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The map label for this gene is folD

Identifier: 114331593

GI number: 114331593

Start: 1688663

End: 1689550

Strand: Direct

Name: folD

Synonym: Neut_1607

Alternate gene names: 114331593

Gene position: 1688663-1689550 (Clockwise)

Preceding gene: 114331592

Following gene: 114331594

Centisome position: 63.46

GC content: 48.09

Gene sequence:

>888_bases
ATGAGTGCAACGATTATCAGCGGTAATTTTATCGCAAGCAAACTACGCGAAGAACTGAAACAACGTATCCGAATCCTCTC
GGAAACATGGATGCAACCCGGCCTGGCAGTTATTCTGGCAGGAGACAATCCGGCATCTTCCGTATATGTTCGCAACAAGG
CAAGAACCTGTGGAGAATTGGGTATCCACTCCAAGGTTTTTAATTTCTCTGCTGATACTCCCCAGAAAATTATATTACGG
CAGATACAGGATTTGAACGCTAACCCCGAAATTCACGGTATTCTGGTGCAATTGCCTTTACCCAGCCATATCCAGATGAA
TGAAGTCATTGCCGCCATAGCAATTGAAAAGGATGTAGACGGATTTCACCCCTGTAATGTAGGCGCATTGGCAACAGGAC
ACGCCTTGTTTCGTCCGTGCACTCCCTTTGGCGTCATGAAAATGCTGGCGGAATATGGCATCCCGCTTCAGGGGCAACAC
GCAGTTGTTGTCGGGCGCAGCAATATTGTCGGAAAACCGATGGCGCTCATGCTGCTGGAACAGGGCGCAACTGTCACAAT
TTGCACCTCACAAACTCGGGAACTAGCCAGCCACACCCGCAGCGCCGACATTATCGTAATGGCCACAGGCAAAGCAAATT
TACTGACAAGTGATATGATCAGGACAGGTGCAACTGTAATCGATGTCGGTATTAATCGTCTGGCAGATGGACAGCTTTGT
GGTGATGTCGAATTCTCAGGCGTAAAAGAAAAAGCAGGATATATCACACCGGTTCCAGGTGGAGTGGGACCGATGACTAT
CACAATGCTGATGAATAATACTATCGAAGCTGCGGAACACGCGAAAGCAAAAGCTCAGATTGAAGCTCGGTGCGGTTCTA
TGCAGTAA

Upstream 100 bases:

>100_bases
AATTTGCCAGGAAAAAAACATTTTGGCGAAAATTTCCTAAATCAGCCCTACTGATTTAAGCAGGCTATTTACCTGCTCTA
TTCGATATCTCCTGAATTTT

Downstream 100 bases:

>100_bases
TCTTGAACTTGTAGACTTGTAAACAGGGAGCAGACCCCAATATGGATCCACTGGATATCGACCCCCAGGAAACACAGGAA
TGGCTGGATGCCCTGGAAAC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSATIISGNFIASKLREELKQRIRILSETWMQPGLAVILAGDNPASSVYVRNKARTCGELGIHSKVFNFSADTPQKIILR
QIQDLNANPEIHGILVQLPLPSHIQMNEVIAAIAIEKDVDGFHPCNVGALATGHALFRPCTPFGVMKMLAEYGIPLQGQH
AVVVGRSNIVGKPMALMLLEQGATVTICTSQTRELASHTRSADIIVMATGKANLLTSDMIRTGATVIDVGINRLADGQLC
GDVEFSGVKEKAGYITPVPGGVGPMTITMLMNNTIEAAEHAKAKAQIEARCGSMQ

Sequences:

>Translated_295_residues
MSATIISGNFIASKLREELKQRIRILSETWMQPGLAVILAGDNPASSVYVRNKARTCGELGIHSKVFNFSADTPQKIILR
QIQDLNANPEIHGILVQLPLPSHIQMNEVIAAIAIEKDVDGFHPCNVGALATGHALFRPCTPFGVMKMLAEYGIPLQGQH
AVVVGRSNIVGKPMALMLLEQGATVTICTSQTRELASHTRSADIIVMATGKANLLTSDMIRTGATVIDVGINRLADGQLC
GDVEFSGVKEKAGYITPVPGGVGPMTITMLMNNTIEAAEHAKAKAQIEARCGSMQ
>Mature_294_residues
SATIISGNFIASKLREELKQRIRILSETWMQPGLAVILAGDNPASSVYVRNKARTCGELGIHSKVFNFSADTPQKIILRQ
IQDLNANPEIHGILVQLPLPSHIQMNEVIAAIAIEKDVDGFHPCNVGALATGHALFRPCTPFGVMKMLAEYGIPLQGQHA
VVVGRSNIVGKPMALMLLEQGATVTICTSQTRELASHTRSADIIVMATGKANLLTSDMIRTGATVIDVGINRLADGQLCG
DVEFSGVKEKAGYITPVPGGVGPMTITMLMNNTIEAAEHAKAKAQIEARCGSMQ

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=297, Percent_Identity=45.4545454545455, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=44.4444444444444, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.4137931034483, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI36796743, Length=229, Percent_Identity=27.0742358078603, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=46.4028776978417, Blast_Score=253, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=38.3050847457627, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=44.5614035087719, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321643, Length=297, Percent_Identity=39.3939393939394, Blast_Score=213, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=312, Percent_Identity=25.9615384615385, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17136818, Length=292, Percent_Identity=46.9178082191781, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17136816, Length=292, Percent_Identity=46.9178082191781, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=42.1602787456446, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=42.1602787456446, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=42.1602787456446, Blast_Score=220, Evalue=7e-58,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=42.1602787456446, Blast_Score=220, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_NITEC (Q0AFN2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747815.1
- ProteinModelPortal:   Q0AFN2
- SMR:   Q0AFN2
- STRING:   Q0AFN2
- GeneID:   4272095
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1607
- NMPDR:   fig|335283.3.peg.1909
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   LLEHYHI
- PhylomeDB:   Q0AFN2
- ProtClustDB:   PRK14189
- BioCyc:   NEUT335283:NEUT_1607-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31534; Mature: 31403

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATIISGNFIASKLREELKQRIRILSETWMQPGLAVILAGDNPASSVYVRNKARTCGEL
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHHH
GIHSKVFNFSADTPQKIILRQIQDLNANPEIHGILVQLPLPSHIQMNEVIAAIAIEKDVD
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHEEEEEECCCC
GFHPCNVGALATGHALFRPCTPFGVMKMLAEYGIPLQGQHAVVVGRSNIVGKPMALMLLE
CCCCCCCCCEECCCHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEC
QGATVTICTSQTRELASHTRSADIIVMATGKANLLTSDMIRTGATVIDVGINRLADGQLC
CCCEEEEECCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCEEEEECHHHCCCCCEE
GDVEFSGVKEKAGYITPVPGGVGPMTITMLMNNTIEAAEHAKAKAQIEARCGSMQ
CCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SATIISGNFIASKLREELKQRIRILSETWMQPGLAVILAGDNPASSVYVRNKARTCGEL
CEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCHHHHH
GIHSKVFNFSADTPQKIILRQIQDLNANPEIHGILVQLPLPSHIQMNEVIAAIAIEKDVD
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHEEEEEECCCC
GFHPCNVGALATGHALFRPCTPFGVMKMLAEYGIPLQGQHAVVVGRSNIVGKPMALMLLE
CCCCCCCCCEECCCHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEC
QGATVTICTSQTRELASHTRSADIIVMATGKANLLTSDMIRTGATVIDVGINRLADGQLC
CCCEEEEECCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCEEEEECHHHCCCCCEE
GDVEFSGVKEKAGYITPVPGGVGPMTITMLMNNTIEAAEHAKAKAQIEARCGSMQ
CCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA