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The map label for this gene is hpcH [H]

Identifier: 113476409

GI number: 113476409

Start: 4385468

End: 4386232

Strand: Reverse

Name: hpcH [H]

Synonym: Tery_2821

Alternate gene names: 113476409

Gene position: 4386232-4385468 (Counterclockwise)

Preceding gene: 113476410

Following gene: 113476408

Centisome position: 56.6

GC content: 36.6

Gene sequence:

>765_bases
ATGCCTAAAAATCAACTCAAACAAAAGCTAAAAAATGGTGAAGTTGTACTCGGACCTTTTATGAATTGTGCTTACCCTGC
ATTCGTAGAAATTTGTGGTCTAGCAGGATTTGATTTTGTAGTTATTGACCTAGAACACGGACCTTTAAATATTCTAGTTG
CAGAAGACCTTTGTCGTGCTGCTGACTGTGTAGGTATTGCTCCTGTTGTCAGAGTCAGCAAAAACGATCCTGCACAAATT
CAACGGGCTCTTGATATTGGTAGTACGGGAGTACAAGTTCCACAAATAGAAACAATAAGTGATGCAGAAATAGTAGTACA
AAGTGCTAAATATAATCCTATTGGTTGCCGTGGTTTATCATTTGGAACTCGTGCAGGACTTTACACTTCAGCAGGTAGCA
ATATTACTAATAAATTAAATGCAGAATCTTTAGTTGTAGTTCATGTAGAAGGTACAAAAGGCATCGAAAATATGGAAGAA
ATTGTTAAAGTTCCTGAGATTGATGTGATTTTTCTCGGTCCTTATGACTTATCCCAGTCTTTAGGTATTCCTGGTCAAGT
TAATGATATTCGAGTAGTTGAGTTAATGCAAAAAGCGGTAGAAATTATTCGGAGTTCTGGTAAAGTTGTCGGTACTTTTA
CTAATAATTCTGAGGCAGCAAAAAAATGGATTGACTGTGGAGTGCAGTATATTGGATTAGGTATAGATGTGAGTATTTTT
CTCCAAGGTTGTCAAGCTTTAGTTAAAGGGGTAAGAGGTAAATAA

Upstream 100 bases:

>100_bases
TCCAAAAATTGAAAATTCCTGGTAGGGTTTTGAAATAAGAGTTGGGAGACCAAAATTCTAGACTCTACTAATATTGTTTT
TCGCTTATATTCAATTAACC

Downstream 100 bases:

>100_bases
GGTGTTATATTTTAGATGCTATATTTTAGCTATTAGATGTAATATTTCAGGATAAATTATTTTCTCTTACATTCATTCAG
TATTCATAATAAGGAGTCAT

Product: HpcH/HpaI aldolase

Products: 2-Hydroxy-3-oxopropanoate; Pyruvate [C]

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MPKNQLKQKLKNGEVVLGPFMNCAYPAFVEICGLAGFDFVVIDLEHGPLNILVAEDLCRAADCVGIAPVVRVSKNDPAQI
QRALDIGSTGVQVPQIETISDAEIVVQSAKYNPIGCRGLSFGTRAGLYTSAGSNITNKLNAESLVVVHVEGTKGIENMEE
IVKVPEIDVIFLGPYDLSQSLGIPGQVNDIRVVELMQKAVEIIRSSGKVVGTFTNNSEAAKKWIDCGVQYIGLGIDVSIF
LQGCQALVKGVRGK

Sequences:

>Translated_254_residues
MPKNQLKQKLKNGEVVLGPFMNCAYPAFVEICGLAGFDFVVIDLEHGPLNILVAEDLCRAADCVGIAPVVRVSKNDPAQI
QRALDIGSTGVQVPQIETISDAEIVVQSAKYNPIGCRGLSFGTRAGLYTSAGSNITNKLNAESLVVVHVEGTKGIENMEE
IVKVPEIDVIFLGPYDLSQSLGIPGQVNDIRVVELMQKAVEIIRSSGKVVGTFTNNSEAAKKWIDCGVQYIGLGIDVSIF
LQGCQALVKGVRGK
>Mature_253_residues
PKNQLKQKLKNGEVVLGPFMNCAYPAFVEICGLAGFDFVVIDLEHGPLNILVAEDLCRAADCVGIAPVVRVSKNDPAQIQ
RALDIGSTGVQVPQIETISDAEIVVQSAKYNPIGCRGLSFGTRAGLYTSAGSNITNKLNAESLVVVHVEGTKGIENMEEI
VKVPEIDVIFLGPYDLSQSLGIPGQVNDIRVVELMQKAVEIIRSSGKVVGTFTNNSEAAKKWIDCGVQYIGLGIDVSIFL
QGCQALVKGVRGK

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=256, Percent_Identity=31.640625, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788578, Length=237, Percent_Identity=28.2700421940928, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.20 [C]

Molecular weight: Translated: 27156; Mature: 27025

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKNQLKQKLKNGEVVLGPFMNCAYPAFVEICGLAGFDFVVIDLEHGPLNILVAEDLCRA
CCHHHHHHHHCCCCEEEECHHHCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHHH
ADCVGIAPVVRVSKNDPAQIQRALDIGSTGVQVPQIETISDAEIVVQSAKYNPIGCRGLS
HHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECCCCCCCCCCCCC
FGTRAGLYTSAGSNITNKLNAESLVVVHVEGTKGIENMEEIVKVPEIDVIFLGPYDLSQS
CCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCHHC
LGIPGQVNDIRVVELMQKAVEIIRSSGKVVGTFTNNSEAAKKWIDCGVQYIGLGIDVSIF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCEEEECCCHHHHH
LQGCQALVKGVRGK
HHHHHHHHHHCCCC
>Mature Secondary Structure 
PKNQLKQKLKNGEVVLGPFMNCAYPAFVEICGLAGFDFVVIDLEHGPLNILVAEDLCRA
CHHHHHHHHCCCCEEEECHHHCCHHHHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHHH
ADCVGIAPVVRVSKNDPAQIQRALDIGSTGVQVPQIETISDAEIVVQSAKYNPIGCRGLS
HHHCCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECCCCCCCCCCCCC
FGTRAGLYTSAGSNITNKLNAESLVVVHVEGTKGIENMEEIVKVPEIDVIFLGPYDLSQS
CCCCCCEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCHHC
LGIPGQVNDIRVVELMQKAVEIIRSSGKVVGTFTNNSEAAKKWIDCGVQYIGLGIDVSIF
CCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCEEEECCCHHHHH
LQGCQALVKGVRGK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-Dehydro-4-deoxy-D-glucarate [C]

Specific reaction: 5-Dehydro-4-deoxy-D-glucarate --> 2-Hydroxy-3-oxopropanoate + Pyruvate [C]

General reaction: Carbon-Carbon Lyases; Aldehyde-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA