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The map label for this gene is lipA1 [H]

Identifier: 113475622

GI number: 113475622

Start: 3033921

End: 3034799

Strand: Reverse

Name: lipA1 [H]

Synonym: Tery_1957

Alternate gene names: 113475622

Gene position: 3034799-3033921 (Counterclockwise)

Preceding gene: 113475624

Following gene: 113475621

Centisome position: 39.16

GC content: 37.54

Gene sequence:

>879_bases
GTGGTAGTAAAACCAGATTGGTTAAGAGTCAAAGCTCCCCAATGGCAGAGAGTCGGTACGGTTAAAGAAATTTTGCGAGA
CTTAAGTCTCAAAACGGTCTGTGAAGAAGCTTCCTGTCCAAACATTGGTGAATGTTTTAATGCTGGGACAGCAACTTTTT
TAATTATGGGACCAGCTTGTACTCGTGCTTGTCCTTACTGTGATATTAATTTTGAGAAAAAACCTCAGCCTTTAGATGTT
AATGAACCAAAACAGCTAGCAGAAGCAGTAAAAAGATTAAGACTTAATCATGTAGTTATTACTTCTGTCAATAGGGATGA
TTTAACTGATGGAGGTGCTACTCAGTTTGCTAAATGTATTTCTCAAGTCCGGGCAATTTCACCTCAGACTACTATAGAAG
TCCTGATACCAGATTTATGTGGTAATTGGCAAGCTTTAGAAATTATTCTTAATGCTAAACCAGAAGTTATTAATCATAAT
ACTGAGACTGTTCAACGGTTATATCGCCGAGTTAGGCCTCAAGGTAATTATCATCGAACATTAGAACTGTTGAAGCGAGC
TCGAGAATTAGCCCCAGAAATATATACAAAATCTGGAATTATGGTAGGTGTCGGAGAAACTGACGCAGAAGTCCAAGAGG
TAATGAAGGACTTACGAGTTGTTGACTGTGATATTTTAACTATTGGACAATACCTACAACCAAGTCTTAAACATCTACCA
GTAGATACTTTCATCAAACCTGAACAATTTGATCAATGGAAAAAAATAGGGCTTTCAATGGGATTTTTACAAGTTGTGGC
TTCGCCCTTGACTCGTAGCTCATATCATGCTGAAGAGGTAAAAGAATTGATGGTAAATTATCCAAAGACCAAAAGTTAG

Upstream 100 bases:

>100_bases
TATAGCTCTACTATCTAAATAAATTTACTCAAAACCCTTGTTAGTAGGGTGATATATTTGTTAAGGTTAATGTGTAGATA
AAACTACAGCAGAGGAAAAA

Downstream 100 bases:

>100_bases
CAATTTACTTCTAATTTTTACCTTTTTATGAAATCTGCGAAAAGGATAGTAGCTTAGTAGGTATATAATCCATCACTCAT
GGTGGTATATCTAGTTACTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVVKPDWLRVKAPQWQRVGTVKEILRDLSLKTVCEEASCPNIGECFNAGTATFLIMGPACTRACPYCDINFEKKPQPLDV
NEPKQLAEAVKRLRLNHVVITSVNRDDLTDGGATQFAKCISQVRAISPQTTIEVLIPDLCGNWQALEIILNAKPEVINHN
TETVQRLYRRVRPQGNYHRTLELLKRARELAPEIYTKSGIMVGVGETDAEVQEVMKDLRVVDCDILTIGQYLQPSLKHLP
VDTFIKPEQFDQWKKIGLSMGFLQVVASPLTRSSYHAEEVKELMVNYPKTKS

Sequences:

>Translated_292_residues
MVVKPDWLRVKAPQWQRVGTVKEILRDLSLKTVCEEASCPNIGECFNAGTATFLIMGPACTRACPYCDINFEKKPQPLDV
NEPKQLAEAVKRLRLNHVVITSVNRDDLTDGGATQFAKCISQVRAISPQTTIEVLIPDLCGNWQALEIILNAKPEVINHN
TETVQRLYRRVRPQGNYHRTLELLKRARELAPEIYTKSGIMVGVGETDAEVQEVMKDLRVVDCDILTIGQYLQPSLKHLP
VDTFIKPEQFDQWKKIGLSMGFLQVVASPLTRSSYHAEEVKELMVNYPKTKS
>Mature_292_residues
MVVKPDWLRVKAPQWQRVGTVKEILRDLSLKTVCEEASCPNIGECFNAGTATFLIMGPACTRACPYCDINFEKKPQPLDV
NEPKQLAEAVKRLRLNHVVITSVNRDDLTDGGATQFAKCISQVRAISPQTTIEVLIPDLCGNWQALEIILNAKPEVINHN
TETVQRLYRRVRPQGNYHRTLELLKRARELAPEIYTKSGIMVGVGETDAEVQEVMKDLRVVDCDILTIGQYLQPSLKHLP
VDTFIKPEQFDQWKKIGLSMGFLQVVASPLTRSSYHAEEVKELMVNYPKTKS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=43.2624113475177, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=43.0327868852459, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=50.7142857142857, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=41.7322834645669, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=282, Percent_Identity=41.4893617021277, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI221513272, Length=281, Percent_Identity=43.4163701067616, Blast_Score=257, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 32972; Mature: 32972

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVKPDWLRVKAPQWQRVGTVKEILRDLSLKTVCEEASCPNIGECFNAGTATFLIMGPAC
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHH
TRACPYCDINFEKKPQPLDVNEPKQLAEAVKRLRLNHVVITSVNRDDLTDGGATQFAKCI
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHH
SQVRAISPQTTIEVLIPDLCGNWQALEIILNAKPEVINHNTETVQRLYRRVRPQGNYHRT
HHHHHCCCCCHHEEEEHHHCCCCEEEEEEECCCCHHHCCCHHHHHHHHHHHCCCCCHHHH
LELLKRARELAPEIYTKSGIMVGVGETDAEVQEVMKDLRVVDCDILTIGQYLQPSLKHLP
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
VDTFIKPEQFDQWKKIGLSMGFLQVVASPLTRSSYHAEEVKELMVNYPKTKS
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVVKPDWLRVKAPQWQRVGTVKEILRDLSLKTVCEEASCPNIGECFNAGTATFLIMGPAC
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEEECCHH
TRACPYCDINFEKKPQPLDVNEPKQLAEAVKRLRLNHVVITSVNRDDLTDGGATQFAKCI
HCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHH
SQVRAISPQTTIEVLIPDLCGNWQALEIILNAKPEVINHNTETVQRLYRRVRPQGNYHRT
HHHHHCCCCCHHEEEEHHHCCCCEEEEEEECCCCHHHCCCHHHHHHHHHHHCCCCCHHHH
LELLKRARELAPEIYTKSGIMVGVGETDAEVQEVMKDLRVVDCDILTIGQYLQPSLKHLP
HHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
VDTFIKPEQFDQWKKIGLSMGFLQVVASPLTRSSYHAEEVKELMVNYPKTKS
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]