Click here to switch to the map view.

The map label for this gene is asnB [H]

Identifier: 114331715

GI number: 114331715

Start: 1833019

End: 1834839

Strand: Direct

Name: asnB [H]

Synonym: Neut_1735

Alternate gene names: 114331715

Gene position: 1833019-1834839 (Clockwise)

Preceding gene: 114331714

Following gene: 114331716

Centisome position: 68.88

GC content: 56.73

Gene sequence:

>1821_bases
ATGAGCGGAATCGCTGGGTTCGTTGGGCGCGAGACTCCGGCGGCCTTGCGCATTAGGGTAGTCGAGGACATGGTGCGAAC
CGATGCCATGCAACGGCGCGGGCCGGACAGCCGAGGTACGGTGGTCGCGGATGAACGGGTCGTACTGGGCAATACTCGTC
TGCGCGTGGCCGATATACACAATTCTGAGGCAGACATGCCAATGCAGAGTGCCGACGGCATGCTGACCCTGACCTTCAAC
GGTGAAATCTACAATCATCTCGATCTGCGGCGCTTGTGTCCGGACTATCACTTCCGTACCAAATCGGATACCGAGAGCCT
GCTTGCCATCTACCAACGTCATGGCCTGGACATGCTAAGCATGCTCGAGGGCATGTTTGCCTTCTGTCTGCACGATCGGT
CGGCCAGACGAACGCTGCTGGCTGTGGATCCAACGGGACAAAAGCCACTGTACTACACGCTTGGGGACGGGGACGAACTG
TATTTTGCCTCGGATATTGAGACCATTTTGCGTATCCCTCATCTCAACTTCAGCTGGGACCGCGAAGGTTTGGCCCAGGC
CGCGGGCATCATGTTCGTATTTGGCCCCCATACTCATATCCGCGAGATCAAGAAGCTTGAGGCTGGATGTTGCCTGGTCG
TCGAACAGGGTGAACGCCCGCGTCATCGGCGCTATCACAAGATCGTCATCGGTGATGAATTGCAACAGGATGTCACCGCA
ATCACCGAACGTATTCGGGACGCAGTGGCTGCAGGCTGCACACGCTGTTTCGACCTGGAGGTTCCGTATGCCTATTTATT
AAGCGGCGGCATTGATTCGTCCAGCGTGGTAGCCCGAGCTAGGAGCCAGGGCTTGGAACTGCACACCTATGCTATCGGTT
TTCCCGAATCTGGGTTGAGGGCAGGTGCCATCATGCATAACGAGTTCAGCTACTCTCGGCTGGTGGCCGAAGCCTTCGGC
ACGGAGCACCGGGAAATTGAGGTGACGAGCCAGATCTATTGCGAGGCCATGGACCGTTGGTGGTCGCTGTGCTGCGAACC
GAATGGTGCACCCGAGGCGGTCTGTCTGTTCCTGCTGCTCGAGCAAATCGCCGCCGACGGTTATCGCGTAGCGTTTTGTG
GCAGCGGGCCGGACGAGGTATTCGATGGTTACAGTCATGGACACAGGCTGAAAATCACCCCCTCGAGGCTGGTAGCGGGT
GCCTATTTCGATTATTTCAATGCGGTGGGGGATATCGACCTCGGGCAACTGATGCTTGAGGTCGATGCTCGCGCCGCCTT
CATTTCCGCCGTGCAGCCCTATCTCGATCTGTACGCGCATGATACGCAGGACAGCCAACAGCTCACTTGCCTGCTCGATT
ATCACGGCGAGTGCGTAGTCTCTGAATATCGTCAGATAGACATGGCCAGCATGGCGCACAGTATTGAAGTCAGAATCCCA
CTAGCCGACCAGCAGGTCATTCAAAGCGCGTTTTCCTTTCTACCGAGGCTCAAGCACTATGAGGATTCAGAAAAATGGAT
CTTCAAGCAGGCGATGGCACCCTGGCTCCCTAGCGCAATCTCGAGCCGAAAGAAGATTGGCTTTCCAGTGCCGCAGATGG
TATATGCATCGCCCGAGTTTGAGGCGCGTATGGAGAGTTCGCTGGAGCCGGATAGCGGCCTTGATAAGCTTGGTCTGTTT
GACATGGCCTATCTCCGACGGGCTTGGCGCGAGCGAGGGAGCCGCGGCTATGACATCTTCTACCGCTTGCACGGTATGGA
TGTCATCGCACGCCGCCACTCGTCTTACCTAAGTCCCAAAGATCCGACGGCATACCAGTGA

Upstream 100 bases:

>100_bases
AGTTGGATCGAGCAGATGCGTCCCGCGCTGTTCGAGGATTATGAGACACCCGAGGCAGAGCACGCCACCGTTGCAACCTC
TGCGACACTGGGGGCTTGAG

Downstream 100 bases:

>100_bases
TAAATACCGCTCAATTCAGCATAAATGGAGGCACCCAGAAAGACCAGCTCGATCTGATGTCAGTGATGTCTACGTTATCC
CTATGCGGATTACGACACCG

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MSGIAGFVGRETPAALRIRVVEDMVRTDAMQRRGPDSRGTVVADERVVLGNTRLRVADIHNSEADMPMQSADGMLTLTFN
GEIYNHLDLRRLCPDYHFRTKSDTESLLAIYQRHGLDMLSMLEGMFAFCLHDRSARRTLLAVDPTGQKPLYYTLGDGDEL
YFASDIETILRIPHLNFSWDREGLAQAAGIMFVFGPHTHIREIKKLEAGCCLVVEQGERPRHRRYHKIVIGDELQQDVTA
ITERIRDAVAAGCTRCFDLEVPYAYLLSGGIDSSSVVARARSQGLELHTYAIGFPESGLRAGAIMHNEFSYSRLVAEAFG
TEHREIEVTSQIYCEAMDRWWSLCCEPNGAPEAVCLFLLLEQIAADGYRVAFCGSGPDEVFDGYSHGHRLKITPSRLVAG
AYFDYFNAVGDIDLGQLMLEVDARAAFISAVQPYLDLYAHDTQDSQQLTCLLDYHGECVVSEYRQIDMASMAHSIEVRIP
LADQQVIQSAFSFLPRLKHYEDSEKWIFKQAMAPWLPSAISSRKKIGFPVPQMVYASPEFEARMESSLEPDSGLDKLGLF
DMAYLRRAWRERGSRGYDIFYRLHGMDVIARRHSSYLSPKDPTAYQ

Sequences:

>Translated_606_residues
MSGIAGFVGRETPAALRIRVVEDMVRTDAMQRRGPDSRGTVVADERVVLGNTRLRVADIHNSEADMPMQSADGMLTLTFN
GEIYNHLDLRRLCPDYHFRTKSDTESLLAIYQRHGLDMLSMLEGMFAFCLHDRSARRTLLAVDPTGQKPLYYTLGDGDEL
YFASDIETILRIPHLNFSWDREGLAQAAGIMFVFGPHTHIREIKKLEAGCCLVVEQGERPRHRRYHKIVIGDELQQDVTA
ITERIRDAVAAGCTRCFDLEVPYAYLLSGGIDSSSVVARARSQGLELHTYAIGFPESGLRAGAIMHNEFSYSRLVAEAFG
TEHREIEVTSQIYCEAMDRWWSLCCEPNGAPEAVCLFLLLEQIAADGYRVAFCGSGPDEVFDGYSHGHRLKITPSRLVAG
AYFDYFNAVGDIDLGQLMLEVDARAAFISAVQPYLDLYAHDTQDSQQLTCLLDYHGECVVSEYRQIDMASMAHSIEVRIP
LADQQVIQSAFSFLPRLKHYEDSEKWIFKQAMAPWLPSAISSRKKIGFPVPQMVYASPEFEARMESSLEPDSGLDKLGLF
DMAYLRRAWRERGSRGYDIFYRLHGMDVIARRHSSYLSPKDPTAYQ
>Mature_605_residues
SGIAGFVGRETPAALRIRVVEDMVRTDAMQRRGPDSRGTVVADERVVLGNTRLRVADIHNSEADMPMQSADGMLTLTFNG
EIYNHLDLRRLCPDYHFRTKSDTESLLAIYQRHGLDMLSMLEGMFAFCLHDRSARRTLLAVDPTGQKPLYYTLGDGDELY
FASDIETILRIPHLNFSWDREGLAQAAGIMFVFGPHTHIREIKKLEAGCCLVVEQGERPRHRRYHKIVIGDELQQDVTAI
TERIRDAVAAGCTRCFDLEVPYAYLLSGGIDSSSVVARARSQGLELHTYAIGFPESGLRAGAIMHNEFSYSRLVAEAFGT
EHREIEVTSQIYCEAMDRWWSLCCEPNGAPEAVCLFLLLEQIAADGYRVAFCGSGPDEVFDGYSHGHRLKITPSRLVAGA
YFDYFNAVGDIDLGQLMLEVDARAAFISAVQPYLDLYAHDTQDSQQLTCLLDYHGECVVSEYRQIDMASMAHSIEVRIPL
ADQQVIQSAFSFLPRLKHYEDSEKWIFKQAMAPWLPSAISSRKKIGFPVPQMVYASPEFEARMESSLEPDSGLDKLGLFD
MAYLRRAWRERGSRGYDIFYRLHGMDVIARRHSSYLSPKDPTAYQ

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=548, Percent_Identity=25.7299270072993, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6321563, Length=550, Percent_Identity=25.6363636363636, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325403, Length=573, Percent_Identity=24.78184991274, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI45553209, Length=380, Percent_Identity=27.1052631578947, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 68371; Mature: 68239

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIAGFVGRETPAALRIRVVEDMVRTDAMQRRGPDSRGTVVADERVVLGNTRLRVADIH
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCEEEEEECC
NSEADMPMQSADGMLTLTFNGEIYNHLDLRRLCPDYHFRTKSDTESLLAIYQRHGLDMLS
CCCCCCCCCCCCCEEEEEECCCEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHH
MLEGMFAFCLHDRSARRTLLAVDPTGQKPLYYTLGDGDELYFASDIETILRIPHLNFSWD
HHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCCCCCC
REGLAQAAGIMFVFGPHTHIREIKKLEAGCCLVVEQGERPRHRRYHKIVIGDELQQDVTA
HHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCHHCCEEEEECCHHHHHHHH
ITERIRDAVAAGCTRCFDLEVPYAYLLSGGIDSSSVVARARSQGLELHTYAIGFPESGLR
HHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
AGAIMHNEFSYSRLVAEAFGTEHREIEVTSQIYCEAMDRWWSLCCEPNGAPEAVCLFLLL
CCEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EQIAADGYRVAFCGSGPDEVFDGYSHGHRLKITPSRLVAGAYFDYFNAVGDIDLGQLMLE
HHHHCCCEEEEEECCCHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHH
VDARAAFISAVQPYLDLYAHDTQDSQQLTCLLDYHGECVVSEYRQIDMASMAHSIEVRIP
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEE
LADQQVIQSAFSFLPRLKHYEDSEKWIFKQAMAPWLPSAISSRKKIGFPVPQMVYASPEF
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHEECCCCH
EARMESSLEPDSGLDKLGLFDMAYLRRAWRERGSRGYDIFYRLHGMDVIARRHSSYLSPK
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCC
DPTAYQ
CCCCCC
>Mature Secondary Structure 
SGIAGFVGRETPAALRIRVVEDMVRTDAMQRRGPDSRGTVVADERVVLGNTRLRVADIH
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCCEEEEEECC
NSEADMPMQSADGMLTLTFNGEIYNHLDLRRLCPDYHFRTKSDTESLLAIYQRHGLDMLS
CCCCCCCCCCCCCEEEEEECCCEECCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHH
MLEGMFAFCLHDRSARRTLLAVDPTGQKPLYYTLGDGDELYFASDIETILRIPHLNFSWD
HHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCCCCCC
REGLAQAAGIMFVFGPHTHIREIKKLEAGCCLVVEQGERPRHRRYHKIVIGDELQQDVTA
HHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEEEECCCCCCHHCCEEEEECCHHHHHHHH
ITERIRDAVAAGCTRCFDLEVPYAYLLSGGIDSSSVVARARSQGLELHTYAIGFPESGLR
HHHHHHHHHHHHCHHEEECCCCHHHHHCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCC
AGAIMHNEFSYSRLVAEAFGTEHREIEVTSQIYCEAMDRWWSLCCEPNGAPEAVCLFLLL
CCEEEECCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
EQIAADGYRVAFCGSGPDEVFDGYSHGHRLKITPSRLVAGAYFDYFNAVGDIDLGQLMLE
HHHHCCCEEEEEECCCHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCHHHHHHH
VDARAAFISAVQPYLDLYAHDTQDSQQLTCLLDYHGECVVSEYRQIDMASMAHSIEVRIP
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEE
LADQQVIQSAFSFLPRLKHYEDSEKWIFKQAMAPWLPSAISSRKKIGFPVPQMVYASPEF
CCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHEECCCCH
EARMESSLEPDSGLDKLGLFDMAYLRRAWRERGSRGYDIFYRLHGMDVIARRHSSYLSPK
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHCCCCCC
DPTAYQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]