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The map label for this gene is rhtB [H]

Identifier: 110835448

GI number: 110835448

Start: 2928083

End: 2928709

Strand: Reverse

Name: rhtB [H]

Synonym: ABO_2587

Alternate gene names: 110835448

Gene position: 2928709-2928083 (Counterclockwise)

Preceding gene: 110835449

Following gene: 110835447

Centisome position: 93.86

GC content: 39.71

Gene sequence:

>627_bases
ATGAATATTGAATTATATACTTTCTTTCTCGTTACAACGGTGATGCTTATTTTGGTTCCTGGGCCAGCGGCTATAACGGT
GGCGAAACAGGCTGCATCCCATAATTCAAAATTAACTTTTTTAAGTGTTCTTGGCGTAGCAAGCGCAGATGTATTATTTT
TTATATTGTCGGCAACAGGCATCGCATCGCTAATAATAGCGTCCAGTTTGGTATTTTCTATCATTAAGTGGTTTGGTATT
GCTTATCTTCTTTTCCTGGGTATTTCGGCAATTTTTAGCAAAGCAGGGCCAGTTAACGTTGATGTTCAATTAACCACAAT
GAATCCTGCGAAAGCTTTTTCAAATGGTCTCGTCATTCAATTAGCTAACCCAAAAGCGTTAATGTATTTTTCAGCCCTAT
TGCCGCAGTTTGTGGACCCTAAAGAGCCTGTCATTTTACAAATGGTTCTTATGGGGCTCACCTGTTTTATTGCTGATTTA
GTGGTTTACAGTCTGTTTGGCCGCATGGGGGCACAATTAGCAAGAAAACGAGTAAAGTCTTGGGTTATAAACCTTATCAA
TAAAGCAGCGGGTACGGCATTGATTGCCACCGGAATAAAAATGGTAACGTTGGGAAGTACAAAATAG

Upstream 100 bases:

>100_bases
GAGTTTGTCGATTTTTATAATCGGGAGCGGTTTCACTCTGGGATTGGGTACCAAAGTCCGGCAAGCTATGTGCGGGCGTT
AGGCTAAATGAAGGGTAAGC

Downstream 100 bases:

>100_bases
CCGCCATCAAACATTTTCGCGTGAGCCGGGAGTGATGCTTCAGGAGACGTTAAGTGCCATATAGGATGAACGAAAAACAG
TTCGACGCCGTCTTGGCGCT

Product: amino acid transporter LysE

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNIELYTFFLVTTVMLILVPGPAAITVAKQAASHNSKLTFLSVLGVASADVLFFILSATGIASLIIASSLVFSIIKWFGI
AYLLFLGISAIFSKAGPVNVDVQLTTMNPAKAFSNGLVIQLANPKALMYFSALLPQFVDPKEPVILQMVLMGLTCFIADL
VVYSLFGRMGAQLARKRVKSWVINLINKAAGTALIATGIKMVTLGSTK

Sequences:

>Translated_208_residues
MNIELYTFFLVTTVMLILVPGPAAITVAKQAASHNSKLTFLSVLGVASADVLFFILSATGIASLIIASSLVFSIIKWFGI
AYLLFLGISAIFSKAGPVNVDVQLTTMNPAKAFSNGLVIQLANPKALMYFSALLPQFVDPKEPVILQMVLMGLTCFIADL
VVYSLFGRMGAQLARKRVKSWVINLINKAAGTALIATGIKMVTLGSTK
>Mature_208_residues
MNIELYTFFLVTTVMLILVPGPAAITVAKQAASHNSKLTFLSVLGVASADVLFFILSATGIASLIIASSLVFSIIKWFGI
AYLLFLGISAIFSKAGPVNVDVQLTTMNPAKAFSNGLVIQLANPKALMYFSALLPQFVDPKEPVILQMVLMGLTCFIADL
VVYSLFGRMGAQLARKRVKSWVINLINKAAGTALIATGIKMVTLGSTK

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=162, Percent_Identity=29.0123456790123, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1788099, Length=138, Percent_Identity=29.7101449275362, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22218; Mature: 22218

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: PS00027 HOMEOBOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELYTFFLVTTVMLILVPGPAAITVAKQAASHNSKLTFLSVLGVASADVLFFILSATG
CCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IASLIIASSLVFSIIKWFGIAYLLFLGISAIFSKAGPVNVDVQLTTMNPAKAFSNGLVIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHCCCEEEE
LANPKALMYFSALLPQFVDPKEPVILQMVLMGLTCFIADLVVYSLFGRMGAQLARKRVKS
ECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVINLINKAAGTALIATGIKMVTLGSTK
HHHHHHHHHHCHHHHHCCEEEEEECCCC
>Mature Secondary Structure
MNIELYTFFLVTTVMLILVPGPAAITVAKQAASHNSKLTFLSVLGVASADVLFFILSATG
CCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IASLIIASSLVFSIIKWFGIAYLLFLGISAIFSKAGPVNVDVQLTTMNPAKAFSNGLVIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHCCCEEEE
LANPKALMYFSALLPQFVDPKEPVILQMVLMGLTCFIADLVVYSLFGRMGAQLARKRVKS
ECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WVINLINKAAGTALIATGIKMVTLGSTK
HHHHHHHHHHCHHHHHCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]