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The map label for this gene is priA [H]

Identifier: 114331666

GI number: 114331666

Start: 1775993

End: 1778194

Strand: Direct

Name: priA [H]

Synonym: Neut_1682

Alternate gene names: 114331666

Gene position: 1775993-1778194 (Clockwise)

Preceding gene: 114331665

Following gene: 114331670

Centisome position: 66.74

GC content: 51.0

Gene sequence:

>2202_bases
ATGACAGTCATTCATGTTGCGCTTGATGTTCCGGTTAACCGGTTGTTTGACTACCTTGCTCCGAATGCCGATTCAGCCGA
TATTGGCCGGTGCGTACGCGTTCCTTTCGGCAACCGGCAACTCTCTGGTATTATCCTGTCCGTCAGCAATTTACCGTCCG
TTCCGGAGAGAAAACTAAAAACTGCCGGCCTGATCGATCAAGCAATCCCTCCCCTGCCCCAGTCGTTACTCGAACTATTC
GAGTTTTGCAGTCACTATTATCACCACCCGATCGGACAGGTTGTCATGAATGGCCTGCCTGCTTTGCTGCGAAAACCTGG
GTATACAACCAGCAGGATACCGAAACAGATCCAGGAATGGATACTGACTGATGCAGGCAGAATTATCACGCTTTCCGACA
TTCCCGCCAGATCCAAAACCAAACGTCAGCTGTTTTCCCTGCTACTGGAAAACGGAATTATTACAGCTGACATTTACAAA
GCAATGTCACCTCATTCCCGCAAGCTGCTGCATGAATTCAAGGATTTGGGGTGGGTTGAACAACGCACTGCCTTACCGGG
AAAATTTGCATTCAGTACAGTACCCACCCTAGCTCCAACCACAGAACAGGCGCATGCCATCGCCGAAATTCTCGCCAGAA
TAGGTACGTTTACCCCCTGGTTGCTTCATGGCATCACTGGCAGTGGCAAAACAGAAGTCTATCTGCAGGTGGCCGCTTCT
CTGTTGGCCCAACAAAAGCAGATTCTGGTTCTGGTGCCGGAAATTAATCTTACTCCGCAACTGGAAGCAACTTTCCGCAA
GCGATTTCCCAATACACCACTGGTTAGCTTGCACAGCGGATTGAATAGCAGCGAGCGTCTGCAAGGCTGGTTGCAAGCAC
AATGTGGGGAAGCCAACATTATTCTGGGTACACGCCTGGCAATTTTCACTCCCATGCCCAGGCCGGGCCTGATTATTGTC
GATGAAGAACAGGATCATTCTTTCAAGCAGCAGGATGGATTGCGCTACTCTGCACGTGATCTGGCGGTGTACCGTGCAAG
ACAGGCCAACATACCCATCGTTCTTGGCTCAGCCACCCCTTCCCTGGAAAGCTATCACCAGGCGCGTATCGGCCGCTACC
GGTTACTGCAGCTGCACGCGCGAGCAATCAGTCAGGCAGTACTGCCAGCTATTCATTGTATAGACATGCGTGTAACGCCC
GCACAGGAAAGATTGTCGGAGCCCATACTTGATGCATTACGCCAATCTCTTGATCAAAAACAGCAAAGTCTAGTATTTAT
CAATCGACGCGGCTATTCCCCGGCACTACTCTGCAAATCCTGTCGCTGGATTGCCACCTGCAAACGCTGCTCCAGCCGCC
TGGTTGTCCATTTGCGCAATCGACAACTGCGCTGTCACTATTGCGGTGATCAGCAGCCGATTACACCCGCATGCCCGCAA
TGCGGAGATCCAGATATTATGCCGTTTGGCCACGGTACACAACGGATTGAATCAGCGTTAATCAAACAATTCCCGAACGC
GCGCATTCTACGTGTTGATCGTGACAGCATCCGCCACAAAACCGCCTGGCAAACCATGCTTGATACGATTCACCAGGGAA
AGGTGGATATTCTGGTTGGCACCCAATTACTTGCAAAAGGACATGATTTCCCAAACCTTGCCCTGGTGTGTGTGCTCAAC
GCCGATTCATCCCTTTACAGCACTGATTTCCGCGCTGAAGAACATCTTTTTTCACAGCTGATGCAAGTAGCAGGACGTGC
CGGTCGCGCCCATATTCCTGGCACCGTTCTGATCCAAACTGAATTTCCGCAGCATCCGCTGTACTTGGCATTAATACGTC
AGGATTATGCAGCATACGCGCAAACGCAGCTGAAAGAACGCAAATCAGCCAGTTTTCCACCTTTTGTCTATCTGGCAGTA
TTACGTGCAGAAGCCCCGGTACTCGCAGAAGCCTTGGAGTTTTTACACCAGACGGCGGTAATCGCCACTATTGCTAAAGA
ATACGCCCACATCCAGCTTTTCGATCCTGTTCCCGCTACCATGACTCGGCTCAAGGGACTGGAGCGTGCACAGCTGCTGA
TTCAGGCTGTTTCACGCAAGTGCCTGCAAATATTTCTGAGTGATTGGCACCAGCGTATTACTGCATTACCAGCTCGCAGC
AAGCTGCGCTGGCATTTGGATGTTGATCCACTGACGCTTTAA

Upstream 100 bases:

>100_bases
ACATTGCACCGCATCTCTCACTGATTGATTTTCCAAGAAACTCCGAAAGAATATCTGATCACGTTATTCTTTCGGAAAGT
TGATTCCGGATATTTCCGGT

Downstream 100 bases:

>100_bases
AAACAGCCCCCAGCACCGGGATCGGTTCGCTCACAGTGATTTTTCAGAGTACTCTAATCTGTATTTTCTCAATCCCTGGT
ATCGCGAACCAACAATATGG

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 733; Mature: 732

Protein sequence:

>733_residues
MTVIHVALDVPVNRLFDYLAPNADSADIGRCVRVPFGNRQLSGIILSVSNLPSVPERKLKTAGLIDQAIPPLPQSLLELF
EFCSHYYHHPIGQVVMNGLPALLRKPGYTTSRIPKQIQEWILTDAGRIITLSDIPARSKTKRQLFSLLLENGIITADIYK
AMSPHSRKLLHEFKDLGWVEQRTALPGKFAFSTVPTLAPTTEQAHAIAEILARIGTFTPWLLHGITGSGKTEVYLQVAAS
LLAQQKQILVLVPEINLTPQLEATFRKRFPNTPLVSLHSGLNSSERLQGWLQAQCGEANIILGTRLAIFTPMPRPGLIIV
DEEQDHSFKQQDGLRYSARDLAVYRARQANIPIVLGSATPSLESYHQARIGRYRLLQLHARAISQAVLPAIHCIDMRVTP
AQERLSEPILDALRQSLDQKQQSLVFINRRGYSPALLCKSCRWIATCKRCSSRLVVHLRNRQLRCHYCGDQQPITPACPQ
CGDPDIMPFGHGTQRIESALIKQFPNARILRVDRDSIRHKTAWQTMLDTIHQGKVDILVGTQLLAKGHDFPNLALVCVLN
ADSSLYSTDFRAEEHLFSQLMQVAGRAGRAHIPGTVLIQTEFPQHPLYLALIRQDYAAYAQTQLKERKSASFPPFVYLAV
LRAEAPVLAEALEFLHQTAVIATIAKEYAHIQLFDPVPATMTRLKGLERAQLLIQAVSRKCLQIFLSDWHQRITALPARS
KLRWHLDVDPLTL

Sequences:

>Translated_733_residues
MTVIHVALDVPVNRLFDYLAPNADSADIGRCVRVPFGNRQLSGIILSVSNLPSVPERKLKTAGLIDQAIPPLPQSLLELF
EFCSHYYHHPIGQVVMNGLPALLRKPGYTTSRIPKQIQEWILTDAGRIITLSDIPARSKTKRQLFSLLLENGIITADIYK
AMSPHSRKLLHEFKDLGWVEQRTALPGKFAFSTVPTLAPTTEQAHAIAEILARIGTFTPWLLHGITGSGKTEVYLQVAAS
LLAQQKQILVLVPEINLTPQLEATFRKRFPNTPLVSLHSGLNSSERLQGWLQAQCGEANIILGTRLAIFTPMPRPGLIIV
DEEQDHSFKQQDGLRYSARDLAVYRARQANIPIVLGSATPSLESYHQARIGRYRLLQLHARAISQAVLPAIHCIDMRVTP
AQERLSEPILDALRQSLDQKQQSLVFINRRGYSPALLCKSCRWIATCKRCSSRLVVHLRNRQLRCHYCGDQQPITPACPQ
CGDPDIMPFGHGTQRIESALIKQFPNARILRVDRDSIRHKTAWQTMLDTIHQGKVDILVGTQLLAKGHDFPNLALVCVLN
ADSSLYSTDFRAEEHLFSQLMQVAGRAGRAHIPGTVLIQTEFPQHPLYLALIRQDYAAYAQTQLKERKSASFPPFVYLAV
LRAEAPVLAEALEFLHQTAVIATIAKEYAHIQLFDPVPATMTRLKGLERAQLLIQAVSRKCLQIFLSDWHQRITALPARS
KLRWHLDVDPLTL
>Mature_732_residues
TVIHVALDVPVNRLFDYLAPNADSADIGRCVRVPFGNRQLSGIILSVSNLPSVPERKLKTAGLIDQAIPPLPQSLLELFE
FCSHYYHHPIGQVVMNGLPALLRKPGYTTSRIPKQIQEWILTDAGRIITLSDIPARSKTKRQLFSLLLENGIITADIYKA
MSPHSRKLLHEFKDLGWVEQRTALPGKFAFSTVPTLAPTTEQAHAIAEILARIGTFTPWLLHGITGSGKTEVYLQVAASL
LAQQKQILVLVPEINLTPQLEATFRKRFPNTPLVSLHSGLNSSERLQGWLQAQCGEANIILGTRLAIFTPMPRPGLIIVD
EEQDHSFKQQDGLRYSARDLAVYRARQANIPIVLGSATPSLESYHQARIGRYRLLQLHARAISQAVLPAIHCIDMRVTPA
QERLSEPILDALRQSLDQKQQSLVFINRRGYSPALLCKSCRWIATCKRCSSRLVVHLRNRQLRCHYCGDQQPITPACPQC
GDPDIMPFGHGTQRIESALIKQFPNARILRVDRDSIRHKTAWQTMLDTIHQGKVDILVGTQLLAKGHDFPNLALVCVLNA
DSSLYSTDFRAEEHLFSQLMQVAGRAGRAHIPGTVLIQTEFPQHPLYLALIRQDYAAYAQTQLKERKSASFPPFVYLAVL
RAEAPVLAEALEFLHQTAVIATIAKEYAHIQLFDPVPATMTRLKGLERAQLLIQAVSRKCLQIFLSDWHQRITALPARSK
LRWHLDVDPLTL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=740, Percent_Identity=41.7567567567568, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1787357, Length=187, Percent_Identity=33.1550802139037, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82150; Mature: 82018

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIHVALDVPVNRLFDYLAPNADSADIGRCVRVPFGNRQLSGIILSVSNLPSVPERKLK
CEEEEEEECCCHHHHHHHHCCCCCCCCCCCEEECCCCCCEECEEEEEECCCCCCCHHHHH
TAGLIDQAIPPLPQSLLELFEFCSHYYHHPIGQVVMNGLPALLRKPGYTTSRIPKQIQEW
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHH
ILTDAGRIITLSDIPARSKTKRQLFSLLLENGIITADIYKAMSPHSRKLLHEFKDLGWVE
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEHHHHHHCCCHHHHHHHHHHHCCCHH
QRTALPGKFAFSTVPTLAPTTEQAHAIAEILARIGTFTPWLLHGITGSGKTEVYLQVAAS
HHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHH
LLAQQKQILVLVPEINLTPQLEATFRKRFPNTPLVSLHSGLNSSERLQGWLQAQCGEANI
HHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHHHCCCCCEE
ILGTRLAIFTPMPRPGLIIVDEEQDHSFKQQDGLRYSARDLAVYRARQANIPIVLGSATP
EEECEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCC
SLESYHQARIGRYRLLQLHARAISQAVLPAIHCIDMRVTPAQERLSEPILDALRQSLDQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
QQSLVFINRRGYSPALLCKSCRWIATCKRCSSRLVVHLRNRQLRCHYCGDQQPITPACPQ
HHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCCCCCCC
CGDPDIMPFGHGTQRIESALIKQFPNARILRVDRDSIRHKTAWQTMLDTIHQGKVDILVG
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEC
TQLLAKGHDFPNLALVCVLNADSSLYSTDFRAEEHLFSQLMQVAGRAGRAHIPGTVLIQT
HHHHHCCCCCCCEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
EFPQHPLYLALIRQDYAAYAQTQLKERKSASFPPFVYLAVLRAEAPVLAEALEFLHQTAV
CCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
IATIAKEYAHIQLFDPVPATMTRLKGLERAQLLIQAVSRKCLQIFLSDWHQRITALPARS
HHHHHHHHCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KLRWHLDVDPLTL
CEEEEECCCCCCC
>Mature Secondary Structure 
TVIHVALDVPVNRLFDYLAPNADSADIGRCVRVPFGNRQLSGIILSVSNLPSVPERKLK
EEEEEEECCCHHHHHHHHCCCCCCCCCCCEEECCCCCCEECEEEEEECCCCCCCHHHHH
TAGLIDQAIPPLPQSLLELFEFCSHYYHHPIGQVVMNGLPALLRKPGYTTSRIPKQIQEW
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHH
ILTDAGRIITLSDIPARSKTKRQLFSLLLENGIITADIYKAMSPHSRKLLHEFKDLGWVE
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEHHHHHHCCCHHHHHHHHHHHCCCHH
QRTALPGKFAFSTVPTLAPTTEQAHAIAEILARIGTFTPWLLHGITGSGKTEVYLQVAAS
HHHCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHH
LLAQQKQILVLVPEINLTPQLEATFRKRFPNTPLVSLHSGLNSSERLQGWLQAQCGEANI
HHHCCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHHHCCCCCEE
ILGTRLAIFTPMPRPGLIIVDEEQDHSFKQQDGLRYSARDLAVYRARQANIPIVLGSATP
EEECEEEEECCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCC
SLESYHQARIGRYRLLQLHARAISQAVLPAIHCIDMRVTPAQERLSEPILDALRQSLDQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
QQSLVFINRRGYSPALLCKSCRWIATCKRCSSRLVVHLRNRQLRCHYCGDQQPITPACPQ
HHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCCCCCCCCCCC
CGDPDIMPFGHGTQRIESALIKQFPNARILRVDRDSIRHKTAWQTMLDTIHQGKVDILVG
CCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEC
TQLLAKGHDFPNLALVCVLNADSSLYSTDFRAEEHLFSQLMQVAGRAGRAHIPGTVLIQT
HHHHHCCCCCCCEEEEEEECCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
EFPQHPLYLALIRQDYAAYAQTQLKERKSASFPPFVYLAVLRAEAPVLAEALEFLHQTAV
CCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
IATIAKEYAHIQLFDPVPATMTRLKGLERAQLLIQAVSRKCLQIFLSDWHQRITALPARS
HHHHHHHHCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KLRWHLDVDPLTL
CEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]