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The map label for this gene is folA [H]

Identifier: 114797071

GI number: 114797071

Start: 457456

End: 457986

Strand: Direct

Name: folA [H]

Synonym: HNE_0463

Alternate gene names: 114797071

Gene position: 457456-457986 (Clockwise)

Preceding gene: 114798833

Following gene: 114799910

Centisome position: 12.35

GC content: 64.97

Gene sequence:

>531_bases
ATGTCCATTGAAGTGAAACTTTGCCTGATCGTTGCGCAGGCGCGCAACCGGATCATCGGCGCCGCTGGCGCGCTGCCCTG
GCGGCTGAAGGACGATATGGCGTTCTTCAAGGCCACCACCATGGGCGCGCCCGTCATCATGGGCCGCAAGACCTGGGAAA
GCTTCCCCAAACGCCCCCTCCCTGGCCGCGAGAATATCGTGCTCACCCGCGACTGGGATTATGACGCCGCCGAAGCGCGC
GTTTACTCCAGCTTCCCCGCCGCCATGAACGCCGCCCGCGCCATCGCGGCCCGCGAAGGCAAGGACGAAGCCTTCGTCAT
CGGCGGCGCTGAAATCTACGCTCTCGCGCTGCCTTTCGCCGACCGGATCTACCTCACTGAGGTAGATGCCGAGATTGAAG
GCGACACGCTTTTCCCCGAACTCGACCTGCGCCAGTGGCGCAGCGATGAAATCGCCACCCATCCGGCCGGGGAGGGCAAC
GACCATGCCTTCATCATCCGCCGGCTCGACCGCGCCGCCGCTCAGGTCTGA

Upstream 100 bases:

>100_bases
CGCTGCGTTGCGTGCTTGCTTAAACCATGCCTGCGGAGCGAGGAAGCGCGCAAATGGGAACGCGCCAACCGGACAGATGT
GAAAGATAATAACTAGAGCA

Downstream 100 bases:

>100_bases
TCCGGGCCGCTTCACGCGGGGGCAGTGATGGACACTGTCCCCGCTGCGGCCTTACCCTTCCGGGAAAGATCATTCTCGGG
AGGAATAGAGTATGGATTTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 175

Protein sequence:

>176_residues
MSIEVKLCLIVAQARNRIIGAAGALPWRLKDDMAFFKATTMGAPVIMGRKTWESFPKRPLPGRENIVLTRDWDYDAAEAR
VYSSFPAAMNAARAIAAREGKDEAFVIGGAEIYALALPFADRIYLTEVDAEIEGDTLFPELDLRQWRSDEIATHPAGEGN
DHAFIIRRLDRAAAQV

Sequences:

>Translated_176_residues
MSIEVKLCLIVAQARNRIIGAAGALPWRLKDDMAFFKATTMGAPVIMGRKTWESFPKRPLPGRENIVLTRDWDYDAAEAR
VYSSFPAAMNAARAIAAREGKDEAFVIGGAEIYALALPFADRIYLTEVDAEIEGDTLFPELDLRQWRSDEIATHPAGEGN
DHAFIIRRLDRAAAQV
>Mature_175_residues
SIEVKLCLIVAQARNRIIGAAGALPWRLKDDMAFFKATTMGAPVIMGRKTWESFPKRPLPGRENIVLTRDWDYDAAEARV
YSSFPAAMNAARAIAAREGKDEAFVIGGAEIYALALPFADRIYLTEVDAEIEGDTLFPELDLRQWRSDEIATHPAGEGND
HAFIIRRLDRAAAQV

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=154, Percent_Identity=33.1168831168831, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1786233, Length=165, Percent_Identity=43.030303030303, Blast_Score=124, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17505899, Length=152, Percent_Identity=38.8157894736842, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324810, Length=126, Percent_Identity=34.1269841269841, Blast_Score=72, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116008026, Length=146, Percent_Identity=33.5616438356164, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24647458, Length=146, Percent_Identity=33.5616438356164, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19536; Mature: 19404

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEVKLCLIVAQARNRIIGAAGALPWRLKDDMAFFKATTMGAPVIMGRKTWESFPKRPL
CCEEEEEEEEEEHHHCCEEECCCCCCEEECCCHHHHHHHCCCCCEEECCHHHHHCCCCCC
PGRENIVLTRDWDYDAAEARVYSSFPAAMNAARAIAAREGKDEAFVIGGAEIYALALPFA
CCCCCEEEEECCCCCHHHHEEHHCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCC
DRIYLTEVDAEIEGDTLFPELDLRQWRSDEIATHPAGEGNDHAFIIRRLDRAAAQV
CEEEEEEECCEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCC
>Mature Secondary Structure 
SIEVKLCLIVAQARNRIIGAAGALPWRLKDDMAFFKATTMGAPVIMGRKTWESFPKRPL
CEEEEEEEEEEHHHCCEEECCCCCCEEECCCHHHHHHHCCCCCEEECCHHHHHCCCCCC
PGRENIVLTRDWDYDAAEARVYSSFPAAMNAARAIAAREGKDEAFVIGGAEIYALALPFA
CCCCCEEEEECCCCCHHHHEEHHCCCHHHHHHHHHHHCCCCCCEEEEECCEEEEEECCCC
DRIYLTEVDAEIEGDTLFPELDLRQWRSDEIATHPAGEGNDHAFIIRRLDRAAAQV
CEEEEEEECCEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]