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The map label for this gene is phaD [H]

Identifier: 114331683

GI number: 114331683

Start: 1797542

End: 1799188

Strand: Direct

Name: phaD [H]

Synonym: Neut_1699

Alternate gene names: 114331683

Gene position: 1797542-1799188 (Clockwise)

Preceding gene: 114331682

Following gene: 114331684

Centisome position: 67.55

GC content: 49.79

Gene sequence:

>1647_bases
ATGAAACTGTCTCCAGAACATCTTGTTATCGCACCTATTCTGATTCCACTCATCGCTGGTGCATTACAACTTTTTCTTGA
TGATCGCGGACGTAAGCTGAAGGCTTCCCTATCCATTCTGTCAACTGTTGCTCTGCTGGCAATCTCGGTTCTGCTGCTAC
GTTTTACTCACACGGATGATGAAGCAAGTGGGGGTAAAATTGCTGTCTATCTGCTCGGTAACTGGCCGCCTCCATTCGCT
ATCAATCTGGTACTGGATCGCTTGTCCGCCATGATGCTGGCCCTGACTGCCACGCTTGCTCTGCCCACTTTAATATTTTC
AATGGGGCGTTGGCACAAGGCTGGAGTGCATTTTCATACGTTGTTCCTATTACTGTTGATGGGGTTGAATGGTGCATTCC
TGACCGGGGACTTGTTCAATCTCTTTGTATTTTTTGAAGTCATGCTGGCTGCATCCTACGGATTAGTATTACATGGATCG
GGCTTGCTCCGGGTAAAGGCGGGGTTACACTACATCGCCATCAACCTCGTCGCTTCACTCCTGTTCCTGATCGGGGTCGC
TCTGATTTATGGTGTTACCGGCACCCTCAACATGGCTGATCTCGCCATACGCATCCCACAGATCAATGAAACAAACCAAA
CGATGCTGGAATCCGGGGCAGCCATTCTGAGTGTGGCCTTCCTGATTAAAGCGGGCATGTGGCCATTGAGTTTCTGGTTG
CCCGAAACCTACGCCGTAGCGCCGGCACCATCAGCTGCCATCTTTGCCATCATGAGTAAAGTTGGTATTTATGTGTTGTT
ACGCTTGGCACTACTTTTTTTTGGAAGTGATCCCGGTTTTTCAGAAGGGCCAGGAGCACAGATACTTTTGTATGGTGGTC
TAGCAACCATTGGCTTTGGCACCGTCGGTGTCCTCGCTTCACAGACTCTGGGACGATTTGCTGGTTTCTCAGTGCTGATC
TCATCTGGCACATTGCTAGCAAGCATGGGTCTGGTACAAGCCGAGGTGACTGCCGGCGCTCTGTTTTATCTCGTGAGCTC
CACGTTGGCAATCAGCGCATTATTCATGCTCATCGAACTGGTTGAAAGAGGGCAAAACCTGGCAGCTAGCGTGCTGGCCG
TTACCATGGAAGCTTTCGGTTATCAGGATGAGGAAATAGAGAATGAGGAAATTGTAGGAGTGGCTATCCCTCGAACGATA
GCTGTTCTCGGCACTTGCTTTGCAGCTTGTGCCATTTTGCTAGTTGGCCTCCCCCCTCTTTCGGGTTTCATTGCCAAATT
CGTCATCCTCTCCGCTATATTTAGCCCTGATATAGCAAGCCAGATAGATATTATCCCGGCAACGTCCTGGTGGTTCATGG
CACTACTGATTCTTTCCGGATTAGCTACACTGATTGCCATGAGTCGCTCTGGCATCCATGCTTTCTGGGCACCCTCGGTC
GACACCACGCTGCCACGGGTACTGTTTATCGAAATCATACCGGTTGCTGTGTTGCTATGTACAACGCTTGTATTGACTAT
ACAAGCAGGGCCGGTAATGCGTTTCATGGAAGCAACAGCAATCAGCCTGCATGCACCTCAGCCTTATATTGAAGGTGTAA
TGAATGCGCCAACCATGCTGCCACCGCCAATGGAGGTAGTAGAATGA

Upstream 100 bases:

>100_bases
TTGTCATTGGCTTTGCTACAACAGCGCTGTTTCTGGTTATTCTGCTAGCTTCGCGCGGCCTGACAGGTACCGATCATGTA
GATGGCGAGGAATCGGACCG

Downstream 100 bases:

>100_bases
GCAGACTATTCCCTTATCCACTGCTTGTTGTTTCGTTGGTTATCACATGGCTGATGCTGGCTGGCTTCTCACTCAATCAG
TTTTTACTGGGTTTGATAAC

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MKLSPEHLVIAPILIPLIAGALQLFLDDRGRKLKASLSILSTVALLAISVLLLRFTHTDDEASGGKIAVYLLGNWPPPFA
INLVLDRLSAMMLALTATLALPTLIFSMGRWHKAGVHFHTLFLLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLVLHGS
GLLRVKAGLHYIAINLVASLLFLIGVALIYGVTGTLNMADLAIRIPQINETNQTMLESGAAILSVAFLIKAGMWPLSFWL
PETYAVAPAPSAAIFAIMSKVGIYVLLRLALLFFGSDPGFSEGPGAQILLYGGLATIGFGTVGVLASQTLGRFAGFSVLI
SSGTLLASMGLVQAEVTAGALFYLVSSTLAISALFMLIELVERGQNLAASVLAVTMEAFGYQDEEIENEEIVGVAIPRTI
AVLGTCFAACAILLVGLPPLSGFIAKFVILSAIFSPDIASQIDIIPATSWWFMALLILSGLATLIAMSRSGIHAFWAPSV
DTTLPRVLFIEIIPVAVLLCTTLVLTIQAGPVMRFMEATAISLHAPQPYIEGVMNAPTMLPPPMEVVE

Sequences:

>Translated_548_residues
MKLSPEHLVIAPILIPLIAGALQLFLDDRGRKLKASLSILSTVALLAISVLLLRFTHTDDEASGGKIAVYLLGNWPPPFA
INLVLDRLSAMMLALTATLALPTLIFSMGRWHKAGVHFHTLFLLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLVLHGS
GLLRVKAGLHYIAINLVASLLFLIGVALIYGVTGTLNMADLAIRIPQINETNQTMLESGAAILSVAFLIKAGMWPLSFWL
PETYAVAPAPSAAIFAIMSKVGIYVLLRLALLFFGSDPGFSEGPGAQILLYGGLATIGFGTVGVLASQTLGRFAGFSVLI
SSGTLLASMGLVQAEVTAGALFYLVSSTLAISALFMLIELVERGQNLAASVLAVTMEAFGYQDEEIENEEIVGVAIPRTI
AVLGTCFAACAILLVGLPPLSGFIAKFVILSAIFSPDIASQIDIIPATSWWFMALLILSGLATLIAMSRSGIHAFWAPSV
DTTLPRVLFIEIIPVAVLLCTTLVLTIQAGPVMRFMEATAISLHAPQPYIEGVMNAPTMLPPPMEVVE
>Mature_548_residues
MKLSPEHLVIAPILIPLIAGALQLFLDDRGRKLKASLSILSTVALLAISVLLLRFTHTDDEASGGKIAVYLLGNWPPPFA
INLVLDRLSAMMLALTATLALPTLIFSMGRWHKAGVHFHTLFLLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLVLHGS
GLLRVKAGLHYIAINLVASLLFLIGVALIYGVTGTLNMADLAIRIPQINETNQTMLESGAAILSVAFLIKAGMWPLSFWL
PETYAVAPAPSAAIFAIMSKVGIYVLLRLALLFFGSDPGFSEGPGAQILLYGGLATIGFGTVGVLASQTLGRFAGFSVLI
SSGTLLASMGLVQAEVTAGALFYLVSSTLAISALFMLIELVERGQNLAASVLAVTMEAFGYQDEEIENEEIVGVAIPRTI
AVLGTCFAACAILLVGLPPLSGFIAKFVILSAIFSPDIASQIDIIPATSWWFMALLILSGLATLIAMSRSGIHAFWAPSV
DTTLPRVLFIEIIPVAVLLCTTLVLTIQAGPVMRFMEATAISLHAPQPYIEGVMNAPTMLPPPMEVVE

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=371, Percent_Identity=27.2237196765499, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788827, Length=387, Percent_Identity=25.3229974160207, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1788829, Length=474, Percent_Identity=25.7383966244726, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 58252; Mature: 58252

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSPEHLVIAPILIPLIAGALQLFLDDRGRKLKASLSILSTVALLAISVLLLRFTHTDD
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EASGGKIAVYLLGNWPPPFAINLVLDRLSAMMLALTATLALPTLIFSMGRWHKAGVHFHT
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHH
LFLLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLVLHGSGLLRVKAGLHYIAINLVASL
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHHHHHHHHHH
LFLIGVALIYGVTGTLNMADLAIRIPQINETNQTMLESGAAILSVAFLIKAGMWPLSFWL
HHHHHHHHHHCCCCCCCHHHHEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHC
PETYAVAPAPSAAIFAIMSKVGIYVLLRLALLFFGSDPGFSEGPGAQILLYGGLATIGFG
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHH
TVGVLASQTLGRFAGFSVLISSGTLLASMGLVQAEVTAGALFYLVSSTLAISALFMLIEL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VERGQNLAASVLAVTMEAFGYQDEEIENEEIVGVAIPRTIAVLGTCFAACAILLVGLPPL
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCH
SGFIAKFVILSAIFSPDIASQIDIIPATSWWFMALLILSGLATLIAMSRSGIHAFWAPSV
HHHHHHHHHHHHHHCCCCHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DTTLPRVLFIEIIPVAVLLCTTLVLTIQAGPVMRFMEATAISLHAPQPYIEGVMNAPTML
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHHCCCCCC
PPPMEVVE
CCCHHHCC
>Mature Secondary Structure
MKLSPEHLVIAPILIPLIAGALQLFLDDRGRKLKASLSILSTVALLAISVLLLRFTHTDD
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EASGGKIAVYLLGNWPPPFAINLVLDRLSAMMLALTATLALPTLIFSMGRWHKAGVHFHT
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHH
LFLLLLMGLNGAFLTGDLFNLFVFFEVMLAASYGLVLHGSGLLRVKAGLHYIAINLVASL
HHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHHHHHHHHHH
LFLIGVALIYGVTGTLNMADLAIRIPQINETNQTMLESGAAILSVAFLIKAGMWPLSFWL
HHHHHHHHHHCCCCCCCHHHHEEECCCCCCCHHHHHHCCHHHHHHHHHHHCCCCHHHHHC
PETYAVAPAPSAAIFAIMSKVGIYVLLRLALLFFGSDPGFSEGPGAQILLYGGLATIGFG
CCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHH
TVGVLASQTLGRFAGFSVLISSGTLLASMGLVQAEVTAGALFYLVSSTLAISALFMLIEL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VERGQNLAASVLAVTMEAFGYQDEEIENEEIVGVAIPRTIAVLGTCFAACAILLVGLPPL
HHCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCH
SGFIAKFVILSAIFSPDIASQIDIIPATSWWFMALLILSGLATLIAMSRSGIHAFWAPSV
HHHHHHHHHHHHHHCCCCHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
DTTLPRVLFIEIIPVAVLLCTTLVLTIQAGPVMRFMEATAISLHAPQPYIEGVMNAPTML
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCHHHHHHHCCCCCC
PPPMEVVE
CCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]