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The map label for this gene is nfdA [H]

Identifier: 114797081

GI number: 114797081

Start: 3440070

End: 3441809

Strand: Reverse

Name: nfdA [H]

Synonym: HNE_3278

Alternate gene names: 114797081

Gene position: 3441809-3440070 (Counterclockwise)

Preceding gene: 114800031

Following gene: 114800111

Centisome position: 92.9

GC content: 64.54

Gene sequence:

>1740_bases
ATGCCATCGAGGGCACGGACCGGCTTATGGCGCAGCCTCGTGGTGGCAGGCGCTCTGCTCGCAGGGTGCGCCGCGCCCCA
GGCGCTCAGCGGTGAGGGCGCCGGCGAGGGTGCTACGGTATTTCTCAACGCCCGGATCATTACCGTGGATGAGACCCGGC
CCGAGGCAGAGGCGGTGGCGGTAAAGGACGGGCGCATCCTTGCTGTCGGAACGCGCGAAGAGGTGACGCGCATGGCGGGC
GCAGGCGCCGCGCTGCGCGATCTGGGCGGGGCAACCCTCGCGCCGGGTTTCATCGACACGCACGGCCACTTCGCCCTCAT
GGCGCAGGCCGCCGGCATGGCCAATATTCAGCCCCCGCCCGCCGGCCCGGTCCGCACGATCCCGGAATTGCAGGCCGAAC
TCGAAATCTGGAGCGGGCGACACCCGGACGCCGCCTGGATCACCGGCTGGGGGTATGACGATTCCCTGCTGGCCGAGCAG
CGCCACCCGACCCGGCAGGACCTTGACGCCGTGTCGACGGAAAAGCCGGTGATGCTGATGCACACCTCGGCCCATTTTGC
GGTGTGCAATACCCGCTGCCTTGAAATACTCGGGCTCACGGCGGAAACGCCCGATCCCTCCGGCGGGGTCATCCGCAGGG
AGCCCGACGGGCGCACGCCCAATGGTGTGCTTGAAGAATCCGCGCTCTATCTGCTTTACCAGCACCTGTCCCAGCCCGGC
GAAAGCCAGCGCATTGCGGGCATCCTGGAAACCCAGACCCTCTATGCCCGCTATGGCATCACCACCGTTCAGGAAGGCGC
AGCCCGGCCCCAGAATATCCAGGATCTGAAAAAGGTTGCCGGTCAGGGCGGGCTTTATCTGGATGTCGTTGCCTACCAGC
TGTTCCCGGACGGCGCGATGATTGCGGAGGATTTCACGGTTTCGAAAACCTATGAAAACCGCTTCCGGGTCGGGGGCATC
AAGCTGCTGCTCGATGGCTCGCTCCAGGGCAAGACAGGCTGGATTACCAAACCCTATGTCAGCCCGCCTGCGGGGCAGGC
GGAGGGCTATTCAGGCTATCGCATTCATACCGATGAAAGCGTCGCGGCCCTGTTTGAGGCTGCCTACAGCCGGGACATCC
AGATCATCGCGCATGCCAATGGCGACCGGGCAATTGATCAGTATCTGGACATGATCGCGCCGGTGATCGCGGCCCATCCG
GAGCAGGATGCCCGCCCGGTCATCATTCACGCCCAGGCAGCCCGGCAGGACCAGCTGGAAGAGATGGCGGCGGGCAACAT
CATTCCGTCCTTCTTTGCGGCGCATCCCTTCTATTGGGGCGATTGGCACCGGGACGCAGTGCTGGGCCCAGAGCGGGCGG
CCTTCATCAGCCCGCTCGCCGCCGCAAAAGCGCTCGGGATTCCGTATACGGTGCACAATGATCCCCCGGTCGTCCCGCCC
GATGTGATCCGGCTGCTCTGGGTGGCCGTGAACCGCGAGACCCGATCGGGGGAAATCCTTGGCCCGGACCAGCGCGCAGG
CCCGATGGACGCCCTGCGCGCGGTGACGCTGAATGCGGCCCGCCAGTATTTCGAGGAAGACGAGAAAGGCTCCATCACGC
CCGGCAAGAAGGCAGACTTCGTGGTCCTGTCAGATAATCCGCTGACGATAGACCCCGCTTCCATGCGCGATATTCGCATC
CTTGAAACCATCAAGGAAGGCGTCACCATCTACAGCGCTGAATTGCCCGAGGACAAATGA

Upstream 100 bases:

>100_bases
TCCAGGGTCTGCTACCGGGAAGGTAAGCGCAAGGCTTGCCATCCAGGGCCGGAATGGCGGATGGTTTCGCAAAACAAGAA
ACAATCAGGGGGAGATCACC

Downstream 100 bases:

>100_bases
CCATGCAAACCACGCTTGCACTGCTTCAGCCGCTTCTTCCCGAGCTCGCCGCCCGCGCGGGAGATATGGAGGCGGCGCGG
CGCCTTCCGGCAGACCTTGC

Product: amidohydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MPSRARTGLWRSLVVAGALLAGCAAPQALSGEGAGEGATVFLNARIITVDETRPEAEAVAVKDGRILAVGTREEVTRMAG
AGAALRDLGGATLAPGFIDTHGHFALMAQAAGMANIQPPPAGPVRTIPELQAELEIWSGRHPDAAWITGWGYDDSLLAEQ
RHPTRQDLDAVSTEKPVMLMHTSAHFAVCNTRCLEILGLTAETPDPSGGVIRREPDGRTPNGVLEESALYLLYQHLSQPG
ESQRIAGILETQTLYARYGITTVQEGAARPQNIQDLKKVAGQGGLYLDVVAYQLFPDGAMIAEDFTVSKTYENRFRVGGI
KLLLDGSLQGKTGWITKPYVSPPAGQAEGYSGYRIHTDESVAALFEAAYSRDIQIIAHANGDRAIDQYLDMIAPVIAAHP
EQDARPVIIHAQAARQDQLEEMAAGNIIPSFFAAHPFYWGDWHRDAVLGPERAAFISPLAAAKALGIPYTVHNDPPVVPP
DVIRLLWVAVNRETRSGEILGPDQRAGPMDALRAVTLNAARQYFEEDEKGSITPGKKADFVVLSDNPLTIDPASMRDIRI
LETIKEGVTIYSAELPEDK

Sequences:

>Translated_579_residues
MPSRARTGLWRSLVVAGALLAGCAAPQALSGEGAGEGATVFLNARIITVDETRPEAEAVAVKDGRILAVGTREEVTRMAG
AGAALRDLGGATLAPGFIDTHGHFALMAQAAGMANIQPPPAGPVRTIPELQAELEIWSGRHPDAAWITGWGYDDSLLAEQ
RHPTRQDLDAVSTEKPVMLMHTSAHFAVCNTRCLEILGLTAETPDPSGGVIRREPDGRTPNGVLEESALYLLYQHLSQPG
ESQRIAGILETQTLYARYGITTVQEGAARPQNIQDLKKVAGQGGLYLDVVAYQLFPDGAMIAEDFTVSKTYENRFRVGGI
KLLLDGSLQGKTGWITKPYVSPPAGQAEGYSGYRIHTDESVAALFEAAYSRDIQIIAHANGDRAIDQYLDMIAPVIAAHP
EQDARPVIIHAQAARQDQLEEMAAGNIIPSFFAAHPFYWGDWHRDAVLGPERAAFISPLAAAKALGIPYTVHNDPPVVPP
DVIRLLWVAVNRETRSGEILGPDQRAGPMDALRAVTLNAARQYFEEDEKGSITPGKKADFVVLSDNPLTIDPASMRDIRI
LETIKEGVTIYSAELPEDK
>Mature_578_residues
PSRARTGLWRSLVVAGALLAGCAAPQALSGEGAGEGATVFLNARIITVDETRPEAEAVAVKDGRILAVGTREEVTRMAGA
GAALRDLGGATLAPGFIDTHGHFALMAQAAGMANIQPPPAGPVRTIPELQAELEIWSGRHPDAAWITGWGYDDSLLAEQR
HPTRQDLDAVSTEKPVMLMHTSAHFAVCNTRCLEILGLTAETPDPSGGVIRREPDGRTPNGVLEESALYLLYQHLSQPGE
SQRIAGILETQTLYARYGITTVQEGAARPQNIQDLKKVAGQGGLYLDVVAYQLFPDGAMIAEDFTVSKTYENRFRVGGIK
LLLDGSLQGKTGWITKPYVSPPAGQAEGYSGYRIHTDESVAALFEAAYSRDIQIIAHANGDRAIDQYLDMIAPVIAAHPE
QDARPVIIHAQAARQDQLEEMAAGNIIPSFFAAHPFYWGDWHRDAVLGPERAAFISPLAAAKALGIPYTVHNDPPVVPPD
VIRLLWVAVNRETRSGEILGPDQRAGPMDALRAVTLNAARQYFEEDEKGSITPGKKADFVVLSDNPLTIDPASMRDIRIL
ETIKEGVTIYSAELPEDK

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 62416; Mature: 62285

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRARTGLWRSLVVAGALLAGCAAPQALSGEGAGEGATVFLNARIITVDETRPEAEAVA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEE
VKDGRILAVGTREEVTRMAGAGAALRDLGGATLAPGFIDTHGHFALMAQAAGMANIQPPP
EECCEEEEECCHHHHHHHHCCCHHHHHCCCCEECCCCEECCCCEEEEEHHCCCCCCCCCC
AGPVRTIPELQAELEIWSGRHPDAAWITGWGYDDSLLAEQRHPTRQDLDAVSTEKPVMLM
CCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHCCCCCEEEE
HTSAHFAVCNTRCLEILGLTAETPDPSGGVIRREPDGRTPNGVLEESALYLLYQHLSQPG
ECCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
ESQRIAGILETQTLYARYGITTVQEGAARPQNIQDLKKVAGQGGLYLDVVAYQLFPDGAM
CCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCE
IAEDFTVSKTYENRFRVGGIKLLLDGSLQGKTGWITKPYVSPPAGQAEGYSGYRIHTDES
EECCCEEECHHHCCEEECCEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEEECCHH
VAALFEAAYSRDIQIIAHANGDRAIDQYLDMIAPVIAAHPEQDARPVIIHAQAARQDQLE
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHH
EMAAGNIIPSFFAAHPFYWGDWHRDAVLGPERAAFISPLAAAKALGIPYTVHNDPPVVPP
HHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCH
DVIRLLWVAVNRETRSGEILGPDQRAGPMDALRAVTLNAARQYFEEDEKGSITPGKKADF
HHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VVLSDNPLTIDPASMRDIRILETIKEGVTIYSAELPEDK
EEECCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCCC
>Mature Secondary Structure 
PSRARTGLWRSLVVAGALLAGCAAPQALSGEGAGEGATVFLNARIITVDETRPEAEAVA
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCEEEE
VKDGRILAVGTREEVTRMAGAGAALRDLGGATLAPGFIDTHGHFALMAQAAGMANIQPPP
EECCEEEEECCHHHHHHHHCCCHHHHHCCCCEECCCCEECCCCEEEEEHHCCCCCCCCCC
AGPVRTIPELQAELEIWSGRHPDAAWITGWGYDDSLLAEQRHPTRQDLDAVSTEKPVMLM
CCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHCCCCCEEEE
HTSAHFAVCNTRCLEILGLTAETPDPSGGVIRREPDGRTPNGVLEESALYLLYQHLSQPG
ECCCCEEHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCC
ESQRIAGILETQTLYARYGITTVQEGAARPQNIQDLKKVAGQGGLYLDVVAYQLFPDGAM
CCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCE
IAEDFTVSKTYENRFRVGGIKLLLDGSLQGKTGWITKPYVSPPAGQAEGYSGYRIHTDES
EECCCEEECHHHCCEEECCEEEEEECCCCCCCCCEECCCCCCCCCCCCCCCCEEEECCHH
VAALFEAAYSRDIQIIAHANGDRAIDQYLDMIAPVIAAHPEQDARPVIIHAQAARQDQLE
HHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHH
EMAAGNIIPSFFAAHPFYWGDWHRDAVLGPERAAFISPLAAAKALGIPYTVHNDPPVVPP
HHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCH
DVIRLLWVAVNRETRSGEILGPDQRAGPMDALRAVTLNAARQYFEEDEKGSITPGKKADF
HHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VVLSDNPLTIDPASMRDIRILETIKEGVTIYSAELPEDK
EEECCCCEEECCCCCCHHHHHHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA