCACTTCGGAA
GCCTTTGCCTCCCGCCTCTCGCGCTGGCGCGATGATGGCGAGCGCGATACCTGCTTTCTGATCGGGGGCGCAGAAGGCTA
CGCGCCGGACGTGCGCGCCGCCGCGCCCCAGACGCTGGCCTTCGGTGTGCAGACATGGCCCCACCGGCTGGTGCGCGCCA
TGCTGGCCGAGCAGCTTTACCGGGCGATGACCATTCTGGCGGGCACGCCTTATCACAAGGCCTGA

Upstream 100 bases:

>100_bases
ATCGTCCATCTGTTCCGCCCGGAAGTGCGTGAATTCTACAATCTGGAAAAGATCTGGGTTTCCGACAAATCGGCCCACCG
CACCAGCGCGACCTGAGGCC

Downstream 100 bases:

>100_bases
AGCTGGCGGTCTTTCGCCTCGGGCGGTTGCACTCGCGCATTTGCCGGTTCTAATGGCGCCTGCTGAAAATGGGGCGGCAG
CATGAGCGGGACACTAAGAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MRLQILAVGRLKDGPERDLTDDYIRRASAMARGLGFKGPEEAEIASGGGMDAEGARLLARIPEGARIIRLDEGGENLTSE
AFASRLSRWRDDGERDTCFLIGGAEGYAPDVRAAAPQTLAFGVQTWPHRLVRAMLAEQLYRAMTILAGTPYHKA

Sequences:

>Translated_154_residues
MRLQILAVGRLKDGPERDLTDDYIRRASAMARGLGFKGPEEAEIASGGGMDAEGARLLARIPEGARIIRLDEGGENLTSE
AFASRLSRWRDDGERDTCFLIGGAEGYAPDVRAAAPQTLAFGVQTWPHRLVRAMLAEQLYRAMTILAGTPYHKA
>Mature_154_residues
MRLQILAVGRLKDGPERDLTDDYIRRASAMARGLGFKGPEEAEIASGGGMDAEGARLLARIPEGARIIRLDEGGENLTSE
AFASRLSRWRDDGERDTCFLIGGAEGYAPDVRAAAPQTLAFGVQTWPHRLVRAMLAEQLYRAMTILAGTPYHKA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=163, Percent_Identity=37.4233128834356, Blast_Score=90, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_HYPNA (Q0C5F9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759034.1
- ProteinModelPortal:   Q0C5F9
- SMR:   Q0C5F9
- STRING:   Q0C5F9
- GeneID:   4287210
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0304
- NMPDR:   fig|228405.5.peg.299
- TIGR:   HNE_0304
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- PhylomeDB:   Q0C5F9
- ProtClustDB:   CLSK2531652
- BioCyc:   HNEP81032:HNE_0304-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16785; Mature: 16785

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLQILAVGRLKDGPERDLTDDYIRRASAMARGLGFKGPEEAEIASGGGMDAEGARLLAR
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHC
IPEGARIIRLDEGGENLTSEAFASRLSRWRDDGERDTCFLIGGAEGYAPDVRAAAPQTLA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
FGVQTWPHRLVRAMLAEQLYRAMTILAGTPYHKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLQILAVGRLKDGPERDLTDDYIRRASAMARGLGFKGPEEAEIASGGGMDAEGARLLAR
CEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHC
IPEGARIIRLDEGGENLTSEAFASRLSRWRDDGERDTCFLIGGAEGYAPDVRAAAPQTLA
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHH
FGVQTWPHRLVRAMLAEQLYRAMTILAGTPYHKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA