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The map label for this gene is rihA [H]

Identifier: 113476968

GI number: 113476968

Start: 5305872

End: 5306795

Strand: Reverse

Name: rihA [H]

Synonym: Tery_3465

Alternate gene names: 113476968

Gene position: 5306795-5305872 (Counterclockwise)

Preceding gene: 113476972

Following gene: 113476967

Centisome position: 68.47

GC content: 35.71

Gene sequence:

>924_bases
ATGTCCAAAAAACTTGTACTAATGGATCATGACGGTGCAGTAGATGATTTTCTCTCCACTCTTTTACTCATGACTATGGA
AAATATTCAACCCTTAGGAGTAATTGTAACACCAGCTGACTGTTATATTGAACCCGCAATTAGTGCCACTCGAAAAATTT
TAGATTTAATAGGACGTTCTGATATTCCAGTAGCAGAAAGTACAGTCCGTGGAGTTAATCCTTTTCCGGTTTTATTTAGA
AGAGATTCTTTTGCAGTTGACCATTTTCCCATTTTAAATCAGAAGGAAAATATCAATACTATTTTAATTTCTGAACCTGG
TCAAAATTTTATGGTAAAAGTTTTAAAATCTGCACCAGCACCCGTAACATTAATGGTAACAGGTCCTTTAACAACTGTTG
CTACAGCATTAGATATTGCTCCAGAAATTGAGTCAAAAATTCAAGAAATTGTCTGGATGGGTGGTGCATTAAATGTACCA
GGAAATGTCAGTAAAGACATGGAACCAGGTCAAGATATGTCTGCTGAATGGAATGCCTATTGGGACCCGTTTGCTATTGA
AAGAGTTTGGCAAACTCAAATTCCTATTGTACTTTGCTCATTAGATATTACTAATAATGTTCCCCTAACTTCTGAATTTT
CTCACAAATTAAGTAAGCAAAGAAAGTATCCTATTTCTGATTTTGCCGGACAATGTTATGCATTAGCAATCTCTCAAGAC
TATTATTTTTGGGATGTTTTAGCAACTTCTTACTTAGCACATCCAGAATTTTATCAACTTCAAGAATGGGAAACTGTAGT
TATTACAAAAGGTCTCAGTCAAGGCCGGATAAAAGTAGAAAATGGTGGGCGGAAAATTATGGCAATGGAAACGGTCGATA
AAGAGAGATTTTACACTTATATTCTTCAACAATGGGCGCGGTAG

Upstream 100 bases:

>100_bases
AAGAAACAAGCAATAAATATTTACTCCCTCCTTTCTCCTCCCTCTTCTTTCTTCCTTAAAATCTTATATAATTTTGATTG
AAATCTAAACTTTAATATCT

Downstream 100 bases:

>100_bases
AACTAATTAATAGTTAATAATTAAACTTACCTTCAGACATTGTTGAACAAATTTAATAAATTATTTATAGCAAATTTTTG
ATTAATCCTGTATGACACTA

Product: inosine/uridine-preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSKKLVLMDHDGAVDDFLSTLLLMTMENIQPLGVIVTPADCYIEPAISATRKILDLIGRSDIPVAESTVRGVNPFPVLFR
RDSFAVDHFPILNQKENINTILISEPGQNFMVKVLKSAPAPVTLMVTGPLTTVATALDIAPEIESKIQEIVWMGGALNVP
GNVSKDMEPGQDMSAEWNAYWDPFAIERVWQTQIPIVLCSLDITNNVPLTSEFSHKLSKQRKYPISDFAGQCYALAISQD
YYFWDVLATSYLAHPEFYQLQEWETVVITKGLSQGRIKVENGGRKIMAMETVDKERFYTYILQQWAR

Sequences:

>Translated_307_residues
MSKKLVLMDHDGAVDDFLSTLLLMTMENIQPLGVIVTPADCYIEPAISATRKILDLIGRSDIPVAESTVRGVNPFPVLFR
RDSFAVDHFPILNQKENINTILISEPGQNFMVKVLKSAPAPVTLMVTGPLTTVATALDIAPEIESKIQEIVWMGGALNVP
GNVSKDMEPGQDMSAEWNAYWDPFAIERVWQTQIPIVLCSLDITNNVPLTSEFSHKLSKQRKYPISDFAGQCYALAISQD
YYFWDVLATSYLAHPEFYQLQEWETVVITKGLSQGRIKVENGGRKIMAMETVDKERFYTYILQQWAR
>Mature_306_residues
SKKLVLMDHDGAVDDFLSTLLLMTMENIQPLGVIVTPADCYIEPAISATRKILDLIGRSDIPVAESTVRGVNPFPVLFRR
DSFAVDHFPILNQKENINTILISEPGQNFMVKVLKSAPAPVTLMVTGPLTTVATALDIAPEIESKIQEIVWMGGALNVPG
NVSKDMEPGQDMSAEWNAYWDPFAIERVWQTQIPIVLCSLDITNNVPLTSEFSHKLSKQRKYPISDFAGQCYALAISQDY
YFWDVLATSYLAHPEFYQLQEWETVVITKGLSQGRIKVENGGRKIMAMETVDKERFYTYILQQWAR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=312, Percent_Identity=27.5641025641026, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786213, Length=159, Percent_Identity=30.188679245283, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788486, Length=297, Percent_Identity=21.8855218855219, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34551; Mature: 34420

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLVLMDHDGAVDDFLSTLLLMTMENIQPLGVIVTPADCYIEPAISATRKILDLIGRS
CCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHEECHHHHHHHHHHHHHCCC
DIPVAESTVRGVNPFPVLFRRDSFAVDHFPILNQKENINTILISEPGQNFMVKVLKSAPA
CCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCC
PVTLMVTGPLTTVATALDIAPEIESKIQEIVWMGGALNVPGNVSKDMEPGQDMSAEWNAY
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
WDPFAIERVWQTQIPIVLCSLDITNNVPLTSEFSHKLSKQRKYPISDFAGQCYALAISQD
CCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHCCCEEEEEEECC
YYFWDVLATSYLAHPEFYQLQEWETVVITKGLSQGRIKVENGGRKIMAMETVDKERFYTY
CCHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCCEEEEECCCCEEEEEECCCHHHHHHH
ILQQWAR
HHHHHCC
>Mature Secondary Structure 
SKKLVLMDHDGAVDDFLSTLLLMTMENIQPLGVIVTPADCYIEPAISATRKILDLIGRS
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHEECHHHHHHHHHHHHHCCC
DIPVAESTVRGVNPFPVLFRRDSFAVDHFPILNQKENINTILISEPGQNFMVKVLKSAPA
CCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCC
PVTLMVTGPLTTVATALDIAPEIESKIQEIVWMGGALNVPGNVSKDMEPGQDMSAEWNAY
CEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
WDPFAIERVWQTQIPIVLCSLDITNNVPLTSEFSHKLSKQRKYPISDFAGQCYALAISQD
CCCHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHCCCEEEEEEECC
YYFWDVLATSYLAHPEFYQLQEWETVVITKGLSQGRIKVENGGRKIMAMETVDKERFYTY
CCHHHHHHHHHHCCCCCEECCCCCEEEEECCCCCCEEEEECCCCEEEEEECCCHHHHHHH
ILQQWAR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA