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The map label for this gene is mdh

Identifier: 114331619

GI number: 114331619

Start: 1718527

End: 1719510

Strand: Direct

Name: mdh

Synonym: Neut_1633

Alternate gene names: 114331619

Gene position: 1718527-1719510 (Clockwise)

Preceding gene: 114331616

Following gene: 114331635

Centisome position: 64.58

GC content: 46.24

Gene sequence:

>984_bases
GTGCTTTCACCCGTTCGTATTACCGTCACCGGCGCAGCAGGACAAATCAGCTACAGCCTGTTATTCCGAATTGCTGCGGG
AGACATGCTGGGCAGCAATCAACCCGTTATTCTTCAACTCCTCGACATCCCGGAATCCAGGAAAATACTGGATGGAGTGG
TTATGGAACTTCAGGATTGCGCATTTCCGCTACTGGCTGGCATTATTGCCACTCACGACCCGATGATGGCATTTGATCAG
ACAGATATAGCAATACTAGTTGGGGCCCGCCCCCGCCGCAAAGGGATGGAACGTAAGGATTTACTGCAGACCAATGGCGA
AATATTCAGAAATCAGGGCAAGATCCTGAATCAAGTGGCCAAACGTGATGCAAAAATACTGGTGGTGGGAAATCCAGCGA
ACACCAATACCCTTATCACCATGAAAAATGCTCCAGATTTACCAGCAGAAAATTTTTCAGGAATGCTCCGGCTCGATCAT
AACCGGGCATTATCACAAGTTGCTATGAAGTTAAACCAGCCGGTCAGCAATATCAAAAAGATGATTGTATGGGGAAACCA
TTCATCCACTCAATTTCCTGATTTGTATCATGCTGAGATCGGTGATACCAAAGTTATTAATCTCATCAAAGATCCGATTT
GGATCGAAAACTACTTTATCCCTACGGTACAAAAACGGGGTGCTGTGGTAATCGAGGCACGTGGCTTATCCAGTGCAGCC
AGTGCAGCTAATGCTATTATTGATCATCTGCGTAACTGGTTTTTTGGAACGGCCGACGGTGACTGGACTACGATGGGAAT
CTTATCCGATGGTAGTTATGAAATACCGGAGGGGGTAATTTATGGCTTCCCGGTCATCTGCAAAAATGGTATGAGGGAAA
TTGTACAGGGACTTGAAATCAACCCATTCTCACGTACCAGATTAGATGCTGCCTACAACGAGCTAACTCAGGAACTGGAT
TCAATCAGACACTTGCTACCCTGA

Upstream 100 bases:

>100_bases
TTCTTGTCAATTGAGTTTATCGGTATAATTCCTACCAATCGTTTTTACCAATTCTCTTTTCCTGTCAGGAAATATATCAA
ATACCAAGGAGCAGAATTTG

Downstream 100 bases:

>100_bases
GATTACGAAACTAGATTCTATAGTTTCCAAGCCTCTCAAGTGCTTATCACATTATATTTTCTTGATAAATGCTAAAACTT
CCTCAGCATGCCCTGGAACC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MLSPVRITVTGAAGQISYSLLFRIAAGDMLGSNQPVILQLLDIPESRKILDGVVMELQDCAFPLLAGIIATHDPMMAFDQ
TDIAILVGARPRRKGMERKDLLQTNGEIFRNQGKILNQVAKRDAKILVVGNPANTNTLITMKNAPDLPAENFSGMLRLDH
NRALSQVAMKLNQPVSNIKKMIVWGNHSSTQFPDLYHAEIGDTKVINLIKDPIWIENYFIPTVQKRGAVVIEARGLSSAA
SAANAIIDHLRNWFFGTADGDWTTMGILSDGSYEIPEGVIYGFPVICKNGMREIVQGLEINPFSRTRLDAAYNELTQELD
SIRHLLP

Sequences:

>Translated_327_residues
MLSPVRITVTGAAGQISYSLLFRIAAGDMLGSNQPVILQLLDIPESRKILDGVVMELQDCAFPLLAGIIATHDPMMAFDQ
TDIAILVGARPRRKGMERKDLLQTNGEIFRNQGKILNQVAKRDAKILVVGNPANTNTLITMKNAPDLPAENFSGMLRLDH
NRALSQVAMKLNQPVSNIKKMIVWGNHSSTQFPDLYHAEIGDTKVINLIKDPIWIENYFIPTVQKRGAVVIEARGLSSAA
SAANAIIDHLRNWFFGTADGDWTTMGILSDGSYEIPEGVIYGFPVICKNGMREIVQGLEINPFSRTRLDAAYNELTQELD
SIRHLLP
>Mature_327_residues
MLSPVRITVTGAAGQISYSLLFRIAAGDMLGSNQPVILQLLDIPESRKILDGVVMELQDCAFPLLAGIIATHDPMMAFDQ
TDIAILVGARPRRKGMERKDLLQTNGEIFRNQGKILNQVAKRDAKILVVGNPANTNTLITMKNAPDLPAENFSGMLRLDH
NRALSQVAMKLNQPVSNIKKMIVWGNHSSTQFPDLYHAEIGDTKVINLIKDPIWIENYFIPTVQKRGAVVIEARGLSSAA
SAANAIIDHLRNWFFGTADGDWTTMGILSDGSYEIPEGVIYGFPVICKNGMREIVQGLEINPFSRTRLDAAYNELTQELD
SIRHLLP

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=331, Percent_Identity=52.5679758308157, Blast_Score=363, Evalue=1e-101,
Organism=Homo sapiens, GI89886456, Length=330, Percent_Identity=24.8484848484848, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17561064, Length=325, Percent_Identity=51.6923076923077, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=54.1984732824427, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=47.6470588235294, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24583394, Length=330, Percent_Identity=52.4242424242424, Blast_Score=343, Evalue=7e-95,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_NITEC (Q0AFK6)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747841.1
- ProteinModelPortal:   Q0AFK6
- SMR:   Q0AFK6
- STRING:   Q0AFK6
- GeneID:   4272497
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1633
- NMPDR:   fig|335283.3.peg.1934
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   NEUT335283:NEUT_1633-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 36103; Mature: 36103

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPVRITVTGAAGQISYSLLFRIAAGDMLGSNQPVILQLLDIPESRKILDGVVMELQDC
CCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AFPLLAGIIATHDPMMAFDQTDIAILVGARPRRKGMERKDLLQTNGEIFRNQGKILNQVA
HHHHHHHHHHCCCCCEEECCCCEEEEECCCHHHHCCCHHHHHHCCCHHHHHCCHHHHHHH
KRDAKILVVGNPANTNTLITMKNAPDLPAENFSGMLRLDHNRALSQVAMKLNQPVSNIKK
HCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCHHHHEE
MIVWGNHSSTQFPDLYHAEIGDTKVINLIKDPIWIENYFIPTVQKRGAVVIEARGLSSAA
EEEECCCCCCCCCCCEECCCCCHHHHHHHCCCEEEECEECCCCCCCCEEEEEECCCCHHH
SAANAIIDHLRNWFFGTADGDWTTMGILSDGSYEIPEGVIYGFPVICKNGMREIVQGLEI
HHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCC
NPFSRTRLDAAYNELTQELDSIRHLLP
CCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSPVRITVTGAAGQISYSLLFRIAAGDMLGSNQPVILQLLDIPESRKILDGVVMELQDC
CCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
AFPLLAGIIATHDPMMAFDQTDIAILVGARPRRKGMERKDLLQTNGEIFRNQGKILNQVA
HHHHHHHHHHCCCCCEEECCCCEEEEECCCHHHHCCCHHHHHHCCCHHHHHCCHHHHHHH
KRDAKILVVGNPANTNTLITMKNAPDLPAENFSGMLRLDHNRALSQVAMKLNQPVSNIKK
HCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCHHHHEE
MIVWGNHSSTQFPDLYHAEIGDTKVINLIKDPIWIENYFIPTVQKRGAVVIEARGLSSAA
EEEECCCCCCCCCCCEECCCCCHHHHHHHCCCEEEECEECCCCCCCCEEEEEECCCCHHH
SAANAIIDHLRNWFFGTADGDWTTMGILSDGSYEIPEGVIYGFPVICKNGMREIVQGLEI
HHHHHHHHHHHHHEEECCCCCEEEEEEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCC
NPFSRTRLDAAYNELTQELDSIRHLLP
CCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA