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The map label for this gene is slt [H]

Identifier: 113475743

GI number: 113475743

Start: 3263487

End: 3265649

Strand: Reverse

Name: slt [H]

Synonym: Tery_2092

Alternate gene names: 113475743

Gene position: 3265649-3263487 (Counterclockwise)

Preceding gene: 113475745

Following gene: 113475740

Centisome position: 42.14

GC content: 37.77

Gene sequence:

>2163_bases
ATGTTAAAGCAAAACATAACAAAAGTTTCTATAGTAATAGTCGCTTCTGTTAGCGCTCTGTTATCGGGGGTTTTTCTGGT
ACAATTTACTAGCAATAACGAAAACTCCCAAAAGACTACCCAAAAAGAAAAACTTAATCAAGAGTTAAATGTAGTAAGTT
TTGTGTCACTGCAACCAGAGGAACGTCGTCGTAGGTTGGAAACTGCGATCAAAACTGGAAAATCTCTCAACACTAGTCGT
GCTCGTTATGTTCTAGCTAATGATTTAATTCAACAGGGAGAGGCTGAGTTGGCGATCGCTCAACTGAAAGATTTAGAAAA
AGAATATATAACTCTAGCACAATATATTCTAGTTAAACGAGCGCAAGCTTACGAACAAATAGGGGATACAGACAAAGCAA
AAAGAGTTTGGCAAGAAGTTTTAAGATACGATCCACAAGAAGCTGTGGTGGTAGAAGCTTTGTATATACTAGGAAAAGAA
AACCCGGAATACTGGGATGAGGCGATCGCTAAATTTCCTGGTTATCCAGCTACAGTTAAAATTGCTCAGGAAAAATTAAA
GCAAAATCCTAATCAACCTCGGTTATTATTGCTTATTGCTAAGTATGGTTTTCATGTGCCTGAATATAGCACGGTATTAG
AACAACTTAGAACAAAATATGCTTCTGAGTTGACTCCCGAAGATTGGGAAATGGTTGCTTTTGGGTATTGGGAAAAACAA
GATTATGGTAAAGGTGCCTTAGCATATCAGAAAGCAACCAAGACTCCCCAAAATTTATATCGTTATGCCAGGGGCTTATG
GTTAGGAGGAAAAATCAAAGAATCTCGCGAAGCTTATAAAACTTTAATTAATACATTTCCTAATGGCGGAGAAGATACAG
CTTTGGGTTTAATTCGTTTGTCGAGGTTAGTTGATCGAAAAGATGCTATACCTTATCTTGACAGGGTAATTGTTAACTTC
CCCAGTTTAGCACCCGAAGCATTGTTAGATAAGTCTAAGTTGCTTGATAAACTTGACTCTACTAAGTCAGCTTCTCTGTT
ACGTCAACAATTGTTGAGAGAATATAGTAATTCTGATGCTGCGGCTATACTACGCTGGAAGTTAGCTGAACAAGCTGCCG
CAGGTGGTAATTTACAAGTGGCTTGGAAGTGGGCGCAAGAGTTGACTGTGAATAATCCTGATCATAAGTTGGCAGCGCAA
GCAGGTTTCTGGGTAGGTAAGTGGGCACAACAACTTGGGCGGACAGAGGATGCCACAAAAGCTTTTGAGTATATGATTTT
ACGATATCCTCACTCTTATTATGCTTGGCGATCGGCAGTGATGCTAGGTTGGCCTGTAGGGGATTTTACGACAGTTAGGA
ATTTATCTCCAGAGGTTGTACAAAGGAAAGACCGTGAACCACTGCCTTTTGGTTCTGAAACATTGCGGGAACTATATCAG
ATTGGACAAGACTGGGATGCTTGGAAACAGTGGCAAGTAGAATTTAAAGACCGCATGAATCCAACTGTCGCTCAACAGTA
TACTGATGGCGTATTGAGGGTTGGTGTGGGGGATAATTTGGATGGTATTTGGATGCTAACTAGCTTGAGTAAATGGGAAT
CTCCAGAGGAGATAGCTGAGTATAAAGCTTTAAAAGAAAAGTCTACTTATTGGCAAACTTTGTATCCTTTTCCTTATTTG
GAAACTATTGTTAAATGGTCTAATAAACGAAAACTAAATACTGTTTTGGTGACTGCTTTAATTCGTCAAGAATCTCGTTT
TATGCCAAACATCAAATCAGTTGTGGGAGCAACAGGACTAATGCAGATAATGCCACAAACTGGTAAAGAGGCTGCTAAGA
AAATTAATCTTGTTAGTTATGATTTAGAGAATATTAATGATAATGTTAATTTAGGTACTTATTACTTGGATTTTACTCAT
AAAAAGTATAAGAATAATTCAATGTTGGCAATTGCTAGTTATAATGCCGGTCCGAATGCTATAAATAGTTGGTTAAAACG
TTTTGGGTTTGATGATGCTGATGCTTTTGTGGAGAAAATTCCTTATCCAGAAACAAGGGGTTATGTGGAGTCTGTCTTTG
AAAATTATTGGAATTATTTGCAGATTTATAACCCTGAAGTTTCTCAGTTAATGGAAAAACATTATGCTAAATATAATCAA
TAA

Upstream 100 bases:

>100_bases
ACTTTTTCTAGGAGTGTAATCTTCAATTCATACTTTAAATTGTCCCTAGGGGAGAAAATTAACACTTGAAAAAAATTGAA
AATTAACTGATGGCAAGTAA

Downstream 100 bases:

>100_bases
AACTAATTAATCTTCCTTATAGTGTGCCTTCGGTCAGTTTTTGCTTATCCATAACCCCAGACTTTAACCACTGTTTAATC
AATAGCCTATATAGTGTATC

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 720; Mature: 720

Protein sequence:

>720_residues
MLKQNITKVSIVIVASVSALLSGVFLVQFTSNNENSQKTTQKEKLNQELNVVSFVSLQPEERRRRLETAIKTGKSLNTSR
ARYVLANDLIQQGEAELAIAQLKDLEKEYITLAQYILVKRAQAYEQIGDTDKAKRVWQEVLRYDPQEAVVVEALYILGKE
NPEYWDEAIAKFPGYPATVKIAQEKLKQNPNQPRLLLLIAKYGFHVPEYSTVLEQLRTKYASELTPEDWEMVAFGYWEKQ
DYGKGALAYQKATKTPQNLYRYARGLWLGGKIKESREAYKTLINTFPNGGEDTALGLIRLSRLVDRKDAIPYLDRVIVNF
PSLAPEALLDKSKLLDKLDSTKSASLLRQQLLREYSNSDAAAILRWKLAEQAAAGGNLQVAWKWAQELTVNNPDHKLAAQ
AGFWVGKWAQQLGRTEDATKAFEYMILRYPHSYYAWRSAVMLGWPVGDFTTVRNLSPEVVQRKDREPLPFGSETLRELYQ
IGQDWDAWKQWQVEFKDRMNPTVAQQYTDGVLRVGVGDNLDGIWMLTSLSKWESPEEIAEYKALKEKSTYWQTLYPFPYL
ETIVKWSNKRKLNTVLVTALIRQESRFMPNIKSVVGATGLMQIMPQTGKEAAKKINLVSYDLENINDNVNLGTYYLDFTH
KKYKNNSMLAIASYNAGPNAINSWLKRFGFDDADAFVEKIPYPETRGYVESVFENYWNYLQIYNPEVSQLMEKHYAKYNQ

Sequences:

>Translated_720_residues
MLKQNITKVSIVIVASVSALLSGVFLVQFTSNNENSQKTTQKEKLNQELNVVSFVSLQPEERRRRLETAIKTGKSLNTSR
ARYVLANDLIQQGEAELAIAQLKDLEKEYITLAQYILVKRAQAYEQIGDTDKAKRVWQEVLRYDPQEAVVVEALYILGKE
NPEYWDEAIAKFPGYPATVKIAQEKLKQNPNQPRLLLLIAKYGFHVPEYSTVLEQLRTKYASELTPEDWEMVAFGYWEKQ
DYGKGALAYQKATKTPQNLYRYARGLWLGGKIKESREAYKTLINTFPNGGEDTALGLIRLSRLVDRKDAIPYLDRVIVNF
PSLAPEALLDKSKLLDKLDSTKSASLLRQQLLREYSNSDAAAILRWKLAEQAAAGGNLQVAWKWAQELTVNNPDHKLAAQ
AGFWVGKWAQQLGRTEDATKAFEYMILRYPHSYYAWRSAVMLGWPVGDFTTVRNLSPEVVQRKDREPLPFGSETLRELYQ
IGQDWDAWKQWQVEFKDRMNPTVAQQYTDGVLRVGVGDNLDGIWMLTSLSKWESPEEIAEYKALKEKSTYWQTLYPFPYL
ETIVKWSNKRKLNTVLVTALIRQESRFMPNIKSVVGATGLMQIMPQTGKEAAKKINLVSYDLENINDNVNLGTYYLDFTH
KKYKNNSMLAIASYNAGPNAINSWLKRFGFDDADAFVEKIPYPETRGYVESVFENYWNYLQIYNPEVSQLMEKHYAKYNQ
>Mature_720_residues
MLKQNITKVSIVIVASVSALLSGVFLVQFTSNNENSQKTTQKEKLNQELNVVSFVSLQPEERRRRLETAIKTGKSLNTSR
ARYVLANDLIQQGEAELAIAQLKDLEKEYITLAQYILVKRAQAYEQIGDTDKAKRVWQEVLRYDPQEAVVVEALYILGKE
NPEYWDEAIAKFPGYPATVKIAQEKLKQNPNQPRLLLLIAKYGFHVPEYSTVLEQLRTKYASELTPEDWEMVAFGYWEKQ
DYGKGALAYQKATKTPQNLYRYARGLWLGGKIKESREAYKTLINTFPNGGEDTALGLIRLSRLVDRKDAIPYLDRVIVNF
PSLAPEALLDKSKLLDKLDSTKSASLLRQQLLREYSNSDAAAILRWKLAEQAAAGGNLQVAWKWAQELTVNNPDHKLAAQ
AGFWVGKWAQQLGRTEDATKAFEYMILRYPHSYYAWRSAVMLGWPVGDFTTVRNLSPEVVQRKDREPLPFGSETLRELYQ
IGQDWDAWKQWQVEFKDRMNPTVAQQYTDGVLRVGVGDNLDGIWMLTSLSKWESPEEIAEYKALKEKSTYWQTLYPFPYL
ETIVKWSNKRKLNTVLVTALIRQESRFMPNIKSVVGATGLMQIMPQTGKEAAKKINLVSYDLENINDNVNLGTYYLDFTH
KKYKNNSMLAIASYNAGPNAINSWLKRFGFDDADAFVEKIPYPETRGYVESVFENYWNYLQIYNPEVSQLMEKHYAKYNQ

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=165, Percent_Identity=36.3636363636364, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 82766; Mature: 82766

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS50005 TPR ; PS50293 TPR_REGION ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQNITKVSIVIVASVSALLSGVFLVQFTSNNENSQKTTQKEKLNQELNVVSFVSLQPE
CCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCH
ERRRRLETAIKTGKSLNTSRARYVLANDLIQQGEAELAIAQLKDLEKEYITLAQYILVKR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAYEQIGDTDKAKRVWQEVLRYDPQEAVVVEALYILGKENPEYWDEAIAKFPGYPATVK
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
IAQEKLKQNPNQPRLLLLIAKYGFHVPEYSTVLEQLRTKYASELTPEDWEMVAFGYWEKQ
HHHHHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
DYGKGALAYQKATKTPQNLYRYARGLWLGGKIKESREAYKTLINTFPNGGEDTALGLIRL
CCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SRLVDRKDAIPYLDRVIVNFPSLAPEALLDKSKLLDKLDSTKSASLLRQQLLREYSNSDA
HHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
AAILRWKLAEQAAAGGNLQVAWKWAQELTVNNPDHKLAAQAGFWVGKWAQQLGRTEDATK
HHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHH
AFEYMILRYPHSYYAWRSAVMLGWPVGDFTTVRNLSPEVVQRKDREPLPFGSETLRELYQ
HHHHHHHHCCCHHHHHHHHEEEECCCCCHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHH
IGQDWDAWKQWQVEFKDRMNPTVAQQYTDGVLRVGVGDNLDGIWMLTSLSKWESPEEIAE
HCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCEEEECCCHHCCCHHHHHH
YKALKEKSTYWQTLYPFPYLETIVKWSNKRKLNTVLVTALIRQESRFMPNIKSVVGATGL
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MQIMPQTGKEAAKKINLVSYDLENINDNVNLGTYYLDFTHKKYKNNSMLAIASYNAGPNA
HHHHCCCHHHHHHHHHHHEEEHHCCCCCCEEEEEEEEECHHHCCCCCEEEEEECCCCHHH
INSWLKRFGFDDADAFVEKIPYPETRGYVESVFENYWNYLQIYNPEVSQLMEKHYAKYNQ
HHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKQNITKVSIVIVASVSALLSGVFLVQFTSNNENSQKTTQKEKLNQELNVVSFVSLQPE
CCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEECCCH
ERRRRLETAIKTGKSLNTSRARYVLANDLIQQGEAELAIAQLKDLEKEYITLAQYILVKR
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAYEQIGDTDKAKRVWQEVLRYDPQEAVVVEALYILGKENPEYWDEAIAKFPGYPATVK
HHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
IAQEKLKQNPNQPRLLLLIAKYGFHVPEYSTVLEQLRTKYASELTPEDWEMVAFGYWEKQ
HHHHHHHCCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
DYGKGALAYQKATKTPQNLYRYARGLWLGGKIKESREAYKTLINTFPNGGEDTALGLIRL
CCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SRLVDRKDAIPYLDRVIVNFPSLAPEALLDKSKLLDKLDSTKSASLLRQQLLREYSNSDA
HHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
AAILRWKLAEQAAAGGNLQVAWKWAQELTVNNPDHKLAAQAGFWVGKWAQQLGRTEDATK
HHHHHHHHHHHHCCCCCEEEEEEEHHHCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHH
AFEYMILRYPHSYYAWRSAVMLGWPVGDFTTVRNLSPEVVQRKDREPLPFGSETLRELYQ
HHHHHHHHCCCHHHHHHHHEEEECCCCCHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHH
IGQDWDAWKQWQVEFKDRMNPTVAQQYTDGVLRVGVGDNLDGIWMLTSLSKWESPEEIAE
HCCCHHHHHHHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCCEEEECCCHHCCCHHHHHH
YKALKEKSTYWQTLYPFPYLETIVKWSNKRKLNTVLVTALIRQESRFMPNIKSVVGATGL
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
MQIMPQTGKEAAKKINLVSYDLENINDNVNLGTYYLDFTHKKYKNNSMLAIASYNAGPNA
HHHHCCCHHHHHHHHHHHEEEHHCCCCCCEEEEEEEEECHHHCCCCCEEEEEECCCCHHH
INSWLKRFGFDDADAFVEKIPYPETRGYVESVFENYWNYLQIYNPEVSQLMEKHYAKYNQ
HHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]