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The map label for this gene is glgB

Identifier: 114331285

GI number: 114331285

Start: 1352413

End: 1354611

Strand: Reverse

Name: glgB

Synonym: Neut_1291

Alternate gene names: 114331285

Gene position: 1354611-1352413 (Counterclockwise)

Preceding gene: 114331290

Following gene: 114331284

Centisome position: 50.9

GC content: 48.66

Gene sequence:

>2199_bases
ATGAAATCTTTGCCTTTCCCATCCCCATCCCGAGATTTAGCAGGTGATTCTGCGCAATCATTGTTATTGGCCGCAAGGCT
GCATGCCCCCGGCTCTTATCTGGGGATACATTCTGTTCCGGGCGGTGAGCTGGTTCGAGTGTTTCAACCGTATATGTCAC
GGGTATGGCTGCAGACTTCATCCGGTTTCCAGGTGATGGAGTGCACGCACGATGCAGGTATTTTCGAGTGGAAGGGGGAG
GCTGTTACGCGTCCTTATTTACTGCGTCTGGAACATGCAGAAACCGGGGTAATTGAGGAGCGCTATGATCCTTATGCTTT
TCCAGTACAGATTTCAGGACATGATCTGTATCTGTTCAATGAAGGGCGCCTGCTCCAGGCATACCACATGCTGGGTACCC
ATCAGGTCAAAAATCAAGGCGTTACGGGCACACGTTTTGCCGTATGGGCACCCAATGCCGAACGGGTAAGTGTCGTTGGG
GATTTCAATCACTGGGATGGCAGGGTGTGCCCTATGATGGCGCGTGATCACAGCGGGGTATGGGAATTGTTTATTCCGGA
TCTGCCCGGAGGTACGTTATATAAATATGAGATCAGAAATCATTCAACTGGAGAAATCCTGCTCAAGACCGATCCTTATG
CAACCAGGTATGAATTGCGTCCCAATAATGCCGCATTGACGCCAGTTGAAAGCCGGTATGAATGGCAGGATAACGACTGG
ATGGTGCAACGCGCAGGATGGGACTGGCTACATGCGCCAGTCAATATTTATGAGCTGCATGTAGGTTCGTGGAAACGCCA
TCCGGATGGCAGCTTCTATTCATATCAGGAATTAGCCGATCACCTGATTCCTTATCTACAAGAGATGGGTTATTCCCATG
TTGAGCTGTTGCCAATTTCCGAGCACCCGCTGGATGAATCCTGGGGATATCAGGTAACAGGTTATTTTGCAGCAACCAGC
CGCTATGGTAATCCAGAAGCATTCATGTATTTTGTGGATAAATGTCATCAGGCTGGAATTGGTGTGATTCTCGACTGGGT
GCCGGCACATTTTCCACAAGACAGTTTTTCATTGGCACGGTTTGACGGTACAGCGCTCTATGAACATGAAGATCCGCGCC
TGGGTTATCACCAGGATTGGGGGACGTATATTTTCAATTACGGGCGCAGTGAGGTGAAATCCTTTCTGCTTTCCAGCGCC
CATTACTGGTTGTCTGTTTTCCATATTGACGGGCTGCGTGTGGATGCGGTGGCCTCGATGCTCTATCTGGATTATTCGCG
CAAAGAAGGAGAATGGCTGCCCAATCGTTTTGGTGGGCGTGAAAATCTTGATGCAATTGATTTCTTGCGTGAATTGAACA
CGATGGTGCATGGTGAATTTTCCGGTGCGCTTACTTTTGCCGAGGAATCCACCTCCTGGCCAGCAGTATCTCGTCCGACC
TATCTGGGCGGGCTGGGTTTTTCCATGAAATGGAACATGGGATGGATGAACGATACGCTGAATTACATGCAACTGGATCC
AATTCACCGGCATTACCATCACAATGAACTAACGTTCAATCAGCTTTACGCCTATACCGAAAACTTCATTTTGCCGCTTT
CGCATGATGAAGTGGTACATGGCAAAAAATCCCTGCTGGACAAGATGTCGGGAGATGTCTGGCAAATGTTTGCCAACCTG
CGTTTGCTGTTTACCTACCAGATGACCTGCCCAGGCAAAAAAATCAACTTTATGGGCAATGAATTTGCTCAAGGACGTGA
ATGGCGGGTTAATCATGAACTTGACTGGTATCTGCTGGAGCGTGATCCGCATCGTGGTATACAAATGTTATTGCGGAATC
TGAATCACCTCTACCTGGATACACCAGCGTTGCATGAGCTGGATTTTTTTACTGAAGGATTTTCCTGGATTGATTGTCAT
GATTCGGAACAGTCAGTTATCAGTTACCAGCGCCGTGCCAGAGATGGATCATTCATGCTGGTGATCCTGAATTTCACACC
AGTGCCACGTACCGGCTACCGAGTAGGCGTACCGGAATCCCGAACATACCAAGAAGTATTCAACAGCGATTCAACTTACT
ATGGTGGCAGCAATATCGGTAACCCTGGGGATATTGTTCCGACCGGGCAGCAGTGGTCAGGTCAAGTTGATTCCATCATC
ATTACCTTACCGCCGCTGGCGGGTATCATTCTGATTTGA

Upstream 100 bases:

>100_bases
GTGTTTAAAAAAACCGCATACCCTGTGTTTCCATGTTTATCTTACTTGATCCGAATAACCATTACCAGCCGACACTGCAG
GTAATAGCGAGCCCATACCG

Downstream 100 bases:

>100_bases
CAGCAGATTGTGCTTGTAAAAGCAGGAATTTATTCAGATTAAGGTTTGTCTTCATATAAGAGTGGCAAAAGAGCCAGTCT
GTATATCGAAAATTGATTTG

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MKSLPFPSPSRDLAGDSAQSLLLAARLHAPGSYLGIHSVPGGELVRVFQPYMSRVWLQTSSGFQVMECTHDAGIFEWKGE
AVTRPYLLRLEHAETGVIEERYDPYAFPVQISGHDLYLFNEGRLLQAYHMLGTHQVKNQGVTGTRFAVWAPNAERVSVVG
DFNHWDGRVCPMMARDHSGVWELFIPDLPGGTLYKYEIRNHSTGEILLKTDPYATRYELRPNNAALTPVESRYEWQDNDW
MVQRAGWDWLHAPVNIYELHVGSWKRHPDGSFYSYQELADHLIPYLQEMGYSHVELLPISEHPLDESWGYQVTGYFAATS
RYGNPEAFMYFVDKCHQAGIGVILDWVPAHFPQDSFSLARFDGTALYEHEDPRLGYHQDWGTYIFNYGRSEVKSFLLSSA
HYWLSVFHIDGLRVDAVASMLYLDYSRKEGEWLPNRFGGRENLDAIDFLRELNTMVHGEFSGALTFAEESTSWPAVSRPT
YLGGLGFSMKWNMGWMNDTLNYMQLDPIHRHYHHNELTFNQLYAYTENFILPLSHDEVVHGKKSLLDKMSGDVWQMFANL
RLLFTYQMTCPGKKINFMGNEFAQGREWRVNHELDWYLLERDPHRGIQMLLRNLNHLYLDTPALHELDFFTEGFSWIDCH
DSEQSVISYQRRARDGSFMLVILNFTPVPRTGYRVGVPESRTYQEVFNSDSTYYGGSNIGNPGDIVPTGQQWSGQVDSII
ITLPPLAGIILI

Sequences:

>Translated_732_residues
MKSLPFPSPSRDLAGDSAQSLLLAARLHAPGSYLGIHSVPGGELVRVFQPYMSRVWLQTSSGFQVMECTHDAGIFEWKGE
AVTRPYLLRLEHAETGVIEERYDPYAFPVQISGHDLYLFNEGRLLQAYHMLGTHQVKNQGVTGTRFAVWAPNAERVSVVG
DFNHWDGRVCPMMARDHSGVWELFIPDLPGGTLYKYEIRNHSTGEILLKTDPYATRYELRPNNAALTPVESRYEWQDNDW
MVQRAGWDWLHAPVNIYELHVGSWKRHPDGSFYSYQELADHLIPYLQEMGYSHVELLPISEHPLDESWGYQVTGYFAATS
RYGNPEAFMYFVDKCHQAGIGVILDWVPAHFPQDSFSLARFDGTALYEHEDPRLGYHQDWGTYIFNYGRSEVKSFLLSSA
HYWLSVFHIDGLRVDAVASMLYLDYSRKEGEWLPNRFGGRENLDAIDFLRELNTMVHGEFSGALTFAEESTSWPAVSRPT
YLGGLGFSMKWNMGWMNDTLNYMQLDPIHRHYHHNELTFNQLYAYTENFILPLSHDEVVHGKKSLLDKMSGDVWQMFANL
RLLFTYQMTCPGKKINFMGNEFAQGREWRVNHELDWYLLERDPHRGIQMLLRNLNHLYLDTPALHELDFFTEGFSWIDCH
DSEQSVISYQRRARDGSFMLVILNFTPVPRTGYRVGVPESRTYQEVFNSDSTYYGGSNIGNPGDIVPTGQQWSGQVDSII
ITLPPLAGIILI
>Mature_732_residues
MKSLPFPSPSRDLAGDSAQSLLLAARLHAPGSYLGIHSVPGGELVRVFQPYMSRVWLQTSSGFQVMECTHDAGIFEWKGE
AVTRPYLLRLEHAETGVIEERYDPYAFPVQISGHDLYLFNEGRLLQAYHMLGTHQVKNQGVTGTRFAVWAPNAERVSVVG
DFNHWDGRVCPMMARDHSGVWELFIPDLPGGTLYKYEIRNHSTGEILLKTDPYATRYELRPNNAALTPVESRYEWQDNDW
MVQRAGWDWLHAPVNIYELHVGSWKRHPDGSFYSYQELADHLIPYLQEMGYSHVELLPISEHPLDESWGYQVTGYFAATS
RYGNPEAFMYFVDKCHQAGIGVILDWVPAHFPQDSFSLARFDGTALYEHEDPRLGYHQDWGTYIFNYGRSEVKSFLLSSA
HYWLSVFHIDGLRVDAVASMLYLDYSRKEGEWLPNRFGGRENLDAIDFLRELNTMVHGEFSGALTFAEESTSWPAVSRPT
YLGGLGFSMKWNMGWMNDTLNYMQLDPIHRHYHHNELTFNQLYAYTENFILPLSHDEVVHGKKSLLDKMSGDVWQMFANL
RLLFTYQMTCPGKKINFMGNEFAQGREWRVNHELDWYLLERDPHRGIQMLLRNLNHLYLDTPALHELDFFTEGFSWIDCH
DSEQSVISYQRRARDGSFMLVILNFTPVPRTGYRVGVPESRTYQEVFNSDSTYYGGSNIGNPGDIVPTGQQWSGQVDSII
ITLPPLAGIILI

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=678, Percent_Identity=29.3510324483776, Blast_Score=209, Evalue=6e-54,
Organism=Escherichia coli, GI1789839, Length=722, Percent_Identity=52.0775623268698, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI2367229, Length=334, Percent_Identity=28.1437125748503, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554896, Length=644, Percent_Identity=27.1739130434783, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI32564391, Length=608, Percent_Identity=26.6447368421053, Blast_Score=172, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6320826, Length=678, Percent_Identity=26.401179941003, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI28573410, Length=654, Percent_Identity=29.3577981651376, Blast_Score=205, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_NITEC (Q0AGJ0)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747507.1
- ProteinModelPortal:   Q0AGJ0
- STRING:   Q0AGJ0
- GeneID:   4272035
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1291
- NMPDR:   fig|335283.3.peg.1349
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- PhylomeDB:   Q0AGJ0
- ProtClustDB:   PRK05402
- BioCyc:   NEUT335283:NEUT_1291-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 84191; Mature: 84191

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: ACT_SITE 310-310 ACT_SITE 345-345 ACT_SITE 350-350 ACT_SITE 413-413 ACT_SITE 415-415 ACT_SITE 468-468 ACT_SITE 535-535 ACT_SITE 536-536

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLPFPSPSRDLAGDSAQSLLLAARLHAPGSYLGIHSVPGGELVRVFQPYMSRVWLQTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHEEEECC
SGFQVMECTHDAGIFEWKGEAVTRPYLLRLEHAETGVIEERYDPYAFPVQISGHDLYLFN
CCCEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCEEEEEC
EGRLLQAYHMLGTHQVKNQGVTGTRFAVWAPNAERVSVVGDFNHWDGRVCPMMARDHSGV
CCCEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCEECCEEECCCCCE
WELFIPDLPGGTLYKYEIRNHSTGEILLKTDPYATRYELRPNNAALTPVESRYEWQDNDW
EEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCCEECCCCCCCEECCCCE
MVQRAGWDWLHAPVNIYELHVGSWKRHPDGSFYSYQELADHLIPYLQEMGYSHVELLPIS
EEEECCCCEEECCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEECC
EHPLDESWGYQVTGYFAATSRYGNPEAFMYFVDKCHQAGIGVILDWVPAHFPQDSFSLAR
CCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEE
FDGTALYEHEDPRLGYHQDWGTYIFNYGRSEVKSFLLSSAHYWLSVFHIDGLRVDAVASM
ECCEEEEECCCCCCCCCCCCCHHEECCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
LYLDYSRKEGEWLPNRFGGRENLDAIDFLRELNTMVHGEFSGALTFAEESTSWPAVSRPT
HHEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
YLGGLGFSMKWNMGWMNDTLNYMQLDPIHRHYHHNELTFNQLYAYTENFILPLSHDEVVH
EEECCCEEEEECCCCCCCCCCCEEECHHHHHHCCCCCCHHHHHHHHCCEEEECCCCHHHC
GKKSLLDKMSGDVWQMFANLRLLFTYQMTCPGKKINFMGNEFAQGREWRVNHELDWYLLE
CHHHHHHHHCCHHHHHHHCEEEEEEEEEECCCCEEECCCCHHCCCCCEEECCCEEEEEEE
RDPHRGIQMLLRNLNHLYLDTPALHELDFFTEGFSWIDCHDSEQSVISYQRRARDGSFML
CCCCHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEE
VILNFTPVPRTGYRVGVPESRTYQEVFNSDSTYYGGSNIGNPGDIVPTGQQWSGQVDSII
EEEECCCCCCCCEEECCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEE
ITLPPLAGIILI
EEECCCCCEEEC
>Mature Secondary Structure
MKSLPFPSPSRDLAGDSAQSLLLAARLHAPGSYLGIHSVPGGELVRVFQPYMSRVWLQTS
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHEEEECC
SGFQVMECTHDAGIFEWKGEAVTRPYLLRLEHAETGVIEERYDPYAFPVQISGHDLYLFN
CCCEEEEEECCCCEEEECCCCCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCEEEEEC
EGRLLQAYHMLGTHQVKNQGVTGTRFAVWAPNAERVSVVGDFNHWDGRVCPMMARDHSGV
CCCEEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCCEECCEEECCCCCE
WELFIPDLPGGTLYKYEIRNHSTGEILLKTDPYATRYELRPNNAALTPVESRYEWQDNDW
EEEEECCCCCCCEEEEEECCCCCCEEEEECCCCCEEEEECCCCCEECCCCCCCEECCCCE
MVQRAGWDWLHAPVNIYELHVGSWKRHPDGSFYSYQELADHLIPYLQEMGYSHVELLPIS
EEEECCCCEEECCCEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEEECC
EHPLDESWGYQVTGYFAATSRYGNPEAFMYFVDKCHQAGIGVILDWVPAHFPQDSFSLAR
CCCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEE
FDGTALYEHEDPRLGYHQDWGTYIFNYGRSEVKSFLLSSAHYWLSVFHIDGLRVDAVASM
ECCEEEEECCCCCCCCCCCCCHHEECCCHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
LYLDYSRKEGEWLPNRFGGRENLDAIDFLRELNTMVHGEFSGALTFAEESTSWPAVSRPT
HHEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCC
YLGGLGFSMKWNMGWMNDTLNYMQLDPIHRHYHHNELTFNQLYAYTENFILPLSHDEVVH
EEECCCEEEEECCCCCCCCCCCEEECHHHHHHCCCCCCHHHHHHHHCCEEEECCCCHHHC
GKKSLLDKMSGDVWQMFANLRLLFTYQMTCPGKKINFMGNEFAQGREWRVNHELDWYLLE
CHHHHHHHHCCHHHHHHHCEEEEEEEEEECCCCEEECCCCHHCCCCCEEECCCEEEEEEE
RDPHRGIQMLLRNLNHLYLDTPALHELDFFTEGFSWIDCHDSEQSVISYQRRARDGSFML
CCCCHHHHHHHHHCCEEEECCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCCEEE
VILNFTPVPRTGYRVGVPESRTYQEVFNSDSTYYGGSNIGNPGDIVPTGQQWSGQVDSII
EEEECCCCCCCCEEECCCCCHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCEEE
ITLPPLAGIILI
EEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA