Click here to switch to the map view.

The map label for this gene is selU

Identifier: 114331640

GI number: 114331640

Start: 1739115

End: 1740281

Strand: Direct

Name: selU

Synonym: Neut_1655

Alternate gene names: 114331640

Gene position: 1739115-1740281 (Clockwise)

Preceding gene: 114331639

Following gene: 114331646

Centisome position: 65.35

GC content: 48.59

Gene sequence:

>1167_bases
TTGAATAATCCAAACATTGATAGCGATGAGCTAACAGCTTTATTCACAGCGGATACGCCACTGATTGACGTACGTGCACC
GCTTGAATTCATACTGGGATCCCTGCCCGGCTCAGTCAATCTGCCCATCCTCAACAACGAAGAACGTGCACTGGTCGGCA
CTACTTACAAACAGCAAGGTTCCGAGGCAGCAGTCATGCTTGGCCATGAGATGATTTCGGGTCAGGCAAAACAGGATCGT
CTGCAGCAATGGCTTGATTTTATCCGGCAACATCCCCGAACCGTGCTGTATTGCTTTCGAGGAGGAAAGCGCTCGCAGAT
CACGCAACAATGGTTGAAAGATGCTGGTGTCGACAGGCCATTGATCATCGGTGGTTATAAGCAGGCACGCCAATTTTTGA
TCAACACAATTGACCGGTTCAGTGAAAACCACACGCTACTGGTCATTACCGGACCAACGGGCAGTGGCAAAACCCAGCTA
ATCCAGGAGATCAGAAACAGTTACCCGGTACTTGACATCGAAGCCCTTGCCCGGCATCGTGGCTCGGCTTTTGGAGGAAT
GTCTCTCCCCCAGCCCACACAAATCGATTTTGAGAATCAGTTGGCTGTCAATTTGCTCAAACTGAAACAGAACTATCTGT
TTGATCCAGTCGTTGTGGAAGACGAAAGCCGACATACCGGCAGGGTATATTTACCAGCCAGCTTCTTTGAACACATGCGC
AGCTCTGAAATCATCTGGGTGGATGAACCATTGAATACCAGGGTAGATAACATATTCGGAGATTACATTGTTGCTACCCC
TATCGGCCAGGCACAGCAGGCTCGGCGCAACACACAACCATTGTCTTCTGCTACGGAAATTCAGGAAATACTGTGCCAAC
AAGCGCTGTTATTATTCGACAAATACGCAAGTGCCTTGCAGGCAATCAGCAAAAAACTGGGAGGAGTTAGGTTTCAGGAA
GTATCACAGGATCTGGAAAACGCCCGATCCAGCTTTGTAAACAGAAATGAAATTCAATCAAACAAGACCTGGATTGAAAA
ACTGGTGAGATATTACTATGACCCACTGTATCTCGGCTCACTACAACGCCGCCAGGTCAATCCCTGCTTCAAGGGCTCAA
GGCAGGCAGCAATCGATTATCTGCGGGCTCGCAAACAACACGCTTGA

Upstream 100 bases:

>100_bases
CCCGAAGCCAGCCAGCTCGTGCTGCAGGCACTGCGCACATACTTCAAGCCAGCAGCAATAGTGGGAACCGTTCATCCTCG
TCAAGATAAAGCGGTGCAAT

Downstream 100 bases:

>100_bases
CGTATCTCCTTCCGCTTGCCACCCCAAATGCCATGGATGATTACCAGCATGGCGGAAGAAAGTGCCACAAAATCGTTCAG
AATACTCGCAGATAACAGTA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNNPNIDSDELTALFTADTPLIDVRAPLEFILGSLPGSVNLPILNNEERALVGTTYKQQGSEAAVMLGHEMISGQAKQDR
LQQWLDFIRQHPRTVLYCFRGGKRSQITQQWLKDAGVDRPLIIGGYKQARQFLINTIDRFSENHTLLVITGPTGSGKTQL
IQEIRNSYPVLDIEALARHRGSAFGGMSLPQPTQIDFENQLAVNLLKLKQNYLFDPVVVEDESRHTGRVYLPASFFEHMR
SSEIIWVDEPLNTRVDNIFGDYIVATPIGQAQQARRNTQPLSSATEIQEILCQQALLLFDKYASALQAISKKLGGVRFQE
VSQDLENARSSFVNRNEIQSNKTWIEKLVRYYYDPLYLGSLQRRQVNPCFKGSRQAAIDYLRARKQHA

Sequences:

>Translated_388_residues
MNNPNIDSDELTALFTADTPLIDVRAPLEFILGSLPGSVNLPILNNEERALVGTTYKQQGSEAAVMLGHEMISGQAKQDR
LQQWLDFIRQHPRTVLYCFRGGKRSQITQQWLKDAGVDRPLIIGGYKQARQFLINTIDRFSENHTLLVITGPTGSGKTQL
IQEIRNSYPVLDIEALARHRGSAFGGMSLPQPTQIDFENQLAVNLLKLKQNYLFDPVVVEDESRHTGRVYLPASFFEHMR
SSEIIWVDEPLNTRVDNIFGDYIVATPIGQAQQARRNTQPLSSATEIQEILCQQALLLFDKYASALQAISKKLGGVRFQE
VSQDLENARSSFVNRNEIQSNKTWIEKLVRYYYDPLYLGSLQRRQVNPCFKGSRQAAIDYLRARKQHA
>Mature_388_residues
MNNPNIDSDELTALFTADTPLIDVRAPLEFILGSLPGSVNLPILNNEERALVGTTYKQQGSEAAVMLGHEMISGQAKQDR
LQQWLDFIRQHPRTVLYCFRGGKRSQITQQWLKDAGVDRPLIIGGYKQARQFLINTIDRFSENHTLLVITGPTGSGKTQL
IQEIRNSYPVLDIEALARHRGSAFGGMSLPQPTQIDFENQLAVNLLKLKQNYLFDPVVVEDESRHTGRVYLPASFFEHMR
SSEIIWVDEPLNTRVDNIFGDYIVATPIGQAQQARRNTQPLSSATEIQEILCQQALLLFDKYASALQAISKKLGGVRFQE
VSQDLENARSSFVNRNEIQSNKTWIEKLVRYYYDPLYLGSLQRRQVNPCFKGSRQAAIDYLRARKQHA

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain

Homologues:

Organism=Escherichia coli, GI1786712, Length=372, Percent_Identity=37.6344086021505, Blast_Score=255, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SELU_NITEC (Q0AFI5)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747862.1
- ProteinModelPortal:   Q0AFI5
- STRING:   Q0AFI5
- GeneID:   4273808
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1655
- NMPDR:   fig|335283.3.peg.2204
- eggNOG:   COG2603
- HOGENOM:   HBG452364
- OMA:   LINSHEY
- PhylomeDB:   Q0AFI5
- ProtClustDB:   PRK11784
- BioCyc:   NEUT335283:NEUT_1655-MONOMER
- HAMAP:   MF_01622
- InterPro:   IPR001763
- InterPro:   IPR017582
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450
- TIGRFAMs:   TIGR03167

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: NA

Molecular weight: Translated: 44009; Mature: 44009

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50206 RHODANESE_3

Important sites: ACT_SITE 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPNIDSDELTALFTADTPLIDVRAPLEFILGSLPGSVNLPILNNEERALVGTTYKQQG
CCCCCCCHHHEEEEEECCCCEEECHHHHHHHHHCCCCCCCCCEECCCCCEEEECCHHHCC
SEAAVMLGHEMISGQAKQDRLQQWLDFIRQHPRTVLYCFRGGKRSQITQQWLKDAGVDRP
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
LIIGGYKQARQFLINTIDRFSENHTLLVITGPTGSGKTQLIQEIRNSYPVLDIEALARHR
EEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHHHHC
GSAFGGMSLPQPTQIDFENQLAVNLLKLKQNYLFDPVVVEDESRHTGRVYLPASFFEHMR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECHHHHHHHC
SSEIIWVDEPLNTRVDNIFGDYIVATPIGQAQQARRNTQPLSSATEIQEILCQQALLLFD
CCCEEEECCCCCHHHHHHHCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KYASALQAISKKLGGVRFQEVSQDLENARSSFVNRNEIQSNKTWIEKLVRYYYDPLYLGS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHCCHHHCC
LQRRQVNPCFKGSRQAAIDYLRARKQHA
HHHHCCCHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNPNIDSDELTALFTADTPLIDVRAPLEFILGSLPGSVNLPILNNEERALVGTTYKQQG
CCCCCCCHHHEEEEEECCCCEEECHHHHHHHHHCCCCCCCCCEECCCCCEEEECCHHHCC
SEAAVMLGHEMISGQAKQDRLQQWLDFIRQHPRTVLYCFRGGKRSQITQQWLKDAGVDRP
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCCC
LIIGGYKQARQFLINTIDRFSENHTLLVITGPTGSGKTQLIQEIRNSYPVLDIEALARHR
EEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEHHHHHHHC
GSAFGGMSLPQPTQIDFENQLAVNLLKLKQNYLFDPVVVEDESRHTGRVYLPASFFEHMR
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECHHHHHHHC
SSEIIWVDEPLNTRVDNIFGDYIVATPIGQAQQARRNTQPLSSATEIQEILCQQALLLFD
CCCEEEECCCCCHHHHHHHCCEEEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
KYASALQAISKKLGGVRFQEVSQDLENARSSFVNRNEIQSNKTWIEKLVRYYYDPLYLGS
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHCCHHHCC
LQRRQVNPCFKGSRQAAIDYLRARKQHA
HHHHCCCHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA