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The map label for this gene is rimO

Identifier: 113477201

GI number: 113477201

Start: 5730128

End: 5731453

Strand: Reverse

Name: rimO

Synonym: Tery_3733

Alternate gene names: 113477201

Gene position: 5731453-5730128 (Counterclockwise)

Preceding gene: 113477204

Following gene: 113477200

Centisome position: 73.95

GC content: 40.42

Gene sequence:

>1326_bases
ATGTCGACAAAACCTACTATTGCATTTACTCACCTGGGATGCGAAAAAAACCGCATTGATACTGAACATATCATTGGTTT
ACTTGTACAGGCAGGTTATGAAGTAGATGCTAACGAAGAATTAGCAGATTATGTTGTTGTCAATACTTGTAGTTTTATCC
AAGCTGCTAGAGAAGAGTCTGTCAAAACTTTAGTAGAATTAGCTGAAGCTAATAAAAAAATAGTCATCGCAGGTTGTATG
GCACAACACTTCCCTGAAGAATTACTAGCTGAGTTGCCAGAAGCAATAGCACTGGTGGGGACTGGTGATTATCATAAAAT
AGTAGATGTGATGCAACGGGTAGAAAAAGGGGATCGTGTCAAAGAAGTTACTGCAGAACCTACTTACATCGCTGACGAAA
CTACCCCCCGGTACCGCACAACATCTGAAGGTGTTGCTTATGTGCGGATCGCCGAAGGATGTGATTATCGTTGCGCTTTC
TGTATTATTCCTCATCTCCGGGGGAAAGCACGATCACGCACCATAGAATCTATTGTCGTCGAAGCTCAAAAACTAGCGGA
CCAGGGTGTGAAAGAAATCATCCTCATTTCCCAAATAACAACTAATTATGGAATTGATATTTACGGTCAACCTAAATTGG
CAGATCTGCTTGAGGCCCTCGGAAAAGTTGATATACCCTGGATTAGAATGCACTATGCTTATCCCACTGGTTTGACACCC
AAGGTTATAACTGCCATCCAAGATACTCCCAATATTTTACCTTATCTTGATTTACCTCTACAACATTCTCATCCCGAAAT
TCTCAGGGCTATGAATAGACCTTGGCAAGGTCAGGTTAATGATGATATAATTAAAAGAATAAAAACAGCTATGCCTAATG
CTGTATTACGGACAAGTTTTATAGTAGGTTTTCCTGGTGAAACTGAAGAACATCACTCACACTTAGTGGAATTTGTCAAG
CGTCATGAGTTTGATCATGTCGGAGTCTTTACTTTTTCTCCAGAAGAGGGAACTCCTGCTTATAATTTGCCCAATCAGTT
GCCCCAAGAAGTTATGGACGCTCGTCGTCAAGAAATTATGGAAGTTCAGCAGTCCATATCTTGGCAACAGAATCAAAAAT
TAGTGGGGCAATTAGTTGATGTATTGATTGAACAAGAAAATCCTCAAACAGGAGAGTTAATTGGTCGATCCCCACGGTTT
TCTCCAGAAGTTGATGGACTTATTTATGTGAAAGGGGAAGCTAGGTTAGGATGTATTGTTCCAGTGATGATTACAGATGC
TGATATTTACGATTTGTATGGTTGTTTGATAAGCTCTGCGGCCTAA

Upstream 100 bases:

>100_bases
TAACTTAATTGAATGCTTTACTTCAGGATAAAATTCAGTACTTTGATGTGATAAATGTGCTATATTAAAAAACTGTTGCT
AATAGAGATATATAACGGAA

Downstream 100 bases:

>100_bases
AGGCGGAGCCCAGAGATTTTGCTGAAACTTTAGATTATTTATTAATTGACTGGGATTATTTATTCATTGAGTCCACTGAG
ATTAAAGACCTTGATATATC

Product: MiaB-like tRNA modifying protein YliG

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSTKPTIAFTHLGCEKNRIDTEHIIGLLVQAGYEVDANEELADYVVVNTCSFIQAAREESVKTLVELAEANKKIVIAGCM
AQHFPEELLAELPEAIALVGTGDYHKIVDVMQRVEKGDRVKEVTAEPTYIADETTPRYRTTSEGVAYVRIAEGCDYRCAF
CIIPHLRGKARSRTIESIVVEAQKLADQGVKEIILISQITTNYGIDIYGQPKLADLLEALGKVDIPWIRMHYAYPTGLTP
KVITAIQDTPNILPYLDLPLQHSHPEILRAMNRPWQGQVNDDIIKRIKTAMPNAVLRTSFIVGFPGETEEHHSHLVEFVK
RHEFDHVGVFTFSPEEGTPAYNLPNQLPQEVMDARRQEIMEVQQSISWQQNQKLVGQLVDVLIEQENPQTGELIGRSPRF
SPEVDGLIYVKGEARLGCIVPVMITDADIYDLYGCLISSAA

Sequences:

>Translated_441_residues
MSTKPTIAFTHLGCEKNRIDTEHIIGLLVQAGYEVDANEELADYVVVNTCSFIQAAREESVKTLVELAEANKKIVIAGCM
AQHFPEELLAELPEAIALVGTGDYHKIVDVMQRVEKGDRVKEVTAEPTYIADETTPRYRTTSEGVAYVRIAEGCDYRCAF
CIIPHLRGKARSRTIESIVVEAQKLADQGVKEIILISQITTNYGIDIYGQPKLADLLEALGKVDIPWIRMHYAYPTGLTP
KVITAIQDTPNILPYLDLPLQHSHPEILRAMNRPWQGQVNDDIIKRIKTAMPNAVLRTSFIVGFPGETEEHHSHLVEFVK
RHEFDHVGVFTFSPEEGTPAYNLPNQLPQEVMDARRQEIMEVQQSISWQQNQKLVGQLVDVLIEQENPQTGELIGRSPRF
SPEVDGLIYVKGEARLGCIVPVMITDADIYDLYGCLISSAA
>Mature_440_residues
STKPTIAFTHLGCEKNRIDTEHIIGLLVQAGYEVDANEELADYVVVNTCSFIQAAREESVKTLVELAEANKKIVIAGCMA
QHFPEELLAELPEAIALVGTGDYHKIVDVMQRVEKGDRVKEVTAEPTYIADETTPRYRTTSEGVAYVRIAEGCDYRCAFC
IIPHLRGKARSRTIESIVVEAQKLADQGVKEIILISQITTNYGIDIYGQPKLADLLEALGKVDIPWIRMHYAYPTGLTPK
VITAIQDTPNILPYLDLPLQHSHPEILRAMNRPWQGQVNDDIIKRIKTAMPNAVLRTSFIVGFPGETEEHHSHLVEFVKR
HEFDHVGVFTFSPEEGTPAYNLPNQLPQEVMDARRQEIMEVQQSISWQQNQKLVGQLVDVLIEQENPQTGELIGRSPRFS
PEVDGLIYVKGEARLGCIVPVMITDADIYDLYGCLISSAA

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=422, Percent_Identity=28.6729857819905, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI28872782, Length=422, Percent_Identity=28.6729857819905, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI93277076, Length=438, Percent_Identity=26.2557077625571, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787057, Length=453, Percent_Identity=38.8520971302428, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1786882, Length=457, Percent_Identity=30.1969365426696, Blast_Score=174, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=479, Percent_Identity=27.5574112734864, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71996771, Length=318, Percent_Identity=26.7295597484277, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21356207, Length=452, Percent_Identity=27.212389380531, Blast_Score=165, Evalue=6e-41,
Organism=Drosophila melanogaster, GI19922432, Length=441, Percent_Identity=25.3968253968254, Blast_Score=131, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_TRIEI (Q10Y85)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_723262.1
- ProteinModelPortal:   Q10Y85
- SMR:   Q10Y85
- STRING:   Q10Y85
- GeneID:   4243909
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_3733
- NMPDR:   fig|203124.1.peg.6008
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q10Y85
- ProtClustDB:   CLSK893584
- BioCyc:   TERY203124:TERY_3733-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49330; Mature: 49199

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPTIAFTHLGCEKNRIDTEHIIGLLVQAGYEVDANEELADYVVVNTCSFIQAAREES
CCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKTLVELAEANKKIVIAGCMAQHFPEELLAELPEAIALVGTGDYHKIVDVMQRVEKGDRV
HHHHHHHHHCCCEEEEEEHHHHHCHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHCCCCC
KEVTAEPTYIADETTPRYRTTSEGVAYVRIAEGCDYRCAFCIIPHLRGKARSRTIESIVV
CEECCCCCEEECCCCCCCEECCCCEEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHHHH
EAQKLADQGVKEIILISQITTNYGIDIYGQPKLADLLEALGKVDIPWIRMHYAYPTGLTP
HHHHHHHCCHHHEEHHEEHHHCCCEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCH
KVITAIQDTPNILPYLDLPLQHSHPEILRAMNRPWQGQVNDDIIKRIKTAMPNAVLRTSF
HHHHHHHCCCCCCCEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHEE
IVGFPGETEEHHSHLVEFVKRHEFDHVGVFTFSPEEGTPAYNLPNQLPQEVMDARRQEIM
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVQQSISWQQNQKLVGQLVDVLIEQENPQTGELIGRSPRFSPEVDGLIYVKGEARLGCIV
HHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEE
PVMITDADIYDLYGCLISSAA
EEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
STKPTIAFTHLGCEKNRIDTEHIIGLLVQAGYEVDANEELADYVVVNTCSFIQAAREES
CCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKTLVELAEANKKIVIAGCMAQHFPEELLAELPEAIALVGTGDYHKIVDVMQRVEKGDRV
HHHHHHHHHCCCEEEEEEHHHHHCHHHHHHHCHHHEEEECCCCHHHHHHHHHHHHCCCCC
KEVTAEPTYIADETTPRYRTTSEGVAYVRIAEGCDYRCAFCIIPHLRGKARSRTIESIVV
CEECCCCCEEECCCCCCCEECCCCEEEEEEECCCCCEEEEEEEHHHCCCHHHHHHHHHHH
EAQKLADQGVKEIILISQITTNYGIDIYGQPKLADLLEALGKVDIPWIRMHYAYPTGLTP
HHHHHHHCCHHHEEHHEEHHHCCCEEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCCCH
KVITAIQDTPNILPYLDLPLQHSHPEILRAMNRPWQGQVNDDIIKRIKTAMPNAVLRTSF
HHHHHHHCCCCCCCEECCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHEE
IVGFPGETEEHHSHLVEFVKRHEFDHVGVFTFSPEEGTPAYNLPNQLPQEVMDARRQEIM
EEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
EVQQSISWQQNQKLVGQLVDVLIEQENPQTGELIGRSPRFSPEVDGLIYVKGEARLGCIV
HHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCEEEE
PVMITDADIYDLYGCLISSAA
EEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA