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The map label for this gene is spoT [H]

Identifier: 113476259

GI number: 113476259

Start: 4110199

End: 4112451

Strand: Reverse

Name: spoT [H]

Synonym: Tery_2653

Alternate gene names: 113476259

Gene position: 4112451-4110199 (Counterclockwise)

Preceding gene: 113476264

Following gene: 113476258

Centisome position: 53.06

GC content: 33.47

Gene sequence:

>2253_bases
ATGAAAACAAAAACCGTCACACCGAAAGCTCAAATTTCATGTGTAATTCCTGATTGGTTGCAAGAATGTCTAGATAACAG
AAATAAGGGCAATAATGTTACTAGTTGTTCAGTGGATACAGCATTAATTTGTCGAGCATTTGAATTTGCATATAAGTTAC
ATGAAGGGCAGTATAGAAAATCAGGAGAACCTTATATTTGTCATCCTGTAGCCGTAGCAGGATTATTAAAATATTTAGGT
GGTGATAGTACCATGATTGCTGCAGGTTTGCTACATGATATTGTTGAAGATACTGACGTAAAAATTGAAGAAATAGAGGA
AAAATTTGGTTCAGAAGTAACTTTATTAGTAGAAGGTGTAACTAAACTTTCTAAGTTTAATTTTTCTAATAAAACAGAGC
TACAAGCTGAAAATTTTCGCCGAATGTTTTTGGCAATGGCAAAAGATATTAGAGTCATTGTTGTCAAGTTGGCAGACAGA
TTACATAATATGAGAACTCTCCAACATTTACCTTCTCATAAACAGTTAAGTAAAGCATTAGAAACACGGGAAATATTTGC
ACCACTGGCAAATCGTTTAGGAATTGGTAGGATTAAATGGGAATTAGAAGATTTATCTTTCAAATATCTTGAACCAAATG
CTTATCGTGAAATTCAAGAGTTGGTAGCAGATAAAAGGGTAGATAGGGAAGCTAGATTATTAAAAATGACAGAAAATTTA
CGACAAAAATTAGACCGCTTAGGAATAGAATGTTACGACATTAGTGGTCGCCCTAAACATCTTTATGGAATTTATCGAAA
GATGTACTTAAAACAAAAAGAATTTCACGAAGTATATGATATTTCTGGACTGAGAATTATTGTAGAAAAAAGAGAAGATT
GTTATCGAACTTTAGCTATTGTTCATGACTCTTATCGTCCTATTCCTGGAAGATTTAAAGACTATATTGGTCTGCCAAAA
CCTAATCGTTATCAGTCGTTACATACAGGAGTAATAGGTATTACAGGTCGTCCGGTAGAGGTGCAAATTAGAACTGTGGA
AATGCACCATGTTGCTGAGTATGGAATAGCTGCTCATTGGAAGTATAAAGAGACTGGTTATAGTAATACTAATGTTAATA
TTACGACAGAAGATGAAAAGTTTACTTGGCTTAGACAGTTATTAGAATGGCAAAGTGACCTGAAAGATGATCGGGAATAT
TTGGAAACTATTAAGGGTGATTTATTCTCTGAAGATGTTTATGCATTTACACCAAAAGGTGATGTTATTTCCCTCAAACA
TGGATCGACACCAATAGATTTCGCTTATCGTATTCATACAGAGGTAGGAAATCATTGTACAGGTGCTAAGGTAAATGAAC
GAATAGTACCTTTAGATACTCCTTTAAAAAATGGTGATATTGTCGAAATTTTAACTCATAAAAATGGCCATCCTAGCTTA
GATTGGCTAAATTCTGTGGTTACTGGTGGAGCACGAAATCGAATTCGTCAGTGGTATAAGCGTTCCCATAGAGAAGAAAT
TTTAGCTAGAGGTCGAGAATTATTAGAAAAAGAATTGGGTAAAAAAGGTTTTGAATCTTTACTCAAATCTGAGCAAATGC
GACTTGTTTCTGAAAAATTTAATTATCATAATGTAGATGATTTATTGGCTGGTTTGGGATATGGTGAAATAACTTTAAAT
TTAGTGGTCAATCGATTGCGAGATGCTTTTAGAGAAGTCACAAAAGTTGAGGAAAATTCAGATATTTGTTCAGAAAAATA
TTTACACAAGTCTACTCAAATTTCGTCTCCTAATAATTATCATCCAATCGCTGGAGTTGAAGGGTTAATGTATCATTTAG
CAGGTTGTTGTAATCCGATTCCAGGTGAGCCTATTATTGGAGCTGTAACTCGTATAAATGGTATTTCTATTCATCGTCAA
GGTTGTTCTAATATAGAAAATGTACCTGGAGAAAGGTTAGTTCCTGTTAGTTGGAATAATGCTAATGAAAATAATGGAAG
ACCGAAAACTTATCCAGTTAATATCTATATTGAAGTTATAGATAGAGTGGGTGTTCTCAAAGATATTTTATCCCGTTTGA
TGGATAATAATGTTAATGTTAAAAATGCTCAGGTTAAAACAAATCCTGGTCATACTGCTATTATTAATCTATGTATTGAA
GTGAGCGACTGCCAACAATTTGAACGTAGTTGTACTCAGATTAAAAATATAAGTGATATTTTGAATTTACGTCGTGTTAG
TCAGGTTGAATAG

Upstream 100 bases:

>100_bases
TGGGAAGATTTTGGAATTGTAAAAGTAGGATTGCTATATATAGCTGTTTTATACAGAAATAAACTAATATTTGATACTTA
TTAACTCTAAACTAATTACT

Downstream 100 bases:

>100_bases
TATTTTTAGTTAGGAGGAATAAGTAGTAAGGAAAAGTCAAATGTAAGTATTCAGCTTTTAAGTATTAAAGGAATTACTGA
AGGTGAGCTGAACGCCTTTT

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 750; Mature: 750

Protein sequence:

>750_residues
MKTKTVTPKAQISCVIPDWLQECLDNRNKGNNVTSCSVDTALICRAFEFAYKLHEGQYRKSGEPYICHPVAVAGLLKYLG
GDSTMIAAGLLHDIVEDTDVKIEEIEEKFGSEVTLLVEGVTKLSKFNFSNKTELQAENFRRMFLAMAKDIRVIVVKLADR
LHNMRTLQHLPSHKQLSKALETREIFAPLANRLGIGRIKWELEDLSFKYLEPNAYREIQELVADKRVDREARLLKMTENL
RQKLDRLGIECYDISGRPKHLYGIYRKMYLKQKEFHEVYDISGLRIIVEKREDCYRTLAIVHDSYRPIPGRFKDYIGLPK
PNRYQSLHTGVIGITGRPVEVQIRTVEMHHVAEYGIAAHWKYKETGYSNTNVNITTEDEKFTWLRQLLEWQSDLKDDREY
LETIKGDLFSEDVYAFTPKGDVISLKHGSTPIDFAYRIHTEVGNHCTGAKVNERIVPLDTPLKNGDIVEILTHKNGHPSL
DWLNSVVTGGARNRIRQWYKRSHREEILARGRELLEKELGKKGFESLLKSEQMRLVSEKFNYHNVDDLLAGLGYGEITLN
LVVNRLRDAFREVTKVEENSDICSEKYLHKSTQISSPNNYHPIAGVEGLMYHLAGCCNPIPGEPIIGAVTRINGISIHRQ
GCSNIENVPGERLVPVSWNNANENNGRPKTYPVNIYIEVIDRVGVLKDILSRLMDNNVNVKNAQVKTNPGHTAIINLCIE
VSDCQQFERSCTQIKNISDILNLRRVSQVE

Sequences:

>Translated_750_residues
MKTKTVTPKAQISCVIPDWLQECLDNRNKGNNVTSCSVDTALICRAFEFAYKLHEGQYRKSGEPYICHPVAVAGLLKYLG
GDSTMIAAGLLHDIVEDTDVKIEEIEEKFGSEVTLLVEGVTKLSKFNFSNKTELQAENFRRMFLAMAKDIRVIVVKLADR
LHNMRTLQHLPSHKQLSKALETREIFAPLANRLGIGRIKWELEDLSFKYLEPNAYREIQELVADKRVDREARLLKMTENL
RQKLDRLGIECYDISGRPKHLYGIYRKMYLKQKEFHEVYDISGLRIIVEKREDCYRTLAIVHDSYRPIPGRFKDYIGLPK
PNRYQSLHTGVIGITGRPVEVQIRTVEMHHVAEYGIAAHWKYKETGYSNTNVNITTEDEKFTWLRQLLEWQSDLKDDREY
LETIKGDLFSEDVYAFTPKGDVISLKHGSTPIDFAYRIHTEVGNHCTGAKVNERIVPLDTPLKNGDIVEILTHKNGHPSL
DWLNSVVTGGARNRIRQWYKRSHREEILARGRELLEKELGKKGFESLLKSEQMRLVSEKFNYHNVDDLLAGLGYGEITLN
LVVNRLRDAFREVTKVEENSDICSEKYLHKSTQISSPNNYHPIAGVEGLMYHLAGCCNPIPGEPIIGAVTRINGISIHRQ
GCSNIENVPGERLVPVSWNNANENNGRPKTYPVNIYIEVIDRVGVLKDILSRLMDNNVNVKNAQVKTNPGHTAIINLCIE
VSDCQQFERSCTQIKNISDILNLRRVSQVE
>Mature_750_residues
MKTKTVTPKAQISCVIPDWLQECLDNRNKGNNVTSCSVDTALICRAFEFAYKLHEGQYRKSGEPYICHPVAVAGLLKYLG
GDSTMIAAGLLHDIVEDTDVKIEEIEEKFGSEVTLLVEGVTKLSKFNFSNKTELQAENFRRMFLAMAKDIRVIVVKLADR
LHNMRTLQHLPSHKQLSKALETREIFAPLANRLGIGRIKWELEDLSFKYLEPNAYREIQELVADKRVDREARLLKMTENL
RQKLDRLGIECYDISGRPKHLYGIYRKMYLKQKEFHEVYDISGLRIIVEKREDCYRTLAIVHDSYRPIPGRFKDYIGLPK
PNRYQSLHTGVIGITGRPVEVQIRTVEMHHVAEYGIAAHWKYKETGYSNTNVNITTEDEKFTWLRQLLEWQSDLKDDREY
LETIKGDLFSEDVYAFTPKGDVISLKHGSTPIDFAYRIHTEVGNHCTGAKVNERIVPLDTPLKNGDIVEILTHKNGHPSL
DWLNSVVTGGARNRIRQWYKRSHREEILARGRELLEKELGKKGFESLLKSEQMRLVSEKFNYHNVDDLLAGLGYGEITLN
LVVNRLRDAFREVTKVEENSDICSEKYLHKSTQISSPNNYHPIAGVEGLMYHLAGCCNPIPGEPIIGAVTRINGISIHRQ
GCSNIENVPGERLVPVSWNNANENNGRPKTYPVNIYIEVIDRVGVLKDILSRLMDNNVNVKNAQVKTNPGHTAIINLCIE
VSDCQQFERSCTQIKNISDILNLRRVSQVE

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=133, Percent_Identity=39.0977443609023, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1790082, Length=706, Percent_Identity=39.5184135977337, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1789147, Length=721, Percent_Identity=37.0319001386963, Blast_Score=495, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25143118, Length=134, Percent_Identity=35.0746268656716, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24650996, Length=133, Percent_Identity=34.5864661654135, Blast_Score=72, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 85780; Mature: 85780

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKTVTPKAQISCVIPDWLQECLDNRNKGNNVTSCSVDTALICRAFEFAYKLHEGQYRK
CCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCC
SGEPYICHPVAVAGLLKYLGGDSTMIAAGLLHDIVEDTDVKIEEIEEKFGSEVTLLVEGV
CCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEHHHH
TKLSKFNFSNKTELQAENFRRMFLAMAKDIRVIVVKLADRLHNMRTLQHLPSHKQLSKAL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ETREIFAPLANRLGIGRIKWELEDLSFKYLEPNAYREIQELVADKRVDREARLLKMTENL
HHHHHHHHHHHHCCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RQKLDRLGIECYDISGRPKHLYGIYRKMYLKQKEFHEVYDISGLRIIVEKREDCYRTLAI
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
VHDSYRPIPGRFKDYIGLPKPNRYQSLHTGVIGITGRPVEVQIRTVEMHHVAEYGIAAHW
HHCCCCCCCCHHHHHCCCCCCCCHHHHHCCEEEECCCCEEEEEEEEEHHHHHHHCCEEEE
KYKETGYSNTNVNITTEDEKFTWLRQLLEWQSDLKDDREYLETIKGDLFSEDVYAFTPKG
EECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
DVISLKHGSTPIDFAYRIHTEVGNHCTGAKVNERIVPLDTPLKNGDIVEILTHKNGHPSL
CEEEEECCCCCCEEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCH
DWLNSVVTGGARNRIRQWYKRSHREEILARGRELLEKELGKKGFESLLKSEQMRLVSEKF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
NYHNVDDLLAGLGYGEITLNLVVNRLRDAFREVTKVEENSDICSEKYLHKSTQISSPNNY
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
HPIAGVEGLMYHLAGCCNPIPGEPIIGAVTRINGISIHRQGCSNIENVPGERLVPVSWNN
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCC
ANENNGRPKTYPVNIYIEVIDRVGVLKDILSRLMDNNVNVKNAQVKTNPGHTAIINLCIE
CCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEE
VSDCQQFERSCTQIKNISDILNLRRVSQVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTKTVTPKAQISCVIPDWLQECLDNRNKGNNVTSCSVDTALICRAFEFAYKLHEGQYRK
CCCCCCCCCCEEEEECHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCC
SGEPYICHPVAVAGLLKYLGGDSTMIAAGLLHDIVEDTDVKIEEIEEKFGSEVTLLVEGV
CCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEHHHH
TKLSKFNFSNKTELQAENFRRMFLAMAKDIRVIVVKLADRLHNMRTLQHLPSHKQLSKAL
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ETREIFAPLANRLGIGRIKWELEDLSFKYLEPNAYREIQELVADKRVDREARLLKMTENL
HHHHHHHHHHHHCCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RQKLDRLGIECYDISGRPKHLYGIYRKMYLKQKEFHEVYDISGLRIIVEKREDCYRTLAI
HHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHH
VHDSYRPIPGRFKDYIGLPKPNRYQSLHTGVIGITGRPVEVQIRTVEMHHVAEYGIAAHW
HHCCCCCCCCHHHHHCCCCCCCCHHHHHCCEEEECCCCEEEEEEEEEHHHHHHHCCEEEE
KYKETGYSNTNVNITTEDEKFTWLRQLLEWQSDLKDDREYLETIKGDLFSEDVYAFTPKG
EECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
DVISLKHGSTPIDFAYRIHTEVGNHCTGAKVNERIVPLDTPLKNGDIVEILTHKNGHPSL
CEEEEECCCCCCEEEEEEEHHHCCCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCCCCH
DWLNSVVTGGARNRIRQWYKRSHREEILARGRELLEKELGKKGFESLLKSEQMRLVSEKF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHC
NYHNVDDLLAGLGYGEITLNLVVNRLRDAFREVTKVEENSDICSEKYLHKSTQISSPNNY
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
HPIAGVEGLMYHLAGCCNPIPGEPIIGAVTRINGISIHRQGCSNIENVPGERLVPVSWNN
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCC
ANENNGRPKTYPVNIYIEVIDRVGVLKDILSRLMDNNVNVKNAQVKTNPGHTAIINLCIE
CCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEEEE
VSDCQQFERSCTQIKNISDILNLRRVSQVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]