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The map label for this gene is queA [H]

Identifier: 113474574

GI number: 113474574

Start: 1181432

End: 1182541

Strand: Reverse

Name: queA [H]

Synonym: Tery_0731

Alternate gene names: 113474574

Gene position: 1182541-1181432 (Counterclockwise)

Preceding gene: 113474576

Following gene: 113474572

Centisome position: 15.26

GC content: 37.21

Gene sequence:

>1110_bases
ATGACCGATTTAGATTGGTCCATTGAGGCTTATGATTATGAACTTCCCCAAGAGCTAATTGCTCAAAATCCAGTTGTTCC
TAGAGACAGCTCTCGCTTAATGGTTATAGAGTCCCTCACACACAATCATCGTATTTTCAGGGATTTACCAGATTTACTCC
AACCAGAAGATTTACTAGTATTAAATAATACCCGTGTGATTCCCGCTAGGCTTTATGGTCAAAAAACGAGTGGTGCTCAG
GTAGAAATATTATTATTGAAACAGATCACAAATGATTCTTGGTTAGCTTTGGTCAAGCCTGGCAAACATTTTAGTGCTGG
GACAAGGATTGAGTTTGAACCTAATTCATCATTTACTTGTGATTATAAGTTGCAGGCAACAGTATTAGCAATTGATCAAG
AAACAGGTGGACGTGTTTTACAATTTTATTGGCCTCAAGAAGTTTCTTGGCTTGATTTGTTAAAGCAATTTGGTCATATA
CCTTTTCCTCCTTATGTTACAGGTTCACAGGCTTTAACTGAACAATATCAAACTGTATATAGTAAGATAGCAGGAGCTAT
CGCTGCTCCTACCGCTGGCTTACATTTTACTGATGAATTACTATATAAACTAGAACAAGGGAATATTGGACGAGCTTTTA
TAACTTTGCACGTTGGTATTGGCACATTTCGTCCGGTTGAAGTTAAAGATATTACTACCCACAATATACACTCCGAATGG
GTTGAGGTTTCATCTTTGAGTGTAGATAAAATTCACCAGACAAAGGCTAAAGGTGGACGCATTATCGCAGTGGGAACCAC
AGTAGTGAGAGCTATAGAAGCAGCAGCGCTAGCTAATGATAATTATAGCACTCAAGAATCTTTAATTCCTTTAATTCGAC
CATTTTCTGATTACACAGATATATTTATTTATCCTGGTTACAAATGGCGGGTAGTAGATGGTTTAATCACTAATTTCCAT
CTTCCGCGCTCTAGTTTACTTATGCTTGTTAGTGCGATGATTGGTAGAAAACGTCTTTTAAGTTTATACCAGGATGCAAT
TGATCAAAAATACCGCTTTTATTCTTTTGGTGATGCAATGTTTATTCTACCATCGGCTAGAGTTAATTAA

Upstream 100 bases:

>100_bases
CCACTTTCAAAAAGATCAAAAAGTAAACCTAACCTAGGATTTTCTTAACTCCTGATGACAGGAGATAATGATAACTAATA
ACTGATAACTGATAACTGAA

Downstream 100 bases:

>100_bases
ATCAAATTATGTAGCGTGGGAATATTGCCTCCAACAGTCTACCTAACAAGCTTTTTTTAAATCTAGTGCATATTTTAGGA
GATTATTTTGGGACAATTAG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTDLDWSIEAYDYELPQELIAQNPVVPRDSSRLMVIESLTHNHRIFRDLPDLLQPEDLLVLNNTRVIPARLYGQKTSGAQ
VEILLLKQITNDSWLALVKPGKHFSAGTRIEFEPNSSFTCDYKLQATVLAIDQETGGRVLQFYWPQEVSWLDLLKQFGHI
PFPPYVTGSQALTEQYQTVYSKIAGAIAAPTAGLHFTDELLYKLEQGNIGRAFITLHVGIGTFRPVEVKDITTHNIHSEW
VEVSSLSVDKIHQTKAKGGRIIAVGTTVVRAIEAAALANDNYSTQESLIPLIRPFSDYTDIFIYPGYKWRVVDGLITNFH
LPRSSLLMLVSAMIGRKRLLSLYQDAIDQKYRFYSFGDAMFILPSARVN

Sequences:

>Translated_369_residues
MTDLDWSIEAYDYELPQELIAQNPVVPRDSSRLMVIESLTHNHRIFRDLPDLLQPEDLLVLNNTRVIPARLYGQKTSGAQ
VEILLLKQITNDSWLALVKPGKHFSAGTRIEFEPNSSFTCDYKLQATVLAIDQETGGRVLQFYWPQEVSWLDLLKQFGHI
PFPPYVTGSQALTEQYQTVYSKIAGAIAAPTAGLHFTDELLYKLEQGNIGRAFITLHVGIGTFRPVEVKDITTHNIHSEW
VEVSSLSVDKIHQTKAKGGRIIAVGTTVVRAIEAAALANDNYSTQESLIPLIRPFSDYTDIFIYPGYKWRVVDGLITNFH
LPRSSLLMLVSAMIGRKRLLSLYQDAIDQKYRFYSFGDAMFILPSARVN
>Mature_368_residues
TDLDWSIEAYDYELPQELIAQNPVVPRDSSRLMVIESLTHNHRIFRDLPDLLQPEDLLVLNNTRVIPARLYGQKTSGAQV
EILLLKQITNDSWLALVKPGKHFSAGTRIEFEPNSSFTCDYKLQATVLAIDQETGGRVLQFYWPQEVSWLDLLKQFGHIP
FPPYVTGSQALTEQYQTVYSKIAGAIAAPTAGLHFTDELLYKLEQGNIGRAFITLHVGIGTFRPVEVKDITTHNIHSEWV
EVSSLSVDKIHQTKAKGGRIIAVGTTVVRAIEAAALANDNYSTQESLIPLIRPFSDYTDIFIYPGYKWRVVDGLITNFHL
PRSSLLMLVSAMIGRKRLLSLYQDAIDQKYRFYSFGDAMFILPSARVN

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=356, Percent_Identity=46.0674157303371, Blast_Score=304, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 41674; Mature: 41543

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLDWSIEAYDYELPQELIAQNPVVPRDSSRLMVIESLTHNHRIFRDLPDLLQPEDLLV
CCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
LNNTRVIPARLYGQKTSGAQVEILLLKQITNDSWLALVKPGKHFSAGTRIEFEPNSSFTC
ECCCEEEEHHCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEECCCCCEEE
DYKLQATVLAIDQETGGRVLQFYWPQEVSWLDLLKQFGHIPFPPYVTGSQALTEQYQTVY
EEEEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
SKIAGAIAAPTAGLHFTDELLYKLEQGNIGRAFITLHVGIGTFRPVEVKDITTHNIHSEW
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEECCCCCCCHHHH
VEVSSLSVDKIHQTKAKGGRIIAVGTTVVRAIEAAALANDNYSTQESLIPLIRPFSDYTD
HHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCE
IFIYPGYKWRVVDGLITNFHLPRSSLLMLVSAMIGRKRLLSLYQDAIDQKYRFYSFGDAM
EEEECCEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEE
FILPSARVN
EEECCCCCC
>Mature Secondary Structure 
TDLDWSIEAYDYELPQELIAQNPVVPRDSSRLMVIESLTHNHRIFRDLPDLLQPEDLLV
CCCCCEEEEECCCCCHHHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
LNNTRVIPARLYGQKTSGAQVEILLLKQITNDSWLALVKPGKHFSAGTRIEFEPNSSFTC
ECCCEEEEHHCCCCCCCCCEEEEEEEEECCCCCEEEEECCCCCCCCCCEEEECCCCCEEE
DYKLQATVLAIDQETGGRVLQFYWPQEVSWLDLLKQFGHIPFPPYVTGSQALTEQYQTVY
EEEEEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
SKIAGAIAAPTAGLHFTDELLYKLEQGNIGRAFITLHVGIGTFRPVEVKDITTHNIHSEW
HHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCCCEEEECCCCCCCHHHH
VEVSSLSVDKIHQTKAKGGRIIAVGTTVVRAIEAAALANDNYSTQESLIPLIRPFSDYTD
HHHHCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCE
IFIYPGYKWRVVDGLITNFHLPRSSLLMLVSAMIGRKRLLSLYQDAIDQKYRFYSFGDAM
EEEECCEEEEEECHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEE
FILPSARVN
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA