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The map label for this gene is mcrB [H]

Identifier: 113477076

GI number: 113477076

Start: 5510931

End: 5512550

Strand: Reverse

Name: mcrB [H]

Synonym: Tery_3583

Alternate gene names: 113477076

Gene position: 5512550-5510931 (Counterclockwise)

Preceding gene: 113477077

Following gene: 113477075

Centisome position: 71.13

GC content: 35.0

Gene sequence:

>1620_bases
ATGGCAATTCAACAACTAACTTTATTTGTTCAACTTTTTCAAGAATTTTCTGACTCATATCCCTATAGCTTAGAAGGATT
AAATCATAGAGCATCTTATGAAAAACAACGTCAACAAGGTCGCCGGAATTTTGAAGAAATAACTACTGCTGCTAAGGCAG
GGGAAGACATAACAGAGTTGATTATATGGAAACTTTTACCTCATACTAATACTCCCAGTAATCGACAAAAGGGTGTATGG
ATTTACTTGAGTGAATCTAGCAATACTGATTTAAGTGAATGGTTTCAATGGTTGGAAAAGCAAGAAGATTGGTCTGTTAT
TACTAGAGCAATTTATAATTTTGTAACTTCCTGTATTACAGATTATCATAAATTTTTAGTAGCTTGTAAAAATTTTTCTA
AATTACCATATTCTAGAGAATTTAAAATAGGAATAATAACACCTATTCTGAATGCAGTCAAACCAGATGATTTTCTTTTA
ATTAATAATAAATCCAGAAAAGTAATTAATTATTTAAGTAACACAAAACATACTCAAAAGCTAACTGAATACCCATATAT
TAATCTCACTGGCCATAATCTAATTTTAGAATTAGCTGAAGAAATGCAGAAGCAAAATATACCTGCAATGCGAGATGATG
ATTTGTTTGATATGTTTTGTTATTGGTTGGTAGATGTAAAAAAATACAATTTTATTAGTGAAAATATAAAGAGTACAGAC
AATATCTATAAAACTAATCAAGATCAAGATCAAAAAATAAATGTGATTAATTTACAACCAGAATACACTCTAACTCAATG
CTCCCAAGAAACAAACTTCACAGAAACAGAACTCACCCAATGGATACGCGCTATCAACCGTAAAAAACAGGCTATTTTCC
AAGGTCCACCTGGTACAGGCAAAACTTTCCTCTCTCAAAAACTCGCCCAACACTTAATCAGTCGAGGTGACGGCTTCTGG
GAACTGATCCAATTTCATTCTGCTTATACCTACGAAGACTTTATTCAAGGTATTCGACCCCAAAGCCAAGATGGTAAACT
CACCTACCCTGTTGTTCCCGGTCGCTTCCTACAATTTTGTCAAAAAGCAGCACTTAATCAAGATATTTGCGTACTCATCA
TTGATGAAATTAACCGGGCTAACCTCTCCCAAGTTTTCGGCGAACTTATGTACCTCCTGGAATACCGCGACGCCAAAATT
CCCCTTGCTGGTAGTGCAGAATTATTCAGCATTCCTAAAAATGTTCGCATCATTGGCACTATGAATACTGCCGATCGCTC
CATTGCTTTAGTAGACCATGCACTTCGTCGTCGCTTTGCTTTCATCACTCTCTCCCCTAAGTATGAAATATTGCTTCAAT
ACTACAAGGAAATAGAAACAAATTTTTCAGTGGAGGGGTTAACTCAACTTTTAGAAGCAGTCAACCAAGAAATAAACGAC
CCCAACTATCAAGTCGGTGTTTCCTTCTTTCTCCGAAAAAATATTGCAGAGGAAATTCCAGATATTTGGCAAATGGAAAT
TGAACCATATTTAGAGGAATATTTCTTTGCTCAACCAGAAAAAGTCAATGATTTTCGGTGGGAAAAAATTCAACATCGGA
TGTCAAAGACAATTTGTTAG

Upstream 100 bases:

>100_bases
TAGCCCAATTAAAAATTATTTTCAGTTAAGAGTTAGGCCATCGCAATTCTTGCACAATCATAGTCAAAATAACCAACACC
AAAAATTAAAATAACTGTGT

Downstream 100 bases:

>100_bases
ATATAGACTTTTCCCCACATCTCAGATGCAAAAAGTATCTATTATGTCTGCTTCAAAAACAATATGAAATCTTCCCAAAT
CATCGAACTCACTGAATATC

Product: ATPase

Products: NA

Alternate protein names: EcoKMcrBC [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MAIQQLTLFVQLFQEFSDSYPYSLEGLNHRASYEKQRQQGRRNFEEITTAAKAGEDITELIIWKLLPHTNTPSNRQKGVW
IYLSESSNTDLSEWFQWLEKQEDWSVITRAIYNFVTSCITDYHKFLVACKNFSKLPYSREFKIGIITPILNAVKPDDFLL
INNKSRKVINYLSNTKHTQKLTEYPYINLTGHNLILELAEEMQKQNIPAMRDDDLFDMFCYWLVDVKKYNFISENIKSTD
NIYKTNQDQDQKINVINLQPEYTLTQCSQETNFTETELTQWIRAINRKKQAIFQGPPGTGKTFLSQKLAQHLISRGDGFW
ELIQFHSAYTYEDFIQGIRPQSQDGKLTYPVVPGRFLQFCQKAALNQDICVLIIDEINRANLSQVFGELMYLLEYRDAKI
PLAGSAELFSIPKNVRIIGTMNTADRSIALVDHALRRRFAFITLSPKYEILLQYYKEIETNFSVEGLTQLLEAVNQEIND
PNYQVGVSFFLRKNIAEEIPDIWQMEIEPYLEEYFFAQPEKVNDFRWEKIQHRMSKTIC

Sequences:

>Translated_539_residues
MAIQQLTLFVQLFQEFSDSYPYSLEGLNHRASYEKQRQQGRRNFEEITTAAKAGEDITELIIWKLLPHTNTPSNRQKGVW
IYLSESSNTDLSEWFQWLEKQEDWSVITRAIYNFVTSCITDYHKFLVACKNFSKLPYSREFKIGIITPILNAVKPDDFLL
INNKSRKVINYLSNTKHTQKLTEYPYINLTGHNLILELAEEMQKQNIPAMRDDDLFDMFCYWLVDVKKYNFISENIKSTD
NIYKTNQDQDQKINVINLQPEYTLTQCSQETNFTETELTQWIRAINRKKQAIFQGPPGTGKTFLSQKLAQHLISRGDGFW
ELIQFHSAYTYEDFIQGIRPQSQDGKLTYPVVPGRFLQFCQKAALNQDICVLIIDEINRANLSQVFGELMYLLEYRDAKI
PLAGSAELFSIPKNVRIIGTMNTADRSIALVDHALRRRFAFITLSPKYEILLQYYKEIETNFSVEGLTQLLEAVNQEIND
PNYQVGVSFFLRKNIAEEIPDIWQMEIEPYLEEYFFAQPEKVNDFRWEKIQHRMSKTIC
>Mature_538_residues
AIQQLTLFVQLFQEFSDSYPYSLEGLNHRASYEKQRQQGRRNFEEITTAAKAGEDITELIIWKLLPHTNTPSNRQKGVWI
YLSESSNTDLSEWFQWLEKQEDWSVITRAIYNFVTSCITDYHKFLVACKNFSKLPYSREFKIGIITPILNAVKPDDFLLI
NNKSRKVINYLSNTKHTQKLTEYPYINLTGHNLILELAEEMQKQNIPAMRDDDLFDMFCYWLVDVKKYNFISENIKSTDN
IYKTNQDQDQKINVINLQPEYTLTQCSQETNFTETELTQWIRAINRKKQAIFQGPPGTGKTFLSQKLAQHLISRGDGFWE
LIQFHSAYTYEDFIQGIRPQSQDGKLTYPVVPGRFLQFCQKAALNQDICVLIIDEINRANLSQVFGELMYLLEYRDAKIP
LAGSAELFSIPKNVRIIGTMNTADRSIALVDHALRRRFAFITLSPKYEILLQYYKEIETNFSVEGLTQLLEAVNQEINDP
NYQVGVSFFLRKNIAEEIPDIWQMEIEPYLEEYFFAQPEKVNDFRWEKIQHRMSKTIC

Specific function: Recognizes N4- and C5-methylcytosine (and 5-hydroxy- methylcytosines) produced by a broad range of DNA methylases and appears to act against 5-methylcytosine preceded by a purine residue. Binds to DNA containing methylated cytosines; also binds to GTP. Is

COG id: COG1401

COG function: function code V; GTPase subunit of restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082429, Length=287, Percent_Identity=40.0696864111498, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011704
- InterPro:   IPR021961
- InterPro:   IPR004491 [H]

Pfam domain/function: PF07728 AAA_5; PF12102 DUF3578 [H]

EC number: 3.1.21.- [C]

Molecular weight: Translated: 63187; Mature: 63055

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQQLTLFVQLFQEFSDSYPYSLEGLNHRASYEKQRQQGRRNFEEITTAAKAGEDITEL
CCHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IIWKLLPHTNTPSNRQKGVWIYLSESSNTDLSEWFQWLEKQEDWSVITRAIYNFVTSCIT
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DYHKFLVACKNFSKLPYSREFKIGIITPILNAVKPDDFLLINNKSRKVINYLSNTKHTQK
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHH
LTEYPYINLTGHNLILELAEEMQKQNIPAMRDDDLFDMFCYWLVDVKKYNFISENIKSTD
HHHCCEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NIYKTNQDQDQKINVINLQPEYTLTQCSQETNFTETELTQWIRAINRKKQAIFQGPPGTG
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCC
KTFLSQKLAQHLISRGDGFWELIQFHSAYTYEDFIQGIRPQSQDGKLTYPVVPGRFLQFC
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEECCCCHHHHHHH
QKAALNQDICVLIIDEINRANLSQVFGELMYLLEYRDAKIPLAGSAELFSIPKNVRIIGT
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCEEEEEE
MNTADRSIALVDHALRRRFAFITLSPKYEILLQYYKEIETNFSVEGLTQLLEAVNQEIND
CCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
PNYQVGVSFFLRKNIAEEIPDIWQMEIEPYLEEYFFAQPEKVNDFRWEKIQHRMSKTIC
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIQQLTLFVQLFQEFSDSYPYSLEGLNHRASYEKQRQQGRRNFEEITTAAKAGEDITEL
CHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
IIWKLLPHTNTPSNRQKGVWIYLSESSNTDLSEWFQWLEKQEDWSVITRAIYNFVTSCIT
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DYHKFLVACKNFSKLPYSREFKIGIITPILNAVKPDDFLLINNKSRKVINYLSNTKHTQK
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHH
LTEYPYINLTGHNLILELAEEMQKQNIPAMRDDDLFDMFCYWLVDVKKYNFISENIKSTD
HHHCCEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
NIYKTNQDQDQKINVINLQPEYTLTQCSQETNFTETELTQWIRAINRKKQAIFQGPPGTG
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCC
KTFLSQKLAQHLISRGDGFWELIQFHSAYTYEDFIQGIRPQSQDGKLTYPVVPGRFLQFC
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCCCCCCCCCEEECCCCHHHHHHH
QKAALNQDICVLIIDEINRANLSQVFGELMYLLEYRDAKIPLAGSAELFSIPKNVRIIGT
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEECCCCEEEEEE
MNTADRSIALVDHALRRRFAFITLSPKYEILLQYYKEIETNFSVEGLTQLLEAVNQEIND
CCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
PNYQVGVSFFLRKNIAEEIPDIWQMEIEPYLEEYFFAQPEKVNDFRWEKIQHRMSKTIC
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Endodeoxyribonucleases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203735; 2649480; 7610040; 9278503; 1312983; 7781618 [H]